Nanopore single molecule detection device with electromagnetic interference resistance

Abstract

The utility model discloses a kind of nanometer hole single molecule detection devices of anti-electromagnetic interference, including shielding box main body, shielding box cover and pulse source;The shielding box main body with the shielding box cover is movably connected;First clamping seat, second clamping seat, detection cell clamping groove and detection cell are equipped in the shielding box main body;The detection cell clamping groove is clamped between first clamping seat and second clamping seat, and the detection cell is embedded in the detection cell clamping groove;Electrode is equipped in the detection cell, and the electrode is electrically connected with the pulse source.The utility model effectively solves the technical problem that the nanometer hole detection device of prior art in practical application, signal noise is generated due to external electromagnetic interference, leading to low detection sensitivity, low resolution.

Description

Technical Field

[0001] This invention belongs to the field of nanopore detection and analysis technology, and relates to a nanopore single-molecule detection device that is resistant to electromagnetic interference. Background Technology

[0002] Nanopore detection technology, as a cutting-edge single-molecule analysis method, has shown great application potential in life sciences, medical diagnostics, food safety, and environmental monitoring in recent years, especially in gene sequencing, protein dynamics research, and molecular interaction analysis, demonstrating unique advantages. The core principle of this technology is that when a single analyte molecule passes through a nanopore under the influence of an applied electric field, it causes a momentary blockage of the ion current, thereby generating a specific electrical signal, enabling molecule recognition and classification. However, because the ion current generated in nanopores is usually extremely weak, with signal strength often on the order of picoamperes (pA), this places extremely high demands on the sensitivity and anti-interference capabilities of the signal acquisition system. While commonly used transimpedance amplifier circuits can amplify the detection signal to some extent, their anti-electromagnetic interference capabilities are limited, making them highly susceptible to the influence of high-frequency electromagnetic waves from surrounding electrical appliances and wireless devices, causing baseline drift and signal distortion, severely affecting detection accuracy and data repeatability.

[0003] Most existing nanopore detection systems are used in laboratory environments and rely on external shielding devices such as metal covers and Faraday cages. These systems are complex in structure and inconvenient to operate, making them unsuitable for on-site testing or portable device applications. Utility Model Content

[0004] To address the problems existing in the prior art, this utility model provides a nanopore single-molecule detection device that resists electromagnetic interference, thereby solving the technical problem that existing nanopore detection devices suffer from low detection sensitivity and low resolution due to signal noise caused by external electromagnetic interference in practical applications.

[0005] This utility model is achieved through the following technical solution:

[0006] An electromagnetic interference-resistant nanoporous single-molecule detection device includes a shielding box body, a shielding box cover, and a pulse source;

[0007] The shielding box body is movably connected to the shielding box cover.

[0008] The shielding box body is provided with a first card holder, a second card holder, a detection pool slot, and a detection pool;

[0009] The detection pool slot is engaged between the first slot and the second slot, and the detection pool is embedded in the detection pool slot.

[0010] The detection cell is equipped with electrodes, which are electrically connected to the pulse source.

[0011] Preferably, both the first card holder and the second card holder include a first card edge, a second card edge, and a third card edge that are connected in sequence; the first card edge, the second card edge, and the third card edge are all fitted to the outer wall of the detection pool slot.

[0012] Preferably, the second card edge is provided with a first arc-shaped groove.

[0013] Preferably, the detection pool slot includes a first card wall, a second card wall, a third card wall, and a fourth card wall connected in sequence; the inner wall of the first card wall is provided with a second arc-shaped groove, the inner wall of the third card wall is provided with a third arc-shaped groove, and the second arc-shaped groove and the third arc-shaped groove are symmetrically arranged on both sides of the detection pool.

[0014] Preferably, the first card wall is provided with a threaded hole, and a fastener is fitted on the threaded hole; in use, the fastener abuts against the detection pool.

[0015] Preferably, the shielding box body is provided with an electrode socket, and the electrode is connected to the pulse source through the electrode socket.

[0016] Preferably, the shielding box body includes a first sidewall, a second sidewall, a third sidewall, and a fourth sidewall connected in sequence; the connection between the first sidewall, the second sidewall, and the third sidewall is smoothly transitioned.

[0017] Preferably, the fourth sidewall is provided with rounded corner components at the connection points with the first sidewall and the third sidewall.

[0018] Preferably, the shielding box body further includes a base plate, and the base plate is provided with a plurality of fixing holes.

[0019] Preferably, the shielding box cover includes a cover body and a sealing side connected to the cover body.

[0020] Compared with the prior art, the present invention has the following beneficial technical effects:

[0021] This invention discloses an electromagnetic interference-resistant nanoporous single-molecule detection device. The device includes a shielding box body and a shielding box cover, which are movably connected to form a relatively enclosed space. This space shields the internal components, preventing external electromagnetic interference and reducing signal noise. Within the shielding box body, a first retainer, a second retainer, a detection cell slot, and a detection cell are designed. The detection cell slot is positioned between the first and second retainers, and the detection cell is embedded within the slot. This stable nested structure not only ensures the fixed position of the detection cell, reducing interference caused by shaking, but also further enhances the overall anti-interference capability of the device. Simultaneously, electrodes are installed within the detection cell and electrically connected to a pulse source. With the shielding box effectively shielding against external electromagnetic interference, the stable connection between the electrodes and the pulse source ensures the stability and accuracy of signal transmission, preventing signal distortion due to external interference. This improves the sensitivity and resolution of the detection device, providing more reliable and accurate technical support for nanoporous single-molecule detection. Attached Figure Description

[0022] To more clearly illustrate the technical solutions of the embodiments of this utility model, the drawings used in the embodiments will be briefly introduced below. It should be understood that the following drawings only show some embodiments of this utility model and should not be regarded as a limitation on the scope. For those skilled in the art, other related drawings can be obtained based on these drawings without creative effort.

[0023] Figure 1 This is a schematic diagram of the structure of a nanoporous single-molecule detection device that resists electromagnetic interference according to this utility model;

[0024] Figure 2 This is a schematic diagram of the structure of the first card holder and the second card holder in this utility model;

[0025] Figure 3 This is a schematic diagram of the detection pool slot in this utility model;

[0026] Figure 4 This is a schematic diagram of the structure of the bottom plate of the shielding box body in this utility model.

[0027] The components include: 1. Shielding box body; 11. First side wall; 12. Second side wall; 13. Third side wall; 14. Fourth side wall; 15. Rounded corner assembly; 16. Base plate; 17. Fixing hole; 2. Shielding box cover; 21. Cover body; 22. Sealing side; 3. Electrode socket; 31. First mounting bracket; 32. Second mounting bracket; 311. First mounting edge; 312. Second mounting edge; 313. Third mounting edge; 314. First arc groove; 32. Second mounting bracket; 4. Detection cell slot; 41. First mounting wall; 411. Second arc groove; 42. Second mounting wall; 43. Third mounting wall; 431. Third arc groove; 44. Fourth mounting wall; 45. Threaded hole; 5. Detection cell; 51. First detection element; 511. First buffer solution tank; 512. First electrode slot; 513. First aspiration tank; 52. Second detection element. 521. Second buffer solution tank; 522. Second electrode slot; 523. Second aspiration tank. Detailed Implementation

[0028] To make the objectives, technical solutions, and advantages of the embodiments of this utility model clearer, the technical solutions of the embodiments of this utility model will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of this utility model, and not all embodiments. The components of the embodiments of this utility model described and shown in the accompanying drawings can generally be arranged and designed in various different configurations.

[0029] Therefore, the following detailed description of the embodiments of the present invention provided in the accompanying drawings is not intended to limit the scope of the claimed invention, but merely to illustrate selected embodiments of the invention. All other embodiments obtained by those skilled in the art based on the embodiments of the present invention without inventive effort are within the scope of protection of the present invention.

[0030] It should be noted that similar labels and letters in the following figures indicate similar items. Therefore, once an item is defined in one figure, it does not need to be further defined and explained in subsequent figures.

[0031] In the description of the embodiments of this utility model, it should be noted that if terms such as "upper," "lower," "horizontal," or "inner" indicate the orientation or positional relationship based on the orientation or positional relationship shown in the accompanying drawings, or the orientation or positional relationship commonly used when the utility model product is in use, they are only for the convenience of describing the utility model and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation, and therefore should not be construed as a limitation on the utility model. Furthermore, terms such as "first" and "second" are only used to distinguish descriptions and should not be construed as indicating or implying relative importance.

[0032] Furthermore, the use of the term "horizontal" does not imply that the component must be absolutely horizontal, but rather that it can be slightly tilted. For example, "horizontal" simply means that its direction is more horizontal than "vertical," and does not mean that the structure must be completely horizontal, but can be slightly tilted.

[0033] In the description of the embodiments of this utility model, it should also be noted that, unless otherwise explicitly specified and limited, the terms "set," "install," "connect," and "link" should be interpreted broadly. For example, they can refer to a fixed connection, a detachable connection, or an integral connection; they can refer to a mechanical connection or an electrical connection; they can refer to a direct connection or an indirect connection through an intermediate medium; and they can refer to the internal connection of two components. Those skilled in the art can understand the specific meaning of the above terms in this utility model according to the specific circumstances.

[0034] The present invention will now be described in further detail with reference to the accompanying drawings:

[0035] An electromagnetic interference-resistant nanoporous single-molecule detection device includes a shielded box body 1, a shielded box cover 2, and a pulse source. The shielded box body 1 and the shielded box cover 2 are movably connected. The shielded box body 1 and the shielded box cover 2 can be movably connected by hinge or snap-fit, which facilitates the opening and closing of the shielded box cover 2. This allows for easy operation when it is necessary to inspect or replace internal components, and also ensures that a relatively closed and stable environment is formed inside the shielded box during the detection process, effectively reducing the interference of external factors on the detection signal.

[0036] The shielding box body 1 is provided with a first retaining seat 31, a second retaining seat 32, a detection pool slot 4, and a detection pool 5. The first retaining seat 31 and the second retaining seat 32 are the foundation for fixing the detection pool 5, and together they form a space to accommodate a key transition component, namely the detection pool slot 4. The detection pool slot 4 is engaged between the first retaining seat 31 and the second retaining seat 32, and the detection pool 5 is embedded in the detection pool slot 4. This double-layer nested structure, in which the detection pool 5 is placed in the detection pool slot 5 and then engaged between the first retaining seat 31 and the second retaining seat 32, provides multiple layers of stability and precise positioning, effectively isolating the influence of possible external mechanical vibrations on the extremely sensitive nanopore detection process.

[0037] The detection cell 5 contains electrodes that are electrically connected to the pulse source. The electrodes are typically Ag / AgCl electrodes or other suitable electrode materials. These electrodes are electrically connected to an external pulse source via wires to apply the required transmembrane voltage or pulse signal to the detection system.

[0038] The detection cell 5 includes a first detection element 51 and a second detection element 52. The first detection element 51 is provided with a first buffer solution tank 511, a first electrode slot 512, and a first absorption tank 513, which are interconnected. The second detection element 52 is provided with a second buffer solution tank 521, a second electrode slot 522, and a second absorption tank 523, which are interconnected to form a continuous fluid path. The first buffer solution tank 511 and the second buffer solution tank 521 are also interconnected, forming a continuous fluid path. In use, a single-molecule detection membrane is sandwiched at the connection between the first buffer solution tank 511 and the second buffer solution tank 521. That is, when the first detection element 51 and the second detection element 52 are assembled together to form a complete detection cell 5, the first buffer solution tank 511 and the second buffer solution tank 521 are also interconnected at their respective interfaces, and the nanoporous detection membrane is disposed at this connection. In use, the user carefully clamps an extremely thin and precise single-molecule detection membrane (not shown in the diagram), which is the core functional component, at the connection between the first buffer tank 51 and the second buffer tank 521. The nanopores on this membrane become the only channel connecting the two buffer tanks. In this way, the buffer solutions in the first buffer tank 51 and the second buffer tank 521 achieve ion conduction through the nanopores, but are macroscopically separated by the membrane. When a voltage is applied to the electrodes on both sides, an ion current flows through the nanopores. When the analyte molecule passes through the nanopore under the drive of the electric field, it will momentarily block the pore or change the pore characteristics, resulting in a characteristic change in the ion current, i.e., a blocking event, thereby achieving high-sensitivity detection at the single-molecule level.

[0039] The first buffer tank 511 and the second buffer tank 521 are used to hold electrolyte or buffer solution, the first electrode slot 512 and the second electrode slot 522 are used to precisely fix and guide the inserted electrode, which can be an electrode rod or an electrode wire, and the first suction tank 513 and the second suction tank 523 facilitate the emptying of electrolyte or buffer solution.

[0040] In a preferred embodiment, both the first card holder 31 and the second card holder 32 include a first card edge 311, a second card edge 312, and a third card edge 313 connected in sequence; the first card edge 311, the second card edge 312, and the third card edge 313 are all fitted against the outer wall of the detection pool slot 4. Here, the first card edge 311, the second card edge 312, and the third card edge 313 together form a stable "U"-shaped or U-shaped slot structure. In the assembled state, the inner surfaces of the first card edge 311, the second card edge 312, and the third card edge 313 are fitted against the outer wall of the detection pool slot 4. This large-area surface contact provides excellent mechanical stability and vibration resistance, ensuring that the internal core detection module will not be displaced during operation.

[0041] In a further preferred embodiment, the second card edge 312 is provided with a first arc-shaped groove 314. The first arc-shaped groove 314 is designed as the operator's finger or auxiliary tool, such as tweezers, providing a point of force and operating space. This facilitates the removal and insertion of the detection pool card slot 4 from the first card holder 31 and the second card holder 32 when maintenance or replacement is required, greatly improving the accessibility and convenience of operation and avoiding damage to precision components that may be caused by rough operation.

[0042] More preferably, the detection pool slot 4 includes a first retaining wall 41, a second retaining wall 42, a third retaining wall 43, and a fourth retaining wall 44 connected in sequence. The inner wall of the first retaining wall 41 is provided with a second arc-shaped groove 411, and the inner wall of the third retaining wall 43 is provided with a third arc-shaped groove 431. The second arc-shaped groove 411 and the third arc-shaped groove 431 are symmetrically arranged on both sides of the detection pool 5. That is, the first retaining wall 41, the second retaining wall 42, the third retaining wall 43, and the fourth retaining wall 44 enclose a space to accommodate the detection pool 5, namely the detection pool slot 4. A second arc-shaped groove 411 is provided on the inner wall of the first retaining wall 41, i.e., on the side facing the detection pool 5. Symmetrically, a third arc-shaped groove 431 is also provided on the inner wall of the third retaining wall 43. This pair of second arc-shaped grooves 411 and third arc-shaped grooves 431 are symmetrically arranged on both sides of the detection pool 5. The design intent of the arc-shaped grooves here is similar to that of the arc-shaped grooves on the retaining seat, mainly to facilitate the removal and placement of the detection pool 5. When it is necessary to replace the detection membrane or clean the detection pool, users can use the two symmetrical arc grooves as leverage points for their fingers or tools to easily and stably lift or insert the detection pool 5 from the slot 4, reducing the difficulty of operation and the risk of slippage.

[0043] In addition, the first retaining wall 41 is provided with a threaded hole 45, on which a fastener is fitted; in use, the fastener abuts against the detection pool 5. During use, after the detection pool 5 is correctly placed into the detection pool slot 4, the fastener is tightened so that its front end abuts against the side wall of the detection pool 5. This design facilitates the application of a controllable clamping force to the detection pool 5, achieving reliable fixation within the detection pool slot 4, preventing loosening or displacement of the detection pool 5 during the detection process due to liquid flow, electrode connections, or slight external vibrations, thereby ensuring the stability of electrical contact and the reliability of the detection results.

[0044] Furthermore, to simplify the connection between the external pulse source and the internal electrodes of the detection cell 5, improve the speed of experimental setup, and reduce the risk of incorrect connections, an electrode socket 3 is provided on the shielding box body 1. The electrode and the pulse source are connected through the electrode socket 3. The electrode leads inside the detection cell 5 are ultimately connected to this socket, while the output end of the external pulse source is connected to this socket via a cable with a matching plug. In this way, the electrode and the pulse source are connected through the electrode socket 3. This integrated interface design makes the process more convenient. Users do not need to perform tedious wire connection and disconnection inside the shielding box each time. They can simply plug and unplug the external plug to establish and disconnect the electrical path, which greatly improves experimental efficiency, reduces the risk of accidentally touching internal precision components or leads, and helps maintain the cleanliness and shielding integrity inside the shielding box.

[0045] The physical structure of the shielding box body 1 is the basis for its electromagnetic shielding function. It is usually made of a highly conductive metal such as aluminum alloy, copper or galvanized steel plate or conductive composite material. Its main structure includes a first side wall 11, a second side wall 12, a third side wall 13 and a fourth side wall 14 connected in sequence; that is, these four side walls are connected end to end, enclosing the vertical wall part of the shielding box body. In other words, the side walls of the shielding box body 1 are enclosed by the first side wall 11, the second side wall 12, the third side wall 13 and the fourth side wall 14. This closed structure is the basis for forming the Faraday cage effect and shielding external electromagnetic interference.

[0046] In electromagnetic shielding design, avoiding sharp corners and edges ("points") is crucial because points easily accumulate charge, leading to concentrated electric fields or unintended discharge effects. These points can interfere with weak internal electrical signals, reduce shielding effectiveness, and even cause electromagnetic leakage. Therefore, this device pays special attention to this in its structural design: the connections between the first sidewall 11, the second sidewall 12, and the third sidewall 13 are smoothly transitioned. Furthermore, the fourth sidewall 14 is equipped with rounded corner components 15 at its connection with the first and third sidewalls 11 and 13. These rounded corner components 15 can be separately machined and installed arc-shaped parts, or they can be rounded directly at the sidewall connections. The smooth transitions at the connections of the first, second, and third sidewalls 11, 12, and 13, along with the rounded corner components 15, effectively avoid point effects, significantly improve the electromagnetic compatibility performance of the shielding box, ensure that the internal nanopore detection circuit operates in a "clean" electrical environment, reduce background noise, and improve the signal-to-noise ratio of the detection.

[0047] The structural integrity of the shielding box body 1 also depends on its base plate 16. The base plate 16 is typically fixedly connected to the four side walls, such as by welding, screwing, or integral machining, forming a complete box bottom. To securely mount the entire nanopore single-molecule detection device on a laboratory workbench, optical platform, or vibration-damping platform, several fixing holes 17 are provided on the base plate 16. These holes can be through holes for bolts or threaded holes for screws to be screwed in directly. Users can select appropriate bolts, screws, or washers based on the location of the threaded holes or T-slots on the platform, and fix the device to the workbench through these fixing holes 17. This fixing method is particularly important for experiments requiring long-term stable operation, as it effectively isolates external vibrations from being transmitted to the core detection area, further improving the stability and repeatability of the detection.

[0048] Meanwhile, the shielding box cover 2 includes a cover body 21, the shape and size of which match the top opening of the shielding box body 1 to ensure complete coverage. To minimize electromagnetic leakage gaps, the shielding box cover 2 is also designed with a sealing side 22 connected to the cover body 21. This sealing side 22 is designed as a downward-extending flange, the inner wall of which fits tightly against or approaches the outer wall of the top opening of the shielding box body 1 when the cover is closed. A better design is to install conductive elastic gaskets, such as beryllium copper finger springs or conductive rubber strips, at corresponding positions on the sealing side 22 or the shielding box body 1. When the shielding box cover 2 is fastened to the shielding box body 1, the conductive gasket is compressed, forming a continuous, highly conductive contact interface between the cover and the body, significantly reducing the effect of the slot antenna. This design results in better electromagnetic shielding performance, providing the highest level of protection for high-sensitivity electrical detection inside. An observation window, made of metal mesh or conductive glass, can also be provided on the cover body 21 to observe the internal state when needed without frequently opening the cover and disrupting the shielding environment.

[0049] In summary, this nanopore single-molecule detection device, through the shielding box body 1 and shielding box cover 2, the precisely nested fixing structure, namely the first card holder 31 and the second card holder 32, the functional and modular detection cell 5 and its buffer solution and electrode system, the convenient connection interface, namely the electrode socket 3, the optimized anti-sharp structure, namely the smooth transition and rounded corner component 15, and the stable mounting base, namely the base plate 16 and the fixing hole 17, together constructs a highly integrated, easy-to-operate, stable and reliable single-molecule detection platform with excellent electromagnetic shielding performance, providing strong hardware support for the application of nanopore technology in basic research, biosensing and clinical diagnosis.

[0050] The above are merely preferred embodiments of this utility model and are not intended to limit the scope of this utility model. Various modifications and variations can be made to this utility model by those skilled in the art. Any modifications, equivalent substitutions, or improvements made within the spirit and principles of this utility model should be included within the protection scope of this utility model.

Claims

1. A nanoporous single-molecule detection device resistant to electromagnetic interference, characterized in that, It includes the shielding box body (1), the shielding box cover (2), and the pulse source; The shielding box body (1) is movably connected to the shielding box cover (2); The shielding box body (1) is provided with a first card holder (31), a second card holder (32), a detection pool slot (4), and a detection pool (5); The detection pool slot (4) is engaged between the first card holder (31) and the second card holder (32), and the detection pool (5) is embedded in the detection pool slot (4); The detection cell (5) is equipped with electrodes, which are electrically connected to the pulse source.

2. The electromagnetic interference-resistant nanoporous single-molecule detection device according to claim 1, characterized in that, The first card holder (31) and the second card holder (32) each include a first card edge (311), a second card edge (312) and a third card edge (313) connected in sequence; the first card edge (311), the second card edge (312) and the third card edge (313) are all fitted to the outer wall of the detection pool slot (4).

3. The electromagnetic interference-resistant nanoporous single-molecule detection device according to claim 2, characterized in that, The second edge (312) is provided with a first arc-shaped groove (314).

4. The electromagnetic interference-resistant nanoporous single-molecule detection device according to claim 1, characterized in that, The detection pool slot (4) includes a first card wall (41), a second card wall (42), a third card wall (43) and a fourth card wall (44) connected in sequence; the inner wall of the first card wall (41) is provided with a second arc-shaped groove (411), and the inner wall of the third card wall (43) is provided with a third arc-shaped groove (431). The second arc-shaped groove (411) and the third arc-shaped groove (431) are symmetrically arranged on both sides of the detection pool (5).

5. The electromagnetic interference-resistant nanoporous single-molecule detection device according to claim 4, characterized in that, The first card wall (41) is provided with a threaded hole (45), and a fastener is provided on the threaded hole (45); when in use, the fastener abuts against the detection pool (5).

6. The electromagnetic interference-resistant nanoporous single-molecule detection device according to claim 1, characterized in that, The shielding box body (1) is provided with an electrode socket (3), and the electrode is connected to the pulse source through the electrode socket (3).

7. The electromagnetic interference-resistant nanoporous single-molecule detection device according to claim 1, characterized in that, The shielding box body (1) includes a first side wall (11), a second side wall (12), a third side wall (13) and a fourth side wall (14) connected in sequence; the connection between the first side wall (11), the second side wall (12) and the third side wall (13) is smoothly transitioned.

8. The electromagnetic interference-resistant nanoporous single-molecule detection device according to claim 7, characterized in that, The fourth sidewall (14) is provided with rounded corner components (15) at the connection between it and the first sidewall (11) and the third sidewall (13).

9. The electromagnetic interference-resistant nanoporous single-molecule detection device according to claim 1, characterized in that, The shielding box body (1) also includes a base plate (16), on which a number of fixing holes (17) are provided.

10. The electromagnetic interference-resistant nanoporous single-molecule detection device according to claim 1, characterized in that, The shielding box cover (2) includes a cover body (21) and a sealing side (22) connected to the cover body (21).

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