A rust fungus preservation processing assembly
By using vacuuming and flame sealing technology with negative pressure equipment and sealing tube components, the problems of complex and costly rust fungus preservation operations have been solved, enabling long-term preservation and activity maintenance, and significantly reducing preservation costs.
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- INST OF PLANT PROTECTION GANSU ACAD OF AGRI SCI
- Filing Date
- 2025-08-15
- Publication Date
- 2026-07-14
AI Technical Summary
Existing methods for preserving rust fungi are complex to operate, costly, and unsuitable for long-term preservation. Suspension preservation is suitable for short-term but not for long-term research, while liquid nitrogen preservation is expensive, although it can preserve rust fungi for a long time.
Using negative pressure equipment and a specially structured sealing tube assembly, rust fungi are preserved by vacuuming and flame sealing. The sealing tube assembly includes a vertical tube, a protruding nozzle, a connecting sleeve, and a sealing tube. The sealing tube is designed as a first tube section, a reduced-diameter tube section, and a second tube section. The negative pressure equipment and flame sealing are used to achieve long-term preservation of rust fungi.
It enables long-term preservation and activity maintenance of rust fungi, with a preservation time of up to five years or more. The operation is simple and inexpensive, reducing preservation costs.
Smart Images
Figure CN224494185U_ABST
Abstract
Description
Technical Field
[0001] This utility model relates to the field of microbial preservation technology, and in particular to a preservation and processing component for rust fungi. Background Technology
[0002] Rust fungi are an important class of plant pathogenic microorganisms, and their preservation is of great significance for plant pathology research, disease control, and biodiversity conservation. Currently, the main methods for preserving rust fungi include suspension preservation and liquid nitrogen preservation. However, existing technologies have some limitations. For example, while suspension preservation reduces the difficulty and cost of urediniospore preservation and achieves short-term "immediate" preservation, it is only suitable for short-term storage (1-12 months) and not for long-term preservation and research. Liquid nitrogen preservation allows for long-term preservation of rust fungi, but it is costly, complex, and requires regular replenishment of liquid nitrogen. Therefore, there is an urgent need for a simple, low-cost rust fungi preservation and processing component that can maintain the activity of rust fungi for extended periods. Utility Model Content
[0003] To solve one of the above-mentioned technical problems, this utility model provides a rust fungus preservation and processing component.
[0004] The technical solution adopted in this application is as follows:
[0005] This application provides a rust fungus preservation and processing component, comprising:
[0006] Negative pressure equipment;
[0007] The bracket has a vertical tube and multiple protrusions. The vertical tube is connected to the negative pressure device and has a cavity communicating with the negative pressure device. Each of the protrusions is disposed on the vertical tube and is arranged sequentially along the length of the vertical tube. Each protrusion is connected to a connecting sleeve.
[0008] Multiple sealing pipes, each sealing pipe having a first pipe section, a second pipe section, and a reduced-diameter pipe section, the reduced-diameter pipe section being located between the first pipe section and the second pipe section, the outer diameter of the reduced-diameter pipe section being smaller than that of the first pipe section and the second pipe section, the reduced-diameter pipe section being connected to the first pipe section and the second pipe section respectively, the second pipe section having an opening at one end opposite to the first pipe section, the length of the second pipe section being shorter than that of the first pipe section, the second pipe section of each sealing pipe being detachably connected to a corresponding connecting sleeve to communicate with the cavity of the vertical pipe.
[0009] Optionally, the length ratio of the first pipe section, the reduced-diameter pipe section, and the second pipe section is (4.5-6):1:(2-3.5).
[0010] Optionally, the ratio of the outer diameter of the reduced-diameter pipe section to that of the first or second pipe section is 1:(1.5 to 3).
[0011] Optionally, the bracket includes at least two rows of protrusions, with each row of protrusions arranged sequentially at intervals around the circumference of the vertical tube, and each protrusion in each row arranged sequentially at intervals along the length of the vertical tube.
[0012] Optionally, the rust preservation and processing assembly includes a pressure sensor connected to the vertical pipe and communicating with the cavity, the pressure sensor being used to monitor the pressure in the cavity.
[0013] Optionally, the vertical pipe includes a first vertical pipe, a second vertical pipe, and a tee pipe;
[0014] The first vertical pipe is connected to the negative pressure device, and the second vertical pipe is provided with the protruding nozzle;
[0015] The tee pipe has a first pipe body, a second pipe body, and a third pipe body;
[0016] The tee pipe is located between the first vertical pipe and the second vertical pipe, with the first pipe body connected to the first vertical pipe and the second pipe body connected to the second vertical pipe;
[0017] The pressure sensor is connected to the third tube.
[0018] Optionally, the bracket includes a clamp;
[0019] The pressure sensor has an interface tube, and a first flange ring is provided at the end of the interface tube;
[0020] A second flange ring is provided on the third pipe body;
[0021] The clamp is fitted onto the first flange ring and the second flange ring to connect and fix the interface pipe and the third pipe body.
[0022] Optionally, the rust preservation processing components include sealing rings;
[0023] A plug is provided on the third pipe body, and the plug protrudes from the second flange ring;
[0024] The sealing ring is fitted onto the insert;
[0025] With the third tube and the interface tube connected, the insert is inserted into the interface tube, and the sealing ring is compressed and positioned between the first flange ring and the second flange ring.
[0026] Optionally, the second flange ring has a tapered surface on the side opposite to the first flange ring;
[0027] The clamp has a first baffle and a second baffle. The first baffle is attached to the side of the first flange ring away from the second flange ring, and the second baffle is sleeved on the conical surface.
[0028] Optionally, the clamp includes two fasteners;
[0029] The two fasteners are hinged at one end and detachably connected at the other end.
[0030] Each of the aforementioned card holders is provided with the first baffle and the second baffle.
[0031] By adopting the above technical solution, this application has the following beneficial effects:
[0032] Rust fungi can be placed within the first section of the sealing tube in the preservation and processing assembly of this application. During negative pressure operation, the sealing tube can be vacuum-sealed. The sealing tube has a reduced-diameter section, allowing for easy and rapid heat fusion sealing of the first section under flame conditions. The second section of the sealing tube is shorter than the first section, without affecting the fit between the second section and the connecting sleeve. The preservation and processing assembly of this application achieves long-term preservation and activity maintenance of rust fungi. The sealed fungi are easy to transport, handle, and store. Experiments have shown that it can extend the preservation time of rust fungi by more than five years.
[0033] The specific embodiments of this utility model will be described in further detail below with reference to the accompanying drawings. Attached Figure Description
[0034] The accompanying drawings, which form part of this application, are used to provide a further understanding of the present invention. The illustrative embodiments and descriptions of the present invention are used to explain the present invention, but do not constitute an undue limitation of the present invention. Obviously, the drawings described below are merely some embodiments; those skilled in the art can obtain other drawings based on these drawings without creative effort. In the drawings:
[0035] Figure 1 This diagram illustrates the structure of the rust fungus preservation and processing assembly provided in an embodiment of the present disclosure.
[0036] Figure 2 Show Figure 1 Enlarged view of section A in the middle;
[0037] Figure 3 This diagram illustrates the combined structure of the tee tube and the pressure sensor in the rust preservation and processing assembly provided in this embodiment of the present disclosure.
[0038] Figure 4 An exploded view shows the combined structure of the tee and the pressure sensor in the rust preservation and processing assembly provided in this embodiment of the present disclosure;
[0039] Figure 5 This diagram illustrates a three-dimensional structure of the sealing tube in the rust preservation and processing assembly provided in an embodiment of the present disclosure.
[0040] In the diagram: 1. Negative pressure equipment; 2. Support; 211. First vertical pipe; 212. Second vertical pipe; 213. T-pipe; 2131. Third pipe body; 21311. Second flange ring; 21311a. Conical surface; 21312. Insert; 22. Protruding nozzle; 23. Connecting sleeve; 24. Clamp; 241. Fastener; 2411. First baffle; 2412. Second baffle; 242. Bolt; 3. Sealing pipe; 31. First pipe section; 32. Second pipe section; 33. Reduced diameter pipe section; 4. Pressure sensor; 41. Interface pipe; 411. First flange ring; 5. Sealing ring.
[0041] It should be noted that these accompanying drawings and textual descriptions are not intended to limit the scope of the present invention in any way, but rather to illustrate the concept of the present invention to those skilled in the art by referring to specific embodiments. Detailed Implementation
[0042] To make the objectives, technical solutions, and advantages of the embodiments of this utility model clearer, the technical solutions in the embodiments will be clearly and completely described below with reference to the accompanying drawings. The following embodiments are used to illustrate this utility model, but are not intended to limit the scope of this utility model.
[0043] In the description of this utility model, it should be noted that the terms "upper", "lower", "inner", "outer", etc., indicate the orientation or positional relationship based on the orientation or positional relationship shown in the accompanying drawings. They are only for the convenience of describing this utility model and simplifying the description, and do not indicate or imply that the device or component referred to must have a specific orientation, or be constructed and operated in a specific orientation. Therefore, they should not be construed as limitations on this utility model.
[0044] In the description of this utility model, it should be noted that, unless otherwise explicitly specified and limited, the terms "installation" and "connection" should be interpreted broadly. For example, they can refer to a fixed connection, a detachable connection, or an integral connection; they can refer to a mechanical connection or an electrical connection; they can refer to a direct connection or an indirect connection through an intermediate medium. Those skilled in the art can understand the specific meaning of the above terms in this utility model based on the specific circumstances.
[0045] like Figures 1 to 5As shown in the figure, this application provides a rust fungus preservation and processing assembly, including: a negative pressure device 1, a support 2, and multiple sealing tubes 3. The support 2 has a vertical tube and multiple protrusions 22. The vertical tube is connected to the negative pressure device 1 and has a cavity communicating with the negative pressure device 1. Each of the protrusions 22 is disposed on the vertical tube and is arranged sequentially along the length direction of the vertical tube. Each protrusion 22 is connected to a connecting sleeve 23. The sealing pipe 3 has a first pipe section 31, a second pipe section 32, and a reduced-diameter pipe section 33. The reduced-diameter pipe section 33 is located between the first pipe section 31 and the second pipe section 32. The outer diameter of the reduced-diameter pipe section is smaller than that of the first pipe section 31 and the second pipe section 32. The reduced-diameter pipe section 33 is connected to the first pipe section 31 and the second pipe section 32 respectively. The end of the second pipe section 32 facing away from the first pipe section 31 is provided with a pipe opening. The length of the second pipe section 32 is less than that of the first pipe section 31. The second pipe section 32 of each sealing pipe 3 can be detachably connected to the corresponding connecting sleeve 23 to communicate with the cavity of the vertical pipe.
[0046] The support 2 of this application has a vertical tube that extends along the height direction, occupying little indoor floor space. Multiple protrusions 22 can be installed on the vertical tube, which can facilitate the arrangement of a large number of connecting sleeves 23 to simultaneously perform vacuum treatment on multiple sealing tubes 3. The connecting sleeve 23 can be a rubber tube, which is sleeved on the outside of the protrusions 22. When the sealing tube 3 and the connecting sleeve 23 are connected, the connecting sleeve 23 is sleeved on the second section 32 of the sealing tube 3. The opening of the second section 32 is located inside the connecting sleeve 23, so that the cavity of the sealing tube 3 and the vertical tube is sealed and connected.
[0047] Rust fungi can be placed inside the first pipe section 31. During the operation of the negative pressure equipment 1, a vacuum treatment can be performed on the sealing pipe 3. The sealing pipe 3 has a reduced diameter section 33, which can easily and quickly heat-seal the first pipe section 31 under flame action. Figure 1 As shown. The length of the second pipe segment 32 of the sealing tube 3 is shorter than that of the first pipe segment 31, which does not affect the insertion and mating of the second pipe segment 32 and the connecting sleeve 23, and also shortens the length of the sealing tube 3, saving costs. The preservation and processing component of this application realizes the long-term preservation and activity maintenance of rust fungi. The fungi after sealing tube 3 are easy to transport, handle and preserve. Experiments have shown that the preservation time of rust fungi can be extended by more than five years.
[0048] In some possible implementations, the length ratio of the first pipe segment 31, the reduced-diameter pipe segment 33, and the second pipe segment 32 is (4.5–6):1:(2–3.5). The ratio of the outer diameter of the reduced-diameter pipe segment 33 to the outer diameter of the first pipe segment 31 or the second pipe segment 32 is 1:(1.5–3).
[0049] The sealing tube 3 can be a glass tube. Compared to a regular ampere tube, the sealing tube 3 in this application has been optimized in design. Through the aforementioned dimensional ratio constraints, the inner diameter of the reduced-diameter section 33 of the sealing tube 3 is smaller, and the extension length of the reduced-diameter section 33 is longer, allowing for easy and rapid heat fusion sealing of the first section 31 under flame conditions. Figure 1 As shown, this reduces the treatment time of the sealing tube 3 and minimizes damage to the bacteria, enabling long-term preservation and high activity maintenance of rust bacteria.
[0050] In some possible implementations, such as Figure 1 As shown, the support 2 includes at least two rows of protruding nozzles 22, with each row of nozzles 22 arranged sequentially at intervals around the circumference of the vertical tube, and each nozzle 22 in each row arranged sequentially at intervals along the length of the vertical tube. The structural design of the vertical tube in this application allows a large number of nozzles 22 to be arranged on a single vertical tube, enabling simultaneous vacuuming of multiple sealing tubes 3.
[0051] In some possible implementations, such as Figure 1 As shown, the rust preservation and processing assembly includes a pressure sensor 4, which is connected to the vertical pipe and communicates with the cavity. The pressure sensor 4 is used to monitor the air pressure in the cavity. By setting the pressure sensor 4, the air pressure in the cavity inside the vertical pipe can be monitored in real time to ensure that the air pressure inside the vertical pipe reaches the set value. The negative pressure device, also known as a vacuum device, is used to evacuate air, so that the air pressure inside the vertical pipe is maintained at a low value. Usually, when the air pressure monitored by the pressure sensor 4 reaches below 5 Pa, the experimenter can perform flame sealing treatment on the sealing pipe 3. By burning the reduced diameter section 33 of the sealing pipe 3 with a high-temperature flame, the locking section is heat-melted to seal the first section 31, such as... Figure 1 As shown, the first pipe section 31 and the second pipe section 32 eventually separate, and the second pipe section 32 will be connected to the connecting sleeve 23.
[0052] In some possible implementations, such as Figure 1 and Figure 2 As shown, the vertical pipe includes a first vertical pipe 211, a second vertical pipe 212, and a three-way pipe 213. The first vertical pipe 211 is connected to the negative pressure device 1. The second vertical pipe 212 is provided with the protruding nozzle 22. The three-way pipe 213 has a first pipe body, a second pipe body, and a third pipe body 2131. The three-way pipe 213 is located between the first vertical pipe 211 and the second vertical pipe 212. The first pipe body is connected to the first vertical pipe 211, the second pipe body is connected to the second vertical pipe 212, and the air pressure sensor 4 is connected to the third pipe body 2131. In order to achieve accurate and effective monitoring of the air pressure in the cavity of the vertical pipe, this embodiment of the application provides a three-way pipe 213 to connect the air pressure sensor 4 to the vertical pipe.
[0053] In some possible implementation schemes, combined Figures 1 to 4As shown, the bracket 2 includes a clamp 24, the air pressure sensor 4 has an interface tube 41, the end of the interface tube 41 is provided with a first flange ring 411, the third tube body 2131 is provided with a second flange ring 21311, and the clamp 24 is sleeved on the first flange ring 411 and the second flange ring 21311 to connect and fix the interface tube 41 and the third tube body 2131.
[0054] Specifically, the rust preservation and processing component includes a sealing ring 5. A sleeve 21312 is provided on the third tube 2131, protruding from the second flange ring 21311. The sealing ring 5 is fitted onto the sleeve 21312. When the third tube 2131 and the interface tube 41 are connected, the sleeve 21312 is inserted into the interface tube 41, and the sealing ring 5 is compressed and positioned between the first flange ring 411 and the second flange ring 21311. The sealing ring 5 serves to seal the gap between the third tube 2131 and the interface tube 41.
[0055] In some possible implementations, such as Figure 4 As shown, the second flange ring 21311 has a tapered surface 21311a on the side opposite to the first flange ring 411. The clamp 24 has a first baffle 2411 and a second baffle 2412. The first baffle 2411 fits against the side of the first flange ring 411 opposite to the second flange ring 21311, and the second baffle 2412 is fitted onto the tapered surface 21311a. By providing the tapered surface 21311a on the second flange ring 21311, when the second baffle 2412 is fitted onto the tapered surface 21311a, a compressive force can be effectively applied to the tapered surface 21311a, which is beneficial for the first flange ring 411 and the second flange ring 21311 to approach and clamp together to form the sealing ring 5.
[0056] The clamp 24 includes two fasteners 241, one end of which is hinged together, and the other ends of which are detachably connected. Each fastener 241 is provided with a first baffle 2411 and a second baffle 2412. When the two fasteners 241 of the clamp 24 are connected, the first baffles 2411 on the two fasteners 241 form an annulus and are located on the same plane, and the second baffles 2412 on the two fasteners 241 form an annulus and are located on the same plane. The free ends of the two fasteners 241 can be connected and fixed by bolts 242.
[0057] It should be noted that the clamp connection between the interface pipe and the third pipe can be applied to the connection between the first pipe and the first vertical pipe, as well as the connection between the second pipe and the second vertical pipe.
[0058] The following is a brief explanation of the sealed tube preservation method for rust fungi (such as stripe rust fungi):
[0059] Sample preparation: Collect the stripe rust fungus strain and place it in a glass test tube.
[0060] In this step, rust fungi can be inoculated onto wheat seedlings, cultured until the sporulation stage, and then the rust fungi spores can be collected into test tubes.
[0061] Drying treatment: The collected stripe rust spores were placed in a desiccator containing a desiccant and dried at 4°C.
[0062] In this step, the test tube containing the rust fungus is placed in a desiccator and dried at 4°C for 7-10 days until the rust fungus is completely dry.
[0063] Vacuum sealing: After drying, the spore sample is placed in the sealing tube 3 of this application, and the air inside the tube is removed by the negative pressure device 1. After the predetermined vacuum degree is reached, the tube opening is sealed.
[0064] In this step, the dried rust fungus is transferred to the sealing tube 3, and the sealing tube 3 is vacuumed using the negative pressure device 1 to reduce the pressure inside the tube to 5Pa. Then, the sealing tube 3 is sealed with a flame.
[0065] Vacuum sealing and preservation: After completing vacuum sealing step 3, the sample can be stored for a long time under refrigeration, and the activity of the sporophytes can be restored by reculturing.
[0066] In this step, the rust fungus after sealing is stored at 4℃ or -20℃ for a long period of time.
[0067] The rust preservation and processing components of this application have many beneficial effects:
[0068] The rust fungus was dried before being sealed into the first section 31 of the sealing tube 3. During the sealing process, vacuum treatment was performed, significantly extending the preservation time of the rust fungus; experiments showed that the preservation time could reach more than 5 years. The activity loss rate of the sealed rust fungus was better than that of existing technologies. The preservation and processing component of this application is simple to operate in sealing tests, and the fungus after sealing in tube 3 is easy to transport, handle, and preserve. The preservation and processing component of this application has a simple structure, low cost, and significantly reduced cost compared to liquid nitrogen preservation.
[0069] The above description is merely a preferred embodiment of the present utility model and is not intended to limit the present utility model in any way. Although the present utility model has been disclosed above with reference to a preferred embodiment, it is not intended to limit the present utility model. Any person skilled in the art can make some modifications or alterations to the above-described technical content to create equivalent embodiments without departing from the scope of the present utility model. Any simple modifications, equivalent changes, and alterations made to the above embodiments based on the technical essence of the present utility model without departing from the scope of the present utility model shall still fall within the scope of the present utility model.
Claims
1. A component for preserving and processing rust fungi, characterized in that, include: Negative pressure equipment; The bracket has a vertical tube and multiple protrusions. The vertical tube is connected to the negative pressure device and has a cavity communicating with the negative pressure device. Each of the protrusions is disposed on the vertical tube and is arranged sequentially along the length of the vertical tube. Each protrusion is connected to a connecting sleeve. Multiple sealing pipes, each sealing pipe having a first pipe section, a second pipe section, and a reduced-diameter pipe section, the reduced-diameter pipe section being located between the first pipe section and the second pipe section, the outer diameter of the reduced-diameter pipe section being smaller than that of the first pipe section and the second pipe section, the reduced-diameter pipe section being connected to the first pipe section and the second pipe section respectively, the second pipe section having an opening at one end opposite to the first pipe section, the length of the second pipe section being shorter than that of the first pipe section, the second pipe section of each sealing pipe being detachably connected to a corresponding connecting sleeve to communicate with the cavity of the vertical pipe.
2. The rust fungus preservation and processing component according to claim 1, characterized in that, The length ratio of the first pipe section, the reduced-diameter pipe section, and the second pipe section is (4.5-6):1:(2-3.5).
3. The rust fungus preservation and processing component according to claim 1, characterized in that, The ratio of the outer diameter of the reduced-diameter pipe section to that of the first or second pipe section is 1:(1.5 to 3).
4. The rust fungus preservation and processing component according to claim 1, characterized in that, The bracket includes at least two rows of protruding nozzles, with each row of nozzles arranged sequentially at intervals around the circumference of the vertical tube, and each nozzle in each row arranged sequentially at intervals along the length of the vertical tube.
5. The rust fungus preservation and processing component according to claim 1, characterized in that, It includes a pressure sensor connected to the vertical pipe and communicating with the cavity, and the pressure sensor is used to monitor the pressure of the cavity.
6. The rust fungus preservation and processing assembly according to claim 5, characterized in that, The vertical pipe includes a first vertical pipe, a second vertical pipe, and a tee pipe; The first vertical pipe is connected to the negative pressure device, and the second vertical pipe is provided with the protruding nozzle; The tee pipe has a first pipe body, a second pipe body, and a third pipe body; The tee pipe is located between the first vertical pipe and the second vertical pipe, with the first pipe body connected to the first vertical pipe and the second pipe body connected to the second vertical pipe; The pressure sensor is connected to the third tube.
7. The rust fungus preservation and processing component according to claim 6, characterized in that, The bracket includes clamps; The pressure sensor has an interface tube, and a first flange ring is provided at the end of the interface tube; A second flange ring is provided on the third pipe body; The clamp is fitted onto the first flange ring and the second flange ring to connect and fix the interface pipe and the third pipe body.
8. The rust fungus preservation and processing assembly according to claim 7, characterized in that, Including sealing rings; A plug is provided on the third pipe body, and the plug protrudes from the second flange ring; The sealing ring is fitted onto the insert; With the third tube and the interface tube connected, the insert is inserted into the interface tube, and the sealing ring is compressed and positioned between the first flange ring and the second flange ring.
9. The rust fungus preservation and processing assembly according to claim 7, characterized in that, The second flange ring has a tapered surface on the side opposite to the first flange ring; The clamp has a first baffle and a second baffle. The first baffle is attached to the side of the first flange ring away from the second flange ring, and the second baffle is sleeved on the conical surface.
10. The rust fungus preservation and processing assembly according to claim 9, characterized in that, The clamp includes two clamping parts; The two fasteners are hinged at one end and detachably connected at the other end. Each of the aforementioned card holders is provided with the first baffle and the second baffle.