A nucleic acid purification column adapter

Abstract

This utility model discloses a nucleic acid purification column adapter, including an adapter body that attaches to the bottom of a flat-bottomed purification column. The adapter body is divided into four parts from bottom to top: an interface section, a transition section, a main body section, and an outer edge section. The interface section, transition section, main body section, and outer edge section are all made of medical-grade polypropylene and are integrally injection molded. The interface section has an inner diameter of 2.0 mm, the transition section is a conical structure with an inclination angle of 45°, the main body section has an inner diameter of 24.2 mm, and its height is 12 mm. The outer edge section above the main body section has an outward-curving arc-shaped cross-section. This nucleic acid purification column adapter, when assembled to the bottom of the flat-bottomed purification column, transforms the column into a pointed-bottomed purification column, allowing it to be placed in a negative pressure device for separation. After removing the adapter, the flat-bottomed purification column can be placed in a centrifuge for separation, achieving optimal purification results through dual separation.

Description

Technical Field

[0001] This utility model relates to the field of medical testing technology, specifically a nucleic acid purification column adapter. Background Technology

[0002] Nucleic acid purification columns are core consumables used in molecular biology experiments for the rapid extraction and purification of DNA or RNA. They are usually in the form of small plastic columns and are used in conjunction with centrifuges or vacuum pumps.

[0003] In the prior art, Chinese patent application number CN202010580207.6 discloses a nucleic acid purification column, including a purification column body, column reinforcing ribs, and a purification filter membrane. The purification column body has a hollow structure with openings at both ends. One end of the purification column body forms a sample inlet, and the opposite end forms a sample outlet. The column reinforcing ribs have through holes and are connected to the inner wall of the purification column body and close to the sample outlet. The surface of the column reinforcing ribs facing the sample inlet has anti-detachment protrusions. The purification filter membrane is disposed in the purification column body, contacts the column reinforcing ribs, and is engaged with the anti-detachment protrusions.

[0004] In the prior art, Chinese Patent Application No. CN201720932331.8 discloses a nucleic acid purification column, including a purification column body, a sample inlet at the top and a sample outlet at the bottom, a detachable annular fixing frame at the sample inlet, a pipette front portion passing through the central hole of the annular fixing frame, and a pipette rear portion being secured at the central hole of the annular fixing frame.

[0005] The existing purification columns are generally of two types: flat bottom (CN202010580207.6) and pointed bottom (CN201720932331.8). The flat bottom is suitable for centrifugal separation (but it cannot be matched with negative pressure separation equipment), while the pointed bottom is suitable for negative pressure separation (but a large amount of residue will appear during centrifugation). However, in actual use, a single separation method often cannot achieve the best separation effect. Therefore, it is urgent to design an adapter so that the flat bottom purification column can be used for both centrifugal separation and negative pressure separation. Utility Model Content

[0006] The purpose of this invention is to provide a nucleic acid purification column adapter to solve the problem mentioned in the background art that it cannot simultaneously adapt to centrifugal separation and negative pressure separation.

[0007] To achieve the above objectives, this utility model provides the following technical solution: a nucleic acid purification column adapter, comprising an adapter body that is attached to the bottom of a flat-bottomed purification column. The adapter body is divided into four parts from bottom to top: an interface section, a transition section, a main body section, and an outer edge section. The interface section, transition section, main body section, and outer edge section are made of medical-grade polypropylene and are integrally injection molded.

[0008] Preferably, the inner diameter of the interface section is 2.0 mm, and the transition section is a tapered structure with an inclination angle of 45°.

[0009] Preferably, the inner diameter of the main body segment is 24.2 mm, the height of the main body segment is 12 mm, and the cross-section of the outer edge segment above the main body segment is an outward-curved arc structure.

[0010] Preferably, anti-slip pads with equal spacing are fixedly installed on the outer surface of the adapter body, and the anti-slip pads are made of silicone material with a rough surface.

[0011] Preferably, an inwardly protruding limiting boss is fixedly installed on the inner side of the bottom of the main body segment.

[0012] Preferably, a first sealing ring and a second sealing ring are provided on the inner wall of the main body section from top to bottom, and both the first sealing ring and the second sealing ring are fixedly installed in the receiving groove opened on the inner wall of the main body section.

[0013] Preferably, the interface segment has uniformly arranged spiral grooves inside.

[0014] Compared with the prior art, the beneficial effects of this utility model are: the nucleic acid purification column adapter adopts a novel structural design, the specific details of which are as follows:

[0015] 1. After the adapter body is assembled at the bottom of the flat-bottomed purification column, the flat-bottomed purification column is transformed into a pointed-bottomed purification column, which can then be placed in a negative pressure device for separation. After removing the adapter, the flat-bottomed purification column can be placed in a centrifuge for separation, thus achieving the best purification effect through double separation.

[0016] 2. The inner wall of the main body section is equipped with the first sealing ring and the second sealing ring through the receiving groove. When docking with the flat-bottomed purification column, the first sealing ring and the second sealing ring are squeezed to achieve an effective sealing effect. In addition, the limiting protrusion on the inner side of the bottom of the main body section is used to restrict the downward movement of the flat-bottomed purification column, so as to prevent the flat-bottomed purification column from being too tightly adsorbed with the adapter during negative pressure separation.

[0017] Furthermore, the outer edge of the main body section is an outward-curving arc structure to increase the opening area and facilitate the docking between the flat-bottomed purification column and the adapter body.

[0018] Furthermore, a spiral groove structure is provided on the inner wall of the interface end, which can accelerate the flow of internal liquid. Attached Figure Description

[0019] Figure 1 This is a schematic diagram of the overall assembled structure of this utility model;

[0020] Figure 2 This is a schematic diagram of the main structure of the adapter of this utility model;

[0021] Figure 3 This is a schematic cross-sectional view of the adapter body of this utility model;

[0022] Figure 4 This is a schematic diagram of the limiting boss structure of this utility model;

[0023] Figure 5 This utility model Figure 4 Enlarged structural diagram at point A in the middle.

[0024] In the diagram: 1. Adapter body; 101. Interface section; 102. Transition section; 103. Main body section; 104. Outer edge section; 2. Flat-bottomed purification column; 3. Anti-slip pad; 4. Limiting boss; 5. First sealing ring; 6. Second sealing ring; 7. Receiving groove; 8. Spiral groove. Detailed Implementation

[0025] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the protection scope of the present utility model.

[0026] Example 1: To achieve the purpose of applying negative pressure separation (negative pressure filtration mode), this example provides the following technical solution, specifically disclosing: an adapter body 1 that is connected to the bottom of the flat-bottomed purification column 2, such as... Figures 1-3 As shown: The adapter body 1 is divided into four parts from bottom to top: interface section 101, transition section 102, main body section 103, and outer edge section 104. Interface section 101, transition section 102, main body section 103, and outer edge section 104 are all made of medical-grade polypropylene and are integrally injection molded. The inner diameter of interface section 101 is 2.0mm, and transition section 102 is a tapered structure with an inclination angle of 45°. The inner diameter of main body section 103 is 24.2mm, and the height of main body section 103 is 12mm. The outer edge section 104 above main body section 103 has an outwardly flared arc-shaped cross-section. An inwardly protruding limiting boss 4 is fixedly installed on the inner side of the bottom of main body section 103. Figures 4-5As shown: The inner wall of the main body section 103 is provided with a first sealing ring 5 and a second sealing ring 6 from top to bottom, and the first sealing ring 5 and the second sealing ring 6 are both fixedly installed in the receiving groove 7 opened in the inner wall of the main body section 103. The interface section 101 has evenly arranged spiral grooves 8 inside.

[0027] In use, first take out the adapter body 1, then align the adapter body 1 with the bottom of the flat-bottomed purification column 2, and then insert the flat-bottomed purification column 2 into the adapter body 1 (during this process, the outer edge section 104 above the main body section 103 is used to expand the area for easy insertion). Finally, the bottom of the flat-bottomed purification column 2 abuts against the limiting boss 4 on the inner side of the bottom of the main body section 103. The limiting boss 4 restricts the flat-bottomed purification column 2 from moving downward. At the same time, the outer wall of the flat-bottomed purification column 2 squeezes the first sealing ring 5 and the second sealing ring 6 (the first sealing ring 5 and the second sealing ring 6 are installed inside the receiving groove 7. When the first sealing ring 5 and the second sealing ring 6 are squeezed, the receiving groove 7 provides a certain protection to them, preventing them from being squeezed excessively for a long time and affecting the sealing effect). Thus, the first sealing ring 5 and the second sealing ring 6 achieve the purpose of sealing the device. Then, insert the interface section 101 into the negative pressure separation device, and use the negative pressure adsorption to separate the substances in the flat-bottomed purification column 2. During this process, the spiral groove 8 on the inner wall of the interface section 101 can increase the liquid flow rate.

[0028] Example 2: To achieve the purpose of applicable centrifugal separation (high-speed centrifugation mode), this example provides the following technical solution, which is specifically disclosed, such as... Figure 2 As shown: Anti-slip pads 3 are fixedly installed on the outer surface of the adapter body 1 at equal intervals, and the anti-slip pads 3 are made of silicone material with a rough surface.

[0029] After the negative pressure separation is completed, remove the device from the negative pressure equipment, and then remove the adapter body 1 from the bottom of the flat-bottomed purification column 2 (when pulling out the adapter body 1, the operator pinches the anti-slip pad 3 to increase friction by using the rough surface of the anti-slip pad 3 to avoid slipping). Then, put the flat-bottomed purification column 2 into the centrifuge and separate it by centrifugation.

[0030] In the description of this utility model, unless otherwise stated, "a plurality of" means two or more; the terms "upper," "lower," "left," "right," "inner," "outer," "front end," "rear end," "head," "tail," etc., indicate the orientation or positional relationship based on the orientation or positional relationship shown in the accompanying drawings, and are only for the convenience of describing this utility model and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation, and therefore should not be construed as a limitation of this utility model. In addition, the terms "first," "second," "third," etc., are used for descriptive purposes only and should not be construed as indicating or implying relative importance.

[0031] Although embodiments of the present invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made to these embodiments without departing from the principles and spirit of the present invention, the scope of which is defined by the appended claims and their equivalents.

Claims

1. A nucleic acid purification column adapter, comprising an adapter body (1) docked to the bottom of a flat-bottomed purification column (2), characterized in that, Also includes: The adapter body (1) is divided into four parts from bottom to top: interface section (101), transition section (102), main body section (103) and outer edge section (104). The interface section (101), transition section (102), main body section (103) and outer edge section (104) are made of medical grade polypropylene and are integrally injection molded.

2. The nucleic acid purification column adapter according to claim 1, characterized in that: The inner diameter of the interface section (101) is 2.0 mm, and the transition section (102) is a tapered structure with an inclination angle of 45°.

3. The nucleic acid purification column adapter according to claim 1, characterized in that: The inner diameter of the main body segment (103) is 24.2 mm, and the height of the main body segment (103) is 12 mm. The cross-section of the outer edge segment (104) above the main body segment (103) is an outward-curved arc structure.

4. A nucleic acid purification column adapter according to claim 1, characterized in that: The adapter body (1) has anti-slip pads (3) that are evenly spaced and fixedly installed on its outer surface, and the anti-slip pads (3) are made of silicone material with a rough surface.

5. A nucleic acid purification column adapter according to claim 3, characterized in that: The bottom inner side of the main body section (103) is fixedly installed with an inwardly protruding limiting boss (4).

6. A nucleic acid purification column adapter according to claim 5, characterized in that: The inner wall of the main body section (103) is provided with a first sealing ring (5) and a second sealing ring (6) from top to bottom, and the first sealing ring (5) and the second sealing ring (6) are both fixedly installed in the receiving groove (7) opened on the inner wall of the main body section (103).

7. A nucleic acid purification column adapter according to claim 1, characterized in that: The interface segment (101) has evenly arranged spiral grooves (8) inside.

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