An electric field generating device

Abstract

The utility model discloses an electric field generating device, including transparent partition, the transparent partition outer wall is provided with the first annular electrode piece, and the transparent partition inner wall is provided with the second annular electrode piece, and the transparent partition is located the vertical direction intermediate position of first annular electrode piece and second annular electrode piece, and the transparent partition top outer wall has opened a plurality of clamping grooves, and the clamping groove inner wall has inserted the culture dish, and the first annular electrode piece and second annular electrode piece outer wall are provided with electrically conductive wire joint respectively. The utility model lets electric field generating device can be in multiple culture dishes simultaneously with corresponding intensity electric field effect, improves cell electric field experiment efficiency, and through setting up the two radial symmetry annular electrode configuration of first annular electrode piece and second annular electrode piece, forms the concentric circle electric field of gradient decrease, ensures the consistency of symmetric position electric field condition, and compared with traditional flat plate electrode, this design can satisfy the control variable requirement of experimental group and control group more.

Description

Technical Field

[0001] This utility model relates to the field of biological experimental technology, and in particular to an electric field generating device. Background Technology

[0002] Tumor therapeutic electric fields (TTFields) are a novel approach to treating tumors using medium-frequency and low-intensity sinusoidal alternating electric fields. TTFields have been shown to inhibit tumor development and progression by suppressing cell mitosis, inhibiting DNA damage repair, and enhancing immune responses. They have been successfully applied in the clinical treatment of newly diagnosed glioblastoma and recurrent glioblastoma. However, the mechanisms of action of TTFields on cells require further investigation.

[0003] In basic research on TTFields, researchers often use in vitro cell experiments to investigate the effects of TTFields by culturing cells in culture dishes and applying an electric field to the dishes. A search revealed a patent application with patent number CN202310057355.3, which discloses a tumor therapeutic electric field (TTFields) cell experiment culture dish, including a culture dish lid, a culture dish body, an electric field electrode plate, and an electric field conductive wire. The culture dish lid and culture dish body are both circular. However, the above technical solution can only apply the corresponding electric field to a single culture dish at a time, and cannot simultaneously apply the corresponding electric field to multiple culture dishes containing the corresponding cells, resulting in low efficiency of cell experiments. Utility Model Content

[0004] The purpose of this invention is to address the shortcomings of existing technologies by proposing an electric field generating device.

[0005] To achieve the above objectives, the present invention adopts the following technical solution:

[0006] An electric field generating device includes a transparent partition. A first annular electrode sheet is disposed on the outer wall of the transparent partition, and a second annular electrode sheet is disposed on the inner wall of the transparent partition. The transparent partition is located at the midpoint between the first and second annular electrode sheets in the vertical direction. Multiple slots are formed on the top outer wall of the transparent partition, and a petri dish is inserted into the inner wall of the slot. Electrical wire connectors are respectively disposed on the outer walls of the first and second annular electrode sheets, and the electrical wire connectors are connected to a signal generator through wire lines.

[0007] As a further improvement of this utility model, the outer wall of the transparent partition is provided with multiple scales corresponding to the slots.

[0008] As a further improvement of this utility model: the outer wall of the first annular electrode sheet has a through hole, and a connecting wire is inserted into the inner wall of the through hole.

[0009] As a further embodiment of this utility model: one end of the connecting line is connected to a current probe, and the other end of the connecting line is connected to a host, and the current probe is electrically connected to the host through the connecting line.

[0010] As a further improvement of this utility model, multiple support plates are fixed to the bottom outer wall of the transparent partition.

[0011] As a further embodiment of this utility model: slots are respectively opened at both ends of the support plate, and the bottom outer walls of the first annular electrode sheet and the second annular electrode sheet are respectively inserted into the inner wall of the slot.

[0012] The beneficial effects of this utility model are as follows:

[0013] 1. This invention enables the electric field generating device to simultaneously apply electric fields of corresponding intensities to multiple culture dishes, thereby improving the efficiency of cell electric field experiments. At the same time, by setting two radially symmetrical ring electrode configurations, namely the first ring electrode and the second ring electrode, a concentric electric field with decreasing gradient is formed, ensuring the consistency of electric field conditions in symmetrical parts. Compared with traditional flat plate electrodes, this design can better meet the control variable requirements of experimental and control groups.

[0014] 2. The real-time electric field intensity at corresponding positions between the two annular electrode plates is measured using a current probe. The current probe is then moved forward or backward a corresponding distance, and the changes in the measured electric field intensity are observed. This allows for accurate calculation of the electric field intensity at different positions between the first and second annular electrode plates. Researchers can determine the corresponding cell position within the culture dish between the first and second annular electrode plates using a scale, and simultaneously calculate the electric field intensity at that position, improving the accuracy and convenience of acquiring cell electric field experimental data.

[0015] 3. When researchers observe cells inside a culture dish, they can use the scale on one side of the dish to determine the distance between the cells and the second ring electrode, allowing them to clearly determine the corresponding position of the cells between the first and second ring electrodes. Attached Figure Description

[0016] Figure 1 This is a schematic diagram of the main structure of an electric field generating device proposed in this utility model;

[0017] Figure 2 This is a schematic diagram of the transparent partition structure of an electric field generating device proposed in this utility model;

[0018] Figure 3This is a schematic diagram of the bottom structure of the transparent partition of an electric field generating device proposed in this utility model;

[0019] Figure 4 This is a schematic diagram of the slot structure of an electric field generating device proposed in this utility model;

[0020] Figure 5 This is an exploded schematic diagram of the transparent partition structure of an electric field generating device proposed in this utility model.

[0021] In the diagram: 1-Transparent partition, 2-Cultural dish, 3-First annular electrode, 4-Second annular electrode, 5-Wire connector, 6-Signal generator, 7-Support plate, 8-Scale, 9-Current probe, 10-Connecting wire, 11-Main unit, 12-Wire hole, 13-Card slot, 14-Slot. Detailed Implementation

[0022] The technical solution of this patent will be further described in detail below with reference to specific embodiments.

[0023] The embodiments of this patent are described in detail below. Examples of these embodiments are shown in the accompanying drawings, wherein the same or similar reference numerals denote the same or similar elements or elements having the same or similar functions throughout. The embodiments described below with reference to the accompanying drawings are exemplary and are only used to explain this patent, and should not be construed as limiting this patent.

[0024] In the description of this patent, it should be understood that the terms “center,” “upper,” “lower,” “front,” “back,” “left,” “right,” “vertical,” “horizontal,” “top,” “bottom,” “inner,” and “outer,” etc., indicate the orientation or positional relationship based on the orientation or positional relationship shown in the accompanying drawings. They are used only for the convenience of describing this patent and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation. Therefore, they should not be construed as limitations on this patent.

[0025] In the description of this patent, it should be noted that, unless otherwise explicitly specified and limited, the terms "installation," "connection," "linking," and "setting" should be interpreted broadly. For example, they can refer to a fixed connection or setting, a detachable connection or setting, or an integral connection or setting. Those skilled in the art can understand the specific meaning of the above terms in this patent according to the specific circumstances.

[0026] Example 1

[0027] An electric field generating device, such as Figures 1-5As shown, the device includes a transparent partition 1. A first annular electrode 3 is provided on the outer wall of the transparent partition 1, and a second annular electrode 4 is provided on the inner wall of the transparent partition 1. The transparent partition 1 is located at the middle position in the vertical direction between the first annular electrode 3 and the second annular electrode 4. Multiple slots 13 are opened on the top outer wall of the transparent partition 1. A culture dish 2 is inserted into the inner wall of the slots 13. Electrical wire connectors 5 are respectively provided on the outer walls of the first annular electrode 3 and the second annular electrode 4. The electrical wire connectors 5 are connected to a signal generator 6 through wire lines.

[0028] The outer wall of the transparent partition 1 is provided with multiple scales 8 corresponding to the slots 13;

[0029] The outer wall of the first annular electrode sheet 3 has a wire hole 12, and a connecting wire 10 is inserted into the inner wall of the wire hole 12.

[0030] One end of the connecting line 10 is connected to a current probe 9, and the other end of the connecting line 10 is connected to a host 11. The current probe 9 is electrically connected to the host 11 through the connecting line 10.

[0031] Researchers first inserted multiple culture dishes 2 containing corresponding cells into the corresponding slots 13 on the outer wall of the transparent partition 1. Then, they connected the wire connectors 5 on the outer walls of the first annular electrode 3 and the second annular electrode 4 to the signal generator 6 via conductive lines. After setting a specific value in the signal generator 6, the current was transmitted to the first annular electrode 3 and the second annular electrode 4 through the wires, thereby generating an electric field of a specific direction and magnitude in the multiple culture dishes 2 placed on top of the transparent partition 1. Researchers could then observe the corresponding culture dishes 2 after being subjected to the electric field using a microscope. This allowed the electric field generating device to simultaneously apply electric fields of corresponding strengths to multiple culture dishes 2, improving the efficiency of cell electric field experiments.

[0032] Meanwhile, by setting up two radially symmetrical annular electrode configurations, the first annular electrode plate 3 and the second annular electrode plate 4, a concentric circular electric field with decreasing gradient is formed, ensuring the consistency of the electric field conditions in the symmetrical parts. Compared with traditional flat plate electrodes, this design can better meet the control variable requirements of the experimental group and the control group.

[0033] When researchers observe the cells inside the corresponding culture dish 2, they can use the scale 8 on one side of the culture dish 2 to understand the distance between the cells inside the culture dish 2 and the second ring electrode 4, allowing researchers to clearly determine the corresponding position of the cells between the first ring electrode 3 and the second ring electrode 4.

[0034] Researchers can move the current probe 9 back and forth on the top of the transparent partition 1 by pushing the connecting wire 10. Then, they can determine the corresponding position of the current probe 9 between the first annular electrode 3 and the second annular electrode 4 by using the scale 8 on one side of the current probe 9. The real-time electric field intensity at the corresponding position is measured by the current probe 9. Then, the current probe 9 is pushed forward or backward a corresponding distance, and the change in the value of the electric field intensity measured by the current probe 9 is observed. This allows for the accurate calculation of the electric field intensity values ​​at different positions between the second annular electrode 4 and the first annular electrode 3. This enables researchers to determine the corresponding position of the cells inside the culture dish 2 between the first annular electrode 3 and the second annular electrode 4 by using the scale 8, and to calculate the electric field intensity value of the cells at that time based on the corresponding position data, thereby improving the accuracy and convenience of obtaining cell electric field experimental data.

[0035] In this embodiment, the transparent partition 1 is made of transparent plastic, the current probe consisting of the host 11, the connecting wire 10 and the current probe 9 is model CP9012A, and the signal generator 6 is an existing signal generator that can be purchased on the market.

[0036] Working principle: First, multiple culture dishes 2 containing corresponding cells are sequentially inserted into the corresponding slots 13 on the outer wall of the transparent partition 1. The electrical wire connectors 5 on the outer walls of the first annular electrode 3 and the second annular electrode 4 are connected to the signal generator 6 through conductive lines. Then, a specific value is set through the signal generator 6, and the current is transmitted to the first annular electrode 3 and the second annular electrode 4 through the electrical wires, generating an electric field of a specific direction and magnitude in the multiple culture dishes 2 placed on top of the transparent partition 1. The corresponding culture dishes 2 after being affected by the electric field can then be observed through a microscope.

[0037] The distance between the cells inside the culture dish 2 and the second ring electrode 4 can be determined by the scale 8 on one side of the culture dish 2.

[0038] The current probe 9 can be moved back and forth on the top of the transparent partition 1 by pushing the connecting wire 10. Then, the corresponding position of the current probe 9 between the first annular electrode 3 and the second annular electrode 4 can be determined by the scale 8 on one side of the current probe 9. The real-time electric field intensity at the corresponding position can be measured by the current probe 9. Then, the current probe 9 can be moved forward or backward by the corresponding distance, and the change in the value of the electric field intensity measured by the current probe 9 can be observed. The electric field intensity values ​​at different positions between the second annular electrode 4 and the first annular electrode 3 can be accurately calculated. This allows researchers to determine the corresponding position of the corresponding cell inside the culture dish 2 between the first annular electrode 3 and the second annular electrode 4 according to the scale 8, and at the same time, calculate the electric field intensity value of the cell at this time based on the corresponding position data.

[0039] Example 2

[0040] An electric field generating device, such as Figures 1-5 As shown, this embodiment makes the following improvements based on embodiment 1: multiple support plates 7 are fixed to the bottom outer wall of the transparent partition 1;

[0041] The support plate 7 has slots 14 at both ends, and the bottom outer walls of the first annular electrode 3 and the second annular electrode 4 are respectively inserted into the inner wall of the slot 14.

[0042] The support plate 7 can support the transparent partition 1, the first annular electrode 3 and the second annular electrode 4 on the top of the transparent partition 1, and multiple culture dishes 2. At the same time, the two slots 14 located on the top of the support plate 7 can clamp, fix and limit the second annular electrode 4 and the first annular electrode 3 inserted into the inner and outer walls of the transparent partition 1, improve the stability of the connection between the second annular electrode 4 and the first annular electrode 3 and the transparent partition 1, and facilitate researchers to disassemble and replace the first annular electrode 3 and the second annular electrode 4.

[0043] Working principle: The support plate 7 can support the transparent partition 1, the first annular electrode 3 and the second annular electrode 4 on the top of the transparent partition 1, and multiple culture dishes 2. At the same time, the two slots 14 located on the top of the support plate 7 can clamp, fix and limit the second annular electrode 4 and the first annular electrode 3 inserted into the inner and outer walls of the transparent partition 1.

[0044] The above description is only a preferred embodiment of the present utility model, but the protection scope of the present utility model is not limited thereto. Any equivalent substitutions or changes made by those skilled in the art within the technical scope disclosed in the present utility model, based on the technical solution and the inventive concept of the present utility model, should be included within the protection scope of the present utility model.

Claims

1. An electric field generating device, comprising a transparent partition (1), characterized in that, The outer wall of the transparent partition (1) is provided with a first annular electrode plate (3), and the inner wall of the transparent partition (1) is provided with a second annular electrode plate (4). The transparent partition (1) is located at the middle position in the vertical direction between the first annular electrode plate (3) and the second annular electrode plate (4). The top outer wall of the transparent partition (1) has multiple slots (13). A petri dish (2) is inserted into the inner wall of the slots (13). The outer walls of the first annular electrode plate (3) and the second annular electrode plate (4) are respectively provided with electrical wire connectors (5). The electrical wire connectors (5) are connected to a signal generator (6) through wire lines.

2. The electric field generating device according to claim 1, characterized in that, The outer wall of the transparent partition (1) is provided with multiple scales (8) corresponding to the slots (13).

3. The electric field generating device according to claim 1, characterized in that, The outer wall of the first annular electrode sheet (3) has a through hole (12), and a connecting wire (10) is inserted into the inner wall of the through hole (12).

4. An electric field generating device according to claim 3, characterized in that, One end of the connecting line (10) is connected to a current probe (9), and the other end of the connecting line (10) is connected to a host (11). The current probe (9) is electrically connected to the host (11) through the connecting line (10).

5. An electric field generating device according to claim 2, characterized in that, The bottom outer wall of the transparent partition (1) is fixed with multiple support plates (7).

6. An electric field generating device according to claim 5, characterized in that, The support plate (7) has slots (14) at both ends, and the bottom outer walls of the first annular electrode plate (3) and the second annular electrode plate (4) are respectively inserted into the inner wall of the slot (14).

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