Flowing electric conversion fluid path and device

Abstract

The utility model provides a kind of flow type electric conversion liquid path and device, the flow type electric conversion liquid path includes: flow type electric conversion container, is placed in electrode clamp;Conveying pipeline, one end is communicated with the first end of flow type electric conversion container, the other end is communicated with the sample container for containing sample liquid;Collecting pipeline, one end is communicated with the second end of flow type electric conversion container, the other end is communicated with first collection container;Collecting pipeline or conveying pipeline is placed on drive pump;And air inlet pipeline, one end is communicated with conveying pipeline, the other end is communicated with atmosphere.The utility model improves the convenience of installation or replacement of flow type electric conversion liquid path, and also improves compatibility, and further improves electric conversion efficiency.

Description

Technical Field

[0001] This invention belongs to the field of cell processing technology, and in particular relates to a flow electroporation circuit and device. Background Technology

[0002] With the rapid development of medical technology, electroporation technology has been widely used and has become an important method for non-viral cell transfection. Existing electroporation devices can usually only achieve continuous flow, full-flow electroporation or intermittent flow, semi-flow electroporation, and cannot be compatible with both working states; moreover, the sample injection pipeline design of the electroporation device is complex, costly, and has poor installation convenience, thus resulting in low electroporation efficiency. Utility Model Content

[0003] This invention addresses the technical problems of poor compatibility and low efficiency of existing electro-conversion devices by providing a flow electro-conversion liquid circuit and device.

[0004] In view of the above technical problems, this utility model provides a flow electro-hydraulic circuit, including:

[0005] A flow electrophoresis container is placed in an electrode fixture;

[0006] The delivery pipeline is connected at one end to the first end of the flow electroporation container and at the other end to a sample container for holding the sample solution.

[0007] A collection pipeline, one end connected to the second end of the flow electrophoresis container, and the other end connected to the first collection container; the collection pipeline or the delivery pipeline is mounted on a drive pump; and

[0008] The air intake pipe is connected to the delivery pipe at one end and to the atmosphere at the other end.

[0009] Optionally, the delivery pipeline is placed on the first switching valve; the collection pipeline is placed on the second switching valve; the air inlet pipeline is placed on the third switching valve, one end of the air inlet pipeline is connected to the first connection point of the delivery pipeline, and the other end is connected to the atmosphere; the first connection point is located between the first switching valve and the flow electrostatic container, and the drive pump is located between the first connection point and the second switching valve.

[0010] Optionally, the flow electroporation circuit further includes a first sampling line placed on the fourth switching valve for sampling the sample solution before and / or after electroporation; the first sampling line is connected to a second connection point of the delivery line, the second connection point being located between the flow electroporation container and the first connection point; the drive pump is located between the second connection point and the second switching valve.

[0011] Optionally, the flow electro-hydraulic circuit further includes a sealing element disposed at the end of the first sampling line away from the delivery line and used to seal the first sampling line; or / and

[0012] The flow electro-hydraulic circuit further includes a first air filter disposed at the end of the first sampling line away from the delivery line; or / and

[0013] The flow electro-hydraulic circuit also includes a second collection container disposed at the end of the first sampling pipeline away from the delivery pipeline.

[0014] Optionally, the flow electro-hydraulic circuit further includes a second sampling line; the second sampling line is connected to a third connection point of the collection line, the third connection point being located between the first collection container and the second switching valve.

[0015] Optionally, the flow electroporation circuit further includes a sampling dropper disposed on the second sampling tube for holding the sample solution after electroporation; or / and

[0016] The flow electro-hydraulic circuit further includes a first clamp disposed on the second sampling line and used to control the on / off state of the second sampling line; or / and

[0017] The flow electro-hydraulic circuit also includes a second air filter disposed at the end of the second sampling line away from the collection line, and the second air filter is in communication with the atmosphere.

[0018] Optionally, the collection pipeline includes a first pipe section, a pump pipe section, and a second pipe section connected in sequence; the first pipe section is positioned on the second switch valve, the pump pipe section is positioned on the drive pump, the end of the first pipe section away from the pump pipe section is connected to the first collection container, and the end of the second pipe section away from the pump pipe section is connected to the flow electrostatic container; or / and

[0019] The flow electro-hydraulic circuit further includes at least one input line for inputting the liquid to be added; the input line is connected to a fourth connection point of the delivery line, the fourth connection point being located between the first switching valve and the flow electro-hydraulic container.

[0020] Optionally, the flow electro-hydraulic circuit further includes a second clamp disposed on the input pipe and used to control the on / off state of the input pipe; or / and

[0021] The flow electro-hydraulic circuit also includes a third air filter disposed at the end of the input pipeline away from the delivery pipeline.

[0022] Optionally, the flow electro-hydraulic circuit further includes a fourth air filter disposed at the end of the air inlet pipe away from the delivery pipe; or / and

[0023] The flow electro-hydraulic circuit also includes a third pipe clamp disposed on the delivery pipeline and used to control the on / off state of the delivery pipeline.

[0024] A flow electroporation device includes a controller, a sample container, a first collection container, the flow electroporation fluid path, a drive pump for mounting the collection line or the delivery line to drive fluid flow, and an electrode holder for mounting the flow electroporation container and electroporating the sample liquid inside the flow electroporation container; the flow electroporation fluid path, the drive pump, and the electrode holder are all connected to the controller.

[0025] The flow electroporation circuit provided by this utility model includes a flow electroporation container placed in an electrode holder; a delivery pipeline, one end of which is connected to the first end of the flow electroporation container and the other end of which is connected to a sample container for holding sample liquid; a collection pipeline, one end of which is connected to the second end of the flow electroporation container and the other end of which is connected to a first collection container; the collection pipeline or delivery pipeline is placed on a drive pump; and an air inlet pipeline, one end of which is connected to the delivery pipeline and the other end of which is connected to the atmosphere.

[0026] In this invention, full-flow electro-polarization can be achieved through the sequentially connected delivery pipe, flow electro-polarization container, and collection pipe in the flow electro-polarization circuit. Simultaneously, semi-flow electro-polarization can be achieved through the cooperation of the air inlet pipe connected to the delivery pipe. Thus, full-flow and semi-flow electro-polarization can be achieved through a single electro-polarization circuit, improving the compatibility of the flow electro-polarization device. Furthermore, since the flow electro-polarization circuit is a single integrated circuit, during installation, it is only necessary to place the flow electro-polarization container in the electrode clamp and the collection pipe or delivery pipe on the drive pump to initiate electro-polarization operation. Moreover, when the flow electro-polarization circuit needs to be replaced, only the entire circuit needs to be replaced, improving the convenience of installation or replacement. Therefore, this invention improves the ease of installation or replacement of the flow electro-polarization circuit while also enhancing the compatibility of the flow electro-polarization device, thereby increasing electro-polarization efficiency. Attached Figure Description

[0027] The present invention will be further described below with reference to the accompanying drawings and embodiments.

[0028] Figure 1 This is a schematic diagram of the structure of a flow electrostatic device provided in an embodiment of the present invention.

[0029] Figure 2 This is a schematic diagram of the structure of a flow electro-conversion device provided in another embodiment of the present invention.

[0030] Figure 3 This is a schematic diagram of the structure of a flow electrostatic device provided in another embodiment of the present invention.

[0031] The reference numerals in the accompanying drawings are as follows:

[0032] 100. Flow electro-hydraulic circuit; 110. Flow electro-hydraulic container; 120. Delivery line; 121. Third clamp; 130. Collection line; 131. First section; 132. Pump section; 133. Second section; 140. Air inlet line; 141. Fourth air filter; 150. First sampling line; 151. Sealing element; 152. First air filter; 160. Second sampling line; 161. Sampling drip chamber; 162. First clamp; 163. Second air filter; 170, inlet pipe; 171, second pipe clamp; 172, third air filter; 200, electrode clamp; 310, sample container; 320, first collection container; 330, second collection container; 400, drive pump; 510, first switching valve; 520, second switching valve; 530, third switching valve; 540, fourth switching valve; 600, pressure sensor; 710, first bubble sensor; 720, second bubble sensor. Detailed Implementation

[0033] To make the technical problems solved, technical solutions, and beneficial effects of this utility model clearer, the present utility model will be further described in detail below with reference to the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are only used to explain this utility model and are not intended to limit this utility model.

[0034] like Figure 1 As shown, one embodiment of this utility model provides a flow electro-hydraulic circuit 100, comprising:

[0035] The flow electrophoresis container 110 is placed in the electrode clamp 200;

[0036] The delivery pipeline 120 is connected at one end to the first end of the flow electroporation container 110 and at the other end to the sample container 310 for holding the sample liquid.

[0037] A collection pipe 130 is connected at one end to the second end of the flow electrophoresis container 110 and at the other end to the first collection container 320; the collection pipe 130 or the delivery pipe 120 is mounted on the drive pump 400, and

[0038] The intake pipe 140 is connected at one end to the delivery pipe 120 and at the other end to the atmosphere.

[0039] Understandably, the electrode clamp 200 may include a conductive module and a temperature control module. In some embodiments, the electrode clamp 200 has a clamping space that matches the flow electroporation container 110 and is used to clamp the flow electroporation container 110. The conductive module is used to conduct electricity to the positive and negative plates of the flow electroporation container 110 after the flow electroporation container 110 is clamped in the clamping space, so as to input electrical pulses to the flow electroporation container 110, thereby realizing electroporation of cells within the flow electroporation container 110. The temperature control module may be a semiconductor cooling chip or other components. By cooling the cold end of the temperature control module, the temperature of the sample solution within the flow electroporation container 110 can be controlled to remain within a preset temperature range, avoiding damage to cells in the sample solution due to overheating, thereby increasing the duration of electroporation and achieving continuous high-throughput electroporation. At the same time, the electrode clamp 200 may also include a heat sink to dissipate heat from the hot end of the temperature control module.

[0040] The collection pipeline 130 can be placed on the drive pump 400, and the delivery pipeline 120 can also be placed on the drive pump 400, as long as the drive pump 400 can drive the fluid (including liquid and gas, etc.) in the delivery pipeline 120, the flow electrostatic container 110 and the collection pipeline 130 connected in sequence to flow.

[0041] The sample container 310 and the delivery pipeline 120 can be connected via a Luer connector and / or a flexible Luer connector. The first collection container 320 and the collection pipeline 130 can also be connected via a Luer connector and / or a flexible Luer connector. The air inlet pipeline 140 and the delivery pipeline 120 can be connected via a tee connector.

[0042] In this embodiment, when performing full-flow electrospinning using the flow electrospinning liquid path 100, the delivery pipeline 120, the flow electrospinning container 110, and the collection pipeline 130, which are connected in sequence, can be controlled to be connected, and the electrode clamp 200 can be started. At the same time, while the drive pump 400 drives the sample liquid in the sample container 310 to continuously flow through the flow electrospinning container 110 through the delivery pipeline 120, the electrode clamp 200 also electrospins the sample liquid in the flow electrospinning container 110, and the sample liquid in the flow electrospinning container 110 after electrospinning is continuously collected in the first collection container 320 through the collection pipeline 130, thereby completing the full-flow electrospinning.

[0043] Meanwhile, when using the flow electroporation liquid path 100 for semi-flow electroporation, firstly, a preset volume of sample liquid in the sample container 310 is controlled to enter the flow electroporation container 110 through the delivery pipe 120. Then, the sample liquid in the flow electroporation container 110 is electroporated through the electrode clamp 200. After that, the collection pipe 130 is controlled to be connected to the atmosphere through the air inlet pipe 140, and the drive pump 400 is controlled to drive the sample liquid in the flow electroporation container 110 after electroporation to be collected into the first collection container 320 through the collection pipe 130, thereby completing the semi-flow electroporation.

[0044] In the above embodiments of this utility model, full-flow electro-polarization can be achieved through the sequentially connected delivery pipe 120, flow electro-polarization container 110, and collection pipe 130 in the flow electro-polarization circuit 100. Simultaneously, semi-flow electro-polarization can be achieved through the cooperation of the air inlet pipe 140 connected to the delivery pipe 120. Thus, full-flow and semi-flow electro-polarization can be achieved through the same electro-polarization circuit 100, improving the compatibility of the flow electro-polarization device. Furthermore, since the flow electro-polarization circuit 100 is a single integrated circuit, during installation, only the flow electro-polarization container 110 needs to be placed in the electrode clamp 200, and the collection pipe 130 or delivery pipe 120 needs to be placed on the drive pump 400 to achieve electro-polarization operation. Moreover, when the flow electro-polarization circuit 100 needs to be replaced, only the entire circuit needs to be replaced, improving the convenience of installation and replacement. Therefore, this invention improves the ease of installation or replacement of the flow electro-hydraulic circuit 100, while also enhancing the compatibility of the flow electro-hydraulic device, thereby increasing the electro-hydraulic efficiency.

[0045] like Figure 1 As shown, in one embodiment, the delivery pipeline 120 is placed on the first switching valve 510; the collection pipeline 130 is placed on the second switching valve 520; the air inlet pipeline 140 is placed on the third switching valve 530, one end of the air inlet pipeline 140 is connected to the first connection point of the delivery pipeline 120, and the other end is connected to the atmosphere; the first connection point is located between the first switching valve 510 and the flow electrostatic precipitator 110, and the drive pump 400 is located between the first connection point and the second switching valve 520.

[0046] In this embodiment, when using the flow electroporation circuit 100 for full-flow electroporation, the first switching valve 510 and the second switching valve 520 can be opened, and the third switching valve 530 can be closed to connect the delivery line 120 and the collection line 130. The electrode clamp 200 is also activated so that while the drive pump 400 drives the sample liquid in the sample container 310 to continuously flow through the delivery line 120 to the flow electroporation container 110, the electrode clamp 200 also electroporates the sample liquid in the flow electroporation container 110. The sample liquid that has completed electroporation in the flow electroporation container 110 is continuously collected into the first collection container 320 through the collection line 130, thereby realizing full-flow electroporation.

[0047] When using the flow electroporation circuit 100 for semi-flow electroporation, firstly, the first switching valve 510 and the second switching valve 520 are opened, and the third switching valve 530 is closed, so that the delivery line 120 and the collection line 130 are connected. The sample liquid in the sample container 310 is driven by the drive pump 400 to enter the flow electroporation container 110 through the delivery line 120. After confirming that the sample liquid output from the sample container 310 has reached the preset volume, the first switching valve 510 is closed, and the second switching valve 520 and the third switching valve 530 are opened, so that the delivery line 120 is connected to the atmosphere through the air inlet line 140. Finally, the drive pump 400 drives all the remaining sample liquid in the delivery line 120 to be emptied. After the flow electroporation container 110 is installed, the first switching valve 510, the second switching valve 520, and the third switching valve 530 are closed, and the electrode clamp 200 is activated to conduct electricity to the positive and negative plates of the flow electroporation container 110 and input electrical pulses, thereby electroporating the cells in the sample solution in the flow electroporation container 110. After confirming that the electroporation is completed, the first switching valve 510 is closed, and the second switching valve 520 and the third switching valve 530 are opened, so that the collection pipe 130 is connected to the atmosphere through the air inlet pipe 140, and the drive pump 400 is controlled to drive the sample solution in the flow electroporation container 110 that has completed electroporation to be collected into the first collection container 320 through the collection pipe 130. Semi-flow electroporation can also be achieved.

[0048] In the above embodiments, the sample container 310 can be mounted on the weighing module, and the sample liquid output by the sample container 310 can be weighed to determine whether the preset volume has been reached. Furthermore, the flow rate, velocity, and driving time of the driving pump can also be calculated to determine whether the preset volume of sample liquid output by the sample container 310 has been reached.

[0049] like Figure 1 As shown, in one embodiment, the collection line 130 is placed on the drive pump 400.

[0050] In one embodiment, the flow electro-hydraulic circuit 100 further includes a fourth air filter 141 disposed at the end of the air inlet pipe 140 away from the delivery pipe 120. The fourth air filter 141 is in communication with the atmosphere and is used to filter the air entering the flow electro-hydraulic circuit 100 through the air inlet pipe 140 to prevent foreign objects from entering.

[0051] like Figure 1 As shown, in one embodiment, the flow electro-hydraulic circuit 100 further includes a third clamp 121 disposed on the delivery line 120 and used to control the on / off state of the delivery line 120. The third clamp 121 may be located between the sample container 310 and the first connection point, thereby controlling the on / off state of the delivery line 120 when it is not yet placed on the first switching valve 510, preventing the sample liquid in the sample container 310 from flowing out. The third clamp 121 may be a Robert clamp.

[0052] like Figure 2 and Figure 3 As shown, in one embodiment, the flow electroporation circuit 100 further includes a first sampling line 150 placed on a fourth switching valve 540 and used for sampling the sample solution before and / or after electroporation; the first sampling line 150 connects to a second connection point of the delivery line 120, the second connection point being located between the flow electroporation container 110 and the first connection point; the drive pump 400 is located between the second connection point and the second switching valve 520. It is understood that the first sampling line 150 and the delivery line 120 can be connected via a tee connector. A switching valve can be provided between the second connection point and the first connection point to control the flow path of the delivery line 120 at that location.

[0053] When performing full-flow electroporation using the flow electroporation circuit 100, the sample solution continuously flows through the delivery line 120 through the flow electroporation container 110 and the collection line 130, and is collected in the first collection container 320. When the fourth switch valve 540 is opened, the sample solution before electroporation can be sampled through the first sampling line 150. When performing semi-flow electroporation using the flow electroporation circuit 100, the sample solution enters the flow electroporation container 110 through the delivery line 120. After electroporation is completed in the flow electroporation container 110, it enters other lines. Before completing electroporation, the sample solution remains in the flow electroporation container 110. At this time, when the sample solution enters the flow electroporation container 110 through the delivery line 120, the fourth switch valve 540 can be opened, and the first sampling line 150 can then sample the sample solution before electroporation. After the sample solution has been electrospun in the flow electrospinning container 110, the fourth switch valve 540 is opened, and the first sampling line 150 can also sample the electrospun sample solution.

[0054] like Figure 2 As shown, in one embodiment, the flow electro-hydraulic circuit 100 further includes a sealing member 151 disposed at the end of the first sampling line 150 away from the delivery line 120 and used to block the first sampling line 150. It can be understood that the sealing member 151 is used to block the end of the first sampling line 150 away from the delivery line 120 when sampling is not being performed through the first sampling line 150, thus preventing foreign matter from entering the flow electro-hydraulic circuit 100. In one embodiment, the sealing member 151 can be a Luer plug.

[0055] like Figure 2 As shown, in one embodiment, the flow electroporation circuit 100 further includes a first air filter 152 disposed at the end of the first sampling line 150 away from the delivery line 120. Understandably, the first air filter 152 is in communication with the atmosphere and is used to filter the air entering the flow electroporation circuit 100 through the first sampling line 150, preventing contamination of the sample liquid in the first sampling line 150 and the delivery line 120. With the first air filter 152 disposed on the first sampling line 150, the first sampling line 150 can replace the air inlet line 140 for air intake during the semi-flow electroporation process, thereby achieving another operating mode of the semi-flow electroporation.

[0056] like Figure 3As shown, in one embodiment, the flow electroporation circuit 100 further includes a second collection container 330 disposed at the end of the first sampling line 150 away from the delivery line 120. Understandably, the second collection container 330 can also be connected to the first sampling line 150 via a Luer connector and / or a flexible Luer connector. The second collection container 330 can be used to hold the sample solution before electroporation or after electroporation. That is, in semi-flow electroporation mode, the sample solution before electroporation can be sampled through the first sampling line 150 during the process of the sample solution entering the flow electroporation container 110, and the sampled sample solution before electroporation can be delivered to the second collection container 330. After the sample solution in the flow electroporation container 110 is electroporated by the electrode clamp 200, the drive pump 400 can be controlled to rotate in reverse, thereby driving the electroporated sample solution in the flow electroporation container 110 to flow into the second collection container 330 through the first sampling line 150.

[0057] like Figure 2 and Figure 3 As shown, in one embodiment, the flow electroporation circuit 100 further includes a second sampling line 160; the second sampling line 160 connects to a third connection point of the collection line 130, the third connection point being located between the first collection container 320 and the second switching valve 520. It is understood that the second sampling line 160 and the collection line 130 can be connected via a tee connector. In both semi-flow and full-flow electroporation modes, after the sample liquid in the flow electroporation container 110 is electroporated using the electrode clamp 200, the sample liquid after electroporation is sampled through the second sampling line 160 during the flow of the electroporated sample liquid from the flow electroporation container 110 to the first collection container 320.

[0058] like Figure 2 and Figure 3 As shown, in one embodiment, the flow electroporation circuit 100 further includes a sampling dropper 161 disposed on the second sampling line 160 and used to hold the electroporated sample solution. The sampling dropper 161 is used to hold the sampled electroporated sample solution.

[0059] like Figure 2 and Figure 3 As shown, in one embodiment, the flow electro-hydraulic circuit 100 further includes a first clamp 162 disposed on the second sampling line 160 and used to control the on / off state of the second sampling line 160. The first clamp 162 may be a Robert clamp.

[0060] like Figure 2 and Figure 3As shown, in one embodiment, the flow electro-hydraulic circuit 100 further includes a second air filter 163 disposed at the end of the second sampling line 160 away from the collection line 130. The second air filter 163 is in communication with the atmosphere to filter the air entering from the second sampling line 160, so as to avoid contamination of the sample liquid in the second sampling line 160 and the collection line 130.

[0061] like Figures 1 to 3 As shown, in one embodiment, the collection pipeline 130 includes a first pipe section 131, a pump pipe section 132, and a second pipe section 133 connected in sequence. The first pipe section 131 is placed on the second switching valve 520, and the pump pipe section 132 is placed on the drive pump 400. The end of the first pipe section 131 away from the pump pipe section 132 is connected to the first collection container 320, and the end of the second pipe section 133 away from the pump pipe section 132 is connected to the flow electrostatic discharge container 110. It is understood that the material of the pump pipe section 132 can be a soft material that matches the precise control of the drive pump 400. By installing the pump pipe section 132 to the drive pump, the drive pump 400 can more accurately control the fluid it drives. The first pipe section 131 and the pump pipe section 132, and the second pipe section 133 and the pump pipe section 132, can be connected by connectors, such as pump pipe connectors. The first pipe section 131 and the pump pipe section 132, as well as the second pipe section 133 and the pump pipe section 132, can also be integrally formed.

[0062] like Figure 2 and Figure 3 As shown, in one embodiment, the flow electroporation circuit 100 further includes at least one input line 170 for inputting a liquid to be added; the input line 170 is connected to a fourth connection point of the delivery line 120, the fourth connection point being located between the first switching valve 510 and the flow electroporation container 110. It is understood that each of the input lines 170 can be aseptically connected to a liquid to be added, thereby allowing different substances to be added during the electroporation process as needed.

[0063] like Figure 2 and Figure 3 As shown, in one embodiment, the flow electro-hydraulic circuit 100 further includes a second clamp 171 disposed on the input pipe 170 and used to control the on / off state of the input pipe 170.

[0064] like Figure 2 and Figure 3As shown, in one embodiment, the flow electro-hydraulic circuit 100 further includes a third air filter 172 disposed at the end of the input pipe 170 away from the delivery pipe 120. The third air filter 172 is in communication with the atmosphere to filter the air entering from the input pipe 170 and avoid contamination of the sample liquid.

[0065] like Figure 1 As shown, one embodiment of this utility model also provides a flow electroporation device, including a controller, a sample container 310, a first collection container 320, the flow electroporation fluid path 100, a drive pump 400 for mounting the collection pipeline 130 or the delivery pipeline 120 to drive fluid flow, and an electrode clamp 200 for mounting the flow electroporation container 110 and electroporating the sample liquid in the flow electroporation container 110; the flow electroporation fluid path 100, the drive pump 400 and the electrode clamp 200 are all connected to the controller.

[0066] Understandably, the drive pump 400 includes, but is not limited to, a peristaltic pump, which can drive fluid flow without contacting the liquid, thus preventing contamination of the liquid. Understandably, the drive pump 400 can also be other types of liquid pumps, as long as they achieve the effect of driving fluid flow. Understandably, the electrode clamp 200 may include a conductive module and a temperature control module. In some embodiments, the electrode clamp 200 has a clamping space that matches the flow electroporation container 110 and is used to clamp the flow electroporation container 110. The conductive module is used to conduct electricity to the positive and negative plates of the flow electroporation container 110 after the flow electroporation container 110 is clamped in the clamping space, so as to input electrical pulses to the flow electroporation container 110, thereby realizing electroporation of the cells within the flow electroporation container 110. The temperature control module can be a semiconductor cooling chip or similar component. By cooling the cold end of the temperature control module, the temperature of the sample solution in the flow electroporation container 110 can be controlled to remain within a preset temperature range, avoiding damage to the cells in the sample solution due to overheating, thereby increasing the duration of electroporation and achieving continuous high-throughput electroporation. Simultaneously, the electrode clamp 200 may also include a heat sink to dissipate heat from the hot end of the temperature control module.

[0067] Each module in the aforementioned controller can be implemented entirely or partially through software, hardware, or a combination thereof. These modules can be embedded in the controller in hardware form or independent of it, or stored in the controller in software form, so that the controller can call and execute the corresponding operations of each module.

[0068] The flow electrospinning device provided by this utility model can achieve full-flow electrospinning through the sequentially connected conveying pipe 120, flow electrospinning container 110, and collecting pipe 130 in the electrospinning pipeline. Simultaneously, it can achieve semi-flow electrospinning through the cooperation of the air inlet pipe 140 connected to the conveying pipe 120. Thus, full-flow and semi-flow electrospinning can be achieved through the same electrospinning pipeline 100, improving the compatibility of the flow electrospinning device. Furthermore, since the flow electrospinning liquid circuit 100 is a single integrated pipeline, during installation, it is only necessary to place the flow electrospinning container 110 in the electrode clamp 200 and place the collecting pipe 130 or the conveying pipe 120 on the drive pump 400 to achieve electrospinning operation. Moreover, when the flow electrospinning liquid circuit 100 needs to be replaced, only the entire assembly needs to be replaced, improving the convenience of installation and replacement. Therefore, this invention improves the ease of installation or replacement of the flow electro-hydraulic circuit 100, while also enhancing the compatibility of the flow electro-hydraulic device, thereby increasing the electro-hydraulic efficiency.

[0069] like Figure 1 As shown, in one embodiment, the flow electrostatic precipitator further includes a first switching valve 510 for controlling the on / off state of the delivery pipeline 120; a second switching valve 520 for controlling the on / off state of the collection pipeline 130; and a third switching valve 530 for controlling the on / off state of the air intake pipeline 140. The first switching valve 510, the second switching valve 520, and the third switching valve 530 are all connected to the controller. One end of the air intake pipeline 140 is connected to the first connection point of the delivery pipeline 120, and the other end is connected to the atmosphere. The drive pump 400 is located between the first connection point and the second switching valve 520.

[0070] like Figure 2 and Figure 3As shown, in one embodiment, the flow cytometry apparatus further includes a pressure sensor 600 and a first bubble sensor 710 disposed on the delivery pipeline 120, and a second bubble sensor 720 disposed on the collection pipeline 130. The pressure sensor 600 is used to detect the pressure value of the liquid flowing through the delivery pipeline 120, thereby adjusting the driving power of the drive pump 400 based on the measured pressure value. The first bubble sensor 710 is used to detect the flow state of the liquid flowing through the delivery pipeline 120, that is, the first bubble sensor 710 can be used to detect whether the sample liquid in the sample container 310 flows through the delivery pipeline 120, whether it has flowed out completely, and the flow direction, etc. The second bubble sensor 720 is used to detect the flow state of the liquid flowing through the collection pipeline 130, that is, the second bubble sensor 720 can be used to detect whether the sample liquid in the flow cytometry container 110 flows through the collection pipeline 130, whether it has flowed out completely, and the flow direction, etc. Therefore, by using the first bubble sensor 710 and the second bubble sensor 720, it can be determined whether the relevant operation steps of driving the fluid flow by the drive pump 400 have been completed, and further control the operation of the drive pump 400 or the switching of each valve to carry out subsequent steps.

[0071] An embodiment of this utility model also provides a flow electrostatics method applied to the controller of the flow electrostatics device, the flow electrostatics method comprising the following steps S100-S200:

[0072] S100: Receive the first intermittent semi-flow electrospinning signal, control the sample liquid of a preset volume in the sample container 310 to enter the flow electrospinning container 110 via the delivery pipe 120, and electrospin the sample liquid in the flow electrospinning container 110 through the electrode clamp 200. After electrospinning, control the delivery pipe 120 to connect to the atmosphere through the air inlet pipe 140, and control the drive pump 400 to drive the electrospinned sample liquid in the flow electrospinning container 110 to be collected into the first collection container 320 through the collection pipe 130. The preset volume can be set according to actual conditions. For example, the preset volume can be determined based on the volume of the flow electrospinning container 110 and the delivery pipe 120. For example, a preset volume can be set equal to the volume of the flow electroporation container 110. At this time, the preset volume of sample liquid in the sample container 310 enters the flow electroporation container 110 through the delivery pipe 120, which means that the preset volume of sample liquid has filled the flow electroporation container. This indicates that the process of injecting the sample into the flow electroporation container 110 has been completed, and there is no need to add sample liquid to the flow electroporation container 110. Then, the sample liquid in the flow electroporation container can be electroporated by the electrode clamp.

[0073] S200: Receive a continuous full-flow electrospinning signal, control the delivery line 120 and collection line 130 to remain open, and control the electrode clamp 200 to start. While controlling the drive pump 400 to drive the sample liquid in the sample container 310 to continuously flow through the delivery line 120 through the flow electrospinning container 110, the electrode clamp 200 also electrospins the sample liquid in the flow electrospinning container 110, and the sample liquid in the flow electrospinning container 110 after electrospinning is continuously collected into the first collection container 320 through the collection line 130.

[0074] The flow electrospinning method provided by this utility model can achieve full-flow electrospinning through the sequentially connected delivery pipe 120, flow electrospinning container 110, and collection pipe 130 in the electrospinning pipeline. Simultaneously, semi-flow electrospinning can be achieved through the cooperation of the air inlet pipe 140 connected to the delivery pipe 120. Thus, full-flow and semi-flow electrospinning can be compatible through the same electrospinning pipeline 100, improving the compatibility of the flow electrospinning device. Furthermore, since the flow electrospinning liquid circuit 100 is a single integrated pipeline, during installation, only the flow electrospinning container 110 needs to be placed in the electrode clamp 200, and the collection pipe 130 or delivery pipe 120 needs to be placed on the drive pump 400 to achieve electrospinning operation. Moreover, when the flow electrospinning liquid circuit 100 needs to be replaced, only the entire system needs to be replaced, improving the convenience of installation and replacement. Therefore, this invention improves the ease of installation or replacement of the flow electro-hydraulic circuit 100, while also enhancing the compatibility of the flow electro-hydraulic device, thereby increasing the electro-hydraulic efficiency.

[0075] In one embodiment, in step S100, a preset volume of sample liquid in the control sample container 310 enters the flow electroporation container 110 via the delivery pipe 120. The sample liquid in the flow electroporation container 110 is electroporated by the electrode clamp 200. After electroporation is completed, the control delivery pipe 120 is connected to the atmosphere through the air inlet pipe 140, and the drive pump 400 drives the electroporated sample liquid in the flow electroporation container 110 to be collected into the first collection container 320 through the collection pipe 130. This includes the following steps S110-S140:

[0076] S110, the control delivery line 120 is turned on, and the drive pump 400 drives the sample liquid in the sample container 310 to enter the flow electrophoresis container 110 through the delivery line 120. That is, after receiving the first intermittent semi-flow electrophoresis signal, the sample liquid can be delivered into the flow electrophoresis container 110.

[0077] S120. After confirming that the sample liquid output from the sample container 310 has reached the preset volume, control the sample container 310 to stop outputting the sample liquid, and control the delivery pipeline 120 to connect to the atmosphere through the air inlet pipeline 140, and drive the pump 400 to drive the remaining sample liquid in the delivery pipeline 120 to be emptied into the flow electrotransfer container 110. Understandably, after the sample solution enters the flow electroporation container 110 through the delivery line 120, at least a portion will remain in the delivery line 120 when the sample container 310 stops outputting the sample solution. In order to ensure that all the sample solution of the preset volume output by the sample container 310 enters the flow electroporation container 110, the delivery line 120 can be connected to the atmosphere through the air inlet line 140. Then, when the drive pump 400 is driven to rotate, air enters the delivery line 120 through the air inlet line 140, and the sample solution remaining in the delivery line 120 is emptied into the flow electroporation container 110, thereby ensuring the accurate volume of the electroporated sample solution.

[0078] S130: After confirming that all sample solution in the delivery line 120 has been emptied, the air inlet line 140, delivery line 120, and collection line 130 are all closed, and the electrode clamp 200 is activated to electrolyze the cells in the sample solution placed in the flow cytometry container 110 within the electrode clamp 200. That is, after all the remaining sample solution in the delivery line 120 has been emptied into the flow cytometry container 110, the electrode clamp 200 can be activated to electrolyze the cells in the sample solution within the flow cytometry container 110.

[0079] S140. After confirming the completion of electroporation, the control delivery line 120 is connected to the atmosphere through the air inlet line 140, and the drive pump 400 is controlled to drive the sample liquid after electroporation in the flow electroporation container 110 to be collected into the first collection container 320 through the collection line 130. At this time, the sample container 310 is closed. During the process of the sample liquid after electroporation in the flow electroporation container 110 being collected into the first collection container 320 through the collection line 130, the collection line 130 maintains the pressure balance in the flow electroporation liquid path 100 through the air entering from the air inlet line 140, thereby ensuring that all the sample liquid in the flow electroporation container 110 is successfully collected into the first collection container 320 through the collection line 130. During this process, keeping the sample container 310 closed ensures that the sample liquid in the sample container 310 does not flow out into the flow electroporation container 110, thereby avoiding mixing of the sample liquid before and after electroporation.

[0080] In one embodiment, the flow electrostatic discharge device further includes a first switching valve 510 for controlling the on / off state of the delivery pipeline 120; a second switching valve 520 for controlling the on / off state of the collection pipeline 130; and a third switching valve 530 for controlling the on / off state of the air intake pipeline 140. The first switching valve 510, the second switching valve 520, and the third switching valve 530 are all connected to the controller. One end of the air intake pipeline 140 is connected to the first connection point of the delivery pipeline 120, and the other end is connected to the atmosphere. The drive pump 400 is located between the first connection point and the second switching valve 520.

[0081] In step S100, the control delivery line 120 and the collection line 130 are continuously connected, and the control electrode clamp 200 is activated. This allows the sample liquid in the sample container 310 to continuously flow through the flow electroporation container 110 via the delivery line 120, while the sample liquid in the flow electroporation container 110 is electroporated via the electrode clamp 200. The electroporated sample liquid in the flow electroporation container 110 is then continuously collected into the first collection container 320 via the collection line 130. This includes the following steps S111-S141:

[0082] S111: The first switching valve 510 and the second switching valve 520 are opened, and the third switching valve 530 is closed, so that the delivery line 120 is connected to the flow electroporation container 110. The sample liquid in the sample container 310 is driven by the drive pump 400 to enter the flow electroporation container 110 through the delivery line 120. Understandably, after receiving the first intermittent semi-flow electroporation signal, the first switching valve 510 opens to connect the delivery line 120, ensuring that the sample liquid in the sample container 310 can enter the flow electroporation liquid path 100. The second switching valve 520 opens to connect the collection line 130, thereby ensuring that the collection line 130 is connected to the delivery line through the flow electroporation container 110. This allows the drive pump 400 on the collection line 130 to drive the sample liquid from the sample container 310 into the flow electroporation container 110 when it starts. Understandably, closing the third switch valve 530 closes the air inlet pipe 140, preventing air and sample liquid from entering the flow electro-liquid circuit 100 simultaneously, thereby interfering with the sample liquid injection process.

[0083] S121. After confirming that the sample liquid output from the sample container 310 has reached a preset volume, the first switching valve 510 is closed to stop the sample container 310 from outputting sample liquid. The second switching valve 520 and the third switching valve 530 are opened to allow the delivery pipeline 120 to be connected to the atmosphere through the air inlet pipeline 140, and the collection pipeline 130 to be connected to the delivery pipeline 120 through the flow electrochemical converter 110. This allows the drive pump 400 on the collection pipeline 130 to start, driving outside air into the delivery pipeline 120 through the air inlet pipeline 140, thereby pushing the residual sample liquid in the delivery pipeline 120 to be discharged into the flow electrochemical converter 110. It can be understood that in the above embodiment, the sample container 310 can be mounted on a weighing module, and the sample container 310 can be weighed to determine whether the sample liquid output from the sample container 310 has reached the preset volume. In addition, the flow rate, velocity, and driving time of the driving pump can be calculated to determine whether the sample liquid output from the sample container 310 has reached the preset volume.

[0084] S131. After the control drive pump 400 drives all the sample liquid remaining in the delivery pipeline 120 to be emptied into the flow electroporation container 110, the first switch valve 510, the second switch valve 520 and the third switch valve 530 are controlled to close, so that the air inlet pipeline 140, the delivery pipeline 120 and the collection pipeline 130 are all closed, and the electrode clamp 200 is controlled to start, so as to conduct electricity to the positive and negative plates of the flow electroporation container 110 and input electrical pulses, thereby electroporating the cells in the sample liquid in the flow electroporation container 110.

[0085] S141. After confirming the completion of electroporation, the first switch valve 510 is closed to shut off the sample container 310, the third switch valve 530 is opened to connect the delivery line 120 to the atmosphere via the air inlet line 140, and the second switch valve 520 is opened to connect the collection line 130 to the first collection container 320. The drive pump 400 is then controlled to drive the sample solution in the flow electroporation container 110, after electroporation, to be collected in the first collection container 320 via the collection line 130. Understandably, the closure of the first switch valve 510 shuts off the delivery line 120, preventing the sample solution in the sample container 310 from entering the flow electroporation liquid path 100. The opening of the second switch valve 520 opens the collection line 130, ensuring that the drive pump 400 can drive the sample solution in the flow electroporation container 110 to flow into the first collection container 320 via the collection line 130. The third switching valve 530 is opened to open the air inlet pipe 140, so as to ensure the pressure balance in the flow electroporation liquid path 100 when the sample liquid in the flow electroporation container 110 flows into the first collection container 320, thereby allowing the sample liquid to flow into the first collection container 320 through the collection pipe 130 smoothly.

[0086] In one embodiment, the flow electrostatic discharge device further includes a first switching valve 510 for controlling the on / off state of the delivery pipeline 120; a second switching valve 520 for controlling the on / off state of the collection pipeline 130; and a third switching valve 530 for controlling the on / off state of the air intake pipeline 140. The first switching valve 510, the second switching valve 520, and the third switching valve 530 are all connected to the controller. One end of the air intake pipeline 140 is connected to the first connection point of the delivery pipeline 120, and the other end is connected to the atmosphere. The drive pump 400 is located between the first connection point and the second switching valve 520.

[0087] Further, in step S200, the control delivery line 120 and collection line 130 are continuously connected, and the control electrode clamp 200 is activated. This allows the sample liquid in the sample container 310 to continuously flow through the flow electroporation container 110 via the delivery line 120 while the control drive pump 400 drives the sample liquid in the flow electroporation container 110. Simultaneously, the electrode clamp 200 electroporates the sample liquid in the flow electroporation container 110, and the electroporated sample liquid in the flow electroporation container 110 is continuously collected into the first collection container 320 via the collection line 130. This includes:

[0088] S210: Receive a continuous full-flow electrospinning signal, control the first switching valve 510 and the second switching valve 520 to open, the third switching valve 530 to close, and control the electrode clamp 200 to start. While controlling the drive pump 400 to drive the sample liquid in the sample container 310 to flow through the delivery pipeline 120 through the flow electrospinning container 110, the electrode clamp 200 continuously electrospins the sample liquid flowing through the flow electrospinning container 110, and the sample liquid that has completed electrospinning in the flow electrospinning container 110 is continuously collected into the first collection container 320 through the collection pipeline 130. Understandably, when the first switching valve 510 and the second switching valve 520 are opened, the delivery pipeline 120 is connected to the collection pipeline 130 via the flow electroporation container 110. At this time, the drive pump 400 starts, and the sample liquid in the sample container 310 continuously enters the flow electroporation container 110 through the delivery pipeline 120. After electroporation in the flow electroporation container 110, it continues to flow from the flow electroporation container 110 into the first collection container 320 through the collection pipeline 130. During the above-mentioned full-flow electroporation process, the sample container 310 is continuously input into the flow electroporation container 110 through the delivery pipeline 120 for electroporation, and the sample liquid that has completed electroporation in the flow electroporation container 110 also continuously enters the first collection container 320 through the collection pipeline until the full-flow electroporation is completed.

[0089] In some embodiments, the delivery line 120 is placed on the drive pump 400; the flow electro-hydraulic circuit 100 further includes a first sampling line 150 connected to the delivery line 120 and a second sampling line 160 connected to the collection line 130; the flow electro-hydraulic circuit 100 further includes a second collection container 330 disposed at one end of the first sampling line 150 away from the delivery line 120.

[0090] Specifically, the delivery pipeline 120 is placed on the first switching valve 510; the collection pipeline 130 is placed on the second switching valve 520; the air inlet pipeline 140 is placed on the third switching valve 530, one end of the air inlet pipeline 140 is connected to the first connection point of the delivery pipeline 120, and the other end is connected to the atmosphere; the first connection point is located between the first switching valve 510 and the flow electroporation container 110, and the drive pump 400 is located between the first connection point and the second switching valve 520; the flow electroporation liquid circuit 100 also includes a first sampling pipeline 150 placed on the fourth switching valve 540 and used to sample the sample liquid before and / or after electroporation. The first sampling line 150 is connected to the second connection point of the delivery line 120, and the second connection point is located between the flow electro-converter 110 and the first connection point; the drive pump 400 is located between the second connection point and the second switching valve 520; the flow electro-converter liquid circuit 100 also includes a second collection container 330 disposed at one end of the first sampling line 150 away from the delivery line 120; the flow electro-converter liquid circuit 100 also includes a second sampling line 160; the second sampling line 160 is connected to the third connection point of the collection line 130, and the third connection point is located between the first collection container 320 and the second switching valve 520.

[0091] The flow electrophoresis method further includes:

[0092] S300: Receive the second intermittent semi-flow electrospinning signal, control the sample liquid of a preset volume in the sample container 310 to enter the flow electrospinning container 110 through the delivery pipeline 120, and electrospin the sample liquid in the flow electrospinning container 110 through the electrode clamp 200. After the electrospinning is completed, control the collection pipeline 130 to connect to the atmosphere through the second sampling pipeline 160, and control the drive pump 400 to drive the sample liquid in the flow electrospinning container 110 to be collected into the second collection container 330 through the first sampling pipeline 150.

[0093] Understandably, in step S300, the sample liquid of a preset volume in the control sample container 310 enters the flow electroporation container 110 via the delivery pipeline 120, and the sample liquid in the flow electroporation container 110 is electroporated by the electrode clamp 200. The relevant content of this process can be referred to in steps S110-S130 and S111-S131 in the above embodiments.

[0094] In some embodiments, in step S300, the step of controlling the collection line 130 to connect to the atmosphere through the second sampling line 160 after electroporation is completed, and controlling the drive pump 400 to drive the sample liquid after electroporation in the flow electroporation container 110 to be collected into the second collection container 330 through the first sampling line 150, includes:

[0095] After confirming the completion of electroporation, the control collection line 130 is connected to the atmosphere through the second sampling line 160, and the drive pump 400 is controlled to drive the sample liquid after electroporation in the flow electroporation container 110 to be collected into the second collection container 330 through the first sampling line 150. At this time, the sample container 310 is closed. During the process of the sample liquid after electroporation in the flow electroporation container 110 being collected into the second collection container 330 through the first sampling line 150, the collection line 130 maintains the pressure balance in the flow electroporation liquid path 100 by the air entering from the second sampling line 160, thereby ensuring that all the sample liquid in the flow electroporation container 110 is successfully collected into the second collection container 330 through the second sampling line 160. During this process, keeping the sample container 310 closed ensures that the sample liquid in the sample container 310 does not flow out into the flow electroporation container 110, thereby avoiding mixing of the sample liquid before and after electroporation.

[0096] In some embodiments, in step S300, the step of controlling the collection line 130 to connect to the atmosphere through the second sampling line 160 after electroporation is completed, and controlling the drive pump 400 to drive the sample liquid after electroporation in the flow electroporation container 110 to be collected into the second collection container 330 through the first sampling line 150, includes:

[0097] After confirming the completion of electroporation, the first switching valve 510 and the third switching valve 530 are closed, while the second switching valve 520 and the fourth switching valve 540 are opened. This allows the collection line 130 to be connected to the atmosphere via the second sampling line 160. The drive pump 400 is then controlled to drive the electroporated sample solution in the flow electroporation container 110 to be collected in the second collection container 330 via the first sampling line 150. Understandably, closing the first switching valve 510 closes the sample container 310, preventing the sample solution in the sample container 310 from entering the flow electroporation liquid path 100. Opening the fourth switching valve 540 opens the first sampling line 150 to ensure that the drive pump 400 can drive the sample solution in the flow electroporation container 110 to flow into the second collection container 330 via the first sampling line 150. The second switching valve 520 is opened to conduct the second sampling line 160, so as to ensure the pressure balance in the flow electroporation liquid path 100 when the sample liquid in the flow electroporation container 110 flows into the second collection container 330, thereby allowing the sample liquid to flow into the second collection container 330 through the first sampling line 150 smoothly.

[0098] In one embodiment, the flow electro-hydraulic circuit 100 further includes at least one input line 170 for inputting the liquid to be added; the input line 170 is connected to a fourth connection point of the delivery line 120, the fourth connection point being located between the first switching valve 510 and the flow electro-hydraulic container 110.

[0099] Furthermore, the procedure following step S300 also includes:

[0100] When the input line 170 is turned on, the control drive pump 400 drives the culture medium solution connected in the input line 170 to enter the flow electroporation vessel 110 through the input line 170 to rinse the flow electroporation vessel 110.

[0101] After rinsing, the first sampling line 150 is turned on, and the drive pump 400 drives the rinsed culture medium solution in the flow electroporation container 110 through the first sampling line 150 into the second collection container 330. Understandably, after the rinsed culture medium solution in the flow electroporation container 110 enters the second collection container 330 through the first sampling line 150, step S300 can be repeated, allowing for multiple collections of the electroporated sample solution in the second collection container 330 until the collection target is achieved.

[0102] The control unit connects the collection line 130 and the first sampling line 150, and controls the drive pump 400 to drive the gas in the first collection container 320 through the collection line 130, the flow electroporation container 110, and the first sampling line 150. This pushes the remaining culture medium solution in the collection line 130, the flow electroporation container 110, and the first sampling line 150 into the second collection container 330, thereby emptying the flow electroporation circuit 100. Understandably, this step is performed after the collection target is achieved.

[0103] Furthermore, after step S200, the method further includes:

[0104] The control delivery line 120 and the collection line 130 are kept in continuous operation. The control drive pump 400 drives the culture medium solution introduced into the input line 170 to flow continuously through the delivery line 120 through the flow electroporation container 110 and be collected into the first collection container 320 through the collection line 130 to rinse the flow electroporation container 110.

[0105] After rinsing is completed, the collection line 130 and the first sampling line 150 are connected, and the drive pump 400 is controlled to drive the gas in the second collection container 330 to flow through the first sampling line 150, the flow electroporation container 110 and the collection line 130, so as to push the remaining culture medium solution in the first sampling line 150, the flow electroporation container 110 and the collection line 130 into the first collection container 320, thereby emptying the flow electroporation liquid path 100.

[0106] In one embodiment, the electrode clamp 200 includes a conductive module and a temperature control module.

[0107] Further, in steps S100 and S200, the electroporation of the sample solution in the flow electroporation container 110 using the electrode clamp 200 includes:

[0108] The conductive module conducts electrical pulses to the positive and negative plates of the flow electroporation container 110 to achieve electroporation of cells within the flow electroporation container 110. At the same time, the temperature control module controls the temperature of the sample solution within the flow electroporation container 110 to be maintained within a preset electroporation temperature range.

[0109] Understandably, the electrode clamp 200 may include a conductive module and a temperature control module. In some embodiments, the electrode clamp 200 has a clamping space that matches the flow electroporation container 110 and is used to clamp the flow electroporation container 110. The conductive module is used to conduct electricity to the positive and negative plates of the flow electroporation container 110 after the flow electroporation container 110 is clamped in the clamping space, so as to input electrical pulses to the flow electroporation container 110, thereby realizing electroporation of cells within the flow electroporation container 110. The temperature control module may be a semiconductor cooling chip or other components. By cooling the cold end of the temperature control module, the temperature of the sample solution within the flow electroporation container 110 can be controlled to remain within a preset temperature range, avoiding damage to cells in the sample solution due to overheating, thereby increasing the duration of electroporation and achieving continuous high-throughput electroporation. At the same time, the electrode clamp 200 may also include a heat sink to dissipate heat from the hot end of the temperature control module.

[0110] It should be understood that the sequence number of each step in the above embodiments does not imply the order of execution. The execution order of each process should be determined by its function and internal logic, and should not constitute any limitation on the implementation process of this utility model embodiment.

[0111] The above are merely embodiments of the flow electro-hydraulic circuit and device of this utility model, and are not intended to limit this utility model. Any modifications, equivalent substitutions, and improvements made within the spirit and principles of this utility model should be included within the protection scope of this utility model.

Claims

1. A flow electro-hydraulic circuit, characterized in that, include: A flow electrophoresis container is placed in an electrode fixture; The delivery pipeline is connected at one end to the first end of the flow electroporation container and at the other end to a sample container for holding the sample solution. A collection pipeline, one end connected to the second end of the flow electrophoresis container, and the other end connected to the first collection container; the collection pipeline or the delivery pipeline is mounted on a drive pump; and The air intake pipe is connected to the delivery pipe at one end and to the atmosphere at the other end.

2. The flow electro-hydraulic circuit according to claim 1, characterized in that, The delivery pipeline is placed on the first switch valve; the collection pipeline is placed on the second switch valve; the air inlet pipeline is placed on the third switch valve, one end of the air inlet pipeline is connected to the first connection point of the delivery pipeline, and the other end is connected to the atmosphere; the first connection point is located between the first switch valve and the flow electrostatic container, and the drive pump is located between the first connection point and the second switch valve.

3. The flow electro-hydraulic circuit according to claim 2, characterized in that, The flow electroporation circuit further includes a first sampling line placed on the fourth switching valve for sampling the sample solution before and / or after electroporation; the first sampling line is connected to a second connection point of the delivery line, the second connection point being located between the flow electroporation container and the first connection point; the drive pump is located between the second connection point and the second switching valve.

4. The flow electro-hydraulic circuit according to claim 3, characterized in that, The flow electro-hydraulic circuit further includes a sealing element disposed at the end of the first sampling line away from the delivery line and used to seal the first sampling line; or / and The flow electro-hydraulic circuit further includes a first air filter disposed at the end of the first sampling line away from the delivery line; or / and The flow electro-hydraulic circuit also includes a second collection container disposed at the end of the first sampling pipeline away from the delivery pipeline.

5. The flow electro-hydraulic circuit according to claim 2, characterized in that, The flow electro-hydraulic circuit also includes a second sampling line; the second sampling line is connected to a third connection point of the collection line, and the third connection point is located between the first collection container and the second switching valve.

6. The flow electro-hydraulic circuit according to claim 5, characterized in that, The flow electroporation circuit further includes a sampling dropper disposed on the second sampling tube for holding the sample solution after electroporation; or / and The flow electro-hydraulic circuit further includes a first clamp disposed on the second sampling line and used to control the on / off state of the second sampling line; or / and The flow electro-hydraulic circuit also includes a second air filter disposed at the end of the second sampling line away from the collection line, and the second air filter is in communication with the atmosphere.

7. The flow electro-hydraulic circuit according to claim 2, characterized in that, The collection pipeline includes a first pipe section, a pump pipe section, and a second pipe section connected in sequence; the first pipe section is positioned on the second switch valve, the pump pipe section is positioned on the drive pump, the end of the first pipe section away from the pump pipe section is connected to the first collection container, and the end of the second pipe section away from the pump pipe section is connected to the flow electrostatic container; or / and The flow electro-hydraulic circuit further includes at least one input line for inputting the liquid to be added; the input line is connected to a fourth connection point of the delivery line, the fourth connection point being located between the first switching valve and the flow electro-hydraulic container.

8. The flow electro-hydraulic circuit according to claim 7, characterized in that, The flow electro-hydraulic circuit further includes a second clamp disposed on the input pipe and used to control the on / off state of the input pipe; or / and The flow electro-hydraulic circuit also includes a third air filter disposed at the end of the input pipeline away from the delivery pipeline.

9. The flow electro-hydraulic circuit according to claim 1, characterized in that, The flow electro-hydraulic circuit further includes a fourth air filter disposed at the end of the air inlet pipe away from the delivery pipe; or / and The flow electro-hydraulic circuit also includes a third pipe clamp disposed on the delivery pipeline and used to control the on / off state of the delivery pipeline.

10. A flow electrophoresis device, characterized in that, The device includes a controller, a sample container, a first collection container, a flow electroporation circuit as described in any one of claims 1 to 9, a drive pump for mounting the collection circuit or the delivery circuit to drive fluid flow, and an electrode clamp for mounting the flow electroporation container and electroporating the sample liquid within the flow electroporation container; the flow electroporation circuit, the drive pump, and the electrode clamp are all connected to the controller.

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