A dairy cow genome magnetic bead separation device

Abstract

The utility model discloses a kind of dairy cow genome magnetic bead separation devices, including organism, the outside wall of the organism is equipped with PLC controller. The utility model uses, the current intensity of electromagnet inside installation in magnetic force frame is controlled by programmable power supply, and magnetic field intensity will change linearly with current, that is, the greater the current, the stronger the magnetic field, furthermore, utilize Hall effect sensor installed in magnetic field, based on Hall effect, when current passes through semiconductor wafer and is in magnetic field, wafer two sides can generate Hall voltage proportional to magnetic field intensity, by Hall effect sensor measurement this voltage can utilize PLC controller to convert magnetic field intensity, this is convenient for PLC controller intelligent regulation and control magnetic field intensity, optimize the effect of this step of attracting nucleic acid carrying magnetic bead, avoid that magnetic bead cannot be completely adsorbed due to magnetic force is too weak, affect the separation discharge of waste such as cell fragments, and magnetic force is too strong, and it is easy to cause magnetic bead and sample test tube damage.

Description

Technical Field

[0001] This utility model relates to the field of dairy cow genome separation technology, specifically a dairy cow genome magnetic bead separation device. Background Technology

[0002] Bovine genomic DNA separation devices are widely used in molecular biology research such as gene analysis and breeding screening. They are devices specifically designed to separate and purify genomic DNA from biological samples such as bovine blood, tissues, and semen. In operation, magnetic beads with special functional groups on their surface are subjected to specific buffer conditions, and these groups form specific adsorption with DNA molecules, causing the DNA to adhere firmly to the surface of the magnetic beads. For example, silanol groups are bound to DNA phosphate groups through hydrogen bonds, thereby enabling efficient and high-purity extraction of bovine genomic DNA.

[0003] Current bovine genome magnetic bead separation devices often require users to manually remove waste liquid from the sample to obtain individual magnetic beads carrying nucleic acids, facilitating subsequent washing and extraction. This results in the inability of the bovine genome magnetic bead separation device to perform batch and efficient separation processing, and it is also difficult to precisely control the intensity of magnetic bead attraction, affecting the separation effect. Based on this, we propose a novel bovine genome magnetic bead separation device. Utility Model Content

[0004] The purpose of this invention is to provide a bovine genome magnetic bead separation device to solve the problems of low efficiency, inability to process in batches, and difficulty in adjusting magnetic field strength mentioned in the background art.

[0005] To achieve the above objectives, this utility model provides the following technical solution: a bovine genome magnetic bead separation device, comprising a body, a PLC controller mounted on the outer wall of the body, a magnetic frame fixed at the bottom of the body, electromagnets uniformly installed inside the magnetic frame, a shielded housing fixed inside the body on one side of the magnetic frame, a programmable power supply installed inside the shielded housing, a Hall effect sensor installed inside the body above the magnetic frame, positioning slide rails fixed at both ends inside the magnetic frame, an integrated carrier slidably connected between adjacent positioning slide rails, sample tubes uniformly mounted on the integrated carrier, magnetic beads uniformly placed inside the sample tubes, a cap threadedly connected to the top of the sample tubes, a waste liquid extraction seat fixed at the top of the body, a waste liquid collection tank mounted on one side of the body, a drain pipe between the waste liquid collection tank and the waste liquid extraction seat, and a corrosion-resistant pump mounted on one side of the waste liquid collection tank.

[0006] As a further technical solution of this utility model, a connecting pipe fitting that extends vertically to the bottom of the sample tube is fixed on the cover, and both ends of the bottom of the waste liquid extraction seat are provided with a matching interface to the connecting pipe fitting.

[0007] As a further technical solution of this utility model, both the connecting pipe and the connecting interface are provided with a rubber sealing layer.

[0008] As a further technical solution of this utility model, ultraviolet sterilization lamps are installed at both ends of the body.

[0009] As a further technical solution of this utility model, the top of the integrated carrier is uniformly provided with insertion and placement ports that match the sample tubes.

[0010] As a further technical solution of this utility model, telescopic cylinders are installed at both ends of the machine body, and the output end of the telescopic cylinder extends laterally into the interior of the positioning slide rail.

[0011] As a further technical solution of this utility model, the bottom of both ends of the integrated shelf is provided with positioning slide bars that match the positioning slide rail.

[0012] As a further technical solution of this utility model, the inner wall of the body is provided with a nano-copper antibacterial coating, and the inside of the waste liquid collection tank is provided with a filter layer.

[0013] Compared with the prior art, the beneficial effects of this utility model are:

[0014] By incorporating a programmable power supply, the device optimizes its performance. Users operate the PLC controller, controlling the current intensity of the electromagnets installed inside the magnetic frame via the programmable power supply. The magnetic field strength changes linearly with the current; that is, the greater the current, the stronger the magnetic field. Furthermore, a Hall effect sensor installed within the magnetic field is used. Based on the Hall effect, when current passes through a semiconductor wafer in a magnetic field, a Hall voltage proportional to the magnetic field strength is generated on both sides of the wafer. By measuring this voltage with the Hall effect sensor, the PLC controller can calculate the magnetic field strength. This allows the PLC controller to intelligently adjust the magnetic field strength, optimizing the effect of attracting magnetic beads carrying nucleic acids. It avoids situations where the magnetic force is too weak, causing the magnetic beads to not be completely adsorbed, which would affect the separation and discharge of waste such as cell debris. On the other hand, excessive magnetic force can easily damage the magnetic beads and sample tubes.

[0015] By incorporating an integrated carrier rack, the device optimizes its structure. Firstly, users can utilize the sliding connection formed by the positioning slider and rail to slide the integrated carrier rack, containing two rows of sample tubes filled with bovine gene samples and magnetic beads, into the machine. Then, two telescopic cylinders are activated, their output ends extending and pressing against the positioning slider inside the rail to achieve a locking mechanism, improving stability. After the integrated carrier rack is assembled into the machine, the connecting pipe at the top of the sample tube aligns perfectly with the interface on the waste liquid extraction seat, facilitating subsequent waste liquid extraction and separation operations. This enables the device to achieve automated batch separation and processing with high efficiency. Secondly, after the magnetic beads carrying nucleic acids inside the sample tube are attracted and fixed, the corrosion-resistant pump starts, automatically evacuating the waste material inside the sample tube through the drain pipe and waste liquid extraction seat. This allows for automated and efficient separation of bovine genomes. Attached Figure Description

[0016] Figure 1 This is a frontal cross-sectional view of the present invention.

[0017] Figure 2 This is a side view of the structure of this utility model;

[0018] Figure 3 This is a top view of a partial cross-sectional structure of the sample tube of this utility model;

[0019] Figure 4 This is a top view cross-sectional structural diagram of the magnetic frame of this utility model;

[0020] Figure 5 This utility model Figure 1 Enlarged structural diagram at point A in the middle.

[0021] In the diagram: 1. Main body; 2. Ultraviolet sterilization lamp; 3. Connecting interface; 4. Waste liquid extraction seat; 5. Connecting pipe fitting; 6. Cover; 7. Sample tube; 8. Magnetic rack; 9. Telescopic cylinder; 10. PLC controller; 11. Drain pipe; 12. Waste liquid collection tank; 13. Corrosion-resistant pump; 14. Magnetic bead; 15. Shielded housing; 16. Programmable power supply; 17. Electromagnet; 18. Positioning slider; 19. Positioning slide rail; 20. Integrated rack; 21. Hall effect sensor. Detailed Implementation

[0022] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. All other embodiments obtained by those skilled in the art based on the embodiments of the present utility model without creative effort are within the scope of protection of the present utility model.

[0023] Please see Figure 1-5An embodiment of this utility model is provided: a bovine genome magnetic bead separation device, including a body 1, a PLC controller 10 installed on the outer wall of the body 1, a magnetic frame 8 fixed at the bottom inside the body 1, an electromagnet 17 uniformly installed inside the magnetic frame 8, a shielded shell 15 fixed inside the body 1 on one side of the magnetic frame 8, a programmable power supply 16 installed inside the shielded shell 15, and a Hall effect sensor 21 installed inside the body 1 above the magnetic frame 8.

[0024] Both ends of the magnetic rack 8 are fixed with positioning slide rails 19. An integrated carrier rack 20 is slidably connected between adjacent positioning slide rails 19. Sample tubes 7 are evenly mounted on the integrated carrier rack 20. Magnetic beads 14 are evenly placed inside the sample tubes 7.

[0025] Specifically, such as Figure 1 , Figure 2 , Figure 3 and Figure 4 As shown, the user operates the PLC controller 10 and controls the current intensity of the electromagnet 17 installed inside the magnetic rack 8 through the programmable power supply 16. The magnetic field strength changes linearly with the current, that is, the greater the current, the stronger the magnetic field. Furthermore, using the Hall effect sensor 21 installed in the magnetic field, based on the Hall effect, when the current passes through the semiconductor thin film and is in the magnetic field, a Hall voltage proportional to the magnetic field strength is generated on both sides of the thin film. By measuring this voltage through the Hall effect sensor 21, the PLC controller 10 can calculate the magnetic field strength. This facilitates the PLC controller 10 to intelligently control the magnetic field strength, optimize the effect of attracting the magnetic beads 14 carrying nucleic acid, and avoid the magnetic force being too weak, which would cause the magnetic beads 14 to not be completely attracted, affecting the separation and discharge of waste such as cell debris. On the other hand, the magnetic force is too strong, which can easily damage the magnetic beads 14 and the sample tube 7.

[0026] The top of the sample tube 7 is threaded with a cover 6. The top of the body 1 is fixed with a waste liquid extraction seat 4. A waste liquid collection tank 12 is installed on one side of the body 1. A drain pipe 11 is provided between the waste liquid collection tank 12 and the waste liquid extraction seat 4. A corrosion-resistant pump 13 is installed on one side of the waste liquid collection tank 12.

[0027] The cover 6 is fixed with a connecting pipe 5 that extends vertically to the bottom of the sample tube 7. Both ends of the bottom of the waste liquid extraction seat 4 are provided with a matching interface 3 that matches the connecting pipe 5.

[0028] Both the connecting pipe 5 and the connecting interface 3 are provided with a rubber sealing layer;

[0029] Specifically, such as Figure 1 , Figure 2 and Figure 5As shown, the user can use the sliding connection formed by the positioning slider 18 and the positioning rail 19 to slide the integrated carrier 20, which contains two rows of sample tubes 7 containing dairy cow gene samples and magnetic beads 14, into the machine body 1. Then, the two telescopic cylinders 9 are activated, and their output ends extend and press the positioning slider 18 inside the positioning rail 19 to achieve pressing and locking, which improves stability. After the integrated carrier 20 is assembled into the machine body 1, the connecting pipe 5 at the top of the sample tube 7 will be aligned with the connecting interface 3 on the waste liquid extraction seat 4.

[0030] Both ends of the body 1 are equipped with ultraviolet sterilization lamps 2, which facilitates ultraviolet sterilization of the internal environment of the body 1.

[0031] The top of the integrated shelf 20 is evenly provided with insertion slots that match the sample tubes 7.

[0032] Telescopic cylinders 9 are installed at both ends of the body 1, and the output end of the telescopic cylinders 9 extends laterally into the interior of the positioning slide rail 19.

[0033] The bottom of both ends of the integrated shelf 20 is provided with positioning slide bars 18 that match the positioning slide rail 19;

[0034] The inner wall of the body 1 is coated with a nano-copper antibacterial coating, and the waste liquid collection tank 12 is equipped with a filter layer, which enhances the antibacterial effect of the inner wall of the body 1.

[0035] Working principle: When in use, with an external power supply, the user first uses the sliding connection formed by the positioning slider 18 and the positioning rail 19 to slide the integrated carrier 20, which contains two rows of sample tubes 7 containing dairy cow gene samples and magnetic beads 14, into the machine body 1. Then, two telescopic cylinders 9 are activated, and their output ends extend and press against the positioning slider 18 inside the positioning rail 19 to achieve clamping and locking, improving stability. After the integrated carrier 20 is assembled into the machine body 1, the connecting pipe 5 at the top of the sample tube 7 will be aligned with the connecting interface 3 on the waste liquid extraction seat 4, facilitating subsequent waste liquid extraction and separation operations. Furthermore, the user operates the PLC controller 10 to control the current intensity of the electromagnet 17 installed inside the magnetic frame 8 through the programmable power supply 16. The magnetic field strength will change linearly with the current, that is, the greater the current, the stronger the magnetic field. In addition, the integrated carrier 20 is installed with the magnetic beads 14. The Hall effect sensor 21 in the magnetic field is based on the Hall effect. When current passes through a semiconductor wafer and it is in a magnetic field, a Hall voltage proportional to the magnetic field strength is generated on both sides of the wafer. By measuring this voltage through the Hall effect sensor 21, the magnetic field strength can be calculated by the PLC controller 10. This allows the PLC controller 10 to intelligently control the magnetic field strength, optimizing the effect of attracting the magnetic beads 14 carrying nucleic acids. It avoids the magnetic beads 14 not being completely attracted due to insufficient magnetic force, which would affect the separation and discharge of waste such as cell debris. On the other hand, excessive magnetic force can easily damage the magnetic beads 14 and the sample tube 7. Finally, after the magnetic beads 14 carrying nucleic acids are attracted and fixed inside the sample tube 7, the corrosion-resistant pump 13 is started. The waste inside the sample tube 7 can be automatically emptied through the drain pipe 11 and the waste liquid extraction seat 4. This can realize the automated and efficient separation of the bovine genome.

[0036] It will be apparent to those skilled in the art that this invention is not limited to the details of the exemplary embodiments described above, and that it can be implemented in other specific forms without departing from the spirit or essential characteristics of this invention. Therefore, the embodiments should be considered illustrative and non-limiting in all respects, and the scope of this invention is defined by the appended claims rather than the foregoing description. Thus, it is intended that all variations falling within the meaning and scope of equivalents of the claims be included within this invention. No reference numerals in the claims should be construed as limiting the scope of the claims.

Claims

1. A bovine genome magnetic bead separation device, characterized in that, The system includes a body (1), on which a PLC controller (10) is installed on the outer wall. A magnetic frame (8) is fixed at the bottom inside the body (1). Electromagnets (17) are evenly installed inside the magnetic frame (8). A shielded housing (15) is fixed inside the body (1) on one side of the magnetic frame (8). A programmable power supply (16) is installed inside the shielded housing (15). A Hall effect sensor (21) is installed inside the body (1) above the magnetic frame (8). Positioning slide rails (19) are fixed at both ends inside the magnetic frame (8). Adjacent positioning slide rails are fixed at both ends. An integrated rack (20) is slidably connected between the slide rails (19). Sample tubes (7) are evenly mounted on the integrated rack (20). Magnetic beads (14) are evenly placed inside the sample tubes (7). A cap (6) is threaded to the top of the sample tubes (7). A waste liquid extraction seat (4) is fixed at the top inside the body (1). A waste liquid collection box (12) is installed on one side of the body (1). A drain pipe (11) is provided between the waste liquid collection box (12) and the waste liquid extraction seat (4). A corrosion-resistant pump (13) is installed on one side of the waste liquid collection box (12).

2. The bovine genome magnetic bead separation device according to claim 1, characterized in that: The cover (6) is fixed with a connecting pipe fitting (5) that extends vertically to the bottom of the sample tube (7). Both ends of the bottom of the waste liquid extraction seat (4) are provided with a matching interface (3) that matches the connecting pipe fitting (5).

3. The bovine genome magnetic bead separation device according to claim 2, characterized in that: Both the connecting pipe fitting (5) and the connecting interface (3) are provided with a rubber sealing layer.

4. The bovine genome magnetic bead separation device according to claim 1, characterized in that: Both ends of the body (1) are equipped with ultraviolet sterilization lamps (2).

5. The bovine genome magnetic bead separation device according to claim 1, characterized in that: The top of the integrated carrier (20) is uniformly provided with insertion and placement ports that match the sample tubes (7).

6. The bovine genome magnetic bead separation device according to claim 1, characterized in that: Both ends of the body (1) are equipped with telescopic cylinders (9), and the output end of the telescopic cylinders (9) extends laterally into the interior of the positioning slide rail (19).

7. The bovine genome magnetic bead separation device according to claim 1, characterized in that: The bottom of both ends of the integrated shelf (20) is provided with positioning slides (18) that match the positioning slide rail (19).

8. The bovine genome magnetic bead separation device according to claim 1, characterized in that: The inner wall of the body (1) is provided with a nano copper antibacterial coating, and the inside of the waste liquid collection box (12) is provided with a filter layer.

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