A plant cell culture vessel
By designing a plant cell culture container with a transparent sealing cap, replaceable air filter membrane, and stirring mechanism, the problems of poor sealing, inconvenient observation, and cumbersome operation have been solved, thus ensuring a sterile environment and improving culture efficiency.
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- XINSHENGYUANCHUANG (XIAMEN) BIOTECHNOLOGY CO LTD
- Filing Date
- 2025-08-12
- Publication Date
- 2026-07-14
AI Technical Summary
Existing plant cell culture containers have poor sealing, making them susceptible to invasion by external bacteria, inconvenient for observation, cumbersome for sampling and replenishment, and lack effective filtration components during aeration, which can easily lead to contamination risks.
A plant cell culture container was designed, comprising a transparent sealing cap, a vent with a replaceable air filter membrane, a liquid replenishment port with a sealing plug, a sampling tube, and a stirring mechanism. It is equipped with temperature and pH sensors and a display screen, has good sealing and observation capabilities, simplifies sampling and liquid replenishment operations, and ensures a sterile environment through the air filter membrane.
It effectively prevents the entry of external bacteria, simplifies sampling and replenishment operations, ensures the sterility of the culture environment, improves culture efficiency and quality, provides suitable light conditions, and ensures uniform mixing of the culture medium.
Smart Images
Figure CN224494225U_ABST
Abstract
Description
Technical Field
[0001] This utility model relates to the field of biological culture equipment, and more specifically, to a plant cell culture container. Background Technology
[0002] Plant cell culture refers to the technique of culturing plant cells on artificially prepared culture media under in vitro conditions to enable their growth, proliferation, and differentiation. During plant cell culture, the performance of the culture vessel has a significant impact on the culture results.
[0003] Currently, existing plant cell culture containers have some shortcomings. For example, some containers have poor sealing, which can easily allow external bacteria to enter and affect the growth of plant cells; some containers make it difficult to observe the culture of plant cells inside, making it difficult to keep track of the culture progress in a timely manner; moreover, sampling, testing, and liquid replenishment are cumbersome and can easily damage the culture environment; at the same time, the lack of effective filtration or the difficulty in replacing filter components during aeration can easily lead to the risk of contamination. Utility Model Content
[0004] This invention provides a plant cell culture container to solve the problems mentioned in the background art.
[0005] To achieve the above objectives, the present invention adopts the following technical solution: a plant cell culture container, comprising a culture tank body and a sealing cap, the sealing cap being made of transparent material and positioned over the opening of the culture tank body, the culture tank body being sealed to the sealing cap, the sealing cap having several vents and liquid inlets penetrating its upper and lower ends, a replaceable air filter membrane being provided at the vent, the air filter membrane being detachably connected to the inner wall of the vent via a snap-fit structure, a sealing plug being provided at the liquid inlet, the sealing plug being interference-fitted with the liquid inlet, a sampling tube being provided on the side wall of the culture tank body, one end of the sampling tube being connected to the cavity of the culture tank body, the other end of the sampling tube being connected to the external space, a valve being provided on the sampling tube, and a stirring mechanism and a light-illuminating mechanism being provided in the cavity of the culture tank body.
[0006] Furthermore, a temperature sensor and a pH sensor are provided on the inner wall of the culture tank body, and a display screen is provided on the outer wall of the culture tank body. Both the temperature sensor and the pH sensor are electrically connected to the display screen.
[0007] Furthermore, the inner wall of the vent is provided with several positioning blocks.
[0008] Furthermore, the stirring mechanism includes a motor, a stirring shaft, and stirring blades. The motor is fixedly mounted on the sealing cover, the top end of the stirring shaft is connected to the output end of the motor, the bottom end of the stirring shaft extends to the cavity of the culture tank body, and the stirring blades are fixedly mounted on the outer wall of the stirring shaft.
[0009] Furthermore, the culture tank body is provided with a partition plate, and the culture tank body has a plurality of limiting grooves. The partition plate is inserted into the limiting grooves, and the partition plate is detachably connected to the culture tank body.
[0010] Furthermore, there are two of each of the liquid replenishment port and the air vent.
[0011] Furthermore, the sealing cap is made of glass, and the main body of the culture tank is made of stainless steel.
[0012] Furthermore, the illumination mechanism includes a limiting block and an LED light. The limiting block is fixedly installed on the inner wall of the culture tank body, and the LED light is installed on the limiting block.
[0013] Furthermore, the air filter membrane is made of polytetrafluoroethylene.
[0014] Furthermore, the top of the vent is provided with an end cap, which is threadedly connected to the sealing cap, and the end cap is provided with a vent hole.
[0015] Compared with the prior art, the beneficial effects of this utility model are:
[0016] This invention, through the design of a sealing cap, a sealing plug, and a vent with a replaceable air filter membrane, ensures the airtightness and sterility of the culture tank body from multiple angles, effectively preventing the entry of external bacteria and providing a reliable sterile environment for plant cell culture. The transparent sealing cap allows staff to observe the plant cell culture at any time and keep track of the culture progress. The combined use of the sampling tube and valve makes sampling simple and convenient. The replenishment port is specifically for replenishing nutrient solution, which is easy to operate and reduces damage to the culture environment. The stirring mechanism ensures uniform mixing of the culture medium. The lighting mechanism provides a suitable lighting environment, improving the efficiency and quality of plant cell culture. Attached Figure Description
[0017] To more clearly illustrate the technical solutions of the embodiments of this utility model, the drawings used in the embodiments will be briefly introduced below. It should be understood that the following drawings only show some embodiments of this utility model and should not be regarded as a limitation of the scope. For those skilled in the art, other related drawings can be obtained from these drawings without creative effort.
[0018] Figure 1 This is a schematic diagram of the structure of this utility model;
[0019] Figure 2 This is an exploded view of the present invention;
[0020] Figure 3 yes Figure 2 A magnified view of part A in the diagram.
[0021] Explanation of main component symbols
[0022] 100. Culture tank body; 101. Sampling tube; 102. Valve; 103. Temperature sensor; 104. pH sensor; 105. Display screen; 106. Limiting groove; 107. Partition plate; 110. Stirring mechanism; 111. Motor; 112. Stirring shaft; 113. Stirring blades; 120. Illumination mechanism; 121. Limiting block; 122. LED light;
[0023] 200, Sealing cap; 201, Vent port; 202, Positioning block; 210, End cap; 211, Vent hole; 212, Air filter membrane; 220, Liquid replenishment port; 221, Sealing plug. Detailed Implementation
[0024] To make the objectives, technical solutions, and advantages of the embodiments of this utility model clearer, the technical solutions of the embodiments of this utility model will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only a part of the embodiments of this utility model, not all of them. All other embodiments obtained by those skilled in the art based on the embodiments of this utility model without creative effort are within the scope of protection of this utility model. Therefore, the following detailed description of the embodiments of this utility model provided in the accompanying drawings is not intended to limit the scope of the claimed utility model, but merely represents selected embodiments of this utility model. All other embodiments obtained by those skilled in the art based on the embodiments of this utility model without creative effort are within the scope of protection of this utility model.
[0025] In this utility model, unless otherwise explicitly specified and limited, the terms "installation," "connection," "joining," and "fixing," etc., should be interpreted broadly. For example, they can refer to a fixed connection, a detachable connection, or an integral part; they can refer to a mechanical connection or an electrical connection; they can refer to a direct connection or an indirect connection through an intermediate medium; they can refer to the internal communication of two components or the interaction between two components. Those skilled in the art can understand the specific meaning of the above terms in this utility model according to the specific circumstances.
[0026] In this invention, unless otherwise explicitly specified and limited, "above" or "below" the second feature can include direct contact between the first and second features, or contact between the first and second features through another feature between them. Furthermore, "above," "over," and "on top" of the second feature includes the first feature directly above or diagonally above the second feature, or simply indicates that the first feature is at a higher horizontal level than the second feature. "Below," "below," and "under" the second feature includes the first feature directly below or diagonally below the second feature, or simply indicates that the first feature is at a lower horizontal level than the second feature.
[0027] Example
[0028] like Figures 1-3 As shown, this utility model discloses a plant cell culture container, which includes a culture tank body 100 and a sealing cover 200. The sealing cover 200 is made of transparent material, which facilitates observation of the interior of the culture tank body 100. In this embodiment, the sealing cover 200 is placed over the opening of the culture tank body 100, and the culture tank body 100 and the sealing cover 200 are sealed together to ensure that external bacteria cannot enter the culture tank body 100 and affect the plant cell culture. The sealing cover 200 has several vents 201 and replenishment ports 220 penetrating its upper and lower ends. The vents 201 ensure smooth gas flow. The replenishment ports 220 are used to replenish the culture medium inside the culture tank body 100. A replaceable air filter membrane 212 is provided at the vents 201, which ensures the sterility of the air entering the culture tank body 100. The air filter membrane 212 is secured by a snap-fit structure. The membrane is detachably connected to the inner wall of the vent 201, facilitating periodic membrane replacement to ensure filtration efficiency. A sealing plug 221 is provided at the replenishment port 220, with an interference fit between the plug and the port, ensuring a tight seal when not in use. A sampling tube 101 is located on the side wall of the culture tank body 100. One end of the sampling tube 101 is connected to the cavity of the culture tank body 100, and the other end is connected to the external space. A valve 102 is provided on the sampling tube 101; opening the valve 102 allows sampling of the culture medium inside the culture tank body 100, simplifying operation and minimizing disruption to the culture environment. A stirring mechanism 110 and a lighting mechanism 120 are located in the cavity of the culture tank body 100. The stirring mechanism 110 ensures uniform mixing of the culture medium, promoting plant cell growth. The lighting mechanism 120 provides the necessary light conditions for plant cell growth.
[0029] The inner wall of the culture tank body 100 is equipped with a temperature sensor 103 and a pH sensor 104, and the outer wall of the culture tank body 100 is equipped with a display screen 105. The temperature sensor 103 and the pH sensor 104 are electrically connected to the display screen 105. The temperature sensor 103 and the pH sensor 104 can monitor the temperature and pH value inside the culture tank body 100 in real time and display them on the display screen 105, so that the staff can understand the changes in the culture environment in a timely manner.
[0030] The inner wall of the above-mentioned air vent 201 is provided with several positioning blocks 202. The positioning blocks 202 can be engaged with the air filter membrane 212, which makes it convenient for staff to replace the air filter membrane 212 and also serves to position the air filter membrane 212.
[0031] The aforementioned sealing plug 221 is made of silicone material. Silicone has good elasticity, sealing properties and chemical stability, and is resistant to high temperature and aging. It can adapt well to the environment of plant cell culture. The interference fit between the plug and the infusion port 220 ensures the sealing of the infusion port 220.
[0032] The stirring mechanism 110 includes a motor 111, a stirring shaft 112, and a stirring blade 113. The motor 111 is fixedly mounted on the sealing cover 200. The top end of the stirring shaft 112 is connected to the output end of the motor 111. The bottom of the stirring shaft 112 extends to the cavity of the culture tank body 100. The stirring blade 113 is fixedly mounted on the outer wall of the stirring shaft 112. The stirring mechanism 110 can make the culture medium mix evenly.
[0033] The aforementioned culture tank body 100 is equipped with a partition plate 107. The culture tank body 100 has several limiting grooves 106, and the partition plate 107 is inserted into the limiting grooves 106. The partition plate 107 is detachably connected to the culture tank body 100. The partition plate 107 can divide the cavity of the culture tank body 100 into multiple independent areas. Each area can hold solid culture media of different components or states to meet the diverse culture needs of plant cells. The partition plate 107 is made of elastic silicone material, which fits tightly against the inner wall of the culture tank body 100 to prevent leakage between different areas of culture media. Furthermore, the number and size of the partitions can be flexibly adjusted as needed. A micro-vibrator is also placed in the main body 100 of the culture tank. The vibration frequency of the micro-vibrator is adjustable. The slight vibration of the micro-vibrator can promote the uniform distribution of nutrients in the solid culture medium and prevent the solid culture medium from caking. It should be noted that when using solid culture medium to culture plant cells, the stirring mechanism 110 needs to be turned off in advance to avoid the rotation of the stirring blade 113 from affecting the solid culture medium. In addition, the opening of the sampling tube 101 should also be sealed in advance with a sealing device to prevent solid culture medium from entering the sampling tube 101. This invention is applicable to both culture medium and solid culture medium.
[0034] The bottom of the sealing cover 200 is provided with a sealing gasket, which is made of rubber material, to enhance the sealing between the sealing cover 200 and the culture tank body 100 and prevent the entry of miscellaneous bacteria.
[0035] The above-mentioned liquid replenishment port 220 and vent port 201 are both two in number. The two liquid replenishment ports 220 are symmetrically distributed on the sealing cap 200, and the two vent ports 201 are also symmetrically distributed on the sealing cap 200. This distribution makes the overall structure of the container more balanced and the operation more convenient.
[0036] The aforementioned sealing cap 200 is made of glass. Glass not only has good light transmittance, facilitating observation of the internal conditions, but also possesses good chemical stability and high-temperature resistance, adapting to various environments during plant cell culture. The main body of the culture vessel 100 is made of stainless steel. Stainless steel is chemically stable and will not release harmful substances to the cells during contact with the culture medium and cells, thus not affecting the normal growth and metabolism of plant cells and ensuring the accuracy and reliability of the culture results. In addition, a window is provided on one side of the main body of the culture vessel 100, and high borosilicate glass is sealed at the window using laser welding to form an observation window, facilitating observation of the internal conditions.
[0037] The aforementioned lighting mechanism 120 includes a limiting block 121 and an LED lamp 122. The limiting block 121 is fixedly installed on the inner wall of the culture tank body 100, and the LED lamp 122 is installed on the limiting block 121. The LED lamp 122 can provide the light conditions required for plant cell growth, and the LED lamp 122 has the advantages of low energy consumption and long life.
[0038] The air filter membrane 212 mentioned above is made of polytetrafluoroethylene with a pore size of 0.22μm, which can effectively filter bacteria, microorganisms and other impurities in the air.
[0039] The upper half of the inner wall of the vent 201 is threaded, the top of the vent 201 is provided with an end cap 210, the lower half of the end cap 210 is threaded, the end cap 210 is threaded to the sealing cap 200, the end cap 210 is provided with a vent hole 211, the end cap 210 can protect the air filter membrane 212, and the vent hole 211 can ensure smooth gas flow.
[0040] The dimensions of the main body 100 of the above-mentioned culture tank are 498*398*60mm, and the dimensions of the sealing cover 200 are 500*400*6mm.
[0041] The above are merely preferred embodiments of this utility model and are not intended to limit the scope of this utility model. Various modifications and variations can be made to this utility model by those skilled in the art. Any modifications, equivalent substitutions, or improvements made within the spirit and principles of this utility model should be included within the protection scope of this utility model.
Claims
1. A plant cell culture container, characterized in that, The culture container includes a culture tank body and a sealing cap. The sealing cap is made of transparent material and is placed over the opening of the culture tank body. The culture tank body and the sealing cap are sealed together. The sealing cap has several vents and liquid inlets penetrating its upper and lower ends. Each vent has a replaceable air filter membrane, which is detachably connected to the inner wall of the vent via a snap-fit structure. Each liquid inlet has a sealing plug, which is interference-fitted with the liquid inlet. A sampling tube is provided on the side wall of the culture tank body. One end of the sampling tube is connected to the cavity of the culture tank body, and the other end is connected to the external space. The sampling tube is equipped with a valve. A stirring mechanism and a light irradiation mechanism are provided in the cavity of the culture tank body.
2. The plant cell culture container according to claim 1, characterized in that; A temperature sensor and a pH sensor are provided on the inner wall of the culture tank body, and a display screen is provided on the outer wall of the culture tank body. The temperature sensor and the pH sensor are both electrically connected to the display screen.
3. A plant cell culture container according to claim 1, characterized in that; The inner wall of the vent is provided with several limiting blocks.
4. A plant cell culture container according to claim 1, characterized in that; The stirring mechanism includes a motor, a stirring shaft, and stirring blades. The motor is fixedly mounted on the sealing cover. The top end of the stirring shaft is connected to the output end of the motor. The bottom of the stirring shaft extends to the cavity of the culture tank body. The stirring blades are fixedly mounted on the outer wall of the stirring shaft.
5. A plant cell culture container according to claim 1, characterized in that: The culture tank body is provided with a partition plate, and the culture tank body has a number of limiting grooves. The partition plate is inserted into the limiting grooves and is detachably connected to the culture tank body.
6. A plant cell culture container according to claim 1, characterized in that: There are two liquid inlets and two vents.
7. A plant cell culture container according to claim 1, characterized in that: The sealing cap is made of glass, and the main body of the culture tank is made of stainless steel.
8. A plant cell culture container according to claim 1, characterized in that; The illumination mechanism includes a limiting block and an LED light. The limiting block is fixedly installed on the inner wall of the culture tank body, and the LED light is installed on the limiting block.
9. A plant cell culture container according to claim 1, characterized in that... ； The air filter membrane is made of polytetrafluoroethylene.
10. A plant cell culture container according to claim 1, characterized in that; The vent is provided with an end cap at its top, which is threadedly connected to the sealing cap, and the end cap has a vent hole.