Cell culture bag magnetic attraction limiting position clamp

Abstract

The utility model provides a kind of cell culture bag magnetic attraction position limiting clamp, it is related to biotechnology equipment field, including position limiting clamp monomer, position limiting clamp monomer includes clamping body, connecting structure, magnet and bottom plate;Clamping body is cuboid structure, clamping body both ends are provided with connecting structure, magnet is arranged in clamping body interior;Bottom plate is arranged in the clamping surface of clamping body;Connecting structure includes aperture and pivot, one end of clamping body is equipped with aperture, the other end is equipped with pivot matched with aperture.The utility model realizes a set of tool to adapt multiple culture bag specifications by modularization design, effectively reduces consumable usage, reduces culture cost;Avoid frequent transfer operation, significantly reduce cell pollution risk;The function of calculating limited area provides reproducible standard data for cell culture process.

Description

Technical Field

[0001] This utility model relates to the field of biotechnology equipment, specifically to a magnetic clamp for cell culture bags. Background Technology

[0002] Currently, conventional cell culture expansion protocols typically start with smaller culture flasks and gradually expand the culture system by replacing them with larger flasks of different sizes. A typical culture flow is: T25 culture flask → T75 culture flask → T175 culture flask → T225 culture flask → large-volume cell culture bag. In the initial stage of cell transfer to the cell culture bag, because the number of cells is relatively small, the required amount of culture medium is also correspondingly small. Therefore, it is necessary to reduce the surface area used for cell culture by folding part of the bag to maintain an appropriate cell density.

[0003] However, the aforementioned traditional culture protocols have several technical problems. First, frequent cell transfer operations increase the risk of cell contamination. Each transfer requires complex procedures under sterile conditions, and any operational error can lead to the failure of the entire culture process. Second, the use of a large number of consumables results in high culture costs. Different sizes of culture containers need to be purchased and stored separately, increasing laboratory operating costs. Furthermore, during the use of cell culture bags, there is a lack of effective methods for controlling the culture area. Traditional folding methods are difficult to achieve accurate area adjustment, and the folding operation may damage the culture bags.

[0004] Existing methods for controlling cell culture area mainly rely on manually folding culture bags or using simple clamps. These methods are insufficient in terms of accuracy, stability, and ease of operation. Manual folding cannot provide accurate area control and is easily affected by improper operation, thus impacting culture results. While simple clamps can fix the folding position, they lack area measurement capabilities and are usually designed for fixed sizes, making it difficult to adapt to the needs of culture bags of different sizes.

[0005] Chinese patent document CN207227453U discloses a magnetic limiting clip for cell culture bags, which discloses a technical solution including a soft magnetic strip and a buckle. The soft magnetic strip is folded and magnetically attracted to achieve flexible adjustment of the amount of culture medium in the culture bag, which has the technical effect of reducing the probability of contamination and facilitating operation. However, it still has the problems of not being able to accurately calculate and control the culture area, lacking modular expansion capability, and not providing sufficient protection for the culture bag.

[0006] Chinese patent document CN203889256U discloses an infinitely stackable sealing clip, which discloses a technical solution including at least two sealing clips that can be infinitely stacked. The sealing clips achieve simultaneous sealing of multiple bag openings by cooperating with the openings and beveled protrusions, which improves sealing efficiency and enhances durability. However, it still has the problems of being a general sealing tool rather than being specifically designed for cell culture applications, lacking area control and calculation functions, and failing to meet the professional requirements of cell culture. Utility Model Content

[0007] The purpose of this invention is to provide a magnetic clamp for cell culture bags that can precisely control the culture area, reduce the risk of contamination, reduce the amount of consumables used, and is easy to operate.

[0008] To achieve the above objectives, this utility model employs the following technical solution:

[0009] A magnetic retaining clip for a cell culture bag includes a retaining clip unit, wherein the retaining clip unit includes a clip body, a connecting structure, a magnet, and a base plate;

[0010] The clamping body has a cuboid structure, and connecting structures are provided at both ends of the clamping body. The magnet is disposed inside the clamping body. The base plate is disposed on the clamping surface of the clamping body.

[0011] The limiting clamp unit achieves connection between multiple units through the connecting structure, and the limiting clamp unit and the corresponding limiting clamp unit are clamped by the magnet.

[0012] Furthermore, the connecting structure includes an opening and a rotating shaft. One end of the clamp is provided with an opening, and the other end is provided with a rotating shaft that mates with the opening.

[0013] Furthermore, the two ends of the clamp are semi-circular in shape.

[0014] Furthermore, the clamp body is provided with a scale.

[0015] Furthermore: both ends of the clamp are provided with bosses, including a first boss and a second boss. The first boss is a boss with a missing lower part, and the opening is provided at the center of the first boss. The second boss is a boss with a missing upper part, and the center of the second boss is provided with an upwardly extending pivot.

[0016] Furthermore, the rotating shaft has a cylindrical structure, and the opening is a circular hole that matches the rotating shaft.

[0017] Furthermore, the scale is set on the surface of one side of the opening.

[0018] Furthermore, the base plate is made of materials including plastic and silicone rubber.

[0019] Furthermore, the bottom surface of the base plate is flocked.

[0020] Furthermore, the length of the clamp body ranges from 3cm to 10cm.

[0021] Compared with the prior art, the present invention has the following advantages:

[0022] I. This invention solves the contamination risk problem caused by frequent changes in culture containers in traditional cell culture. Utilizing a dual connection mechanism of magnetic attraction and mechanical cooperation, it eliminates the need for complex transfer operations during cell culture, allowing for direct area control at different stages within the same culture bag, significantly reducing the probability of cell contamination.

[0023] Second, by increasing or decreasing the number of limiting clamp units, this utility model can flexibly adjust the total length of the limiting clamp, adapting to the needs of culture bags of various sizes from small to large, greatly reducing the amount of consumables used and effectively controlling culture costs.

[0024] Third, the scale setting of this utility model provides the function of determining and limiting the culture area. Operators can intuitively determine the limited area of ​​the culture bag according to the scale, realizing standardized management of culture conditions. Compared with the traditional manual folding method, scale control makes the setting of the culture area more accurate and provides reproducible reference data.

[0025] IV. The base plate of this utility model is designed with soft materials, especially the flocked surface, which can gently contact the culture bag without causing scratches or other physical damage. The flocked surface increases the friction between the culture bag and the plate, improving the stability of the clamping and effectively dispersing the clamping force, thus avoiding damage to the culture bag that may be caused by localized stress concentration. Attached Figure Description

[0026] Figure 1 A schematic diagram of the clamping state of a magnetic limiting clip for a cell culture bag provided by this utility model;

[0027] Figure 2 An exploded view of a magnetic retaining clip for a cell culture bag provided by this utility model;

[0028] Figure 3 A schematic diagram showing the clamping state of a cell culture bag defined by a magnetic retaining clip over a large area;

[0029] Figure 4 for Figure 3 A partially enlarged schematic diagram of the magnetic retaining clip connection part of the cell culture bag;

[0030] Figure 5 A schematic diagram showing the clamping state of a cell culture bag with a small area defined by a magnetic retaining clip;

[0031] Figure 6 for Figure 5 A partially enlarged schematic diagram of the magnetic retaining clip connection part of the cell culture bag;

[0032] In the picture:

[0033] 1. Clamping body; 2. Magnet; 3. Base plate; 4. Opening; 5. Rotating shaft; 6. Scale; 11. First boss; 12. Second boss. Detailed Implementation

[0034] The technical solution of this utility model will now be clearly and completely described with reference to the accompanying drawings. Obviously, the described embodiments are only some, not all, of the embodiments of this utility model. Based on the embodiments of this utility model, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of this utility model.

[0035] In the description of this utility model, it should be noted that the terms "center," "upper," "lower," "left," "right," "vertical," "horizontal," "inner," and "outer," etc., indicating the orientation or positional relationship, are based on the orientation or positional relationship shown in the accompanying drawings and are only for the convenience of describing this utility model and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation, and therefore should not be construed as a limitation of this utility model. Furthermore, the terms "first," "second," and "third" are used for descriptive purposes only and should not be construed as indicating or implying relative importance.

[0036] It should be noted that the "clamping" of the limiting clamp unit mentioned in this utility model is vertical clamping, with the cell culture bag to be clamped placed in the middle; the "connection" of the limiting clamp unit mentioned in this utility model is horizontal connection, that is, multiple units are connected end to end in the horizontal direction.

[0037] like Figure 1-6 As shown, this utility model provides a magnetic clamping device for cell culture bags, including a clamping unit. The clamping unit includes a clamp body 1, a connecting structure, a magnet 2, and a base plate 3. The clamp body 1 has a cuboid structure, and the clamping units are provided with connecting structures at both ends. The magnet 2 is disposed inside the clamping unit. The base plate 3 is disposed on the clamping surface of the clamping unit. The clamping unit connects multiple units through the connecting structure, and the clamping unit and the corresponding clamping unit are clamped by the magnet 2.

[0038] Specifically, clamp 1 adopts a cuboid structure design, possessing good mechanical strength and capable of withstanding the stress generated during clamping. Both ends of clamp 1 are equipped with connecting structures, allowing multiple clamping units to connect and form an extendable clamping assembly. Magnet 2 is located inside clamp 1, using magnetic attraction to tightly bind two opposing clamping units together, achieving effective clamping of the cell culture bag. Base plate 3 is located on the clamping surface of clamp 1, i.e., the surface in direct contact with the cell culture bag, providing protection. The design of the connecting structure allows for flexible combination of individual clamping units to adapt to the needs of cell culture bags of different sizes. The magnetic attraction of magnet 2 ensures the stability and reliability of clamping. The base plate 3 effectively avoids potential damage caused by direct contact between clamp 1 and the culture bag, protecting the integrity of the culture bag.

[0039] In one specific embodiment of this utility model, the connecting structure includes an opening 4 and a rotating shaft 5. One end of the clamping body 1 has the opening 4, and the other end has the rotating shaft 5 that mates with the opening 4. Specifically, the opening 4 is located at one end of the clamping body 1, forming a space to accommodate the rotating shaft 5. The rotating shaft 5 is located at the other end of the clamping body 1 and has a shape and size that matches the opening 4. When two limiting clamping units need to be connected, the rotating shaft 5 of one unit is inserted into the opening 4 of the other unit to achieve a mechanical connection between the two units. The mating design of the rotating shaft 5 and the opening 4 ensures the accuracy of the connection between the limiting clamping units, improves stability, and avoids misalignment or loosening during the connection process.

[0040] In one specific embodiment of this utility model, the two ends of the clamp 1 are semi-circular. Specifically, the semi-circular design at both ends of the clamp 1 is not only aesthetically pleasing, but more importantly, it reduces sharp edges, preventing accidental damage or scratches to the cell culture bag or the operator. The semi-circular design also provides a better grip, facilitating installation and adjustment by the operator.

[0041] In one specific embodiment of this utility model, the clamp 1 is provided with a scale 6. Specifically, the scale 6 is printed on the surface of the clamp 1, providing an accurate measurement reference. The scale 6 uses clear numbers and engravings for easy reading by operators. The spacing of the scale is designed to meet the actual needs of cell culture and can satisfy the control requirements of different culture areas. The scale 6 enables operators to calculate and control the limited usable area of ​​the culture bag according to the specific needs of cell culture, achieving standardized and repeatable culture condition settings.

[0042] In one specific embodiment of this utility model, the clamping body 1 has bosses at both ends, including a first boss 11 and a second boss 12. The first boss 11 is a boss with a missing lower part, and an opening 4 is located at the center of the first boss 11. The second boss 12 is a boss with a missing upper part, and a rotating shaft 5 extending upward is provided at the center of the second boss 12. Specifically, the first boss 11 adopts a structure with a missing lower part, that is, the lower half of the boss is cut off to form a structure with a protruding upper part. The opening 4 is located at the center of the first boss 11. The second boss 12 adopts a structure with a missing upper part, that is, the upper half of the boss is cut off to form a structure with a protruding lower part. The rotating shaft 5 extends upward from the center of the second boss 12, corresponding to the opening 4. The complementary boss design allows the two limiting clamping bodies to fit tightly together, forming a stable connection structure, while facilitating quick assembly and disassembly.

[0043] In one specific embodiment of this utility model, the rotating shaft 5 has a cylindrical structure, and the opening 4 is a circular hole that matches the rotating shaft 5. Specifically, the rotating shaft 5 adopts a cylindrical structure with a uniform diameter and a smooth surface to ensure smooth insertion and rotation in the opening 4. The opening 4 is a circular hole that matches the diameter of the rotating shaft 5. The design of the circular opening 4 and the cylindrical rotating shaft 5 takes into account both the convenience of rotation and the stability of the connection, ensuring that it is neither too loose, leading to unstable connection, nor too tight, leading to assembly difficulties. Figures 5-6 As shown, when rotating the monomer in the connected state on the culture bag according to actual needs, the design of the circular opening 4 and the cylindrical rotating shaft 5 provides good rotation convenience while ensuring connection stability. It is very convenient for operators to adjust the connection direction and angle between the limit clamps.

[0044] In one specific embodiment of this utility model, the scale 6 is disposed on the surface of one side of the opening 4. Specifically, the scale 6 is printed on the surface of the clamp 1 near the opening 4. The setting of the scale 6 makes it easy for the operator to read clearly from different angles, facilitating the calculation of the usable area limited by the limiting clamp.

[0045] In one specific embodiment of this utility model, the base plate 3 is made of plastic and silicone rubber. Specifically, the base plate 3 can be made of plastic, which has good chemical stability and biocompatibility and will not adversely affect cell culture. Silicone rubber has excellent elasticity and softness, which can better conform to the surface of the culture bag and provide gentler protection. The choice of materials ensures that the base plate 3 will not cause scratches or other physical damage to the culture bag during clamping, thus protecting the culture bag.

[0046] In one specific embodiment of this invention, the bottom surface of the base plate 3 is flocked. Specifically, the flocking process involves attaching short, dense fibrous material to the bottom surface of the base plate 3, forming a surface structure similar to fluff. The flocked surface has a soft touch and good cushioning properties, allowing for gentler contact with the culture bag surface. The flocking process increases the friction between the base plate 3 and the culture bag, improving clamping stability and preventing the culture bag from slipping during clamping. The flocked surface effectively disperses clamping forces, avoiding localized stress concentration that could damage the culture bag.

[0047] In one specific embodiment of this utility model, the length of the clamp 1 ranges from 3cm to 10cm. Specifically, the design of the length of the clamp 1 fully considers the usage requirements of cell culture bags of different sizes. The minimum length of 3cm can meet the clamping requirements of small culture bags and is suitable for the initial stage of cell culture. The maximum length of 10cm can provide a larger clamping range and is suitable for area control of large culture bags. The length range design makes the single limiting clamp unit have good versatility and can adapt to various culture bag sizes. The reasonable design of the length of the clamp 1 ensures the uniform distribution of clamping force.

[0048] The working principle of this utility model is as follows:

[0049] When the area of ​​a cell culture bag needs to be limited, multiple limiting clamp units are first connected according to the size requirements of the culture bag by means of a rotating shaft and an opening, forming a limiting clamp structure of the required length. During the connection process, the rotating shaft of one unit is inserted into the opening of another unit to ensure the stability of the connection.

[0050] In actual use, operators refer to the scale on the clamp to determine the limited usable area of ​​the cell culture bag. The assembled limiting clamp unit is placed in the predetermined position on the culture bag, ensuring the base plate contacts the surface of the culture bag. The soft material or flocked surface of the base plate gently conforms to the culture bag, avoiding scratches or other physical damage.

[0051] When the two opposing clamping units approach each other, the built-in magnets activate. The magnetic attraction generated by the magnets causes the two clamping units to attract each other and fit tightly together, effectively holding the culture bag while ensuring stability and preventing excessive pressure on the culture bag.

[0052] After clamping, the culture bag is divided into different areas, accurately defining the usable area. The cell culture medium is distributed only within the defined area, creating suitable cell density and culture conditions. When it is necessary to adjust the culture area, this can be achieved by repositioning the limiting clamps or increasing or decreasing the number of limiting clamp units.

[0053] This invention effectively solves the problem of frequent replacement of culture containers of different sizes in traditional cell culture, significantly reducing the risk of cell contamination. The modular design allows one set of limiting clamps to adapt to various culture bag sizes, greatly reducing consumable usage and lowering culture costs. The graduation system provides the function of calculating the defined area, providing reproducible standard data for the cell culture process; the soft base plate and flocking treatment protect the integrity of the culture bag, avoiding culture failure due to mechanical damage, and further ensuring the success rate of cell culture.

[0054] The above embodiments are only for illustrating the technical concept and features of this utility model, and are intended to enable those skilled in the art to understand the content of this utility model and implement it accordingly. They should not be construed as limiting the scope of protection of this utility model. All equivalent transformations or modifications made in accordance with the spirit and essence of this utility model should be included within the scope of protection of this utility model.

Claims

1. A magnetic retaining clip for a cell culture bag, characterized in that: The device includes a limiting clamp unit, which comprises a clamp body, a connecting structure, a magnet, and a base plate. The clamping body is elongated and has connecting structures at both ends. The magnet is disposed inside the clamping body. The base plate is disposed on the clamping surface of the clamping body. The limiting clamp unit achieves the connection between multiple limiting clamp units through the connecting structure, and the limiting clamp unit clamps each other with another limiting clamp unit through the magnet.

2. The magnetic clamp for cell culture bags according to claim 1, characterized in that: The connection structure includes an opening and a rotating shaft. One end of the clamp is provided with an opening, and the other end is provided with a rotating shaft that mates with the opening.

3. The magnetic clamp for cell culture bags according to claim 1, characterized in that: The two ends of the clamp are semi-circular in shape.

4. The magnetic clamp for cell culture bags according to claim 2, characterized in that: The clamp is equipped with a scale.

5. The magnetic clamp for cell culture bags according to claim 2, characterized in that: The clamp body has protrusions at both ends, including a first protrusion and a second protrusion. The first protrusion is a protrusion missing at the bottom, and the opening is located at the center of the first protrusion. The second protrusion is a protrusion missing at the top, and the center of the second protrusion has an upwardly extending pivot.

6. The magnetic clamp for cell culture bags according to claim 2, characterized in that: The rotating shaft has a cylindrical structure, and the opening is a circular hole that matches the rotating shaft.

7. A magnetic clamp for cell culture bags according to claim 4, characterized in that: The scale is set on the surface of one side of the opening.

8. The magnetic clamp for cell culture bags according to claim 1, characterized in that: The base plate is made of materials including plastic and silicone rubber.

9. The magnetic clamp for cell culture bags according to claim 1, characterized in that: The bottom surface of the base plate is flocked.

10. A magnetic clamp for cell culture bags according to claim 1, characterized in that: The length of the clamp body ranges from 3cm to 10cm.

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