Special tissue separation equipment for red-topped bamboo fungus mother culture

Abstract

This utility model provides a special tissue separation device for *Dictyophora indicum* mother culture, belonging to the field of edible fungus spawn cultivation technology. It includes a workbench with an alcohol lamp, tweezers, and a cutting knife. The cutting knife consists of an arc-shaped handle and an arc-shaped blade. The arc-shaped blade is fixedly mounted on the back of the handle and has a soft rubber pad. Multiple support plates are mounted on the handle, each equipped with an ultraviolet lamp. Using this special tissue separation device for *Dictyophora indicum* mother culture, the ultraviolet lamp can disinfect the cut surface of the bamboo fungus egg during cutting, and the soft rubber pad effectively limits the cutting depth, reducing unnecessary damage to the mycelial tissue and ensuring the purity and quality of the mother culture. After tissue separation, the bamboo fungus egg can still be covered with soil for fruiting, without affecting the later observation of its fruiting-related biological and agronomical traits, thus improving breeding efficiency.

Description

Technical Field

[0001] This utility model belongs to the field of edible fungi strain cultivation technology, and specifically relates to a special tissue separation device for the mother culture of red-topped bamboo fungus. Background Technology

[0002] Red-topped bamboo fungus is a rare edible fungus. The young fruiting bodies formed underground use bamboo fungus eggs as primordia. These eggs resemble spheres the size of chicken or duck eggs, with an outer layer and an inner layer of undifferentiated mycelial tissue. Tissue isolation from red-topped bamboo fungus is a crucial method for obtaining pure mother culture. Bamboo fungus eggs, as the fruiting body primordia, are tender, highly active, and have a low contamination rate, making them ideal for isolation. Typically, tissue isolation involves aseptically picking a small amount of mycelial tissue from inside the bamboo fungus egg, inoculating it onto a culture medium, and then culturing and purifying it to remove contaminating bacteria, thus obtaining a qualified mother culture.

[0003] In traditional tissue separation operations, when workers use a cutting knife to cut bamboo fungus eggs, it is not easy to accurately control the cutting depth. The cutting knife can easily damage the mycelial tissue inside the bamboo fungus eggs, resulting in damage to the mycelial tissue and affecting the purity and quality of the subsequent mother culture. Furthermore, if the cut of the bamboo fungus eggs is not disinfected in time, it can easily increase the risk of contamination.

[0004] The present invention aims to provide a special tissue separation device for *Dictyophora indicum* mother culture that is easy to operate and reduces the risk of mycelial tissue contamination, in order to solve the problems existing in the prior art. Utility Model Content

[0005] The technical problem to be solved by this utility model is to reduce the damage of the blade to the mycelial tissue inside the bamboo fungus egg, ensure the integrity of the mycelial tissue, and disinfect the cut of the bamboo fungus egg in a timely manner.

[0006] To solve the above-mentioned technical problems, the technical solution adopted by this utility model is: a special tissue separation device for red-topped bamboo fungus mother culture, including a workbench, on which an alcohol lamp, tweezers, and a cutting knife are placed. The cutting knife consists of an arc-shaped handle and an arc-shaped blade. The arc-shaped blade is fixedly installed on the back of the arc-shaped handle, that is, the alcohol lamp can disinfect the tweezers and the arc-shaped blade on the cutting knife, that is, the tweezers can be used to grasp the mycelial tissue inside the bamboo fungus egg. A soft rubber pad is provided on the arc-shaped blade, that is, the soft rubber pad can prevent the arc-shaped blade from cutting deep into the inside of the bamboo fungus egg. Multiple support plates are installed on the arc-shaped handle, and each support plate is provided with an ultraviolet irradiation lamp for disinfecting the arc-shaped blade and the cut of the bamboo fungus egg.

[0007] Optionally, a sub-control switch for controlling the UV irradiation lamp is installed on the support plate, and a master control switch is installed on the curved handle. The sub-control switch and master control switch facilitate the operation of the UV irradiation lamp by the staff. A handle is installed on the inner curved surface of the curved handle, and a protective cover is provided on the curved blade. The handle makes it easy for the user to pick up the curved handle and the curved blade.

[0008] Optionally, a clamping assembly for holding bamboo fungus eggs is installed on the worktable. The clamping assembly includes a placement block with a placement groove for placing half a bamboo fungus egg. A pull rod is slidably installed on the placement block, passing through one side of the placement groove. An arc-shaped pressing plate that fits against the inner wall of the placement groove is installed on the pull rod. The pull rod allows the user to push the arc-shaped pressing plate, which can clamp and fix bamboo fungus eggs of different sizes in the placement groove.

[0009] Optionally, a stop block is installed on the pull rod, and a compression spring is connected between the stop block and the placement block, which is wrapped around the pull rod. The elastic support of the compression spring can make the arc-shaped pressing plate fit against the inner wall of the placement slot.

[0010] Optionally, a cover plate is installed on the workbench. The cover plate consists of a back plate, a top plate, and two side plates. The top plate is equipped with a UV lamp corresponding to the workbench. That is, the UV lamp can disinfect the workbench in time during the mycelial tissue extraction process.

[0011] Optionally, a vent is provided on the rear panel to facilitate the discharge of gases produced when the alcohol lamp is burning, thus preventing the gases from accumulating near the workbench.

[0012] Compared with the prior art, the beneficial effects of this utility model are as follows:

[0013] 1. This utility model, by setting a soft rubber pad on the curved blade, can prevent the curved blade from cutting deep into the interior of the bamboo fungus egg. Compared with traditional technology, this can reduce the damage of the curved blade to the mycelial tissue inside the bamboo fungus egg and ensure the integrity of the mycelial tissue.

[0014] 2. By setting up an ultraviolet irradiation lamp, this utility model can disinfect the cut surfaces of the curved blade and bamboo fungus eggs in a timely manner, thereby reducing the risk of contamination of the mycelial tissue.

[0015] 3. The present invention, through the clamping component, can conveniently clamp and fix bamboo fungus eggs of different sizes in the placement groove, reducing human hand contact with bamboo fungus eggs, thereby further reducing the probability of contamination of mycelial tissue during the extraction process. At the same time, fixing the bamboo fungus eggs can improve the efficiency of tissue separation.

[0016] 4. By limiting the cutting depth of the curved blade, this utility model can reduce damage to bamboo fungus eggs while ensuring normal tissue separation. This allows for normal soil covering and mushroom growth in the later stages, facilitating the observation of its mushroom-related biological and agronomic traits and improving breeding efficiency. Attached Figure Description

[0017] The present invention will now be described in further detail with reference to the accompanying drawings and specific embodiments.

[0018] Figure 1 This is a three-dimensional structural schematic diagram of the present invention;

[0019] Figure 2 This is a three-dimensional structural diagram of the cutting blade and protective sleeve of this utility model;

[0020] Figure 3 This is a three-dimensional structural diagram of the soft rubber gasket of this utility model;

[0021] Figure 4 This is a three-dimensional structural diagram of the clamping component of this utility model;

[0022] Figure 5 This is a bottom view of the structure of this utility model.

[0023] Explanation of reference numerals in the attached diagram: 1. Workbench; 11. Alcohol lamp; 12. Tweezers; 13. Cover plate; 131. Back plate; 132. Top plate; 133. Side plate; 14. Second ultraviolet irradiation lamp; 15. Vent hole; 2. Cutting assembly; 21. Cutting blade; 211. Curved handle; 212. Curved blade; 22. Soft rubber pad; 23. Support plate; 24. First ultraviolet irradiation lamp; 25. Sub-control switch; 26. Main control switch; 27. Handle; 28. Protective cover; 3. Clamping assembly; 31. Placement block; 32. Placement slot; 33. Pull rod; 34. Curved pressing plate; 35. Stop block; 36. Compression spring. Detailed Implementation

[0024] To better understand this utility model, the following embodiments further illustrate its content, but the scope of protection of this utility model is not limited to the embodiments described below. Numerous specific details are set forth in the following description to provide a more thorough understanding of this utility model. However, it will be apparent to those skilled in the art that this utility model can be practiced without one or more of these details.

[0025] like Figure 1-5As shown, a special tissue separation device for *Dictyophora indicum* mother culture includes a workbench 1, a cutting component 2, and a clamping component 3. The cutting component 2 is placed on the workbench 1 and is used to cut the *Dictyophora indicum* eggs. During the cutting process, the cut surface of the *Dictyophora indicum* eggs can be disinfected in time, and damage to the mycelial tissue inside the *Dictyophora indicum* eggs can be reduced. The clamping component 3 is fixedly installed on the workbench 1 and is used to clamp the *Dictyophora indicum* eggs, reducing the time that the *Dictyophora indicum* eggs are held by hand, which can reduce the probability of hand contact with mycelial tissue and reduce the risk of contamination.

[0026] like Figure 2 and Figure 3 As shown, the cutting assembly 2 includes a cutting blade 21, an arc-shaped handle 211, an arc-shaped blade 212, a soft rubber pad 22, a support plate 23, an ultraviolet irradiation lamp 24, a sub-control switch 25, a main control switch 26, a handle 27, and a protective cover 28. The cutting blade 21 consists of an arc-shaped handle 211 and an arc-shaped blade 212. The arc-shaped blade 212 is fixedly installed on the back of the arc-shaped handle 211. Soft rubber pads 22 are provided on the arc-shaped blade 212. There are two soft rubber pads 22, located on both sides of the arc-shaped blade 212. When the arc-shaped blade 212 cuts into the inside of the bamboo fungus egg, the soft rubber pads 22 can limit the depth of the blade's cut and prevent damage to the mycelial tissue. Multiple support plates 23 are installed on the arc-shaped handle 211. Ultraviolet irradiation lamps 24 are installed on the support plates 23. The ultraviolet irradiation lamps 24 shine directly on the arc-shaped blade 212, which can disinfect the arc-shaped blade 212 and the cut surface of the bamboo fungus egg in time.

[0027] A sub-control switch 25 for controlling the ultraviolet irradiation lamp 24 is installed on the support plate 23, and a main control switch 26 is installed on the curved handle 211. The sub-control switch 25 and the main control switch 26 facilitate the operation of the ultraviolet irradiation lamp 24 by the operator, which can open it intermittently or all at once, depending on the actual situation. A handle 27 is installed on the inner curved surface of the curved handle 211, and a protective cover 28 is provided on the curved blade 212. The handle 27 makes it easy for the operator to pick up the curved handle 211 and the curved blade 212. The protective cover 28 can be put on the curved blade 212 when the cutting blade 21 is not in use to prevent the curved blade 212 from causing injury to the operator.

[0028] like Figure 4As shown, the clamping assembly 3 includes a placement block 31, a placement groove 32, a through groove, a pull rod 33, an arc-shaped pressing plate 34, a stop block 35, and a compression spring 36. The placement block 31 is fixedly installed on the workbench 1. The placement block 31 has a placement groove 32 for placing half a bamboo fungus egg. The interior of the placement groove 32 is set in a semi-circular groove so that it can fit the outer surface of the bamboo fungus egg. The placement block 31 has a through groove. A pull rod 33 is slidably installed on the placement block 31, passing through one side of the placement groove 32. The pull rod 33 passes through the through groove. An arc-shaped pressing plate 34 that fits against the inner wall of the placement groove 32 is installed on the pull rod 33. The pull rod 33 makes it easy for the user to push the arc-shaped pressing plate 34. The arc-shaped pressing plate 34 can clamp and fix the bamboo fungus egg in the placement groove 32, which can accommodate bamboo fungus eggs of different sizes and enhance the practicality of the device.

[0029] like Figure 1 and Figure 5 As shown, an alcohol lamp 11 and tweezers 12 are placed on the workbench 1. A cover plate 13 and an ultraviolet lamp 14 are also installed on the workbench 1. The cover plate 13 consists of a back plate 131, a top plate 132, and two side plates 133. The alcohol lamp 11 can sterilize the tweezers 12 and the curved blade 212 on the cutting knife 21. The tweezers 12 are used to pick up the mycelial tissue inside the bamboo fungus egg and then place the individual mycelial tissues in the culture medium. The cover plate 13 can shield the workbench 1. The top plate 132 is equipped with a corresponding ultraviolet irradiation lamp 14 for the workbench 1. The ultraviolet irradiation lamp 14 can disinfect the workbench 1 in time during the mycelial tissue extraction process. The back plate 131 of the cover plate 13 is provided with ventilation holes 15. The design of the ventilation holes 15 is to ensure that the gas produced when the alcohol lamp 11 is burning can be discharged smoothly, preventing the gas from accumulating near the workbench 1 and affecting the breathing comfort of the staff. At the same time, it can reduce the potential damage of the gas to the sterile environment on the workbench 1.

[0030] Working principle: When using, first thoroughly disinfect the workbench 1, tweezers 12, cutting knife 21 and the outer surface of the bamboo fungus egg. Then place the alcohol lamp 11, tweezers 12 and cutting knife 21 in a suitable position on the workbench 1. Place the bamboo fungus egg that needs to be separated into the placement slot 32 of the clamping component 3. Turn on the ultraviolet irradiation lamp 14 to irradiate the workbench 1 with ultraviolet light.

[0031] Afterwards, the staff picked up the cutting blade 21 with handle 27, turned on the ultraviolet lamp 24, and the light from the ultraviolet lamp 24 shone on the cut of the curved blade 212, causing the curved blade 212 to cut the bamboo fungus egg. The staff also manually rotated the bamboo fungus egg inside the placement groove 32. During the cutting process, the ultraviolet lamp 24 could disinfect the bamboo fungus egg at the cut in time, and the soft rubber pad 22 could effectively limit the cutting depth of the blade and reduce unnecessary damage to the mycelial tissue inside the bamboo fungus egg. After the bamboo fungus egg was cut around one circle, it was separated into two halves. One half was placed in the placement groove 32, and the stop block 35 and the pull rod 33 were pressed to make the curved pressing plate 34 and the placement groove 32 clamp and fix the half of the bamboo fungus egg. Finally, the staff used tweezers 12 to pick up the mycelial tissue inside the bamboo fungus egg and place it in the culture medium. The entire operation process is simple and efficient, and greatly reduces the risk of mycelial contamination, while improving the purity and quality of the mother culture.

[0032] Furthermore, it should be noted that, in the description of this utility model, unless otherwise explicitly specified and limited, the terms "installation," "connection," and "joining" should be interpreted broadly. For example, they can refer to a fixed connection, a detachable connection, or an integral connection; they can refer to a mechanical connection or an electrical connection; they can refer to a direct connection or an indirect connection through an intermediate medium; and they can refer to the internal connection of two components. Those skilled in the art can understand the specific meaning of the above terms in this utility model according to the specific circumstances.

[0033] The above description is the preferred embodiment of this utility model. It should be noted that for those skilled in the art, several improvements and modifications can be made without departing from the principle of this utility model, and these improvements and modifications should also be considered within the protection scope of this utility model.

Claims

1. A special tissue separation device for *Dictyophora indicum* mother culture, characterized in that: The device includes a workbench (1), on which an alcohol lamp (11), tweezers (12) and a cutting knife (21) are placed. The cutting knife (21) consists of an arc-shaped handle (211) and an arc-shaped blade (212). The arc-shaped blade (212) is fixedly installed on the back of the arc-shaped handle (211). A soft rubber pad (22) is provided on the arc-shaped blade (212). Multiple support plates (23) are installed on the arc-shaped handle (211). Each support plate (23) is equipped with an ultraviolet irradiation lamp (24) for disinfecting the arc-shaped blade (212) and the cut of the bamboo fungus egg.

2. The apparatus for separating the mother-specialized tissue of Dictyophora rubrovolvata according to claim 1, wherein the apparatus is characterized by: The support plate (23) is equipped with a sub-control switch (25) for controlling the ultraviolet irradiation lamp (24), the arc-shaped handle (211) is equipped with a main control switch (26), the inner arc surface of the arc-shaped handle (211) is equipped with a handle (27), and the arc-shaped blade (212) is equipped with a protective cover (28).

3. The apparatus for separating the mother-specialized tissue of Dictyophora rubrovolvata according to claim 1, wherein the apparatus is characterized by: The workbench (1) is equipped with a clamping assembly (3) for clamping bamboo fungus eggs. The clamping assembly (3) includes a placement block (31). The placement block (31) has a placement groove (32) for placing half a bamboo fungus egg. A pull rod (33) that passes through one side of the placement groove (32) is slidably installed on the placement block (31). An arc-shaped pressing plate (34) that fits the inner wall of the placement groove (32) is installed on the pull rod (33).

4. The apparatus for separating the special tissue of the Dictyophora indusiata female seed according to claim 3, wherein the apparatus is characterized in that: A stop (35) is installed on the pull rod (33), and a compression spring (36) surrounding the pull rod (33) is connected between the stop (35) and the placement block (31).

5. The tissue separation device for *Dictyophora indicum* mother culture as described in claim 1, characterized in that: The workbench (1) is equipped with a cover plate (13), which consists of a rear plate (131), a top plate (132) and two side plates (133). The top plate (132) is equipped with an ultraviolet irradiation lamp (14) that irradiates the workbench (1).

6. The tissue separation device for *Dictyophora indicum* mother culture as described in claim 5, characterized in that: The rear plate (131) has ventilation holes (15).

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