A mouse bile duct-duodenal cannulation method
By designing a combined bile duct-duodenal cannulation for mice, the problem of significant harm to animals during bile collection in existing technologies has been solved. This approach enables long-term, periodic bile collection and ensures the survival of experimental animals, meeting animal welfare requirements.
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- SKILLSMODEL BIOTECH BEIJING
- Filing Date
- 2025-02-28
- Publication Date
- 2026-07-17
AI Technical Summary
Existing methods for collecting mouse bile are harmful to laboratory animals, make it difficult to collect bile over long periods, and do not meet animal welfare requirements.
A combined bile duct-duodenal cannula for mice was designed, comprising a duodenal cannula and a bile duct connecting tube. Made of silicone, it features multiple through holes and smooth rounded corners, combined with a three-way structure and a soft plug to construct an extracorporeal bile channel, reducing harm to the animal. Bile collection and injection are achieved through a stainless steel connecting tube.
This method enables long-term, periodic collection of mouse bile, reducing harm to laboratory animals, meeting animal welfare requirements, and ensuring the survival of laboratory animals.
Smart Images
Figure CN224505655U_ABST
Abstract
Description
Technical Field
[0001] This utility model relates to the field of surgical instruments for laboratory animals, and in particular to a combined bile duct-duodenal cannulation for mice. Background Technology
[0002] In drug development, it is often necessary to periodically collect bile from laboratory animals to determine drug half-life for efficacy assessment and toxicological analysis. Currently, the most common method for collecting bile from laboratory animals is to anesthetize them and extract it during surgery. This method is highly invasive, as the animals cannot survive for extended periods, making long-term periodic collection difficult and failing to meet animal welfare requirements. If long-term periodic bile collection needs to be performed outside the laboratory animal, and if bile collection is not required, it is necessary to connect the common bile duct and duodenum to create a bile flow pathway, then bile duct cannulation is an essential procedure.
[0003] Chinese Patent Publication No. CN219439522U discloses a rat duodenal cannula, comprising a first, second, third, and fourth segment of a catheter with the same inner and outer diameters, secured by a fixing knot. The first segment and the second segment are connected by two fixing knots, and the second, third, and fourth segments are each connected by a fixing knot. The length of the first segment is 0.6 to 1.0 cm, the second segment is 2.0 to 4.0 cm, the third segment is 7.0 to 9.0 cm, and the fourth segment is 8.0 to 10.0 cm. The first segment has multiple holes. Therefore, the above-mentioned rat duodenal cannula has the following problems: its outer diameter is large, limiting its application to rats; the catheter tip only leads to the outside, allowing only drug injection or enteric fluid detection in the duodenal segment, but not periodic bile collection; and the material of the tubing segments is unsuitable, as insertion into the duodenal segment may harm the experimental animal due to intestinal peristalsis. Utility Model Content
[0004] Therefore, this invention provides a combined bile duct-duodenal cannulation for mice to overcome the problem that existing technologies can only be used on rats, cannot perform periodic bile collection, and cause significant harm to animals.
[0005] To achieve the above objectives, this utility model provides a mouse bile duct-duodenal combined cannulation, comprising a duodenal cannula and a bile duct connecting tube connected in sequence, and further comprising an inner cannula fixing knot installed on the duodenal cannula and an outer cannula fixing knot and several catheter fixing knots installed on the bile duct connecting tube.
[0006] The internal cannula fixing knot is installed on the side close to where the duodenal cannula connects to the bile duct connecting tube, and the external cannula fixing knot is installed on the side close to where the bile duct connecting tube connects to the duodenal cannula. Each of the catheter fixing knots is installed sequentially after the external cannula fixing knot and gradually moves away from the duodenal cannula.
[0007] Furthermore, the middle section of the bile duct connecting tube is also provided with a three-way structure, and another branch of the three-way structure is located on the side of the bile duct connecting tube.
[0008] Furthermore, the three-way structure includes an experimental branch located on the side of the bile duct connecting tube and a soft plug that cooperates with the port of the experimental branch to close the port of the experimental branch.
[0009] The experimental branch is configured as a tapered structure with a diameter that gradually increases from the tee connection to the port of the experimental branch, and the soft plug is interference-fitted to the port of the experimental branch to close the port.
[0010] Furthermore, the duodenal cannula is made of silicone and is inserted into the inner side of the bile duct connecting tube with an interference fit. The insertion length between the duodenal cannula and the bile duct connecting tube is 6-7 mm.
[0011] Furthermore, the duodenal cannula has multiple equally spaced through holes on its sidewall to facilitate the flow of bile into the duodenum;
[0012] The duodenal cannula has smooth rounded corners on both sides of its end face.
[0013] Furthermore, the internal cannula fixing joint is made of silicone material, which is fitted onto the outer surface of the duodenal cannula with an interference fit and reinforced with adhesive.
[0014] Furthermore, the external insertion tube fixing joint is made of silicone material and is fitted onto the outer surface of the bile duct connecting tube with an interference fit, and is reinforced with adhesive.
[0015] Furthermore, the catheter fixation knot includes a first catheter fixation knot, a second catheter fixation knot, a third catheter fixation knot, a fourth catheter fixation knot, and a fifth catheter fixation knot; the catheter fixation knots are sequentially installed on the bile duct connecting tube, the first catheter fixation knot is installed on the side close to the external insertion tube fixation knot, the second catheter fixation knot and the third catheter fixation knot are sequentially installed away from the first catheter fixation knot in the middle section of the bile duct connecting tube, the fourth catheter fixation knot and the fifth catheter fixation knot are sequentially installed after the third catheter fixation knot, and the fifth catheter fixation knot is located on the side of the bile duct connecting tube near the port.
[0016] Furthermore, the catheter fixation knot is made of silicone material and is fitted onto the outer surface of the bile duct connecting tube with an interference fit, and is reinforced with adhesive.
[0017] Furthermore, it also includes a stainless steel connecting tube, which is used to disconnect the connection on both sides of the bile duct connecting tube after the portion between the second catheter fixing knot and the third catheter fixing knot of the bile duct connecting tube is cut off; the stainless steel connecting tube is a hollow structure and its size matches that of the bile duct connecting tube.
[0018] Compared with the prior art, the beneficial effect of this utility model is that it constructs an in vitro bile channel for mice by sequentially connecting a duodenal cannula and a bile duct connecting tube, thus eliminating the harm to mice caused by multiple surgeries during periodic bile collection experiments.
[0019] Furthermore, by rationally distributing the position of the catheter fixation knot, this invention ensures that the overall device conforms to the physiological structure of the mouse when fixed, thereby reducing harm to the mouse and meeting the requirements for long-term wear.
[0020] Furthermore, this invention improves upon the use of silicone material for the duodenal cannula, with a smooth, rounded end, to prevent potential injury to mice during intestinal peristalsis.
[0021] Furthermore, by adding an experimental branch and a soft plug, this invention allows for convenient removal of the soft plug during the experiment to collect bile and inject bile salts into the duodenum. After the experiment, the soft plug is reinserted to prevent the mouse's internal body from communicating with the outside world, thus ensuring the mouse's survival.
[0022] Furthermore, this invention adds a stainless steel connecting tube. During the experiment, the bile duct connecting tube between the second and third catheter fixing points is cut to collect bile, and bile salts are injected into the duodenum. After the experiment, the stainless steel connecting tube is used to connect the bile ducts, allowing bile to flow into the duodenum and ensuring the survival of the mice. Attached Figure Description
[0023] Figure 1 This is a schematic diagram of the overall structure of this utility model;
[0024] Figure 2 This is a cross-sectional view of part A of the overall structure of this utility model;
[0025] Figure 3 This is a schematic diagram of the experimental branch of an embodiment of the present utility model;
[0026] Figure 4 This is a schematic diagram of the stainless steel connecting pipe according to an embodiment of the present utility model;
[0027] In the diagram: 1-duodenal cannula; 2-biliary duct connecting tube; 3-internal cannula fixation knot; 4-external cannula fixation knot; 51-first catheter fixation knot; 52-second catheter fixation knot; 53-third catheter fixation knot; 54-fourth catheter fixation knot; 55-fifth catheter fixation knot; 6-experimental branch; 7-soft plug; 8-stainless steel connecting tube; Detailed Implementation
[0028] To make the objectives and advantages of this utility model clearer, the utility model will be further described below with reference to the embodiments; it should be understood that the specific embodiments described herein are only for explaining this utility model and are not intended to limit this utility model.
[0029] Preferred embodiments of the present invention will now be described with reference to the accompanying drawings. Those skilled in the art should understand that these embodiments are merely illustrative of the technical principles of the present invention and are not intended to limit the scope of protection of the present invention.
[0030] It should be noted that in the description of this utility model, the terms "upper", "lower", "left", "right", "inner", "outer", etc., indicating the direction or positional relationship are based on the direction or positional relationship shown in the drawings. This is only for the convenience of description and does not indicate or imply that the device or element must have a specific orientation, or be constructed and operated in a specific orientation. Therefore, it should not be construed as a limitation of this utility model.
[0031] Furthermore, it should be noted that, in the description of this utility model, unless otherwise explicitly specified and limited, the terms "installation," "connection," and "joining" should be interpreted broadly. For example, they can refer to a fixed connection, a detachable connection, or an integral connection; they can refer to a mechanical connection or an electrical connection; they can refer to a direct connection or an indirect connection through an intermediate medium; and they can refer to the internal connection of two components. Those skilled in the art can understand the specific meaning of the above terms in this utility model according to the specific circumstances.
[0032] Specific embodiments are given below with reference to the accompanying drawings. These specific embodiments are only used to describe the technical solution of this utility model in detail and are not intended to limit the scope of protection of this application.
[0033] This utility model embodiment provides a combined bile duct-duodenal cannulation for mice. See [link to relevant documentation]. Figure 1 and Figure 2As shown, these are schematic diagrams of the overall structure of this utility model and cross-sectional views of part A of the overall structure of this utility model, respectively. It includes a duodenal cannula 1 and a bile duct connecting tube 2 connected in sequence, and also includes an internal cannula fixing knot 3 installed on the duodenal cannula 1 and an external cannula fixing knot 4 installed on the bile duct connecting tube 2, a first catheter fixing knot 51, a second catheter fixing knot 52, a third catheter fixing knot 53, a fourth catheter fixing knot 54 and a fifth catheter fixing knot 55.
[0034] Specifically, the duodenal cannula 1 is made of silicone and is inserted into the bile duct connecting tube 2 with an interference fit 6-7 mm inside. The side wall has multiple equally spaced through holes to allow mouse bile or artificial bile salts to flow into the mouse duodenum. The silicone material prevents possible injury to the mouse from the duodenal cannula 1 during intestinal peristalsis. The outer edges of its two end faces are smooth rounded corners. The smooth rounded corners on the through hole side can reduce friction with the inner wall of the duodenum, and the smooth rounded corners on the insertion side make it easy to insert the duodenal cannula 1 into the bile duct connecting tube 2 with an interference fit.
[0035] The bile duct connecting tube 2, made of polyurethane, is inserted 6-7 mm outside the duodenal cannula 1 with an interference fit to connect the mouse duodenum and bile duct. The polyurethane material is not easy to damage blood vessels, has good adhesion to the blood vessel wall, and is not easy to leak blood.
[0036] The first catheter fixing joint 51, the second catheter fixing joint 52, the third catheter fixing joint 53, the fourth catheter fixing joint 54, and the fifth catheter fixing joint 55 are made of silicone and are sequentially fitted onto the outer surface of the bile duct connecting tube 2 with an interference fit, and are reinforced with adhesive.
[0037] The internal cannula fixing joint 3 is made of silicone and is fitted with an interference fit to the outer surface of the insertion side of the duodenal cannula 1, and is reinforced with adhesive.
[0038] The external insertion tube fixing joint 4 is made of silicone material and is fitted with an interference fit on the outer surface of the insertion side of the bile duct connecting tube 2, and is reinforced with adhesive.
[0039] It is understood that the adhesive involved in this embodiment can be any of the existing technologies, and the reinforcement method is not limited here. Those skilled in the art can make appropriate selections according to the usage requirements, and all of them fall within the protection scope of this utility model, which will not be elaborated here.
[0040] Specifically, the following steps are involved in installing this invention on laboratory mice:
[0041] Duodenal cannula 1 and internal cannula fixation knot 3 are inserted into the mouse duodenum, while external cannula fixation knot 4 remains outside the duodenum. The two fixation knots are sutured with a purse-string suture, which can effectively prevent the leakage of intestinal contents and the resulting peritoneal inflammation. Since the cannula fixation knot 3 and external cannula fixation knot 4 are respectively installed on duodenal cannula 1 and bile duct connecting tube 2, suturing the two can effectively prevent bile duct connecting tube 2 from falling off duodenal cannula 1.
[0042] Extend the bile duct connecting tube 2 to the abdominal muscle of the mouse, and suture the first catheter fixing knot 51 to the abdominal muscle of the mouse.
[0043] Extend the bile duct connecting tube 2 from the subcutaneous tissue of the mouse's neck to the mouse's back and out of the mouse's back skin, and suture the second catheter fixing knot 52 to the mouse's back skin to fix it.
[0044] Insert the bile duct connecting tube 2 into the skin of the mouse's back at an appropriate distance from the suture site of the second catheter fixing knot 52, and suture the third catheter fixing knot 53 to the skin of the mouse's back.
[0045] Extend the bile duct connecting tube 2 from the back of the mouse to the abdominal muscle of the mouse, and suture the fourth duct fixation knot 54 to the abdominal muscle of the mouse.
[0046] Insert the far end of the bile duct connecting tube 2 into the mouse bile duct, and suture the fifth catheter fixation knot 55 to the mouse bile duct.
[0047] Specifically, for the duodenal cannula 1, preferably, its length is 13mm, its outer diameter is 0.635mm, and its through holes are 0.35mm in diameter, spaced 1.2mm apart, for a total of 3 holes.
[0048] Specifically, for the bile duct connecting tube 2, preferably, its length is 156 mm and its outer diameter is 0.635 mm.
[0049] Specifically, for the internal cannula fixation joint 3, preferably, its length is 1 mm and its outer diameter is 0.94 mm, and it is fixedly installed 5 mm away from the end face of the through hole of the duodenal cannula 1.
[0050] Specifically, for the external insertion tube fixing joint 4, preferably, its length is 2mm and its outer diameter is 0.94mm, and it is fixedly installed 3mm away from the insertion end face of the bile duct connecting tube 2.
[0051] Specifically, for the first catheter fixing knot 51, the second catheter fixing knot 52, the third catheter fixing knot 53, the fourth catheter fixing knot 54, and the fifth catheter fixing knot 55, preferably, their length is 2 mm and their outer diameter is 0.94 mm; the first catheter fixing knot 51 is fixedly installed 16 mm from the insertion end face of the bile duct connecting tube 2, the second catheter fixing knot 52 is fixedly installed 46 mm from the first catheter fixing knot 51, the third catheter fixing knot 53 is fixedly installed 25 mm from the second catheter fixing knot 52, the fourth catheter fixing knot 54 is fixedly installed 42 mm from the third catheter fixing knot 53, and the fifth catheter fixing knot 55 is fixedly installed 16 mm from the fourth catheter fixing knot 54.
[0052] See Figure 3 As shown, it is a schematic diagram of the experimental branch of this utility model embodiment, including experimental branch 6 and soft plug 7. Experimental branch 6 is set between the second conduit fixing joint 52 and the third conduit fixing joint 53. The diameter of the pipe gradually increases from the tee connection to the port of the experimental branch. The soft plug 7 is interference-fitted to the port of the experimental branch 6 to close the port.
[0053] Specifically, during the experiment, the soft plug was removed to collect bile, and bile salts were injected into the duodenum. After the experiment, the soft plug was reinserted to disconnect the mouse's internal body from the external environment, thus ensuring the mouse's survival.
[0054] See Figure 4 As shown, it is a schematic diagram of the stainless steel connecting pipe of this utility model embodiment, including a stainless steel connecting pipe 8, which is disposed between the second conduit fixing joint 52 and the third conduit fixing joint 53. It is a hollow structure. The stainless steel connecting pipe 8 and the bile duct connecting pipe 2 are installed by interference fit to disconnect the connection on both sides of the bile duct connecting pipe 2.
[0055] Specifically, during the experiment, the bile duct connecting tube 2 between the second duct fixation knot 52 and the third duct fixation knot 53 was cut to collect bile, and bile salts were injected into the duodenum. After the experiment, the bile was connected by a stainless steel connecting tube 8 to allow bile to flow into the duodenum and ensure the survival of the mice.
[0056] It is understood that the usage provided in this embodiment is not intended to limit the present invention. Those skilled in the art can make adaptive use according to the needs of actual scenarios, and all such use falls within the protection scope of the present invention, which will not be elaborated here.
[0057] The technical solution of this utility model has been described above with reference to the preferred embodiments shown in the accompanying drawings. However, it will be readily understood by those skilled in the art that the protection scope of this utility model is obviously not limited to these specific embodiments. Without departing from the principle of this utility model, those skilled in the art can make equivalent changes or substitutions to the relevant technical features, and the technical solutions after these changes or substitutions will all fall within the protection scope of this utility model.
Claims
1. A mouse cholangio-duodenal combined cannula, characterized in that, It includes a duodenal cannula and a bile duct connecting tube connected in sequence, and also includes an internal cannula fixing knot installed on the duodenal cannula and an external cannula fixing knot and several catheter fixing knots installed on the bile duct connecting tube; The internal cannula fixing knot is installed on the side close to where the duodenal cannula connects to the bile duct connecting tube, and the external cannula fixing knot is installed on the side close to where the bile duct connecting tube connects to the duodenal cannula. Each of the catheter fixing knots is installed sequentially after the external cannula fixing knot and gradually moves away from the duodenal cannula.
2. The mouse bile duct-duodenal cannulation according to claim 1, characterized in that, The bile duct connecting tube is also provided with a three-way structure in the middle section, and another branch of the three-way structure is located on the side of the bile duct connecting tube.
3. The mouse cholangio-duodenal combined cannula according to claim 2, wherein, The three-way structure includes an experimental branch located on the side of the bile duct connecting tube and a soft plug that cooperates with the port of the experimental branch to close the port of the experimental branch. The experimental branch is configured as a tapered structure with a diameter that gradually increases from the tee connection to the port of the experimental branch, and the soft plug is interference-fitted to the port of the experimental branch to close the port.
4. The mouse cholangio-duodenal combined cannula according to claim 1, wherein, The duodenal cannula is made of silicone and is inserted into the inner side of the bile duct connecting tube with an interference fit. The insertion length between the duodenal cannula and the bile duct connecting tube is 6-7 mm.
5. The mouse cholangio-duodenal combined cannula according to claim 3, wherein, The duodenal cannula has multiple equally spaced through holes on its side wall to facilitate the flow of bile into the duodenum. The duodenal cannula has smooth rounded corners on both sides of its end face.
6. The mouse cholangio-duodenal combined cannula of claim 1, wherein, The internal cannula fixing joint is made of silicone material and is fitted onto the outer surface of the duodenal cannula with an interference fit, and is reinforced with adhesive.
7. The mouse cholangio-duodenal combined cannula of claim 1, wherein, The external insertion tube fixing joint is made of silicone material and is fitted onto the outer surface of the bile duct connecting tube with an interference fit, and is reinforced with adhesive.
8. The mouse cholangio-duodenal combined cannula of claim 1, wherein, The catheter fixation joint includes a first catheter fixation joint, a second catheter fixation joint, a third catheter fixation joint, a fourth catheter fixation joint, and a fifth catheter fixation joint; the catheter fixation joints are sequentially installed on the bile duct connecting tube, the first catheter fixation joint is installed on the side close to the external cannula fixation joint, the second and third catheter fixation joints are sequentially installed away from the first catheter fixation joint in the middle section of the bile duct connecting tube, the fourth and fifth catheter fixation joints are sequentially installed after the third catheter fixation joint, and the fifth catheter fixation joint is located on the side of the bile duct connecting tube closer to the port.
9. The mouse cholangio-duodenal combined cannula according to claim 1 or 8, wherein, The catheter fixation knot is made of silicone material and is fitted onto the outer surface of the bile duct connecting tube with an interference fit, and is reinforced with adhesive.
10. The mouse bile duct-duodenal cannulation according to claim 8, characterized in that, It also includes a stainless steel connecting tube, which is used to disconnect the connection on both sides of the bile duct connecting tube after the portion between the second catheter fixing knot and the third catheter fixing knot is cut off. The stainless steel connecting pipe has a hollow structure and its dimensions match those of the bile duct connecting pipe.