Library construction system for genetic detection

Abstract

The utility model discloses a kind of library construction systems for gene detection, it is related to detection technical field, library construction system includes: the extraction equipment of installation extraction warehouse;Library building equipment is installed in library building warehouse;Delivery warehouse is between extraction warehouse and library building warehouse, conveying mechanism is worn in delivery warehouse, extraction warehouse and library building warehouse are selectively communicated with delivery warehouse to make conveying mechanism with the sample to be measured in extraction warehouse through delivery warehouse and be sent to library building warehouse.The conveying mechanism is sent to library building warehouse with the sample to be measured in extraction warehouse through delivery warehouse by being set with delivery device, extraction warehouse and library building warehouse can be set in same laboratory area, effectively reduce the floor area of high-throughput sequencing laboratory, save the construction cost of high-throughput sequencing laboratory and save gene detection product landing time, and, realize the full-process automation of extraction link to library building link, effectively prevent the detection result error caused by artificial operation mistake, improve the detection result accuracy.

Description

[0001] Cross-reference to related applications

[0002] This application is based on and claims priority to Chinese Patent Application No. 202510455077.6, filed on April 11, 2025, the entire contents of which are incorporated herein by reference. Technical Field

[0003] This utility model relates to the field of detection technology, and in particular to a library construction system for gene detection. Background Technology

[0004] In related technologies, high-throughput sequencing laboratories (NGS) set up extraction and library preparation equipment in separate laboratory areas to avoid cross-contamination of samples. However, setting up extraction and library preparation equipment in different laboratory areas leads to high construction costs, long construction time, and large required land area for high-throughput sequencing laboratories. Furthermore, the process connection between the extraction and library preparation stages requires professional personnel to operate, which cannot effectively prevent errors in test results caused by human error. Utility Model Content

[0005] This invention aims to solve at least one of the technical problems existing in the prior art. Therefore, one objective of this invention is to provide a library construction system for gene detection that reduces the floor space required for high-throughput sequencing laboratories, saves construction costs and time, and improves the accuracy of detection results.

[0006] The library construction system for gene detection according to this utility model includes: an extraction chamber, in which an extraction device is installed for extracting a sample to be tested; a library construction chamber, in which a library construction device is installed for converting the extracted sample to be tested into a standardized library that can be identified; the extraction chamber and the library construction chamber are arranged along a first direction and spaced apart; and a transfer device, which includes a transfer chamber and a conveying mechanism. The transfer chamber is disposed between the extraction chamber and the library construction chamber, and the conveying mechanism passes through the transfer chamber along the first direction, with one end of the conveying mechanism extending into the extraction chamber and the other end extending into the library construction chamber. Both the extraction chamber and the library construction chamber can be selectively connected to the transfer chamber so that the conveying mechanism transports the sample to be tested in the extraction chamber to the library construction chamber through the transfer chamber.

[0007] According to the present invention, a library construction system for gene detection is provided. By setting up a transfer device, the sample to be tested in the extraction chamber is transported to the library construction chamber through the transfer chamber by the delivery mechanism. The extraction chamber and the library construction chamber can be set up in the same laboratory area, which effectively reduces the floor space of the high-throughput sequencing laboratory. The library construction system can completely simulate the environment required for sample extraction and library construction, saving the construction cost of the high-throughput sequencing laboratory and the time required for gene detection products to be deployed. Furthermore, it realizes full automation of the process from extraction to library construction, effectively preventing errors in test results caused by human error and improving the accuracy of test results.

[0008] In some examples of this utility model, a first notch is formed on the side wall of the first extraction chamber opposite to the transfer chamber, and a second notch is formed on the side wall of the first extraction chamber opposite to the transfer chamber. The first notch and the second notch are opposite to and connected along a first direction. The second notch is selectively opened or closed to connect or separate the extraction chamber and the transfer chamber. When the second notch is open, the conveying mechanism transports the sample to be tested to the transfer chamber through the first notch and the second notch.

[0009] In some examples of this utility model, a first sealing door is movably provided on the first sidewall, which is used to open or close the second notch.

[0010] In some examples of this utility model, the first sealing door is slidably disposed on the first side wall along the second direction to open or close the second notch, and the first direction and the second direction are perpendicular.

[0011] In some examples of this utility model, a first through hole is formed on the side wall of the first extraction chamber. The first through hole and the first notch are adjacent to and connected along the second direction. A second through hole is formed on the first side wall. The second through hole and the second notch are adjacent to and connected along the second direction. The first through hole and the second through hole are opposite to and connected along the first direction. The conveying mechanism passes through the first through hole and the second through hole. The first direction and the second direction are perpendicular.

[0012] In some examples of this utility model, the first sealing door has an abutting end, and the abutting end is fixedly provided with a first sealing element. When the first sealing door closes the second notch, the first sealing element abuts and seals with the conveying mechanism.

[0013] In some examples of this utility model, along the third direction, the two side walls of the conveying mechanism are sealed to the transfer chamber and the extraction chamber, and the first direction, the second direction and the third direction are perpendicular to each other.

[0014] In some examples of this utility model, a second sealing member is fixed between the first sidewall and the first extraction chamber sidewall. The second sealing member abuts against both the first sidewall and the first extraction chamber sidewall. The second sealing member is arranged around the second notch along the circumference of the second notch, and the second sealing member is arranged around the first notch along the circumference of the first notch.

[0015] In some examples of this utility model, a third sealing element is fixed between the first sidewall and the first extraction chamber sidewall. The third sealing element abuts against both the first sidewall and the first extraction chamber sidewall. The third sealing element is located on the side of the second sealing element away from the first notch. The third sealing element is arranged around the second sealing element along its circumference.

[0016] In some examples of this utility model, the third sealing element has a first sub-sealing strip, a second sub-sealing strip, and a third sub-sealing strip. The second sub-sealing strip is connected between the first sub-sealing strip and the third sub-sealing strip. The first sub-sealing strip and the third sub-sealing strip both extend along the second direction and are respectively disposed on two opposite side edges of the first side wall along the third direction. Along the second direction, the second sub-sealing strip is disposed on the upper edge of the first side wall. The first direction, the second direction, and the third direction are perpendicular to each other.

[0017] In some examples of this utility model, the third sealing element also has a fourth sub-sealing strip and a fifth sub-sealing strip. Along the third direction, the fourth sub-sealing strip and the fifth sub-sealing strip are respectively located on both sides of the conveying mechanism. Along the second direction, the lower end of the first sub-sealing strip is connected to the fourth sub-sealing strip, and the lower end of the third sub-sealing strip is connected to the fifth sub-sealing strip. The fourth sub-sealing strip and the fifth sub-sealing strip both extend along the third direction and are disposed on the lower edge of the first side wall. The fourth sub-sealing strip and the fifth sub-sealing strip respectively abut against and seal with the two side walls of the conveying mechanism.

[0018] In some examples of this utility model, the warehouse construction warehouse has a first warehouse construction warehouse sidewall opposite to the transfer warehouse, the transfer warehouse has a second sidewall opposite to the first warehouse construction warehouse sidewall, the first warehouse construction warehouse sidewall and the first extraction warehouse sidewall have the same structure, the second sidewall and the first sidewall have the same structure, and the assembly method of the first warehouse construction warehouse sidewall and the second sidewall is the same as the assembly method of the first extraction warehouse sidewall and the first sidewall.

[0019] In some examples of this utility model, at least one side wall of the extraction compartment is provided with a first compartment door for opening or closing the extraction compartment, and / or at least one side wall of the construction compartment is provided with a second compartment door for opening or closing the construction compartment.

[0020] In some examples of this utility model, the first compartment door and the second compartment door are the same.

[0021] In some examples of this utility model, both the first compartment door and the second compartment door include: a door frame and a movable door. The door frame has a pick-up and put-out hole, and the movable door is movably disposed on the door frame to open or close the pick-up and put-out hole.

[0022] In some examples of this utility model, the movable door and the door frame are arranged opposite to each other and are slidably disposed on the door frame along the second direction, which is perpendicular to the first direction. A fourth sealing member is fixedly provided on the surface of the door frame facing the movable door, and the fourth sealing member abuts against the movable door. A fifth sealing member is fixedly provided on the surface of the movable door facing the door frame, and the fifth sealing member abuts against the door frame. When the movable door closes the pick-up and drop-off hole, the fourth sealing member and the fifth sealing member are arranged around the pick-up and drop-off hole.

[0023] In some examples of this utility model, the fourth sealing element includes: a sixth sub-sealing strip and two seventh sub-sealing strips. Along the second direction, the sixth sub-sealing strip is located at the upper edge of the door frame, and the two seventh sub-sealing strips extend along the second direction and are respectively located at the two side edges of the door frame. The sixth sub-sealing strip is connected between the two fifth sub-sealing strips. The fifth sealing element includes: an eighth sub-sealing strip and two ninth sub-sealing strips. Along the second direction, the eighth sub-sealing strip is located at the lower edge of the movable door, and the two ninth sub-sealing strips extend along the second direction and are respectively located at the two side edges of the movable door. The eighth sub-sealing strip is connected between the two ninth sub-sealing strips. The seventh sub-sealing strips and the corresponding ninth sub-sealing strips are arranged along the width direction of the pick-up and drop-out hole.

[0024] In some examples of this utility model, the air pressure inside the extraction chamber is greater than the air pressure inside the construction chamber.

[0025] In some examples of this utility model, the air pressure inside the transfer chamber is less than the air pressure inside the extraction chamber but greater than the air pressure inside the construction chamber.

[0026] In some examples of this utility model, the extraction chamber has a first gas driving component and a first filter element. The first gas driving component is used to drive gas into and out of the extraction chamber to regulate the gas pressure inside the extraction chamber, and the first filter element is used to filter the gas flowing into the extraction chamber.

[0027] In some examples of this utility model, the gas-building chamber has a second gas driving component and a second filter element. The second gas driving component is used to drive gas into and out of the gas-building chamber to regulate the gas pressure inside the gas-building chamber, and the second filter element is used to filter the gas flowing into the gas-building chamber.

[0028] In some examples of this utility model, the conveying mechanism includes a driving mechanism, a guide rail, and a placement platform. The guide rail passes through the transfer chamber along a first direction, and both ends of the guide rail extend into the extraction chamber and the silo building chamber, respectively. The placement platform is located outside the guide rail and is used to place the sample to be tested. At least part of the driving mechanism is located inside the guide rail and is connected to the placement platform in a transmission manner. The driving mechanism is used to drive the placement platform to reciprocate relative to the guide rail along the first direction, so that the placement platform moves into one of the extraction chamber, the silo building chamber, and the transfer chamber.

[0029] In some examples of this utility model, the guide rail has a guide rail wall facing the placement platform, the guide rail wall is formed with a clearance hole extending along a first direction, the drive mechanism includes a drive rod passing through the clearance hole, the clearance hole is provided with a sixth sealing member, the sixth sealing member is used to seal the clearance hole, the sixth sealing member includes a first sealing strip and a second sealing strip, the first sealing strip and the second sealing strip both extend along the first direction and are located on both sides of the drive rod, the first sealing strip and the second sealing strip both abut against the drive rod for sealing.

[0030] Additional aspects and advantages of this invention will be set forth in part in the description which follows, and in part will be obvious from the description, or may be learned by practice of the invention. Attached Figure Description

[0031] The above and / or additional aspects and advantages of this utility model will become apparent and readily understood from the description of the embodiments taken in conjunction with the following drawings, in which:

[0032] Figure 1 This is a schematic diagram of the library construction system according to an embodiment of the present utility model;

[0033] Figure 2 This is a schematic diagram of the structure of the transfer device according to an embodiment of the present utility model;

[0034] Figure 3 yes Figure 2 Enlarged view of point A;

[0035] Figure 4 This is an exploded view of the first compartment door according to an embodiment of the present utility model;

[0036] Figure 5 This is a structural schematic diagram of the first compartment door (hidden movable door) according to an embodiment of the present utility model;

[0037] Figure 6 This is a schematic diagram of the conveying mechanism according to an embodiment of the present utility model;

[0038] Figure 7 This is a structural schematic diagram of the transfer device according to an embodiment of the present utility model from another angle.

[0039] Figure label:

[0040] Document building system 100;

[0041] Extraction chamber 1; First extraction chamber sidewall 11; First notch 111; First through hole 112; Second seal 113;

[0042] Third sealing element 114; First sub-sealing strip 1141; Second sub-sealing strip 1142; Third sub-sealing strip 1143; Fourth sub-sealing strip 1144;

[0043] First compartment door 12; Second compartment door 13; Door frame 121; Fourth sealing element 1211; Sixth sub-sealing strip 1212; Seventh sub-sealing strip 1213;

[0044] 122 movable door; 1221 fifth seal; 1222 eighth sub-seal strip; 1223 ninth sub-seal strip; 123 take-up / removal hole; 13 first gas drive assembly; 131 first sub-gas drive assembly; 14 first filter element;

[0045] Storage compartment 2; second gas drive assembly 21; second sub-gas drive component 211; second filter component 22;

[0046] Transfer device 3; transfer chamber 31; first side wall 311; second notch 312; first sealing door 313; abutting end 315; first sealing element 316; second side wall 317;

[0047] Conveying mechanism 32; Conveying mechanism side wall 321; Drive mechanism 322; Drive rod 3221; Drive motor 3222; Transmission belt 3223; Connector 3224; Guide rail 323; Guide rail wall 3231; Pressure strip 3232; Sixth sealing element 3233; First sealing strip 3234; Second sealing strip 3235; Fixed base 3236; Placement platform 324. Detailed Implementation

[0048] The embodiments of this utility model are described in detail below. Examples of these embodiments are shown in the accompanying drawings, wherein the same or similar reference numerals denote the same or similar elements or elements having the same or similar functions throughout. The embodiments described below with reference to the accompanying drawings are exemplary and are only used to explain this utility model, and should not be construed as limiting this utility model.

[0049] The following is for reference. Figures 1-7 A library construction system 100 according to an embodiment of the present utility model is described.

[0050] like Figures 1-2As shown, the library construction system 100 according to this utility model includes: an extraction chamber 1, in which an extraction device is installed, for extracting a sample to be tested; a library construction chamber 2, in which a library construction device is installed, for converting the extracted sample to be tested into a standardized library that can be identified; the extraction chamber 1 and the library construction chamber 2 are arranged along a first direction and spaced apart; and a transfer device 3, which includes a transfer chamber 31 and a conveying mechanism 32. The transfer chamber 31 is disposed between the extraction chamber 1 and the library construction chamber 2, and the conveying mechanism 32 passes through the transfer chamber 31 along the first direction. One end of the conveying mechanism 32 extends into the extraction chamber 1, and the other end of the conveying mechanism 32 extends into the library construction chamber 2. Both the extraction chamber 1 and the library construction chamber 2 can be selectively connected to the transfer chamber 31 so that the conveying mechanism 32 transports the sample to be tested in the extraction chamber 1 to the library construction chamber 2 through the transfer chamber 31.

[0051] The extraction chamber 1 contains an extraction device for nucleic acid extraction. This device can be installed in the extraction chamber 1 using methods such as bolts or snap-fits. The extraction device is used to extract the sample to be tested. The library construction chamber 2 contains a library construction device for constructing sequencing libraries. This device can be installed in the library construction chamber 2 using methods such as bolts or snap-fits. The library construction device is used to convert the extracted sample into a standardized, identifiable library.

[0052] The first direction is... Figure 2 In the X direction, extraction chamber 1 and construction chamber 2 are arranged along the first direction and spaced apart to reduce the risk of cross-contamination. Along the first direction, transfer chamber 31 is located between extraction chamber 1 and construction chamber 2, and conveying mechanism 32 passes through transfer chamber 31 along the first direction. This arrangement allows for a reasonable configuration of the conveying mechanism 32, enabling one end of the conveying mechanism 32 to extend into extraction chamber 1 and the other end into construction chamber 2.

[0053] Sealed doors can be installed between extraction chamber 1 and transfer chamber 31, and between collection chamber 2 and transfer chamber 31. When the corresponding sealed door is opened, the corresponding extraction chamber 1 or the corresponding collection chamber 2 is connected to the transfer chamber 31. When the corresponding sealed door is closed, the corresponding extraction chamber 1 or the corresponding collection chamber 2 is not connected to the transfer chamber 31. This arrangement allows both extraction chamber 1 and collection chamber 2 to be selectively connected to the transfer chamber 31, so that the conveying mechanism 32 can transport the sample to be tested in extraction chamber 1 to collection chamber 2 through the transfer chamber 31.

[0054] By setting up a transfer device 3 so that the delivery mechanism 32 can transport the sample to be tested in the extraction chamber 1 to the library construction chamber 2 through the transfer chamber 31, the extraction chamber 1 and the library construction chamber 2 can be set up in the same laboratory area, so that the library construction system 100 occupies a smaller area. For example, this way the library construction system 100 occupies an area of ​​about 6 square meters (including the detection equipment and gene analysis integrated machine), while setting the extraction equipment and library construction equipment in different laboratory areas occupies an area of ​​about 80 square meters. It can be seen that the library construction system 100 effectively reduces the floor space of the high-throughput sequencing laboratory, saves the construction cost and time of the high-throughput sequencing laboratory. Furthermore, the delivery mechanism 32 can automate the process between the extraction and library construction stages by transporting the sample to be tested in the extraction chamber 1 to the library construction chamber 2 through the transfer chamber 31, effectively preventing errors in the test results caused by human operation errors, improving the standardization, normalization and convenience of gene testing, and thus improving the accuracy of the test results.

[0055] Specifically, the extraction equipment is installed in the extraction chamber 1, and the silo building equipment is installed in the silo building chamber 2. The extraction chamber 1 and the silo building chamber 2 are arranged along a first direction and are spaced apart. The transfer chamber 31 is installed in the gap between the extraction chamber 1 and the silo building chamber 2, so that both the extraction chamber 1 and the silo building chamber 2 can selectively communicate with the transfer chamber 31. The conveying mechanism 32 passes through the transfer chamber 31, with one end of the conveying mechanism 32 extending into the extraction chamber 1 and the other end of the conveying mechanism 32 extending into the silo building chamber 2.

[0056] The library construction system 100 is equipped with a detection program. When the sample to be tested needs to be transferred from extraction chamber 1 to library construction chamber 2 via transfer chamber 31, the air pressure in extraction chamber 1 is greater than the air pressure in library construction chamber 2. This application uses an example where the air pressure in library construction chamber 2 is 5 Pa and the air pressure in extraction chamber 1 is 10 Pa. The sample to be tested and the reagents and consumables required for the extraction equipment are placed in extraction chamber 1, and the reagents and consumables required for library construction are placed in library construction chamber 2. The extraction equipment in extraction chamber 1 extracts the sample to be tested. After extraction, the detection program first controls the connection between extraction chamber 1 and transfer chamber 31. It should be noted that at this time, library construction chamber 2 and transfer chamber 31 are not connected. The air pressure in extraction chamber 1 and transfer chamber 31 will quickly exchange and reach equilibrium, and eventually both will drop to slightly below 10 Pa (at this time, the air pressure in library construction chamber 2 is still about 5 Pa).

[0057] At the same time, the detection program controls the conveying mechanism 32 to transport the sample to be tested after extraction in the extraction chamber 1 to the transfer chamber 31. After the sample to be tested has completely entered the transfer chamber 31, the detection program controls the extraction chamber 1 and the transfer chamber 31 to be disconnected, while the storage chamber 2 and the transfer chamber 31 are connected. Based on the same principle, the air pressure in the transfer chamber 31 and the storage chamber 2 will be rapidly exchanged and reach equilibrium, and eventually the overall pressure will rise to slightly higher than 5 Pa (at this time, the air pressure in the extraction chamber 1 will return to about 10 Pa).

[0058] During this process, the air in the transfer chamber 31 and the preparation chamber 2 does not flow to the extraction chamber 1. After the detection program controls the conveying mechanism 32 to continue transporting the sample to be tested from the transfer chamber 31 to the preparation chamber 2, the detection program controls the preparation chamber 2 and the transfer chamber 31 to be disconnected. The preparation equipment in the preparation chamber 2 is used to convert the extracted sample into an identifiable standardized library. Throughout the entire process, the airflow always flows from the extraction chamber 1 through the transfer chamber 31 to the preparation chamber 2, with a single flow direction, effectively reducing the risk of cross-contamination of gases in the extraction chamber 1 and the preparation chamber 2.

[0059] Therefore, by setting up the transfer device 3 so that the conveying mechanism 32 can transport the sample to be tested in the extraction chamber 1 to the library construction chamber 2 through the transfer chamber 31, the extraction chamber 1 and the library construction chamber 2 can be set up in the same laboratory area, effectively reducing the floor space of the high-throughput sequencing laboratory. The library construction system 100 can completely simulate the environment required for sample extraction and library construction, saving the construction cost of the high-throughput sequencing laboratory and the time required for the rollout of gene detection products. Furthermore, it automates the process between the extraction and library construction stages, effectively preventing errors in test results caused by human error and improving the accuracy of test results.

[0060] In some examples of this utility model, such as Figure 1 and Figure 2 As shown, the first extraction chamber sidewall 11 opposite to the transfer chamber 31 has a first notch 111, and the first sidewall 311 opposite to the first extraction chamber sidewall 11 has a second notch 312. The first notch 111 and the second notch 312 are opposite to each other and connected along a first direction. The second notch 312 is selectively opened or closed to connect or separate the extraction chamber 1 and the transfer chamber 31. When the second notch 312 is open, the conveying mechanism 32 transports the sample to be tested to the transfer chamber 31 through the first notch 111 and the second notch 312.

[0061] The extraction chamber 1 has a first extraction chamber sidewall 11, which is a sidewall opposite to the extraction chamber 1 and the transfer chamber 31. The first extraction chamber sidewall 11 has a first notch 111. The transfer chamber 31 has a first sidewall 311, which is a sidewall opposite to the first extraction chamber sidewall 11. The first sidewall 311 has a second notch 312. The first notch 111 and the second notch 312 are opposite to each other along a first direction and are designed to be connected so that when the first notch 111 and the second notch 312 are connected, they can smoothly avoid the conveying mechanism 32, so that the conveying mechanism 32 can transport the sample to be tested in the extraction chamber 1 to the transfer chamber 31.

[0062] The second notch 312 can be selectively opened or closed to connect or separate the extraction chamber 1 and the transfer chamber 31. For example, the second notch 312 can be equipped with a sealing door. When the sealing door is open, the second notch 312 is open, and the conveying mechanism 32 can smoothly transport the sample to be tested to the transfer chamber 31 through the first notch 111 and the second notch 312. When the sealing door is closed, the second notch 312 is closed, reducing the risk of gas exchange between the extraction chamber 1 and the transfer chamber 31, thereby reducing the risk of cross-contamination of the sample to be tested.

[0063] In some examples of this utility model, such as Figure 2 As shown, the first sidewall 311 is movably provided with a first sealing door 313, which is used to open or close the second notch 312.

[0064] The first sidewall 311 is movably provided with a first sealing door 313, which is the sealing door of the above embodiment. In some embodiments of this application, the first sidewall 311 is slidably provided with the first sealing door 313. In some embodiments of this application, the first sidewall 311 is rotatably provided with the first sealing door 313. The first sealing door 313 is used to open or close the second notch 312. When the first sealing door 313 is open, the second notch 312 is open, and the conveying mechanism 32 can smoothly transport the sample to be tested to the transfer chamber 31 through the first notch 111 and the second notch 312. When the first sealing door 313 is closed, the second notch 312 is closed, reducing the risk of gas exchange between the extraction chamber 1 and the transfer chamber 31, thereby reducing the risk of cross-contamination of the sample to be tested. By movably providing a first sealing door 313 on the first side wall 311, the difficulty of opening and closing the second notch 312 can be reduced, thereby facilitating the transport of the sample to be tested and enabling the transport mechanism 32 to selectively transport the sample to be tested to the transfer chamber 31 through the first notch 111 and the second notch 312.

[0065] In some examples of this utility model, such as Figure 2 As shown, the first sealing door 313 is slidably disposed on the first side wall 311 along the second direction to open or close the second notch 312, and the first direction and the second direction are perpendicular.

[0066] Among them, along the second direction, that is Figure 2 In the Y direction, which is the height direction or the up-down direction of the library construction system 100, the first sealing door 313 is slidably disposed on the first side wall 311 to open or close the second notch 312. The first direction and the second direction are perpendicular, that is, the X direction and the Y direction are perpendicular.

[0067] Specifically, along the second direction, when the first sealing door 313 slides upward, it opens, and the second notch 312 opens, allowing the conveying mechanism 32 to smoothly transport the sample to be tested to the transfer chamber 31 through the first notch 111 and the second notch 312. Along the second direction, when the first sealing door 313 slides downward, it closes, and the second notch 312 closes, reducing the risk of gas exchange between the extraction chamber 1 and the transfer chamber 31, thereby reducing the risk of cross-contamination of the sample to be tested.

[0068] By setting a first sealing door 313 that can slide along the second direction, the risk of the first sealing door 313 blowing gas from the transfer chamber 31 into the extraction chamber 1 can be reduced during the opening or closing of the first sealing door 313, thereby reducing the risk of the sample to be tested in the extraction chamber 1 being contaminated, and further reducing the risk of cross-contamination of the sample to be tested.

[0069] In some examples of this utility model, such as Figure 1 and Figure 2 As shown, the first extraction chamber sidewall 11 also has a first through hole 112, the first through hole 112 and the first notch 111 are adjacent and connected along the second direction, the first sidewall 311 has a second through hole, the second through hole and the second notch 312 are adjacent and connected along the second direction, the first through hole 112 and the second through hole are opposite and connected along the first direction, and the conveying mechanism 32 passes through the first through hole 112 and the second through hole, the first direction and the second direction are perpendicular.

[0070] The first extraction chamber sidewall 11 also has a first through hole 112. Along the second direction, the first through hole 112 and the first notch 111 are adjacent to each other and are connected. The first sidewall 311 has a second through hole. Along the second direction, the second through hole and the second notch 312 are adjacent to each other and are connected. The first direction and the second direction are perpendicular, that is, the X direction and the Y direction are perpendicular.

[0071] Along the first direction, the first through hole 112 and the second through hole are arranged opposite to each other and are connected. Both the first through hole 112 and the second through hole are used to avoid the conveying mechanism 32, so that the conveying mechanism 32 can pass through the first through hole 112 and the second through hole, so that one end of the conveying mechanism 32 along the first direction can pass through the first side wall 311 of the transfer chamber 31, and one end of the conveying mechanism 32 extends into the extraction chamber 1, thereby realizing the effect that the conveying mechanism 32 can transport the sample to be tested in the extraction chamber 1 to the transfer chamber 31.

[0072] In some examples of this utility model, such as Figure 2 and Figure 3As shown, the first sealing door 313 has an abutment end 315, and the abutment end 315 is fixedly provided with a first sealing member 316. When the first sealing door 313 closes the second notch 312, the first sealing member 316 abuts and seals with the conveying mechanism 32.

[0073] The first sealing door 313 has an abutment end 315. Along the second direction, the lower end of the first sealing door 313 is the abutment end 315. The abutment end 315 is fixedly provided with a first sealing element 316. For example, the first sealing element 316 can be adhered to the abutment end 315, or the first sealing element 316 can be snapped onto the abutment end 315. The first sealing element 316 can be, but is not limited to, made of materials such as rubber, silicone, or foam. When the first sealing door 313 closes the second notch 312, the first sealing element 316 abuts and seals with the conveying mechanism 32 to smoothly seal the gap between the conveying mechanism 32 and the first sealing door 313, reducing the risk of gas exchange between the extraction chamber 1 and the transfer chamber 31 when the first sealing door 313 closes the second notch 312, thereby reducing the risk of contamination of the sample to be tested in the extraction chamber 1.

[0074] In some examples of this utility model, such as Figure 2 As shown, along the third direction, the two conveying mechanism sidewalls 321 of the conveying mechanism 32 are sealed to the transfer chamber 31 and the extraction chamber 1, and the first direction, the second direction and the third direction are perpendicular to each other.

[0075] Among them, along the third direction, that is Figure 2 In the Z direction, the conveying mechanism 32 has two conveying mechanism sidewalls 321, both of which are sealed to the transfer chamber 31 and the extraction chamber 1. For example, foam, silicone, rubber, etc. can be placed in the gap between the two conveying mechanism sidewalls 321 and the transfer chamber 31 and the extraction chamber 1 to achieve a sealing effect between the two conveying mechanism sidewalls 321 and the transfer chamber 31 and the extraction chamber 1. This reduces the risk of external air entering the transfer chamber 31 or the extraction chamber 1 through the gap between the conveying mechanism 32 and the transfer chamber 31 or the gap between the conveying mechanism 32 and the extraction chamber 1 during sample transport, thereby reducing the risk of contamination of the sample during transport and improving the accuracy of the test results.

[0076] In some examples of this utility model, such as Figure 2 As shown, a second sealing member 113 is fixed between the first sidewall 311 and the first extraction chamber sidewall 11. The second sealing member 113 abuts against both the first sidewall 311 and the first extraction chamber sidewall 11. The second sealing member 113 is arranged around the second notch 312 in the circumferential direction, and also around the first notch 111 in the circumferential direction.

[0077] A second sealing element 113 is fixed between the first sidewall 311 and the first extraction chamber sidewall 11. The second sealing element 113 may be made of materials such as rubber, silicone, or foam. The second sealing element 113 abuts against both the first sidewall 311 and the first extraction chamber sidewall 11. As some embodiments of this application, one end of the second sealing element 113 along the first direction may be provided with a double-sided adhesive strip. The end of the second sealing element 113 with the double-sided adhesive strip is bonded to the first sidewall 311, and the end of the second sealing element 113 without the double-sided adhesive strip abuts against the first extraction chamber sidewall 11, so that the second sealing element 113 seals the gap between the first sidewall 311 and the first extraction chamber sidewall 11.

[0078] As some embodiments of this application, the second sealing member 113 may be provided with double-sided adhesive strips at both ends along the first direction, and the two ends of the second sealing member 113 along the first direction are respectively bonded to the first side wall 311 and the first extraction chamber side wall 11, so that the second sealing member 113 seals the gap between the first side wall 311 and the first extraction chamber side wall 11. The second sealing member 113 is arranged around the second notch 312 circumferentially, and the second sealing member 113 is arranged around the first notch 111 circumferentially. This arrangement can make the seal between the extraction chamber 1 and the transfer chamber 31 reliable. During the opening of the first sealing door 313, the risk of external air entering the extraction chamber 1 or the transfer chamber 31 through the gap between the extraction chamber 1 and the transfer chamber 31 can be reduced, further reducing the risk of the test sample being contaminated during transportation, and further improving the accuracy of the test results.

[0079] In some examples of this utility model, such as Figure 2 As shown, a third sealing element 114 is fixed between the first sidewall 311 and the first extraction chamber sidewall 11. The third sealing element 114 abuts against both the first sidewall 311 and the first extraction chamber sidewall 11. The third sealing element 114 is located on the side of the second sealing element 113 away from the first notch 111. The third sealing element 114 is arranged around the second sealing element 113 along the circumference of the second sealing element 113.

[0080] A third sealing element 114 is fixed between the first sidewall 311 and the first extraction chamber sidewall 11. The third sealing element 114 may be made of materials such as rubber, silicone, or foam. The third sealing element 114 abuts against both the first sidewall 311 and the first extraction chamber sidewall 11. As some embodiments of this application, one end of the third sealing element 114 along the first direction may be provided with a double-sided adhesive strip. The end of the third sealing element 114 with the double-sided adhesive strip is bonded to the first sidewall 311, and the end of the third sealing element 114 without the double-sided adhesive strip abuts against the first extraction chamber sidewall 11, so that the third sealing element 114 seals the gap between the first sidewall 311 and the first extraction chamber sidewall 11.

[0081] As some embodiments of this application, the third sealing member 114 may be provided with double-sided adhesive strips at both ends along the first direction, and the third sealing member 114 is bonded to the first side wall 311 and the first extraction chamber side wall 11 at both ends along the first direction, so that the third sealing member 114 seals the gap between the first side wall 311 and the first extraction chamber side wall 11.

[0082] The third seal 114 is located on the side of the second seal 113 opposite to the first notch 111, and the third seal 114 is arranged around the second seal 113 circumferentially. This arrangement makes the seal between the extraction chamber 1 and the transfer chamber 31 more reliable. During the opening of the first sealing door 313, the second seal 113 and the third seal 114 jointly seal between the first side wall 311 and the first extraction chamber side wall 11, which greatly prevents ambient air from entering the library construction system 100 through the gap between the first side wall 311 and the first extraction chamber side wall 11, further reducing the risk of contamination of the test sample during transportation and further improving the accuracy of the test results.

[0083] In some examples of this utility model, such as Figure 2 As shown, the third sealing element 114 has a first sub-sealing strip 1141, a second sub-sealing strip 1142 and a third sub-sealing strip 1143. The second sub-sealing strip 1142 is connected between the first sub-sealing strip 1141 and the third sub-sealing strip 1143. The first sub-sealing strip 1141 and the third sub-sealing strip 1143 both extend along the second direction and are respectively disposed on two opposite side edges of the first side wall 311 along the third direction. Along the second direction, the second sub-sealing strip 1142 is disposed on the upper edge of the first side wall 311. The first direction, the second direction and the third direction are perpendicular to each other.

[0084] The second sub-sealing strip 1142 connects the first sub-sealing strip 1141 and the third sub-sealing strip 1143. Further, both the first and third sub-sealing strips 1141 and 1143 extend along the second direction and are respectively positioned on opposite sides of the first sidewall 311 along the third direction. The second sub-sealing strip 1142 extends along the third direction and is positioned on the upper edge of the first sidewall 311. This arrangement makes the third sealing element 114 more rationally positioned, facilitating reliable sealing of the gap between the first sidewall 311 and the first extraction chamber sidewall 11, thus improving the sealing effect of the third sealing element 114. The first, second, and third directions are perpendicular to each other; that is, the X, Y, and Z directions are perpendicular to each other.

[0085] In some examples of this utility model, such as Figure 2 and Figure 3 As shown, the third sealing element 114 also has a fourth sub-sealing strip 1144 and a fifth sub-sealing strip. Along the third direction, the fourth sub-sealing strip 1144 and the fifth sub-sealing strip are located on both sides of the conveying mechanism 32. Along the second direction, the lower end of the first sub-sealing strip 1141 is connected to the fourth sub-sealing strip 1144, and the lower end of the third sub-sealing strip 1143 is connected to the fifth sub-sealing strip. The fourth sub-sealing strip 1144 and the fifth sub-sealing strip both extend along the third direction and are disposed on the lower edge of the first side wall 311. The fourth sub-sealing strip 1144 and the fifth sub-sealing strip abut against and seal against the two conveying mechanism side walls 321 of the conveying mechanism 32.

[0086] Among them, along the third direction, that is Figure 2 In the Z direction, the first sub-sealing strip 1141 and the third sub-sealing strip 1143 are located on both sides of the conveying mechanism 32. In the second direction, the lower end of the first sub-sealing strip 1141 is connected to the fourth sub-sealing strip 1144, and the lower end of the third sub-sealing strip 1143 is connected to the fifth sub-sealing strip, so that the fourth sub-sealing strip 1144 and the fifth sub-sealing strip are located on both sides of the conveying mechanism 32, and both the fourth sub-sealing strip 1144 and the fifth sub-sealing strip extend in the third direction. Both the fourth sub-sealing strip 1144 and the fifth sub-sealing strip are set at the lower edge of the first side wall 311. The fourth sub-sealing strip 1144 and the fifth sub-sealing strip abut against and seal with the two conveying mechanism side walls 321 of the conveying mechanism 32. This arrangement makes the arrangement of the third sealing element 114 more reasonable, which is conducive to sealing the gap between the first side wall 311 and the first extraction chamber side wall 11 at the outer edge of the first side wall 311, and improving the sealing effect of the third sealing element 114.

[0087] In some examples of this utility model, such as Figure 1 As shown, the warehouse 2 has a first warehouse sidewall opposite to the transfer warehouse 31, and the transfer warehouse 31 has a second sidewall 317 opposite to the first warehouse sidewall. The first warehouse sidewall and the first extraction warehouse sidewall 11 have the same structure, the second sidewall 317 and the first sidewall 311 have the same structure, and the assembly method of the first warehouse sidewall and the second sidewall 317 is the same as the assembly method of the first extraction warehouse sidewall 11 and the first sidewall 311.

[0088] The document construction warehouse 2 has a first construction warehouse sidewall, which is the sidewall opposite to the transfer warehouse 31. The transfer warehouse 31 has a second sidewall 317, which is the sidewall opposite to the first construction warehouse sidewall. The first construction warehouse sidewall and the first extraction warehouse sidewall 11 have the same structure, and the second sidewall 317 and the first sidewall 311 have the same structure. Furthermore, the assembly method of the first construction warehouse sidewall and the second sidewall 317 is the same as the assembly method of the first extraction warehouse sidewall 11 and the first sidewall 311. This arrangement makes the structure of the document construction system 100 more reasonable, which helps to reduce the production and assembly difficulty of the document construction system 100, and also reduces the structural complexity of the document construction system 100, thereby reducing the production cost of the document construction system 100.

[0089] In some examples of this utility model, such as Figure 4 and Figure 5 As shown, at least one side wall of the extraction compartment 1 is provided with a first compartment door 12, which is used to open or close the extraction compartment 1, and / or at least one side wall of the construction compartment 2 is provided with a second compartment door 13, which is used to open or close the construction compartment 2.

[0090] In some embodiments of this application, at least one side wall of the extraction compartment 1 is provided with a first door 12, which is used to open or close the extraction compartment 1. In some embodiments of this application, at least one side wall of the construction compartment 2 is provided with a second door 13, which is used to open or close the construction compartment 2. In some embodiments of this application, at least one side wall of the extraction compartment 1 is provided with a first door 12, which is used to open or close the extraction compartment 1, and at least one side wall of the construction compartment 2 is provided with a second door 13, which is used to open or close the construction compartment 2. This application will describe an example where at least one side wall of the extraction compartment 1 is provided with a first door 12, which is used to open or close the extraction compartment 1, and at least one side wall of the construction compartment 2 is provided with a second door 13, which is used to open or close the construction compartment 2.

[0091] At least one side wall of the extraction chamber 1 is provided with a first door 12. For example, one side wall of the extraction chamber 1 may be provided with a first door 12, or both side walls of the extraction chamber 1 may be provided with a first door 12. The first door 12 is used to open or close the extraction chamber 1. When the first door 12 is open, it is convenient for staff to put the sample to be tested and the reagents and consumables required for the extraction equipment into the extraction chamber 1, so that the extraction equipment in the extraction chamber 1 can smoothly complete the extraction of the sample to be tested. When the first door 12 is closed, the extraction equipment in the extraction chamber can smoothly complete the extraction of the sample to be tested, reducing the risk of external environmental contamination of the sample to be tested during the extraction process.

[0092] At least one side wall of the cell preparation chamber 2 is provided with a second door 13. For example, one side wall of the cell preparation chamber 2 may be provided with a second door 13, or both side walls of the cell preparation chamber 2 may be provided with a second door 13. The second door 13 is used to open or close the cell preparation chamber 2. When the second door 13 is open, it is convenient for staff to put the reagents and consumables required for the cell preparation equipment into the cell preparation chamber 2 so that the cell preparation equipment in the cell preparation chamber 2 can complete the cell preparation work smoothly, or to take out the sample to be tested from the cell preparation chamber 2. When the second door 13 is closed, the cell preparation equipment in the cell preparation chamber 2 can complete the cell preparation work smoothly, reducing the risk of external environmental contamination of the sample to be tested during the cell preparation process.

[0093] In some examples of this utility model, such as Figure 4 and Figure 5 As shown, the first compartment door 12 and the second compartment door 13 are the same.

[0094] The first compartment door 12 and the second compartment door 13 are identical. This arrangement makes the arrangement of the first compartment door 12 and the second compartment door 13 reasonable. The identical structure of the first compartment door 12 and the second compartment door 13 helps to reduce the production and assembly difficulty of the document construction system 100. In addition, it can also reduce the structural complexity of the document construction system 100. Furthermore, by constructing the first compartment door 12 and the second compartment door 13 with the same structure, the number of molds for producing the first compartment door 12 and the second compartment door 13 during the production process can be reduced, thereby reducing the production cost of the document construction system 100.

[0095] In some examples of this utility model, such as Figure 4 and Figure 5 As shown, both the first compartment door 12 and the second compartment door 13 may include a door frame 121 and a movable door 122. The door frame 121 has a pick-up and put-out hole 123, and the movable door 122 is movably disposed on the door frame 121 to open or close the pick-up and put-out hole 123.

[0096] The door frame 121 has a pick-up and put-out hole 123, and the movable door 122 is movably disposed on the door frame 121. For example, the movable door 122 is slidably disposed on the door frame 121, or the movable door 122 is rotatably disposed on the door frame 121, so that the movable door 122 can open or close the pick-up and put-out hole 123. When the movable door 122 opens the pick-up and put-out hole 123, the staff can put the corresponding reagents and consumables into the corresponding extraction chamber 1 or the corresponding library construction chamber 2 through the pick-up and put-out hole 123. In addition, the staff can put the sample to be tested into the extraction chamber 1 through the pick-up and put-out hole 123 or take the sample to be tested out from the library construction chamber 2 through the pick-up and put-out hole 123, so that the library construction system 100 can test the sample to be tested multiple times.

[0097] In some examples of this utility model, such as Figure 4 and Figure 5As shown, the movable door 122 and the door frame 121 are arranged opposite to each other and are slidably disposed on the door frame 121 along the second direction, which is perpendicular to the first direction. A fourth sealing member 1211 is fixedly provided on the surface of the door frame 121 facing the movable door 122, and the fourth sealing member 1211 abuts against the movable door 122. A fifth sealing member 1221 is fixedly provided on the surface of the movable door 122 facing the door frame 121, and the fifth sealing member 1221 abuts against the door frame 121. When the movable door 122 closes the pick-up and put-out hole 123, the fourth sealing member 1211 and the fifth sealing member 1221 are arranged around the pick-up and put-out hole 123.

[0098] The movable door 122 and the door frame 121 are arranged opposite each other, and the movable door 122 is slidably mounted on the door frame 121 along the second direction. This arrangement makes the placement of the movable door 122 reasonable, reducing the risk of large-scale exchange between the air inside the extraction chamber 1 and the external ambient air, and between the air inside the preparation chamber and the external ambient air, during the opening or closing of the movable door 122, thereby further reducing the risk of contamination of the sample to be tested. The second direction is perpendicular to the first direction, that is, the X direction is perpendicular to the Y direction.

[0099] A fourth sealing element 1211 is fixedly provided on the door frame 121 facing the movable door 122, and the fourth sealing element 1211 abuts against the movable door 122. A fifth sealing element 1221 is fixedly provided on the movable door 122 facing the door frame 121, and the fifth sealing element 1221 abuts against the door frame 121. When the movable door 122 closes the access hole 123, the fourth sealing element 1211 and the fifth sealing element 1221 are arranged around the access hole 123. For example, the fourth sealing element 1211 can be constructed as an "n" type seal, and the fifth sealing element 1221 can be constructed as a "u" type seal, or the fourth sealing element 1211 can be constructed as a "u" type seal, and the fifth sealing element 1221 can be constructed as an "n" type seal. The fourth seal 1211 and the fifth seal 1221 can be fixed to the corresponding door frame 121 and movable door 122 by means of bolts, adhesives, etc. When the movable door 122 closes the access hole 123, the fourth seal 1211 and the fifth seal 1221 are arranged opposite to each other along the second direction, and the fourth seal 1211 and the fifth seal 1221 abut against each other, so that the fourth seal 1211 and the fifth seal 1221 together form a relatively sealed closed loop, thereby achieving the effect of relatively sealing the internal and external environments of the extraction chamber 1 and the construction chamber 2.

[0100] In some examples of this utility model, such as Figure 4 and Figure 5As shown, the fourth sealing element 1211 may include: a sixth sub-sealing strip 1212 and two seventh sub-sealing strips 1213. Along the second direction, the sixth sub-sealing strip 1212 is located at the upper edge of the door frame 121, and the two seventh sub-sealing strips 1213 extend along the second direction and are respectively located at the two side edges of the door frame 121. The sixth sub-sealing strip 1212 is connected between the two fifth sub-sealing strips.

[0101] The fifth sealing element 1221 may include: an eighth sub-sealing strip 1222 and two ninth sub-sealing strips 1223. Along the second direction, the eighth sub-sealing strip 1222 is located at the lower edge of the movable door 122, and the two ninth sub-sealing strips 1223 extend along the second direction and are respectively located at the two side edges of the movable door 122. The eighth sub-sealing strip 1222 is connected between the two ninth sub-sealing strips 1223. The seventh sub-sealing strip 1213 and the corresponding ninth sub-sealing strip 1223 are arranged along the width direction of the pick-up and drop hole 123.

[0102] In this configuration, along the second direction, the sixth sub-sealing strip 1212 is located at the upper edge of the door frame 121, and two seventh sub-sealing strips 1213 extend along the second direction, with each of the seven seventh sub-sealing strips 1213 located at one of the two side edges of the door frame 121. The sixth sub-sealing strip 1212 is connected between the two fifth sub-sealing strips, so that the fourth sealing element 1211 forms a continuous sealing structure. Along the second direction, the eighth sub-sealing strip 1222 is located at the lower edge of the movable door 122, and two ninth sub-sealing strips 1223 extend along the second direction, with each of the nine ninth sub-sealing strips 1223 located at one of the two side edges of the movable door 122. The eighth sub-sealing strip 1222 is connected between the two ninth sub-sealing strips 1223, so that the fifth sealing element 1221 forms a continuous sealing structure.

[0103] The seventh sub-seal strip 1213 and the corresponding ninth sub-seal strip 1223 are arranged along the width direction of the take-up and put-out hole 123. The seventh sub-seal strip 1213 and the corresponding ninth sub-seal strip 1223 can be abutted together or have a certain gap. The gap is small (less than or equal to 2mm). For example, the gap between the seventh sub-seal strip 1213 and the corresponding ninth sub-seal strip 1223 is about 2mm. This is to minimize the air output of the extraction chamber 1 and the storage chamber 2 when the movable door 122 closes the take-up and put-out hole 123, while achieving the effect of smooth sliding of the movable door 122.

[0104] The eighth sub-seal strip 1222 is in close contact with the platform of extraction chamber 1 or construction chamber 2, achieving relative sealing of the internal and external environments of extraction chamber 1 or construction chamber 2. At the end of the experiment, when the movable door 122 is opened, the fifth seal 1221 moves with the movable door 122, and the ninth sub-seal strip 1223 eventually aligns with the seventh sub-seal strip 1213, and the eighth sub-seal strip 1222 eventually aligns with the sixth sub-seal strip 1212. This improves the sliding efficiency of the movable door 122 and avoids the accelerated aging of the sealant strips due to exposure to air.

[0105] This design breaks through the limitations of traditional logical deduction. Early use of solid rubber strips resulted in high pushing resistance and inconvenient installation for the sliding door 122. Hollow rubber strips were later used, offering lower frictional resistance and allowing for screw fixation, resulting in excellent installation. The layout of the fifth seal 1221 and the sixth seal 3233 was also optimized from the initial double-circle design to an "n" and "u" shape combination. This not only facilitates the pushing of the sliding door 122 and reduces material costs, but also reduces wear and aging of the fifth seal 1221 and the sixth seal 3233 during opening and closing, extending their service life, reducing maintenance costs, and achieving efficient sealing and low-friction sliding. Combined with optimized airflow control, the system can maintain stable pressure differential and unidirectional airflow, while reducing wear and maintenance costs of the fifth seal 1221 and the sixth seal 3233, meeting the sealing and unidirectional airflow requirements of the library construction system 100 in a simple and low-cost manner.

[0106] In some examples of this utility model, such as Figure 1 As shown, the air pressure inside extraction chamber 1 is greater than the air pressure inside construction chamber 2.

[0107] In this system, the air pressure in extraction chamber 1 is greater than that in library construction chamber 2. For example, the air pressure in library construction chamber 2 is 5 Pa and the air pressure in extraction chamber 1 is 10 Pa, or the air pressure in library construction chamber 2 is 6 Pa and the air pressure in extraction chamber 1 is 12 Pa. By ensuring that the air pressure in extraction chamber 1 is greater than that in library construction chamber 2, the airflow within the library construction system 100 can always be transferred from extraction chamber 1 to library construction chamber 2 during the movement of the sample to be tested. The airflow direction remains unidirectional, effectively reducing the risk of cross-contamination between the gases in extraction chamber 1 and library construction chamber 2.

[0108] In some examples of this utility model, such as Figure 1 As shown, the air pressure inside the transfer chamber 31 is less than the air pressure inside the extraction chamber 1 but greater than the air pressure inside the storage chamber 2.

[0109] The air pressure in the transfer chamber 31 is lower than that in the extraction chamber 1 but higher than that in the library construction chamber 2. This setting allows for a reasonable air pressure distribution in the extraction chamber 1, transfer chamber 31, and library construction chamber 2. During the movement of the sample to be tested, the airflow within the library construction system 100 is always transferred from the extraction chamber 1 to the transfer chamber 31 and then from the transfer chamber 31 to the library construction chamber 2. This helps maintain a single airflow direction and effectively reduces the risk of cross-contamination between the gases in the extraction chamber 1 and the library construction chamber 2.

[0110] Specifically, the transfer chamber 31 has a second sealing door. The library construction system 100 is equipped with a detection program. When the sample to be tested needs to be transferred from the extraction chamber 1 through the transfer chamber 31 to the library construction chamber 2, the air pressure in the library construction chamber 2 is 5 Pa, and the air pressure in the extraction chamber 1 is 10 Pa. The detection program first controls the motor of the first sealing door 313 to open the first sealing door 313.

[0111] Because the sealing effect of the rubber strips between the transfer chamber 31 and the extraction chamber 1 is good, the air pressure inside the transfer chamber 31 is the same as the external ambient air pressure, while the air pressure in the extraction chamber 1 is about 10 Pa higher than that in the transfer chamber 31. Furthermore, since the volume of the extraction chamber 1 is much larger than that of the transfer chamber 31 (the volume of the extraction chamber 1 is about 17 times that of the transfer chamber 31), after the first sealing door 313 is opened, the air pressure inside the extraction chamber 1 and the transfer chamber 31 will quickly exchange and reach equilibrium, and eventually the overall pressure will drop to slightly below 10 Pa (at this time, the air pressure inside the storage chamber 2 is still about 5 Pa).

[0112] Meanwhile, the detection program controls the conveying mechanism 32 to transfer the sample to be tested into the transfer chamber 31, and then closes the first sealing door 313. Since the contact surface between the first sealing door 313 and the conveying mechanism 32 is sealed by the first sealing element 316, after the first sealing door 313 is closed, the air in the transfer chamber 31 almost no longer exchanges with the air in the extraction chamber 1. At this time, the extraction chamber 1, the transfer chamber 31 and the storage chamber 2 all form relatively sealed independent spaces.

[0113] Subsequently, the detection program controls the second sealing door motor to open the second sealing door. Based on the same principle, the air pressure in the transfer chamber 31 and the preparation chamber 2 will rapidly exchange and reach equilibrium, eventually rising to slightly above 5 Pa (at this point, the air pressure in the extraction chamber 1 returns to approximately 10 Pa). Simultaneously, the air in the transfer chamber 31 and the preparation chamber 2 will not flow into the extraction chamber 1. The software-controlled conveying mechanism 32 transfers the sample to be tested from the transfer chamber 31 to the preparation chamber 2, and then closes the second sealing door.

[0114] At this point, the air exchange between the transfer chamber 31 and the construction chamber 2 ceases, and the extraction chamber 1, transfer chamber 31, and construction chamber 2 form a relatively sealed physical space. The sample to be tested is successfully transferred from the extraction chamber 1 to the construction chamber 2. Throughout the process, the airflow always flows from the extraction chamber 1 through the transfer chamber 31 to the construction chamber 2, with a single flow direction, effectively reducing the risk of cross-contamination of gases within the extraction chamber 1 and the construction chamber 2.

[0115] In some examples of this utility model, such as Figure 1 As shown, the extraction chamber 1 has a first gas driving component 13 and a first filter element 14. The first gas driving component 13 is used to drive gas into and out of the extraction chamber 1 to regulate the gas pressure inside the extraction chamber 1. The first filter element 14 is used to filter the gas flowing into the extraction chamber 1.

[0116] The first filter element 14 may be, but is not limited to, a filter cotton. The first gas driving component 13 is used to drive gas into and out of the extraction chamber 1 to adjust the gas pressure in the extraction chamber 1, so that the gas pressure in the extraction chamber 1 is always higher than the gas pressure in the construction chamber 2, thereby achieving the effect of unidirectional airflow and reducing the risk of cross-contamination between the gases in the extraction chamber 1 and the construction chamber 2.

[0117] The first gas driving assembly 13 may include multiple first sub-gas driving elements 131. For example, the first gas driving assembly 13 may include two, three or more first sub-gas driving elements 131. The first sub-gas driving elements 131 may be constructed as fans, air pumps, etc. In this application, two first sub-gas driving elements 131 are used as an example for description. One first sub-gas driving element 131 is used to drive gas into the extraction chamber 1, and the other first sub-gas driving element 131 is used to drive gas out of the extraction chamber 1. By adjusting the driving speed of the two first sub-gas driving elements 131, the gas pressure in the extraction chamber 1 can be adjusted.

[0118] Furthermore, the first filter element 14 is disposed at the first sub-gas drive element 131 for driving gas into the extraction chamber 1, so that the gas can enter the extraction chamber 1 after being filtered by the first filter element 14, thereby reducing the risk of contamination of the sample to be tested in the extraction chamber 1.

[0119] In some examples of this utility model, such as Figure 1 As shown, the gas-construction chamber 2 has a second gas-driven assembly 21 and a second filter element 22. The second gas-driven assembly 21 is used to drive gas into and out of the gas-construction chamber 2 to regulate the gas pressure inside the gas-construction chamber 2. The second filter element 22 is used to filter the gas flowing into the gas-construction chamber 2.

[0120] The second filter element 22 may be, but is not limited to, a filter cotton. The second gas drive component 21 is used to drive gas into and out of the building chamber 2 to regulate the gas pressure inside the building chamber 2, so that the gas pressure inside the building chamber 2 is always lower than the gas pressure inside the extraction chamber 1, thereby achieving the effect of unidirectional airflow and reducing the risk of cross-contamination between the gases in the extraction chamber 1 and the building chamber 2.

[0121] The second gas driving assembly 21 may include multiple second sub-gas driving elements 211. For example, the second gas driving assembly 21 may include two, three or more second sub-gas driving elements 211. The second sub-gas driving elements 211 may be constructed as fans, air pumps, etc. This application takes two second sub-gas driving elements 211 as an example for description. One second sub-gas driving element 211 is used to drive gas into the building chamber 2, and the other second sub-gas driving element 211 is used to drive gas out of the building chamber 2. By adjusting the driving speed of the two second sub-gas driving elements 211, the gas pressure in the building chamber 2 can be adjusted.

[0122] Furthermore, the second filter element 22 is disposed at the second sub-gas drive element 211 used to drive the gas into the construction chamber 2, so that the gas can be filtered by the second filter element 22 before entering the construction chamber 2, thereby reducing the risk of contamination of the sample to be tested in the construction chamber 2.

[0123] In some examples of this utility model, such as Figure 6 As shown, the conveying mechanism 32 includes a drive mechanism 322, a guide rail 323, and a placement platform 324. The guide rail 323 passes through the transfer chamber 31 along a first direction, and both ends of the guide rail 323 extend into the extraction chamber 1 and the storage chamber 2, respectively. The placement platform 324 is located outside the guide rail 323 and is used to place the sample to be tested. At least a portion of the drive mechanism 322 is located inside the guide rail 323 and is connected to the placement platform 324 in a transmission manner. The drive mechanism 322 is used to drive the placement platform 324 to reciprocate relative to the guide rail 323 along the first direction, so that the placement platform 324 moves into one of the extraction chamber 1, the storage chamber 2, and the transfer chamber 31.

[0124] The guide rail 323 extends along a first direction and passes through the transfer chamber 31. Both ends of the guide rail 323 extend into the extraction chamber 1 and the storage chamber 2, respectively. The placement table 324 is located outside the guide rail 323 to facilitate the placement of the sample to be tested by the operator. At least a portion of the drive mechanism 322 is located within the guide rail 323; for example, a portion of the drive mechanism 322 may be located within the guide rail 323, or the entire structure of the drive mechanism 322 may be located within the guide rail 323. The drive mechanism 322 is connected to the placement table 324 via a transmission connection, allowing the drive mechanism 322 to drive the placement table 324 to reciprocate relative to the guide rail 323 along the first direction. This facilitates the smooth movement of the placement table 324 into one of the extraction chamber 1, the storage chamber 2, and the transfer chamber 31, thereby sequentially extracting and storing the sample to be tested within the placement table 324.

[0125] In some examples of this utility model, such as Figure 6 and Figure 7 As shown, the guide rail 323 has a guide rail wall 3231 facing the placement platform 324. The guide rail wall 3231 has a clearance hole extending along a first direction. The drive mechanism 322 includes a drive rod 3221, which passes through the clearance hole. The clearance hole is provided with a sixth sealing member 3233, which is used to seal the clearance hole. The sixth sealing member 3233 includes a first sealing strip 3234 and a second sealing strip 3235. The first sealing strip 3234 and the second sealing strip 3235 both extend along the first direction and are located on both sides of the drive rod 3221. The first sealing strip 3234 and the second sealing strip 3235 both abut against the drive rod 3221 for sealing.

[0126] The guide rail 323 has a guide rail wall 3231, which forms a side wall of the guide rail 323 facing the placement platform 324. The guide rail wall 3231 has a clearance hole extending in a first direction, which can allow the drive mechanism 322 to pass through. Further, the drive mechanism 322 includes a drive rod 3221, which passes through the clearance hole so that the drive rod 3221 can be fixedly connected to the placement platform 324, thereby achieving the effect of the drive mechanism 322 driving the placement platform 324 to move via the drive rod 3221. The clearance hole is provided with a sixth seal 3233, which may be, but is not limited to, made of rubber, silicone, foam, etc. The sixth seal 3233 is used to seal the clearance hole, reducing the risk of external air entering the extraction chamber 1, the construction chamber 2, or the transfer chamber 31 through the clearance hole.

[0127] The sixth sealing element 3233 includes a first sealing strip 3234 and a second sealing strip 3235. Both the first sealing strip 3234 and the second sealing strip 3235 extend along a first direction and along a third direction. The first sealing strip 3234 and the second sealing strip 3235 are located on both sides of the drive rod 3221, and both the first sealing strip 3234 and the second sealing strip 3235 abut against the drive rod 3221 to seal, so as to reduce the risk of external air entering the extraction chamber 1, the construction chamber 2 or the transfer chamber 31 through the gap between the drive rod 3221 and the guide rail wall 3231 when the drive rod 3221 moves along the first direction.

[0128] like Figure 7 As shown, the guide rail 323 may also be fixed with two pressure strips 3232. The two pressure strips 3232 are respectively fixed to the ends of the first sealing strip 3234 and the second sealing strip 3235 that are away from each other. The first sealing strip 3234 and the second sealing strip 3235 are made of elastic materials, such as rubber or silicone. By setting the pressure strips 3232, the first sealing strip 3234 and the second sealing strip 3235 can be interference-fitted between the corresponding pressure strip and the drive rod 3221, which not only improves the sealing effect of the first sealing strip 3234 and the second sealing strip 3235, but also further improves the sealing effect of the library construction system 100, and ensures that the drive rod 3221 slides smoothly.

[0129] The first sealing strip 3234 and the second sealing strip 3235 do not affect the back-and-forth movement of the drive rod 3221, and can maintain the relative stability of the height of the placement platform 324, avoiding the swaying of the guide rail 323 due to excessive friction, which would affect the accuracy of the height of the placement platform 324. This design ensures the smoothness of the drive mechanism 322 during operation, and at the same time, it precisely fills the gap in the transmission direction of the guide rail 323, preventing air exchange between the extraction chamber 1, the storage chamber 2, or the transfer chamber 31 and the outside. In addition, the sixth sealing element 3233 in the guide rail 323 is in close contact with the rubber strip at the bottom of the first sealing door 313 and the second sealing door of the transfer chamber 31, which can effectively prevent airflow between the extraction chamber 1, the storage chamber 2, or the transfer chamber 31.

[0130] The guide rail 323 located within the extraction chamber 1 and the construction chamber 2 also serves to support the placement stage 324. The guide rail 323 may have a fixed base 3236. By adjusting the height of the fixed base 3236 at the bottom of the guide rail 323, the placement stage 324 can remain horizontal at both ends of the guide rail 323 along the first direction (i.e., within the extraction chamber 1 or the construction chamber 2), ensuring that the automatic pipette within the chamber can perform precise pipetting actions. This design meets the requirement for automatic transfer of the placement stage 324 between the extraction chamber 1 and the construction chamber 2, realizing the function of the placement stage 324 as a reagent exchange carrier between the extraction chamber 1 and the construction chamber 2.

[0131] As some embodiments of this application, the drive mechanism 322 further includes a drive motor 3222, a transmission belt 3223, and a connector 3224. The drive motor 3222 may be, but is not limited to, a stepper motor, a linear motor, etc. The drive motor 3222 has an output end, which is connected to the transmission belt 3223 so that the drive motor 3222 can drive the transmission belt 3223 to rotate. The connector 3224 and the transmission belt 3223 may be fixedly connected by bolts, snap-fits, etc., and the connector 3224 is fixedly connected to the drive rod 3221. The drive motor 3222 drives the transmission belt 3223, thereby driving the connector 3224 to move. The movement of the connector 3224 can drive the drive rod 3221, thereby driving the placement platform 324 to reciprocate along the first direction relative to the guide rail 323. This facilitates the smooth movement of the placement platform 324 into one of the extraction chamber 1, the storage chamber 2, and the transfer chamber 31, so that the sample to be tested in the placement platform 324 can be extracted and stored sequentially.

[0132] It should be noted that during the sample testing process, the placement stage 324 only needs to be transferred from the extraction chamber 1 to the preparation chamber 2. Only after the testing operation is completed will the placement stage 324 return to the extraction chamber 1 under the control of the testing program.

[0133] This application innovatively integrates the extraction chamber 1 and the preparation chamber 2 into the same IVD (in vitro diagnostic) device. Through coordinated optimization of software and hardware, efficient and contamination-free transfer of the placement stage 324 is achieved. The extraction chamber 1 and the preparation chamber 2 feature a single-direction, independently sealed airflow system. The core component of the contamination-free material exchange system for the extraction chamber 1 and the preparation chamber 2 is the conveying mechanism 32. This module not only transports the sample but also, through overall software control, coordinates the movement of the motor and drive belt 3223 of the placement stage 324, as well as the independent opening and closing of the first and second sealing doors, when connecting the extraction chamber 1 and the preparation chamber 2. During sample transport, this module maintains relative stability of the air pressure within each chamber and a stable pressure difference between the extraction chamber 1 and the preparation chamber 2, thereby ensuring unidirectional airflow and minimizing the risk of cross-contamination.

[0134] As some embodiments of this application, aerosols are easily generated during gene detection library construction PCR (polymerase chain reaction), causing cross-contamination. This method uses mineral oil for liquid surface sealing during library construction PCR to avoid aerosol generation. Furthermore, environmental contamination is regularly monitored in extraction chamber 1 and library construction chamber 2 to ensure that contamination levels within the chambers are below the positive detection threshold. Throughout the entire process of using the library construction system 100 to detect gene products, a product quality control system is used for monitoring to determine whether cross-contamination occurs in the same batch of test results.

[0135] Aerosols are easily generated during the library construction PCR process, so an oil seal technology is adopted. The specific steps of applying the oil seal technology in this system are as follows: Step 1: The library construction system 100 is started, and the pressure difference between the extraction chamber 1 and the library construction chamber 2 is maintained at about 5 Pa, and the air flow direction is single. Step 2: When detecting pathogenic genes, the test sample, product quality control, and blank are detected simultaneously, and the whole process is monitored. Step 3: After the sample is extracted in the extraction chamber 1, it is transferred to the library construction chamber 2 through the transfer chamber 31 whose air flow direction between chambers can be controlled structurally. Step 4: During the library construction PCR, mineral oil is used for sealing to avoid cross-contamination of aerosols. Step 5: After sequencing based on the next-generation sequencing platform, the integrated machine supporting the library construction system 100 performs quality control on the raw sequencing data, removes the host from the data, and obtains the待比对结果 (to-be-compared result). Step 6: The integrated machine supporting the library construction system 100 compares the obtained to-be-compared result with the comparison database pre-established in the system to determine the detected main pathogenic organisms.

[0136] The following are two methods for regular pollution detection: Surface wiping sampling method: After the swab samples multiple points on the inner surface of the library construction system 100, routine detection is carried out (Steps 1 to 6).

[0137] Air sedimentation sampling method: After the sampling tubes filled with normal saline are placed at multiple points inside the library construction system 100 for 24 hours respectively, routine detection is carried out (Steps 1 to 6).

[0138] As some embodiments of this application, the library construction system 100 controls the operation of the device through program settings and can automatically complete the whole-process experiment and analysis from the sample to the report. The construction of a conventional NGS laboratory (high-throughput sequencing laboratory) needs to pay attention to the wind direction to form convection to reduce cross-contamination, and the integrated all-in-one device for the whole process can also meet this requirement. By separating the two experimental chambers of the extraction chamber 1 and the library construction chamber 2, the periphery of the extraction chamber 1 and the library construction chamber 2 is designed with strip seals, and HEPA (high-efficiency air filtration) systems, filter cotton, and small fans are respectively equipped to ensure the relative stability of the air pressure difference between the extraction chamber 1 and the library construction chamber 2. When the placement table 324 is conveyed by the conveying mechanism 32, by controlling the opening sequence of the first sealing door 313 and the second sealing door, the air flow is made to flow from the extraction chamber 1 to the library construction chamber 2 singly.

[0139] Description of pollution control in the experimental process: The oil seal technology is to add mineral oil liquid seal to all the links reacting in the PCR instrument to avoid cross-contamination of aerosols. In the extraction chamber 1 and the library construction chamber 2, for the regular environmental monitoring process of the equipment, after obtaining environmental samples through the surface wiping sampling and air sedimentation sampling methods, the routine sample detection process is used to monitor the pollution situation of the environment.

[0140] ① Oil-sealing technology: In the fully automated process, mineral oil is added to all reaction stages of the PCR instrument for liquid sealing to avoid aerosol contamination and prevent the reaction solution from evaporating at high temperatures, thereby improving the stability of the PCR reaction. Two control groups were designed: high-positive enterprise reference samples and negative samples were arranged alternately. In one group, mineral oil was added to the process for detection, and the results showed that no positive pathogens were detected in any of the negative sample wells; in the other group, no mineral oil was added, and positive pathogens were detected in some of the negative sample wells. This indicates that in the fully automated process, oil-sealing technology can effectively prevent false positive results caused by aerosol contamination during detection. In the fully automated process of gene detection, the innovative addition of mineral oil to the reaction stages of the PCR instrument for liquid sealing, combined with the fully automated equipment system, effectively avoids aerosol contamination between test samples.

[0141] ② Regular environmental monitoring process for the equipment: An environmental monitoring experiment was designed to periodically obtain environmental samples in extraction chamber 1 and preparation chamber 2 using surface wiping sampling and air sedimentation sampling methods. These samples were then processed according to a fully automated clinical sample process to monitor the pollution level of the usage environment. An effective regular environmental monitoring process was innovatively introduced into the entire fully automated equipment system to ensure the reliability of the test results.

[0142] This invention effectively addresses the stringent requirements of gene testing on the experimental environment and personnel by implementing a comprehensive solution. In a standard laboratory environment of approximately 8 square meters, a single person can complete the entire automated gene testing process and generate a report. The library construction system is cost-effective and controllable, and its design meets the basic requirements of a standard molecular PCR laboratory. By physically separating the extraction and library construction stages and connecting them via a transfer device 3, and using a sealed adhesive strip design around the equipment, a negative pressure difference of 5 Pa can be achieved between the extraction chamber 1 and the library construction chamber 2, effectively preventing cross-contamination. During the experiment, an innovative "oil-sealing technology" is used in necessary steps, and environmental pollution assessments are conducted regularly to ensure the accuracy of the test results.

[0143] Example of verifying the system's anti-pollution effect:

[0144] In oil sealing technology, Solarbio M8040 mineral oil (paraffin oil) is used. It is a colorless, transparent liquid with a density of 0.84 g / ml (25°C). It is stable to light, heat, and acid, insoluble in water and ethanol, but soluble in volatile oils and most non-volatile oils. Its melting point is -24°C (literature value), and its boiling point is 300°C (literature value). Storage conditions include room temperature and sealing. This product is widely used as a matrix ingredient in cosmetics and in PCR reactions to cover samples and prevent evaporation of the reaction mixture during the reaction.

[0145] In the library construction system 100, the mineral oil sealing method has significant advantages in detecting pathogenic products, effectively avoiding cross-contamination between samples in the same batch within the equipment, and playing a role in isolation and stabilization:

[0146] Isolation: Covering the reaction system forms an oil film, blocking aerosol diffusion and preventing cross-contamination of samples.

[0147] Stability: Protects the reaction system from external temperature and humidity, improves the efficiency and specificity of the PCR reaction, and ensures accurate and reliable test results.

[0148] Compatibility: Compatible with automated equipment and does not affect subsequent testing processes, such as sequencing and data analysis.

[0149] A. Evaluation of the aerosol control effect of "oil seal technology":

[0150] (1) Comparison of oil-sealed and non-oil-sealed experimental designs for automated gene detection: DNA was extracted from negative and high-positive enterprise reference samples, negative and positive quality control samples from the kit, and blank control samples. Library preparation was performed using a fully automated gene detection device, following the detection workflow of BGI Genomics' PTseq respiratory pathogen targeted high-throughput gene detection kit. Sequencing was then performed on a DNB sequencing device using the accompanying kit. Finally, the results were analyzed using a gene analysis integrated machine, and a test report was generated. Two rounds of testing were conducted: the first round of control experiment did not use oil sealing, and high-positive and negative reference samples were arranged alternately in a checkerboard pattern for the entire detection process; the second round of experiment used oil sealing in the reaction stage of the PCR instrument, with the remaining design consistent with the first round. Standard equipment cleaning procedures were performed before and after each round of experiment. The comparative analysis results are shown in Table 1 below.

[0151] Table 1. Comparison of detection results for high-throughput automated gene detection of pathogenic microorganisms with and without oil sealing:

[0152]

[0153] Note: The original detection results for the high-positive reference material were Streptococcus pneumoniae, Acinetobacter baumannii, Staphylococcus aureus, Candida albicans, and human gamma herpesvirus type 4 (EBV). The concentrations of each pathogen in the positive reference material exceeded 95% of the corresponding pathogen concentrations in patient samples from the intended user population. The original detection results for the positive control were Schizophrenia marina and MS2; the original detection results for the negative reference material (HeLa cell matrix) and the negative control were negative.

[0154] (2) Results of automated gene detection with and without oil sealing: Table 1 shows that in the unsealed case, two negative reference wells of the high-throughput automated gene detection for pathogenic microorganisms tested positive for human gamma herpesvirus type 4 (EBV). However, when mineral oil was added during the reaction in the PCR instrument, no bacteria / viruses were detected in the negative reference wells. Contamination rate calculation: Without oil sealing, a total of 3 reads of human gamma herpesvirus type 4 (EBV) were detected in the negative reference wells, and 2,622,493 reads of human gamma herpesvirus type 4 (EBV) were detected in the positive reference wells, with a contamination rate of 1 / 874,164; With oil sealing, 0 reads of human gamma herpesvirus type 4 (EBV) were detected in the negative reference wells, and 11,707,887 reads of human gamma herpesvirus type 4 (EBV) were detected in the positive reference wells, with a contamination rate of less than 1 / 11,707,887. The use of mineral oil seals in this fully automated equipment effectively avoids cross-contamination between samples from the same batch within the equipment and ensures that the detected sample results are completely consistent with expectations.

[0155] (3) Experimental Design Comparing Fully Automated and Manual Testing: DNA was extracted from negative and high-positive enterprise reference samples, negative and positive quality control samples from the kit, and blank control samples. Library preparation was performed using fully automated gene detection equipment, following the detection workflow of the DaGene PTseq Respiratory Infection Pathogen Targeted High-Throughput Gene Detection Kit. Sequencing was then performed on the DNB sequencing equipment using the accompanying kit. Finally, the results were analyzed using a gene analysis machine, and a test report was generated. Two rounds of testing were conducted: the first round of control experiments used oil-sealing technology, arranging high-positive and negative reference samples alternately in a checkerboard pattern for fully automated testing; the second round of experiments was completed in a standard high-throughput laboratory, with each step of the testing performed by certified laboratory personnel in their respective zones (i.e., personnel performed extraction and library construction on the extraction equipment in the sample extraction laboratory and the library construction equipment in the library construction laboratory, respectively, and finally used the sequencer and gene analysis machine to complete the sequencing and analysis). Standard equipment cleaning procedures were performed before and after each round of experiments. The comparative analysis results are shown in Table 2 below.

[0156] Table 2 Comparison of Detection Results of High-Throughput Fully Automated Gene Detection and Manual Detection of Pathogenic Microorganisms

[0157]

[0158] Note: The original detection results for the high-positive reference material were Streptococcus pneumoniae, Acinetobacter baumannii, Staphylococcus aureus, Candida albicans, and human gamma herpesvirus type 4 (EBV). The concentrations of each pathogen in the positive reference material exceeded 95% of the corresponding pathogen concentrations in patient samples from the intended user population. The original detection results for the positive control were Schizophrenia marina and MS2; the original detection results for the negative reference material (HeLa cell matrix) and the negative control were negative.

[0159] (4) Comparison of results between fully automated and manual testing: Table 2 shows that in the high-throughput automated gene detection of pathogenic microorganisms (using oil-sealing technology), no out-of-line bacteria / viruses were detected in any of the negative reference wells. Contamination rate calculation: 0 reads of human gamma herpesvirus type 4 (EBV) were detected in the negative reference wells, and 11,707,887 reads of human gamma herpesvirus type 4 (EBV) were detected in the positive reference wells, with a contamination rate of less than 1 / 11,707,887. In contrast, manual testing showed 1 read of human gamma herpesvirus type 4 (EBV) detected in the negative reference wells, and 2,319,337 reads of human gamma herpesvirus type 4 (EBV) detected in the positive reference wells, with a contamination rate of 1 / 2,319,337. This indicates that the overall performance of the automated system (including oil-sealing technology) is superior to that of manual testing under standard laboratory conditions.

[0160] B. Regular environmental monitoring and evaluation:

[0161] Standard cleaning procedure for the equipment: After use, the cover and base of the PCR instrument should be wiped with nucleic acid remover, then wiped with ultrapure water, and finally wiped with 75% ethanol. Select the late cleaning option in the software and irradiate with ultraviolet light for 15-30 minutes to complete the late cleaning. Before using the equipment, wipe each surface with ultrapure water, then wipe with 75% ethanol. Select the early cleaning option in the software and irradiate with ultraviolet light for 15-30 minutes.

[0162] Regular environmental monitoring: Surface wiping and air deposition sampling monitoring are conducted monthly, and every two weeks when the equipment is used frequently.

[0163] Surface wiping sampling: Sample processing & nucleic acid extraction area: Moisten a sterile cotton swab in an ultrapure water sampling tube and gently wipe the magnetic head, wells, and workstation surface (randomly select 6 magnetic heads and wells, and mark an "S" on the workstation surface), covering as much of the main operating area as possible. After sampling, place the cotton swab in the sampling tube at a certain angle, break the cotton swab, and close the cap for testing (number A1); Library amplification / product detection area: Moisten a sterile cotton swab in an ultrapure water sampling tube and gently wipe the workstation robotic arm, pipette tip, and workstation surface (randomly select 6 workstation surfaces and mark an "S" on them), randomly select 6 wells in the PCR module, the area under the PCR cover plate and the area under the reagent cover plate, and after sampling, place the cotton swab in the sampling tube at a certain angle, break the cotton swab, and close the cap for testing (number A2).

[0164] Air sedimentation sampling method: Place one 2ml tube (numbered B1 and B2) containing 1mL of ultrapure water in each of the sample processing & nucleic acid extraction area and the library amplification / product detection area. Open the tube cap and place it on the table in the corresponding area of ​​the running equipment for 8 hours, or place it on the table in the corresponding area of ​​the equipment for 24 hours before the experiment (the equipment must be turned on to ensure the airflow direction).

[0165] Detection method: The samples (numbered A1, A2, B1, B2), one negative control, one weak positive control, and one blank well collected by the above sampling method were subjected to fully automated detection.

[0166] Environmental monitoring results: Samples A1, A2, B1, B2, negative controls, and blank wells showed no abnormalities. The weakly positive control showed *Schizosaccharomyces cerevisiae*, MS2, consistent with expectations. The environmental monitoring results indicate that environmental pollution within the warehouse during the operation of the fully automated equipment is controllable.

[0167] In conclusion, the use of mineral oil sealing in this fully automated equipment for detecting pathogens can significantly reduce the risk of cross-contamination. Furthermore, regular contamination monitoring throughout the entire equipment operation process ensures the controllability of environmental pollution and guarantees the accuracy of test results, demonstrating significant application value and promising prospects for wider adoption.

[0168] In the description of this utility model, it should be understood that the terms "center", "longitudinal", "transverse", "length", "width", "thickness", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", "clockwise", "counterclockwise", "axial", "radial", "circumferential", etc., indicating the orientation or positional relationship are based on the orientation or positional relationship shown in the accompanying drawings, and are only for the convenience of describing this utility model and simplifying the description, and are not intended to indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation, and therefore should not be construed as a limitation of this utility model.

[0169] In the description of this utility model, "first feature" and "second feature" may include one or more of the features.

[0170] In the description of this utility model, "multiple" means two or more.

[0171] In the description of this utility model, the first feature being "above" or "below" the second feature may include the first and second features being in direct contact, or it may include the first and second features not being in direct contact but being in contact through another feature between them.

[0172] In the description of this utility model, the terms "above", "over" and "on top" for the first feature and the second feature include the first feature being directly above or diagonally above the second feature, or simply indicate that the first feature is at a higher horizontal level than the second feature.

[0173] In the description of this specification, the references to terms such as "one embodiment," "some embodiments," "illustrative embodiment," "example," "specific example," or "some examples," etc., indicate that a specific feature, structure, material, or characteristic described in connection with that embodiment or example is included in at least one embodiment or example of the present invention. In this specification, the illustrative expressions of the above terms do not necessarily refer to the same embodiment or example. Furthermore, the specific features, structures, materials, or characteristics described may be combined in any suitable manner in one or more embodiments or examples.

[0174] Although embodiments of the present invention have been shown and described, those skilled in the art will understand that various changes, modifications, substitutions and alterations can be made to these embodiments without departing from the principles and spirit of the present invention, the scope of which is defined by the claims and their equivalents.

Claims

1. A library construction system for gene detection, characterized in that, include: An extraction chamber, wherein an extraction device is installed in the extraction chamber, and the extraction device is used to extract the sample to be tested; The extraction chamber and the library construction chamber are equipped with library construction equipment, which is used to convert the extracted sample to be tested into a standardized library that can be identified. The extraction chamber and the library construction chamber are arranged along a first direction and are spaced apart. A transfer device includes a transfer chamber and a conveying mechanism. The transfer chamber is disposed between the extraction chamber and the silo construction chamber. The conveying mechanism passes through the transfer chamber along a first direction, with one end of the conveying mechanism extending into the extraction chamber and the other end extending into the silo construction chamber. Both the extraction chamber and the silo construction chamber can be selectively connected to the transfer chamber, so that the conveying mechanism transports the sample to be tested in the extraction chamber to the silo construction chamber through the transfer chamber.

2. The library construction system for gene detection according to claim 1, characterized in that, The extraction chamber has a first notch on its sidewall opposite to the transfer chamber, and the transfer chamber has a second notch on its sidewall opposite to the first extraction chamber. The first notch and the second notch are opposite to and connected along the first direction. The second notch is selectively opened or closed to connect or separate the extraction chamber and the transfer chamber. When the second notch is open, the conveying mechanism transports the sample to be tested to the transfer chamber through the first notch and the second notch.

3. The library construction system for gene detection according to claim 2, characterized in that, The first sidewall is movably provided with a first sealing door, which is used to open or close the second notch.

4. The library construction system for gene detection according to claim 3, characterized in that, The first sealing door is slidably disposed on the first sidewall along the second direction to open or close the second notch, wherein the first direction and the second direction are perpendicular.

5. The library construction system for gene detection according to claim 3, characterized in that, The first extraction chamber sidewall also has a first through hole, the first through hole and the first notch are adjacent to and connected along the second direction, the first sidewall has a second through hole, the second through hole and the second notch are adjacent to and connected along the second direction, the first through hole and the second through hole are opposite to and connected along the first direction, and the conveying mechanism passes through the first through hole and the second through hole, the first direction and the second direction are perpendicular.

6. The library construction system for gene detection according to claim 5, characterized in that, The first sealing door has an abutting end, and a first sealing element is fixedly provided on the abutting end. When the first sealing door closes the second notch, the first sealing element abuts and seals with the conveying mechanism.

7. The library construction system for gene detection according to claim 5, characterized in that, Along the third direction, the two sidewalls of the conveying mechanism are sealed to the transfer chamber and the extraction chamber, and the first direction, the second direction and the third direction are perpendicular to each other.

8. The library construction system for gene detection according to claim 2, characterized in that, A second sealing element is fixed between the first sidewall and the first extraction chamber sidewall. The second sealing element abuts against both the first sidewall and the first extraction chamber sidewall. The second sealing element is arranged around the second notch along the circumference of the second notch, and also around the first notch along the circumference of the first notch.

9. The library construction system for gene detection according to claim 8, characterized in that, A third sealing element is fixed between the first sidewall and the first extraction chamber sidewall. The third sealing element abuts against both the first sidewall and the first extraction chamber sidewall. The third sealing element is located on the side of the second sealing element away from the first notch. The third sealing element is arranged around the second sealing element along its circumference.

10. The library construction system for gene detection according to claim 9, characterized in that, The third sealing element has a first sub-sealing strip, a second sub-sealing strip, and a third sub-sealing strip. The second sub-sealing strip is connected between the first sub-sealing strip and the third sub-sealing strip. The first sub-sealing strip and the third sub-sealing strip both extend along a second direction and are respectively disposed on two opposite side edges of the first sidewall along a third direction. Along the second direction, the second sub-sealing strip is disposed on the upper edge of the first sidewall. The first direction, the second direction, and the third direction are perpendicular to each other.

11. The library construction system for gene detection according to claim 10, characterized in that, The third sealing element also has a fourth sub-sealing strip and a fifth sub-sealing strip. Along the third direction, the fourth sub-sealing strip and the fifth sub-sealing strip are respectively located on both sides of the conveying mechanism. Along the second direction, the lower end of the first sub-sealing strip is connected to the fourth sub-sealing strip, and the lower end of the third sub-sealing strip is connected to the fifth sub-sealing strip. Both the fourth and fifth sub-sealing strips extend along the third direction and are disposed on the lower edge of the first sidewall. The fourth and fifth sub-sealing strips respectively abut and seal against the two sidewalls of the conveying mechanism.

12. The library construction system for gene detection according to any one of claims 2-11, characterized in that, The warehouse construction warehouse has a first warehouse construction warehouse sidewall opposite to the transfer warehouse, and the transfer warehouse has a second sidewall opposite to the first warehouse construction warehouse sidewall. The first warehouse construction warehouse sidewall and the first extraction warehouse sidewall have the same structure, the second sidewall and the first sidewall have the same structure, and the assembly method of the first warehouse construction warehouse sidewall and the second sidewall is the same as the assembly method of the first extraction warehouse sidewall and the first sidewall.

13. The library construction system for gene detection according to claim 11, characterized in that, At least one side wall of the extraction chamber is provided with a first door, the first door being used to open or close the extraction chamber, and / or At least one side wall of the warehouse is provided with a second door, which is used to open or close the warehouse.

14. The library construction system for gene detection according to claim 13, characterized in that, The first and second compartment doors are identical.

15. The library construction system for gene detection according to claim 14, characterized in that, Both the first and second compartment doors include a door frame and a movable door. The door frame has a pick-up and put-out hole, and the movable door is movably disposed on the door frame to open or close the pick-up and put-out hole.

16. The library construction system for gene detection according to claim 15, characterized in that, The movable door and the door frame are disposed opposite to each other and are slidably disposed on the door frame along a second direction, which is perpendicular to the first direction. A fourth sealing member is fixedly provided on the surface of the door frame facing the movable door, and the fourth sealing member abuts against the movable door. A fifth sealing member is fixedly provided on the surface of the movable door facing the door frame, and the fifth sealing member abuts against the door frame. When the movable door closes the pick-up and put-out hole, the fourth sealing member and the fifth sealing member are arranged around the pick-up and put-out hole.

17. The library construction system for gene detection according to claim 16, characterized in that, The fourth sealing element includes: a sixth sub-sealing strip and two seventh sub-sealing strips. Along the second direction, the sixth sub-sealing strip is located at the upper edge of the door frame, and the two seventh sub-sealing strips extend along the second direction and are respectively located at the two side edges of the door frame. The sixth sub-sealing strip is connected between the two fifth sub-sealing strips. The fifth sealing element includes an eighth sub-sealing strip and two ninth sub-sealing strips. Along the second direction, the eighth sub-sealing strip is located at the lower edge of the movable door, and the two ninth sub-sealing strips extend along the second direction and are respectively located at the two side edges of the movable door. The eighth sub-sealing strip is connected between the two ninth sub-sealing strips, and the seventh sub-sealing strip and the corresponding ninth sub-sealing strip are arranged along the width direction of the pick-up and drop-out hole.

18. The library construction system for gene detection according to any one of claims 1-11, characterized in that, The air pressure inside the extraction chamber is greater than the air pressure inside the warehouse construction chamber.

19. The library construction system for gene detection according to any one of claims 1-11, characterized in that, The air pressure inside the transfer chamber is lower than the air pressure inside the extraction chamber but higher than the air pressure inside the warehouse construction chamber.

20. The library construction system for gene detection according to any one of claims 1-11, characterized in that, The extraction chamber has a first gas driving component and a first filter element. The first gas driving component is used to drive gas into and out of the extraction chamber to regulate the gas pressure inside the extraction chamber. The first filter element is used to filter the gas flowing into the extraction chamber.

21. The library construction system for gene detection according to any one of claims 1-11, characterized in that, The gas-building chamber has a second gas-driven component and a second filter. The second gas-driven component is used to drive gas into and out of the gas-building chamber to regulate the gas pressure inside the gas-building chamber. The second filter is used to filter the gas flowing into the gas-building chamber.

22. The library construction system for gene detection according to any one of claims 1-11, characterized in that, The conveying mechanism includes a drive mechanism, a guide rail, and a placement platform. The guide rail passes through the transfer chamber along the first direction, and both ends of the guide rail extend into the extraction chamber and the silo building chamber, respectively. The placement platform is located outside the guide rail and is used to place the sample to be tested. At least a portion of the drive mechanism is disposed inside the guide rail and is kinetically connected to the placement platform. The drive mechanism is used to drive the placement platform to reciprocate relative to the guide rail along the first direction, so that the placement platform moves into one of the extraction chamber, the silo building chamber, and the transfer chamber.

23. The library construction system for gene detection according to claim 22, characterized in that, The guide rail has a guide rail wall facing the placement platform, and the guide rail wall forms a clearance hole extending along the first direction. The driving mechanism includes a driving rod that passes through the clearance hole. The clearance hole is provided with a sixth sealing element for sealing the clearance hole. The sixth sealing element includes a first sealing strip and a second sealing strip. The first sealing strip and the second sealing strip both extend along the first direction and are located on both sides of the driving rod. The first sealing strip and the second sealing strip both abut against the driving rod for sealing.

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