An ultra-sensitive protein detection device based on chromatography and amplified signal coupling

Abstract

The utility model belongs to detection device technical field especially relates to a kind of based on chromatography and amplification signal coupling's super-sensitive protein detection device, including enzyme label unit, the enzyme label unit is cylindrical, and it is used to combine enzyme with target protein, and detectable signal is generated using enzyme catalysis reaction;Fixing frame, the fixing frame is used to install enzyme label unit;Stirring component, the stirring component is used to stir fluid inside enzyme label unit, the application is rotated by using driving member to drive shaft, while stirring fluid by stirring blade driven by shaft rotation, eccentric wheel is rotated by shaft, when eccentric wheel and swing plate contact, swing plate will be pushed to swing by swing mouth, while swing plate drives plate body to swing, plate body completes turbulence to fluid by through opening, groove, protrusion, so that enzyme and target protein inside fluid contact more fully.

Description

Technical Field

[0001] This invention belongs to the field of detection device technology, and particularly relates to an ultrasensitive protein detection device based on the coupling of chromatography and amplified signals. Background Technology

[0002] The ultrasensitive protein detection device based on the coupling of chromatography and signal amplification combines chromatography technology and signal amplification mechanism to achieve high-sensitivity detection of low-concentration proteins.

[0003] For example, Chinese patent CN221765485U discloses a specific protein detection device that solves the technical problems of existing specific protein detection devices having a small detection range and low detection efficiency, making them unsuitable for rapid detection.

[0004] While the above technology enables the fluid to rotate along the circumference by starting the stirring motor and sequentially driving the stirring magnet and stirring element, the degree of mixing between the fluids perpendicular to the circumference is small, and the fluid exchange is insufficient. Utility Model Content

[0005] In view of this, the present invention provides an ultrasensitive protein detection device based on the coupling of chromatography and amplified signals, comprising:

[0006] An enzyme labeling unit, which is cylindrical in shape, is used to bind an enzyme to a target protein and generate a detectable signal by using an enzyme-catalyzed reaction.

[0007] A mounting bracket for mounting enzyme labeling units;

[0008] A stirring assembly for stirring the fluid inside the enzyme labeling unit;

[0009] A plurality of turbulence components are provided for turbulentizing the fluid inside the enzyme labeling unit to increase mixing uniformity.

[0010] Preferably, the stirring assembly includes:

[0011] A driving component, which is fixed to a fixed frame, is used to provide stirring power and turbulent power;

[0012] A rotating shaft is fixed to the output end of the drive component, and the rotating shaft is used to transmit the power provided by the drive component.

[0013] A plurality of stirring blades are fixed to the ends of the rotating shaft, and the plurality of stirring blades are disposed within the enzyme labeling unit;

[0014] An eccentric wheel is fixed to the rotating shaft and is located outside the enzyme labeling unit. The eccentric wheel is used to drive the movement of several turbulent components.

[0015] Preferably, the turbulence component includes:

[0016] A swing port, wherein the swing port is provided on the enzyme labeling unit;

[0017] A swing plate, which is rotatably connected to a swing opening via a rotating shaft, and the swing plate is in contact with an eccentric wheel;

[0018] The plate body is fixed to the swing plate and is disposed inside the enzyme labeling unit. The plate body is used to disrupt the fluid state inside the enzyme labeling unit.

[0019] Preferably, the interior of the plate has several openings.

[0020] Preferably, one side of the plate has a plurality of grooves.

[0021] Preferably, the other side of the plate has several protrusions.

[0022] Preferably, an enzyme tube and a protein tube are fixedly connected to the enzyme labeling unit, the enzyme tube being used for the input of the relevant enzyme, and the protein tube being used for the input of the target protein.

[0023] This application uses a drive component to drive a rotating shaft to rotate. The rotating shaft drives the stirring blades to stir the fluid. At the same time, the rotating shaft drives the eccentric wheel to rotate. When the eccentric wheel contacts the swing plate, it pushes the swing plate to swing through the swing port. At the same time, the swing plate drives the plate body to swing. The plate body completes the turbulence of the fluid through the port, groove and protrusion, so that the enzyme inside the fluid and the target protein can have more sufficient contact. Attached Figure Description

[0024] Figure 1 This is an axial view of the present invention;

[0025] Figure 2 This is a partial axial view of the present invention;

[0026] Figure 3 This is a cross-sectional view of the stirring assembly in this utility model;

[0027] Figure 4 This is a structural diagram of the turbulence component in this utility model;

[0028] Figure 5 This is a structural diagram of one side of the plate in this utility model;

[0029] Figure 6 This is a structural diagram of the other side of the plate in this utility model.

[0030] The markings in the diagram are as follows:

[0031] 100, Fixing frame; 200, Enzyme labeling unit; 300, Enzyme tube; 400, Protein tube; 500, Stirring assembly; 510, Drive component; 520, Rotating shaft; 530, Stirring blade; 540, Eccentric wheel; 600, Turbulence assembly; 610, Swing port; 620, Swing plate; 630, Plate body; 631, Through port; 632, Tank body; 633, Protrusion. Detailed Implementation

[0032] The technical solutions of the embodiments of this application will be clearly described below with reference to the accompanying drawings. Obviously, the described embodiments are only some, not all, of the embodiments of this application. All other embodiments obtained by those skilled in the art based on the embodiments of this application are within the scope of protection of this application.

[0033] like Figures 1-2 As shown, this embodiment provides an ultrasensitive protein detection device based on the coupling of chromatography and amplified signals, including: a fixture 100, an enzyme labeling unit 200, an enzyme tube 300, a protein tube 400, a stirring assembly 500, and several turbulence components 600.

[0034] The fixture 100 is a rectangular hollow frame, and the enzyme labeling unit 200 is installed in the hollow part inside the fixture 100. The enzyme labeling unit 200 is cylindrical and is used to bind the enzyme to the target protein obtained by chromatography. The enzyme-catalyzed reaction generates a detectable signal, thereby amplifying the signal of the target protein and facilitating subsequent ultrasensitive protein detection. An enzyme tube 300 and a protein tube 400 are fixedly connected to the enzyme labeling unit 200. The enzyme tube 300 is used to input the relevant labeling enzyme into the enzyme labeling unit 200, and the protein tube 400 is used to input the target protein separated by the chromatography unit into the enzyme labeling unit 200.

[0035] The stirring component 500 is used to stir the fluid inside the enzyme labeling unit 200 to improve the binding efficiency between the enzyme and the target protein. However, because the stirring component 500 drives the fluid to rotate, the mixing degree between the fluids perpendicular to the rotation direction is small, resulting in insufficient contact between the enzyme and the target protein. Several turbulence components 600 will turbulentize the fluid inside the enzyme labeling unit 200 to increase the mixing uniformity between the enzyme and the target protein.

[0036] like Figure 3 As shown, the stirring assembly 500 includes: a drive component 510, a rotating shaft 520, several stirring blades 530, and an eccentric wheel 540.

[0037] The drive component 510 is fixed to the fixed frame 100, preferably a motor, but manual rotation can also be used to save energy and assembly costs. It requires rotational force and provides stirring and turbulent flow power. The rotating shaft 520 is fixed to the output end of the drive component 510, passing sequentially through the fixed frame 100 and the enzyme labeling unit 200. The rotating shaft 520 transmits the power provided by the drive component 510. Several stirring blades 530 are fixed to the ends of the rotating shaft 520 and drive the fluid inside the enzyme labeling unit 200 to rotate, improving the binding efficiency between the enzyme and the target protein. The stirring blades 530 are disposed within the enzyme labeling unit 200. The eccentric wheel 540 has a circular shape, with its center not on the same axis as the rotating shaft 520. The eccentric wheel 540 is fixed to the rotating shaft 520 and is disposed outside the enzyme labeling unit 200. The eccentric wheel 540 drives the movement of several turbulent flow components 600.

[0038] like Figure 4 As shown, the turbulence component 600 includes: a swing port 610, a swing plate 620, and a plate body 630.

[0039] The swing port 610 is located on the enzyme labeling unit 200, providing a certain space for the swing plate 620 to swing. The swing plate 620 is rotatably connected to the swing port 610 via a rotating shaft (Z1). When the swing plate 620 contacts the eccentric wheel 540, the eccentric wheel 540 rotates around the rotating shaft 520, causing the swing plate 620 to swing around the rotating shaft (Z1) within the swing port 610. Torsion springs are installed inside the rotating shaft (Z1) and the swing plate 620, allowing the swing plate 620 to deflect towards the eccentric wheel 540. The plate body 630 is fixed to the swing plate 620 and is located within the enzyme labeling unit 200. When the swing plate 620 swings via the swing port 610, it synchronously drives the plate body 630 to swing within the enzyme labeling unit 200, thereby disrupting the fluid state inside the enzyme labeling unit 200 and ensuring more thorough contact between the enzyme and the target protein within the fluid. Plate 630 is configured to not interfere with the stirring assembly when it oscillates.

[0040] To further improve the turbulence effect of plate 630, such as Figures 5-6 As shown, the plate 630 has several openings 631 inside. The openings 631 are elongated channels that penetrate the plate 630. When the plate 630 is oscillating, some fluid will pass through the openings 631, while some fluid cannot pass through, thereby increasing the degree of turbulence of the fluid between the plate 630 and the openings 631.

[0041] To further improve the turbulence effect of the plate 630, in a more preferred embodiment, a plurality of grooves 632 are provided on one side of the plate 630. The grooves 632 are elongated groove structures. By setting the grooves 632, when the plate 630 swings, some fluid enters the interior of the grooves 632 to form local vortices, thereby increasing the degree of turbulence of the fluid between the plate 630 and the grooves 632.

[0042] In a further embodiment, a plurality of protrusions 633 are provided on the other side of the plate 630. By setting the protrusions 633, when the plate 630 swings, it is separated by the protrusions 633 and generates lateral flow, thereby increasing the degree of turbulence of the fluid between the plate 630 and the protrusions 633.

[0043] In this invention, the electrical components are controlled by an external controller that is paired with them. The control circuit can be implemented by a person skilled in the art through simple programming. It is common knowledge in the field and is used without modification. Furthermore, since this invention is mainly used to protect mechanical devices, the control method and circuit connection will not be explained in detail.

[0044] The embodiments of this application have been described above with reference to the accompanying drawings. Unless otherwise specified, the embodiments and features in the embodiments of this application can be combined with each other. This application is not limited to the specific embodiments described above. The specific embodiments described above are merely illustrative and not restrictive. Those skilled in the art can make many other forms under the guidance of this application without departing from the spirit and scope of the claims, and all of these forms are within the protection scope of this application.

Claims

1. A highly sensitive protein detection device based on the coupling of chromatography and amplified signals, characterized in that, include: An enzyme labeling unit (200) is cylindrical and is used to bind an enzyme to a target protein, thereby generating a detectable signal through an enzyme-catalyzed reaction. A mounting bracket (100) is used to mount the enzyme labeling unit (200); A turbulence assembly (600) is used to turbulentize the fluid inside the enzyme labeling unit (200) to increase mixing uniformity; A stirring assembly (500) is used to stir the fluid inside the enzyme labeling unit (200). The stirring assembly (500) includes a rotating shaft (520) and an eccentric wheel (540) connected to the rotating shaft (520). The rotating shaft (520) rotates to drive the eccentric wheel (540) to rotate, thereby driving the turbulence assembly (600) to move.

2. The ultrasensitive protein detection device based on the coupling of chromatography and amplified signals according to claim 1, characterized in that, The stirring assembly (500) includes: A drive unit (510) is fixed to a fixed frame (100), and the drive unit (510) is used to provide stirring power and turbulent power; A plurality of stirring blades (530) are fixed to the ends of the stirring blades (530) and the rotating shaft (520), and the plurality of stirring blades (530) are disposed within the enzyme labeling unit (200).

3. The ultrasensitive protein detection device based on the coupling of chromatography and amplified signals according to claim 1, characterized in that, The turbulence component (600) includes: A swing port (610) is provided on the enzyme labeling unit (200); A swing plate (620) is rotatably connected to a swing port (610) via a rotating shaft, and the swing plate (620) is in contact with an eccentric wheel (540); The plate (630) is fixed to the swing plate (620). The plate (630) is disposed inside the enzyme labeling unit (200). The plate (630) is used to disrupt the fluid state inside the enzyme labeling unit (200).

4. The ultrasensitive protein detection device based on the coupling of chromatography and amplified signals according to claim 3, characterized in that, The plate (630) has several openings (631) inside.

5. The ultrasensitive protein detection device based on the coupling of chromatography and amplified signals according to claim 3, characterized in that, The plate (630) has several grooves (632) on one side.

6. The ultrasensitive protein detection device based on the coupling of chromatography and amplified signals according to claim 3, characterized in that, The other side of the plate (630) has several protrusions (633).

7. The ultrasensitive protein detection device based on the coupling of chromatography and amplified signals according to claim 1, characterized in that, Enzyme labeling unit (200) is fixedly connected to enzyme tube (300) and protein tube (400), respectively. Enzyme tube (300) is used for input of related enzymes, and protein tube (400) is used for input of target proteins.

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