Gradient screening device for functional strains based on distiller's solubles

Abstract

The utility model relates to the field of vinasse filtrate, specifically relates to a functional strain gradient screening device based on vinasse filtrate, including gradient screening base, the main part for carrying out strain gradient screening, screening jar, fixedly installed in the top of gradient screening base, for bearing vinasse filtrate, and the inside of screening jar all is equipped with fixed sliding slot. The utility model is provided with stainless steel screen, temperature regulator, baffle, circulating pump, atomization spraying head, multistage ceramic mould frame, gradient arrangement ceramic membrane, primary biological reaction bin and secondary biological reaction bin, when using the functional strain gradient screening device based on vinasse filtrate, vinasse filtrate is first screened coarsely through stainless steel screen, removes big granule residue, then baffle divides screening jar into two spaces, the vinasse filtrate after rough filtration is in the upper space of baffle, is affected by the adjustment of external temperature regulator, adjusts filtrate to target bacteria suitable condition.

Description

Technical Field

[0001] This utility model relates to the field of distiller's grains filtrate, specifically to a functional strain gradient screening device based on distiller's grains filtrate. Background Technology

[0002] Distillers' grains filtrate is a byproduct of the brewing process. Specifically, it refers to the liquid portion obtained by filtration after starchy grains or tubers have undergone saccharification, fermentation, and distillation to extract alcohol. The functional strain gradient screening device for distillers' grains filtrate is a device specifically designed to screen for microbial strains with specific functions from the filtrate. This device simulates different environmental conditions to achieve stepwise screening and enrichment of strains, thereby identifying microbial strains with target functions, improving the screening efficiency of microbial strains, and obtaining strains with efficient degradation, fermentation, or enzyme production capabilities. This provides high-quality microbial resources for fields such as industrial biotechnology, environmental protection, and agriculture.

[0003] Existing functional strain gradient screening devices based on distiller's grains filtrate have limitations during use. Due to the complex microbial community in distiller's grains filtrate and the low abundance of target strains, conventional plate screening is time-consuming and has low throughput. Furthermore, steps such as centrifugation and chemical treatment can easily damage the activity of strains. It is difficult to dynamically enrich the target strains in the early stages of screening by taking into account their metabolic characteristics (such as specific substrate utilization and tolerance). In the long run, strain loss or cross-contamination can also occur due to the separation of physical filtration and biological reaction in traditional devices.

[0004] Therefore, it is necessary to invent a functional strain gradient screening device based on distiller's grains filtrate to solve the above problems. Utility Model Content

[0005] The purpose of this invention is to provide a gradient screening device for functional strains based on distiller's grains filtrate. Through a stainless steel screen, temperature regulator, partition plate, circulating pump, atomizing spray head, multi-stage ceramic frame, gradient-arranged ceramic membrane, primary bioreactor, and secondary bioreactor, the device achieves improved screening efficiency, shortens the target bacteria enrichment cycle by 50%–70%, and maintains a strain survival rate >90%. Furthermore, it possesses high specificity, allowing simultaneous screening of multiple functional bacteria without interference. The target bacteria percentage can be increased from an initial 0.1% to a final >30%. With long-term use, this device solves the problem of efficient and targeted screening of high-value-added functional strains in distiller's grains filtrate. This study addresses the issues of selection and enrichment by integrating physical separation and biological culture techniques. It overcomes the problems of low efficiency, cumbersome procedures, and easy loss of target strains in traditional screening methods. This solves the problems of existing functional strain gradient screening devices based on distiller's grains filtrate. Due to the complex microbial community in distiller's grains filtrate and the low abundance of target strains, conventional plate screening is time-consuming and has low throughput. Moreover, steps such as centrifugation and chemical treatment can easily damage the activity of strains, making it difficult to dynamically enrich the target bacteria in the early stages of screening based on their metabolic characteristics. Furthermore, long-term use can lead to strain loss or cross-contamination due to the physical filtration and biological reaction separation in traditional devices.

[0006] To achieve the above objectives, this utility model provides the following technical solution: a functional strain gradient screening device based on distiller's grains filtrate, comprising a gradient screening base and a main body for performing strain gradient screening;

[0007] The screening tank is fixedly installed above the gradient screening base to carry the filtrate from the distiller's grains. The interior of the screening tank is provided with a fixed sliding groove, and a fixed slider is slidably connected inside the fixed sliding groove. A stainless steel screen is fixedly installed on the outside of the fixed slider.

[0008] A temperature regulator is fixedly installed on the outside of the screening tank to regulate the filtrate. A partition plate is fixedly installed inside the screening tank. A circulation pump is fixedly installed on the outside of the screening tank. The output end of the circulation pump is fixedly connected to a spray chamber. Atomizing spray heads are fixedly installed inside the spray chamber. A multi-stage ceramic mold frame is fixedly installed inside the screening tank. Gradiently arranged ceramic membranes are fixedly installed inside the multi-stage ceramic mold frame.

[0009] A support frame is fixedly installed above the gradient screening base. A primary bioreactor chamber is fixedly installed inside the support frame. An environmental controller is fixedly installed outside the primary bioreactor chamber. An online monitoring probe is fixedly installed inside each primary bioreactor chamber.

[0010] An electromagnetic control valve is fixedly installed at the bottom of the primary bioreactor chamber to control the unidirectional flow of fluid. The bottom end of the electromagnetic control valve is fixedly connected to a secondary bioreactor chamber. A secondary membrane filter frame is fixedly installed inside the secondary bioreactor chamber, and a discharge pipe is fixedly installed outside the secondary bioreactor chamber.

[0011] Preferably, a tank cover is fixedly installed on the top of the screening tank, and a feed inlet is provided on the top of the tank cover.

[0012] Preferably, the spraying chamber is fixedly connected to the interior of the screening tank, and the atomizing spray heads are arranged in a ring array on the spraying chamber.

[0013] Preferably, a backwash water tank is fixedly installed on the outside of the screening tank, a pulse water pump is fixedly installed below the backwash water tank, a delivery pipe is fixedly installed at the output end of the pulse water pump, and the other end of the delivery pipe is fixedly connected to the outside of the spraying chamber.

[0014] Preferably, pressure sensors are fixedly installed above and below the multi-stage ceramic mold frame, and a drain pipe is fixedly installed at the bottom of the screening tank.

[0015] Preferably, a feed pump is fixedly installed on the top of the support frame, and a feed pipe is fixedly installed on the output end of the feed pump. The other end of the feed pipe is fixedly connected to the bottom of the screening tank, and a PLC human-machine interface is fixedly installed on the outside of the support frame.

[0016] The technical effects and advantages provided by this utility model in the above technical solution are as follows:

[0017] This invention comprises a stainless steel screen, a temperature regulator, a partition plate, a circulating pump, an atomizing spray head, a multi-stage ceramic frame, a gradient-arranged ceramic membrane, a primary bioreactor, and a secondary bioreactor. When using this functional strain gradient screening device based on distiller's grains filtrate, the distiller's grains filtrate is first coarsely screened through the stainless steel screen to remove large particles. Then, the partition plate divides the screening tank into upper and lower spaces. The coarsely filtered distiller's grains filtrate in the upper space of the partition plate is adjusted to suitable conditions for the target bacteria by the external temperature regulator. Next, the temperature-adjusted distiller's grains filtrate is pumped out by the circulating pump and sprayed above the multi-stage ceramic frame and the gradient-arranged ceramic membrane. Because the multi-stage ceramic frame and the gradient-arranged ceramic membrane are arranged according to a pore size gradient, different sizes of bacteria / metabolites are retained at each stage. The filtered distiller's grains filtrate is then sent back to the primary bioreactor. Substrate can be added at the bottom of the primary bioreactor according to the needs of the target bacteria. Furthermore, external... The PLC human-machine interface allows operators to easily control the environmental controller and online monitoring probes in the primary bioreactor chamber for independent regulation of dissolved oxygen, temperature, and pH, as well as real-time feedback on OD600, DO, and pH. The filtrate from the primary bioreactor chamber then enters the secondary bioreactor chamber below, where it undergoes further filtration by a secondary membrane filter. Finally, the high-purity functional bacterial solution is output through the discharge pipe. This combination improves the device's screening efficiency, shortens the target bacteria enrichment cycle by 50%–70%, and achieves a bacterial survival rate >90%. It also exhibits high specificity, allowing simultaneous screening of multiple functional bacteria without interference. The target bacteria percentage can be increased from an initial 0.1% to a final >30%. Long-term use of this technology solves the problem of efficient targeted screening and enrichment of high-value-added functional bacteria in the filtrate. By integrating physical separation and biological culture technologies, it overcomes the problems of low efficiency, cumbersome procedures, and easy loss of target bacteria associated with traditional screening methods. Attached Figure Description

[0018] To more clearly illustrate the technical solutions in the embodiments of this application or the prior art, the drawings used in the embodiments will be briefly introduced below. Obviously, the drawings described below are only some embodiments recorded in this invention. For those skilled in the art, other drawings can be obtained based on these drawings.

[0019] Figure 1 This is a schematic diagram of the overall structure of this utility model;

[0020] Figure 2 This is a schematic diagram of the stainless steel screen structure of this utility model;

[0021] Figure 3 This is a schematic diagram of the circulating pump structure of this utility model;

[0022] Figure 4This is a schematic diagram of the material conveying pipe structure of this utility model;

[0023] Figure 5 This is a schematic diagram of the structure of the primary bioreactor chamber of this utility model;

[0024] Figure 6 This is a schematic diagram of the secondary bioreactor of this utility model.

[0025] In the diagram: 1. Gradient screening base; 2. Screening tank; 3. Fixed chute; 4. Fixed slider; 5. Stainless steel screen; 6. Tank cover; 7. Feed inlet; 8. Temperature regulator; 9. Baffle plate; 10. Circulation pump; 11. Spray chamber; 12. Atomizing spray head; 13. Backwash water tank; 14. Pulse water pump; 15. Delivery pipe; 16. Multi-stage ceramic mold frame; 17. Gradiently arranged ceramic membrane; 18. Pressure sensor; 19. Drain pipe; 20. Support frame; 21. Feed pump; 22. Feed pipe; 23. Primary bioreactor; 24. Environmental controller; 25. Online monitoring probe; 26. PLC human-machine interface; 27. Electromagnetic control valve; 28. Secondary bioreactor; 29. ​​Secondary membrane filter frame; 30. Discharge pipe. Detailed Implementation

[0026] To enable those skilled in the art to better understand the technical solution of this utility model, the present utility model will be further described in detail below with reference to the accompanying drawings.

[0027] according to Figures 1 to 6 As shown, a functional strain gradient screening device based on distiller's grains filtrate includes a gradient screening base 1, which is the main body for performing strain gradient screening.

[0028] Screening tank 2 is fixedly installed above gradient screening base 1 to carry distiller's grains filtrate. The interior of screening tank 2 is provided with fixed sliding grooves 3. Fixed sliders 4 are slidably connected inside the fixed sliding grooves 3. Stainless steel screens 5 are fixedly installed on the outside of the fixed sliders 4.

[0029] Temperature regulator 8 is fixedly installed on the outside of screening tank 2 for regulating filtrate. A partition plate 9 is fixedly installed inside screening tank 2. A circulation pump 10 is fixedly installed on the outside of screening tank 2. A spray chamber 11 is fixedly connected to the output end of circulation pump 10. Atomizing spray heads 12 are fixedly installed inside the spray chamber 11. A multi-stage ceramic mold frame 16 is fixedly installed inside screening tank 2. A gradient-arranged ceramic membrane 17 is fixedly installed inside the multi-stage ceramic mold frame 16.

[0030] The support frame 20 is fixedly installed above the gradient screening base 1. The primary bioreactor 23 is fixedly installed inside the support frame 20. An environmental controller 24 is fixedly installed outside the primary bioreactor 23. Online monitoring probes 25 are fixedly installed inside the primary bioreactor 23.

[0031] An electromagnetic control valve 27 is fixedly installed at the bottom of the primary bioreactor 23 to control the unidirectional flow of fluid. The bottom of the electromagnetic control valve 27 is fixedly connected to a secondary bioreactor 28. A secondary membrane filter frame 29 is fixedly installed inside the secondary bioreactor 28, and a discharge pipe 30 is fixedly installed outside the secondary bioreactor 28. The filtered distiller's grains filtrate is sent back to the primary bioreactor 23. Substrates such as cellulose and lactic acid can be added at the bottom of the primary bioreactor 23 according to the needs of the target bacteria. The external PLC human-machine interface 26 allows the operator to easily control the environmental controller 24 and online monitoring probe 25 in the primary bioreactor 23 for independent regulation of dissolved oxygen, temperature, pH, and real-time feedback on OD600, DO, pH, etc. After the reaction in the primary bioreactor 23, the distiller's grains filtrate enters the secondary bioreactor 28 below and is further filtered by the secondary membrane filter frame 29. Finally, the high-purity functional bacterial solution is output through the discharge pipe 30.

[0032] like Figure 1 , Figure 2 and Figure 3 As shown, a tank cover 6 is fixedly installed on the top of the screening tank 2, and a feed inlet 7 is provided above the tank cover 6. The feed inlet 7 is used to facilitate connection with external pipelines for batch feeding of lees filtrate into the screening tank 2. The spray chamber 11 is fixedly connected to the inside of the screening tank 2, and the atomizing spray heads 12 are arranged in a ring array on the spray chamber 11. The spray chamber 11 and the atomizing spray heads 12 are used to assist in uniformly spraying the lees filtrate drawn by the circulating pump 10 onto the multi-stage ceramic mold frame 16 and the gradient-arranged ceramic membrane 1. Above 7, to facilitate filtration, a backwash water tank 13 is fixedly installed on the outside of the screening tank 2, and a pulse water pump 14 is fixedly installed below the backwash water tank 13. A delivery pipe 15 is fixedly installed at the output end of the pulse water pump 14, and the other end of the delivery pipe 15 is fixedly connected to the outside of the spray chamber 11. The liquid in the backwash water tank 13 can be drawn out by the pulse water pump 14 at regular intervals to reverse the pulse cleaning of the multi-stage ceramic mold frame 16 and the gradient-arranged ceramic membrane 17 below, thereby reducing membrane fouling.

[0033] like Figure 1 , Figure 4 , Figure 5 and Figure 6As shown, pressure sensors 18 are fixedly installed above and below the multi-stage ceramic mold frame 16, and a drain pipe 19 is fixedly installed at the bottom of the screening tank 2. The pressure sensors 18 installed above and below the multi-stage ceramic mold frame 16 can detect the inflow and outflow pressure, control the transmembrane pressure (0.1-0.3 MPa) and flow rate, and prevent membrane blockage. A feed pump 21 is fixedly installed on the top of the support frame 20, and a feed pipe 22 is fixedly installed at the output end of the feed pump 21. The other end of the feed pipe 22 is fixedly connected to the bottom of the screening tank 2. A PLC human-machine interface 26 is fixedly installed on the outside of the support frame 20. The PLC human-machine interface 26 can dynamically adjust the filtration parameters (pressure, flow rate, reaction chamber conditions, substrate addition, and environmental parameters) based on the data from the pressure sensors 18 and the online monitoring probe 25.

[0034] The working principle of this practical system is as follows: First, connect the external power supply and connect it to the external pipeline through the feed inlet 7. Batch of distiller's grains filtrate is then fed into the screening tank 2. The filtrate first undergoes coarse screening through the stainless steel screen 5 to remove large particles of residue. Then, the partition plate 9 divides the screening tank 2 into upper and lower spaces. The coarsely filtered distiller's grains filtrate is in the upper space of the partition plate 9. Under the influence of the external temperature regulator 8, the filtrate is adjusted to suitable conditions for the target bacteria, such as pH 6.5–7.5 and 30℃. Next, the circulation pump 10 is turned on, and the filtrate is... The circulating pump 10 draws out the temperature-adjusted distiller's grains filtrate and inputs it into the spray chamber 11, then sprays it out from the atomizing spray head 12, so that the distiller's grains filtrate is sprayed above the multi-stage ceramic mold 16 and the gradient-arranged ceramic membrane 17. Since the multi-stage ceramic mold 16 and the gradient-arranged ceramic membrane 17 are arranged according to the pore size gradient, such as 0.8μm→0.2μm→50kDa ultrafiltration, different sizes of bacteria and metabolites are retained step by step. Then the switch of the feed pump 21 at the top of the support frame 20 can be turned on, allowing the feed pump 21 to draw through the feed pipe 22. The filtered distiller's grains filtrate is then fed back into the primary bioreactor 23. Substrate such as cellulose or lactic acid can be added at the bottom of the primary bioreactor 23 to meet the needs of the target bacteria. An external PLC human-machine interface 26 allows operators to easily control the environmental controller 24 and online monitoring probe 25 within the primary bioreactor 23 for independent regulation of dissolved oxygen, temperature, and pH, and provides real-time feedback on OD600, DO, and pH. After the reaction in the primary bioreactor 23, the distiller's grains filtrate, under the control of an electromagnetic control valve 27, enters the secondary bioreactor 28 below, where it undergoes further filtration by a secondary membrane filter rack 29. The final high-purity functional bacterial solution is then output through the discharge pipe 30. This improves the device's screening efficiency, shortening the target bacteria enrichment cycle by 50%–70% (traditional 7 days → device 2 days), while maintaining a bacterial survival rate >90% compared to 60%–70% for centrifugation. Furthermore, it exhibits high specificity, allowing simultaneous screening of multiple functional bacteria, such as ester-producing yeast and cellulose-degrading bacteria, without interference. The target bacteria percentage can be increased from an initial 0.The concentration of high-value functional strains, such as enzyme-producing bacteria, probiotics, and flavor-synthesizing bacteria, in the distillers' grains filtrate has been increased from 1% to over 30% in the final product. This approach, with long-term use, also solves the problem of efficient targeted screening and enrichment of these strains. By integrating physical separation and biological culture technologies, it overcomes the problems of low efficiency, cumbersome procedures, and easy loss of target strains associated with traditional screening methods. Furthermore, the PLC human-machine interface 26 allows external operators to connect via the pressure sensor 18 and online monitoring probe 25, dynamically adjusting filtration parameters such as pressure, flow rate, reaction chamber conditions, substrate addition, and environmental parameters. When not in use, the pulse water pump 14 can periodically extract liquid from the backwash tank 13 to perform reverse pulse cleaning of the multi-stage ceramic mold 16 and the gradient-arranged ceramic membrane 17 below, thereby reducing membrane fouling. Finally, after completing the installation and use of the functional strain gradient screening device based on distillers' grains filtrate according to the above operations, turn off the circulation pump 10, the pulse water pump 14, and the feed pump 21. If not in use for a long period, simply disconnect the external power supply. This completes the usage process of the functional strain gradient screening device based on distillers' grains filtrate. .

[0035] The foregoing description only illustrates certain exemplary embodiments of the present invention. Undoubtedly, those skilled in the art can modify the described embodiments in various ways without departing from the spirit and scope of the present invention. Therefore, the above drawings and descriptions are illustrative in nature and should not be construed as limiting the scope of protection of the claims of the present invention.

Claims

1. A functional strain gradient screening device based on distiller's grains filtrate, characterized in that: include Gradient screening base (1), the main body used for gradient screening of strains; Screening tank (2) is fixedly installed above gradient screening base (1) for carrying distiller's grains filtrate. The screening tank (2) is provided with a fixed slide groove (3) inside. A fixed slider (4) is slidably connected inside the fixed slide groove (3). A stainless steel screen (5) is fixedly installed on the outside of the fixed slider (4). A temperature regulator (8) is fixedly installed on the outside of the screening tank (2) for regulating the filtrate. A partition plate (9) is fixedly installed inside the screening tank (2). A circulation pump (10) is fixedly installed on the outside of the screening tank (2). A spraying chamber (11) is fixedly connected to the output end of the circulation pump (10). Atomizing spray heads (12) are fixedly installed inside the spraying chamber (11). A multi-stage ceramic mold frame (16) is fixedly installed inside the screening tank (2). A gradient-arranged ceramic membrane (17) is fixedly installed inside the multi-stage ceramic mold frame (16). The support frame (20) is fixedly installed above the gradient screening base (1). The first-level bioreactor (23) is fixedly installed inside the support frame (20). An environmental controller (24) is fixedly installed outside the first-level bioreactor (23). An online monitoring probe (25) is fixedly installed inside the first-level bioreactor (23). An electromagnetic control valve (27) is fixedly installed at the bottom of the primary bioreactor (23) to control the unidirectional flow of fluid. The bottom end of the electromagnetic control valve (27) is fixedly connected to a secondary bioreactor (28). A secondary membrane filter frame (29) is fixedly installed inside the secondary bioreactor (28), and a discharge pipe (30) is fixedly installed outside the secondary bioreactor (28).

2. The functional strain gradient screening device based on distiller's grains filtrate according to claim 1, characterized in that: A can cover (6) is fixedly installed on the top of the screening tank (2), and a feed inlet (7) is provided on the top of the can cover (6).

3. The functional strain gradient screening device based on distiller's grains filtrate according to claim 1, characterized in that: The spraying chamber (11) is fixedly connected to the interior of the screening tank (2), and the atomizing spray heads (12) are arranged in a ring array on the spraying chamber (11).

4. The functional strain gradient screening device based on distiller's grains filtrate according to claim 1, characterized in that: A backwash water tank (13) is fixedly installed on the outside of the screening tank (2). A pulse water pump (14) is fixedly installed below the backwash water tank (13). A delivery pipe (15) is fixedly installed at the output end of the pulse water pump (14). The other end of the delivery pipe (15) is fixedly connected to the outside of the spraying chamber (11).

5. The functional strain gradient screening device based on distiller's grains filtrate according to claim 1, characterized in that: Pressure sensors (18) are fixedly installed above and below the multi-stage ceramic mold frame (16), and a drain pipe (19) is fixedly installed at the bottom of the screening tank (2).

6. The functional strain gradient screening device based on distiller's grains filtrate according to claim 1, characterized in that: A feed pump (21) is fixedly installed on the top of the support frame (20), and a feed pipe (22) is fixedly installed at the output end of the feed pump (21). The other end of the feed pipe (22) is fixedly connected to the bottom of the screening tank (2). A PLC human-machine interface (26) is fixedly installed on the outside of the support frame (20).

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