A continuous reaction apparatus for producing l-asparagine from fumaric acid

Abstract

The utility model discloses a kind of fumaric acid production L-Asparagine's continuous reaction equipment, belong to biological preparation equipment technical field.Adopt double-layer design, internal reaction warehouse reserves reaction liquid, external heat preservation warehouse carries out heat preservation and drainage, the rotating device of reaction warehouse bottom combines centrifugal separation with filtration separation, through high-speed rotation reaction warehouse with filter hole, reaction liquid is discharged quickly, retains immobilized cell, can filter out reaction liquid rapidly and carry out new round of reaction after short time reaction end, overall structure is simple, flexible operation, strong practicality;And greatly alleviate the problem of filter hole blockage.

Description

Technical Field

[0001] This invention relates to a continuous reaction apparatus for the production of L-asparagine from fumaric acid, belonging to the field of bio-preparation equipment technology. Background Technology

[0002] L-Asparagine is an optical isomer of asparagine and is widely used in food, pharmaceuticals, chemical synthesis, and microbial culture. In the food industry, it can be used as a food additive in soft drinks. When heated with sugar, it undergoes a Maillard reaction (amino-carbonyl reaction) to produce unique aromatic compounds, which are used to make refreshing beverages. In the pharmaceutical field, it can be used in amino acid infusions and has functions such as lowering blood pressure, relieving asthma, and treating peptic ulcers and gastric dysfunction. It can also be used to treat myocardial infarction, myocardial metabolic disorders, heart failure, cardiac conduction block, and fatigue syndrome. In addition, asparagine is crucial for brain development and function and plays a key role in cancer treatment and protein glycosylation.

[0003] Currently, asparagine is prepared from fumarate using a three-enzyme cascade consisting of aspartate aminopyrate lyase, asparagine synthase, and polyphosphokinase. However, during the preparation process, the high catalytic efficiency of asparagine synthase and the synergistic effect of the three enzymes result in a high product yield in a short time. But when L-asparagine accumulates to a certain level, it can inhibit asparagine synthase through feedback, slowing down the enzymatic reaction. Furthermore, the generated L-asparagine may rebind to the enzyme's active site, hindering the normal binding of the substrate and enzyme, further inhibiting enzyme activity, leading to reduced substrate conversion and increased difficulty in product separation and purification. Therefore, it is impossible to use a closed reactor to prepare L-asparagine in large quantities in a single operation.

[0004] Currently, the most commonly used continuous reactor in existing technology is the continuous stirred tank reactor. In this reactor, raw materials and reactants are continuously and stably fed into the reactor through a feed pipe. The reactants are then continuously stirred by a stirrer in the reactor to ensure thorough mixing. The reaction products flow out through a discharge pipe, thus achieving a continuous reaction. Although this reactor has a high cell loading capacity, is simple to operate, and is easy to control, it suffers from time-consuming and high-loss issues in the natural sedimentation and centrifugal separation and recovery of small-particle diatomaceous earth. Furthermore, filtration separation suffers from filter pore clogging.

[0005] To address the problems existing in the prior art, Raffaella R. et al., in their work "Production of arabitol from glycerol by immobilized cells of Wickerhamomyces anomalus", proposed a new approach. The WC1501 series utilizes stirred tank reactors (STR), packed bed reactors (PBR), fluidized bed reactors (FBR), and airlift reactors (ALR) to produce arabinitol from glycerol using immobilized cells. STR is suitable for reactions with low stirring speeds and low oxygen requirements. However, in STR, reactants and catalysts are mixed during stirring, and the catalyst undergoes physical or chemical changes during the reaction, making it difficult to achieve automated catalyst separation to allow the reaction to continue. While PBR and FBR solve the problem of rapid catalyst separation, the reaction suffers from uneven reactant distribution due to weak diffusion and poor mass transfer, resulting in a low reaction rate. ALR uses lift generated by the gas-liquid density difference to circulate the fluid, increasing fluid flow and mixing, significantly alleviating mass transfer problems compared to PBR and FBR. However, it may suffer from uneven gas-liquid distribution leading to uneven substrate mixing inside the reactor, limited adjustable range of operating parameters due to gas-liquid flow characteristics, and poor adaptability to fermentation broths with high viscosity. In their paper "Development and operation of immobilized cell plug flow bioreactor (PFR) for treatment of textile industry effluent and evaluation of its working efficiency," Koundal, S. et al. proposed an immobilized cell plug flow bioreactor (PFR) for treating textile industry wastewater. PFR is an ideal continuous flow reactor, whose core assumption is that the fluid is propelled in the reactor in the form of a plug flow, that is, fluid particles pass through the reactor sequentially along the axial direction without backmixing. However, it has problems such as high cost, difficulty in scaling up, and easy clogging of the filter membrane.

[0006] Therefore, there is an urgent need to design a continuous reaction device that can easily separate immobilized cells and liquids while ensuring good mass transfer. Utility Model Content

[0007] To achieve the above objectives, the present invention is implemented through the following technical solution: a continuous reaction device for preparing L-asparagine from fumaric acid, comprising a reaction device, a temperature control device, a stirring device, a rotating device, a draining device, and a fixing device;

[0008] The reaction device includes an insulated chamber, an insulated cover, a reaction chamber, a reaction cover, a filter hole, and a feeding port; the temperature control device includes a thermometer, a temperature control display, and a temperature control jacket; the stirring device includes a central shaft, a motor, a motor bracket, and fan blades; the rotating device includes an electric motor, a reduction clutch, a bracket, a balance block, a rotating shaft, and a rotating chassis; the draining device includes a drain pipe and a baffle plate; and the fixing device includes a fixing bracket and a base.

[0009] In the reaction apparatus and fixing device: the heat preservation chamber is fixed to the base by a fixing bracket, the reaction chamber is located inside the heat preservation chamber and does not contact the heat preservation chamber, and several filter holes are evenly arranged on the wall of the reaction chamber; the heat preservation cover and the reaction cover are respectively set on the top of the heat preservation chamber and the reaction chamber and have through holes at the same position in the vertical direction; the feed port is set on the heat preservation chamber and extends inward to the reaction chamber, penetrating the heat preservation chamber and the reaction chamber;

[0010] In the temperature control device: the temperature control jacket is set on the inner wall of the heat preservation chamber, one end of the thermometer extends into the reaction chamber, and the other end extends out of the heat preservation chamber and is connected to the temperature control display.

[0011] In the stirring device: the motor is fixed to the through hole position on the top of the heat preservation cover by the motor bracket. One end of the central shaft is fixedly connected to the motor, and the other end extends into the reaction chamber through the through hole. Several sets of fan blades are set on the central shaft at the end that extends into the reaction chamber.

[0012] In the rotating device: the rotating shaft is located at the center of the bottom of the reaction chamber, and the rotating chassis is fixedly connected to the rotating shaft; the support is located at the bottom of the reaction chamber and fixedly connected to the rotating shaft, and its width is equal to that of the reaction chamber; the reduction clutch is located on the other side of the support at the position corresponding to the rotating shaft; the motor and the balance block are respectively located on both sides of the reduction clutch.

[0013] In the drainage device: drainage pipes are set on both sides of the bottom of the heat preservation chamber, and baffles are set on both sides of the support in the rotating device. The length of the baffles is equal to the distance between the heat preservation chamber and the reaction chamber, and the baffles can be extended and retracted.

[0014] In one embodiment of this utility model, the temperature in the reaction chamber is monitored in real time through a temperature control display.

[0015] In one embodiment of this utility model, the temperature control display compares the real-time temperature in the reaction chamber with a preset temperature, and sends a control command to the temperature control jacket based on the comparison result.

[0016] In one embodiment of this utility model, the real-time temperature inside the reaction chamber is adjusted by a temperature control jacket;

[0017] In one embodiment of this invention, the rotation of the fan blades ensures uniform mixing of cells and substrates in the reaction chamber, preventing precipitation.

[0018] In one embodiment of this utility model, a balance block is set to ensure the stable operation of the rotating device;

[0019] In one embodiment of this utility model, the pore size of the filter can be set according to the actual use to ensure the separation of immobilized cells and reaction solution during centrifugation.

[0020] In one embodiment of this invention, the separation of liquid and cells in the reaction chamber is achieved through filter holes;

[0021] In one embodiment of this utility model, drainage is achieved through a retractable baffle and a drainage pipe;

[0022] In one embodiment of this utility model, the rotating shaft drives the rotating chassis to rotate, thereby driving the reaction chamber to rotate. The rotating chassis buffers the force between the rotating shaft and the reaction chamber, preventing the rotating shaft from breaking.

[0023] In one embodiment of this utility model, the reaction chamber is fixedly connected to the rotating chassis, and the rotation of the reaction chamber is achieved by rotating the chassis.

[0024] In one embodiment of this utility model, filter holes are provided on the rotating chassis to ensure that the liquid located below the rotating chassis during centrifugation can also be completely removed.

[0025] Advantages of this utility model:

[0026] (1) This utility model provides a continuous reaction equipment for the production of L-asparagine from fumaric acid. By combining the temperature control device, stirring device, rotating device and draining device, the reaction liquid can be quickly filtered out after a short reaction and a new round of reaction can be carried out. The overall structure is simple, the operation is flexible and the practicality is strong.

[0027] (2) This utility model provides a continuous reaction device for the production of L-asparagine from fumaric acid. The centrifugal separation and filtration separation are combined by the rotating device at the bottom of the reaction chamber. The reaction liquid is quickly discharged by the high-speed rotating reaction chamber with filter holes, and the immobilized cells are retained. At the same time, the problem of filter hole clogging is greatly reduced. Attached Figure Description

[0028] Figure 1 A schematic diagram of the overall structure of a continuous reaction apparatus for producing L-asparagine from fumaric acid, provided by this utility model;

[0029] Figure 2A schematic diagram of a temperature control device in a continuous reaction apparatus for producing L-asparagine from fumaric acid, provided by this utility model;

[0030] Figure 3 A schematic diagram of the stirring device in a continuous reaction apparatus for producing L-asparagine from fumaric acid, provided by this utility model;

[0031] Figure 4 A schematic diagram of the rotating device in a continuous reaction apparatus for producing L-asparagine from fumaric acid, provided by this utility model;

[0032] Figure 5 A schematic diagram of the draining device in a continuous reaction apparatus for producing L-asparagine from fumaric acid, provided by this utility model;

[0033] In the diagram, the components are: electric motor-1, reduction clutch-2, bracket-3, central shaft-4, motor-5, balance block-6, fan blade-7, thermometer-8, filter hole-9, drain pipe-10, base-11, fixed bracket-12, feed inlet-13, rotating shaft-14, reaction chamber-15, heat preservation chamber-16, temperature control display-17, temperature control jacket-18, baffle plate-19, heat preservation cover-20, reaction cover-21, motor bracket-22, and rotating chassis-23. Detailed Implementation

[0034] The technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0035] Example 1

[0036] This embodiment provides a continuous reaction apparatus for the production of L-asparagine from fumaric acid. The overall structure of the apparatus is as follows: Figure 1 As shown, it includes a reaction apparatus, a temperature control apparatus, a stirring apparatus, a rotating apparatus, a draining apparatus, and a fixing apparatus.

[0037] The reaction device includes an insulated chamber 16, an insulated cover 20, a reaction chamber 15, a reaction cover 21, a filter hole 9, and a feed inlet 13; the temperature control device includes a thermometer 8, a temperature control display 17, and a temperature control jacket 18; the stirring device includes a central shaft 4, a motor 5, a motor bracket 22, and a fan blade 7; the rotating device includes a motor 1, a reduction clutch 2, a bracket 3, a balance block 6, a rotating shaft 14, and a rotating chassis 23; the draining device includes a drain pipe 10 and a baffle plate 19; and the fixing device includes a fixing bracket 12 and a base 11.

[0038] In the reaction apparatus and fixing device: the heat preservation chamber 16 is fixed to the base by a fixing bracket, the reaction chamber 15 is located inside the heat preservation chamber 16 and does not contact the heat preservation chamber 16, and a number of filter holes 9 are evenly arranged on the side wall of the reaction chamber 15; the heat preservation cover 20 and the reaction cover 21 are respectively set on the top of the heat preservation chamber 16 and the reaction chamber 15 and are provided with through holes at the same position in the vertical direction; the feed port 13 is set on the heat preservation chamber 16 and extends inward to the reaction chamber 15, penetrating the heat preservation chamber 16 and the reaction chamber 15;

[0039] The structure of the temperature control device is as follows Figure 2 As shown; the temperature control jacket is set on the inner wall of the heat preservation chamber 16, one end of the thermometer 8 extends into the reaction chamber 15, and the other end extends out of the heat preservation chamber 16 and is connected to the temperature control display 17.

[0040] The structure of the stirring device is as follows Figure 3 As shown; the motor 5 is fixed to the through hole position on the top of the heat preservation cover 20 through the motor bracket 22. One end of the central shaft 4 is fixedly connected to the motor 5, and the other end extends into the interior of the reaction chamber 15 through the through hole. Several sets of fan blades 7 are set on the central shaft 4 that extends into the interior of the reaction chamber 15.

[0041] The structure of the rotating device is as follows Figure 4 As shown; the support 3 is set at the bottom of the reaction chamber 15 and is equal in width to the reaction chamber 15. The deceleration clutch 2 is fixedly connected to the center position below the support 3. The motor 1 is set on one side of the deceleration clutch 2 and the balance block is set on the other side. The distance between the motor 1 and the balance block 6 and the deceleration clutch 2 is equal. The rotating shaft 14 is set on the support 3 at the position corresponding to the deceleration clutch 2, and the rotating chassis 23 is set around the rotating shaft 14.

[0042] The structure of the drainage device is as follows Figure 5 As shown; the drain pipe 10 is set on both sides of the bottom of the heat preservation chamber 16, and the baffle plate 19 is set on both sides of the bracket 3 in the rotating device. The length of the baffle plate 19 is equal to the distance between the heat preservation chamber 16 and the reaction chamber 15, and the baffle plate 19 can be extended and retracted.

[0043] The temperature control display 17 in the temperature control device monitors the temperature in the reaction chamber 15 in real time and compares the real-time temperature with the preset temperature. Based on the comparison result, it sends a control command to the temperature control jacket 18. The temperature control jacket 18 adjusts the temperature in the reaction chamber 15 in real time according to the command.

[0044] The stirring device enables uniform mixing of cells and substrates in reaction chamber 15, preventing sedimentation and improving production efficiency.

[0045] The balance block 6 in the rotating device ensures the stable operation of the rotating device; the rotating shaft 14 drives the rotating base 23 to rotate, thereby driving the reaction chamber 15 to rotate. The rotating base 23 buffers the force between the rotating shaft 14 and the reaction chamber 15 to prevent the rotating shaft 14 from breaking; both the reaction chamber 15 and the rotating base 23 are provided with filter holes 9. The pore size of the filter holes 9 can be set according to the actual use to ensure the separation of immobilized cells from the reaction solution during centrifugation; the baffle plate 19 in the drainage device drains liquid by contraction.

[0046] This equipment enables a continuous reaction for the production of L-asparagine from fumaric acid.

[0047] The working principle of this utility model:

[0048] The continuous reaction equipment consists of an inner reaction chamber 15 and an outer insulation chamber 16, which are connected to the base 11 by a fixed bracket 12. After the reaction substrate is added to the reaction chamber 15 through the feeding port 13, the reaction temperature is set on the temperature control display 17 and the temperature control device is turned on. The temperature in the reaction chamber 15 is monitored in real time by the thermometer 8 and the temperature control display 17, and the temperature of the temperature control jacket 18 is adjusted by the temperature control display 17 to regulate the internal temperature of the reaction chamber 15.

[0049] After setting the temperature, the stirring device is turned on. The motor 5, fixed on the motor bracket 22, drives the central shaft 4 to rotate, which in turn drives the fan blades 7 to rotate, so that the reaction substrate in the reaction chamber 15 is mixed evenly. After one batch of reaction is completed, the baffle plate 19 is retracted and the rotating device is turned on. The motor 1 drives the reduction clutch 2, the rotating shaft 14 and the rotating chassis 23 to rotate, and the balance block 6 ensures the stable operation of the rotating device. The rotating chassis 23 drives the reaction chamber 15 to rotate, so that the immobilized cells in the reaction chamber 15 settle down. Under the action of centrifugal force, the reaction liquid passes through the side wall of the reaction chamber 15 and the filter holes 9 in the rotating chassis 23, flows out through the drain pipe 10 and is collected. After the draining is completed, the baffle plate 19 is extended to block the drain pipe 10 and the substrate is re-injected for the next batch of reaction.

[0050] The components of this invention, including the electric motor 1, reduction clutch 2, bracket 3, central shaft 4, motor 5, balance block 6, fan blade 7, thermometer 8, filter hole 9, drain pipe 10, base 11, fixed bracket 12, feed port 13, rotating shaft 14, reaction chamber 15, heat preservation chamber 16, temperature control display 17, temperature control jacket 18, baffle 19, heat preservation cover 20, reaction cover 21, motor bracket 22, and rotating chassis 23, are all general standard parts or parts known to those skilled in the art. Their structure and principle can be learned by those skilled in the art through technical manuals or conventional experimental methods.

[0051] The foregoing has shown and described the basic principles, main features, and advantages of the present invention. For those skilled in the art, the present invention is not limited to the details of the exemplary embodiments described above, and can be implemented in other specific forms without departing from the spirit or basic characteristics of the invention. Therefore, the embodiments should be considered illustrative and non-limiting. The scope of the invention is defined by the appended claims rather than the foregoing description, and thus all variations falling within the meaning and scope of equivalents of the claims are intended to be included within the present invention. No reference numerals in the claims should be construed as limiting the scope of the claims.

Claims

1. A continuous reaction apparatus for producing L-asparagine from fumaric acid, characterized by, The device comprises a reaction device, a temperature control device, a stirring device, a rotating device, a liquid discharge device, and a fixing device; The reaction device is fixed by the fixing device, and the temperature control device, the stirring device, the rotating device, and the liquid discharge device are arranged at different positions on the reaction device; the temperature control device is used to realize real-time adjustment of the temperature of the reaction device; the stirring device is used to ensure uniform mixing of the reaction substrate in the reaction device; and the rotating device and the liquid discharge device are used to separate the immobilized cells from the reaction liquid in the reaction substrate; The reaction device comprises a reaction bin (15), a heat preservation bin (16), a feeding port (13), and filter holes (9); the temperature control device comprises a thermometer (8), a temperature control display (17), and a temperature control jacket (18); the stirring device comprises a central shaft (4), a fan blade (7), a motor (5), and a motor support (22); the rotating device comprises a motor (1), a speed reducer clutch (2), a support (3), a balance block (6), a rotating shaft (14), and a rotating base (23); the liquid discharge device comprises a liquid discharge pipeline (10) and a water baffle (19); and the fixing device comprises a fixing support (12) and a base (11).

2. The apparatus of claim 1, wherein, In the reaction device and the fixing device, the heat preservation bin (16) is fixed on the base (11) by the fixing support (12), the reaction bin (15) is located inside the heat preservation bin (16) and does not contact the heat preservation bin (16), the top of the heat preservation bin (16) and the reaction bin (15) is respectively provided with a heat preservation cover (20) and a reaction cover (21), and a through hole is arranged at a same position in the vertical direction; the feeding port (13) is arranged on one side of the heat preservation cover (20) and communicates the heat preservation bin (16) and the reaction bin (15); and a plurality of filter holes (9) are uniformly arranged on the side wall of the reaction bin (15).

3. The apparatus of claim 2, wherein, In the temperature control device, the temperature control jacket (18) is arranged on the inner wall of the heat preservation bin (16), one end of the thermometer (8) extends from the top of the heat preservation bin (16) to the inside of the reaction bin (15), and the other end is connected to the temperature control display (17).

4. The apparatus of claim 3, wherein, In the stirring device, the motor (5) is fixed at the through hole of the heat preservation cover (20) by the motor support (22), one end of the central shaft (4) is fixedly connected to the motor (5), and the other end extends to the inside of the reaction bin (15) and is provided with a plurality of fan blades (7).

5. The apparatus of claim 4, wherein, In the rotating device, the rotating shaft (14) is arranged at the center position of the bottom of the reaction bin (15), the rotating base (23) is fixedly connected to the rotating shaft (14), the support (3) is arranged at the bottom of the reaction bin (15) and is fixedly connected to the rotating shaft (14) and has a width equal to that of the reaction bin (15), the speed reducer clutch (2) is arranged at a position corresponding to the rotating shaft (14) on the other side of the support (3), and the motor (1) and the balance block (6) are arranged on the two sides of the speed reducer clutch (2) and have equal distances.

6. The apparatus of claim 5, wherein, The liquid discharge device is characterized in that: the liquid discharge pipeline (10) is arranged at the two sides of the bottom of the heat preservation bin (16), the water baffle (19) is arranged at the two ends of the support (3), the length of the water baffle (19) is equal to the distance between the heat preservation bin (16) and the reaction bin (15), and the water baffle (19) can be telescoped between the one end of the support (3) and the side wall of the heat preservation bin (16).

7. The apparatus of claim 6, wherein, The temperature control display (17) can realize real-time adjustment of the temperature of the reaction bin (15) in the reaction process by adjusting the temperature of the temperature control jacket (18).

8. The apparatus of claim 7, wherein, The number of the fan blades (7) is set according to the actual use, so as to ensure that the reaction substrate in the reaction bin (15) is mixed uniformly.

9. The apparatus of claim 8, wherein, The rotating disc (23) buffers the acting force between the rotating shaft (14) and the reaction bin (15), so as to prevent the rotating shaft (14) from being broken.

10. The apparatus of claim 9, wherein, The filter holes (9) are arranged on the side wall of the reaction bin (15) and the rotating disc (23), and the aperture of the filter holes (9) is adjusted according to the actual use, so as to ensure that the cells and the reaction liquid are separated in the rotating centrifugal process.

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