一种基因合成装置
By linking the injection pump and detection components with the controller, the entire process of gene synthesis is automated, which solves the problem of relying on manual operation for reagent volume and temperature monitoring, improves synthesis efficiency and accuracy, and reduces enzyme activity fluctuations.
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- ZHONGHE GENE TECHNOLOGY (CHANGZHOU) CO LTD
- Filing Date
- 2025-08-19
- Publication Date
- 2026-07-17
AI Technical Summary
In existing gene synthesis devices, the precise measurement of reagent volume and real-time monitoring of synthesis temperature rely on manual operation, resulting in high labor intensity for staff and making it difficult to achieve efficient automation.
The system employs a controller to link the injection pump, diversion assembly, and synthesis assembly, along with an ultrasonic level gauge and thermal imager, to achieve automatic detection and control of solution volume and temperature, ensuring accuracy and stability while reducing manual intervention.
The entire gene synthesis process has been automated, reducing the workload of staff, improving synthesis efficiency and accuracy, and reducing enzyme activity fluctuations and base mismatch rates.
Smart Images
Figure CN224507074U_ABST
Abstract
Claims
1. A gene synthesizer apparatus, characterized by: The device includes a housing, an injection pump (1), a connecting assembly (2), a solution storage box (3), a diversion assembly (4), a synthesis assembly (5), a waste liquid collection assembly (6), a moving assembly (7), a detection assembly (8), and a controller. The injection pump (1), connecting assembly (2), solution storage box (3), diversion assembly (4), synthesis assembly (5), waste liquid collection assembly (6), moving assembly (7), detection assembly (8), and controller are all connected to or inside the housing. The connecting component (2) connects the diversion component (4) to the solution storage box (3), and the injection pump (1) is connected to the connecting component (2) between the diversion component (4) and the solution storage box (3); The synthesis component (5) is disposed on one side of the diversion component (4), and the connecting component (2) also connects the synthesis component (5) to the waste liquid collection component (6); The moving component (7) is disposed on one side of the synthesizing component (5), and the detection component (8) is connected to the moving component (7). The detection component (8) is located between the splitting component (4) and the synthesizing component (5). The detection component (8) includes a detection plate (81), an ultrasonic level gauge (82), and a thermal imager (83). The detection plate (81) is connected to the moving component (7). The ultrasonic level gauge (82) and the thermal imager (83) are both connected to the detection plate (81). The ultrasonic level gauge (82) is used to detect whether the amount of solution delivered to the synthesis component (5) corresponds to a preset value. The thermal imager (83) is used to detect whether the temperature of the solution on the synthesis component (5) corresponds to a preset value. The controller is connected to each component via leads and is used to control the operation of each component.
2. The apparatus of claim 1, wherein: The outer shell includes a base plate (9), and the moving component (7) includes a support block (71), a drive motor (73), a sliding block (76) and a rotating screw (77). The lower end of the support block (71) is connected to the base plate (9) and is located on one side of the composite component (5). The drive motor (73) is connected to the support block (71), the rotating screw (77) is connected to the support block (71), and the output shaft of the drive motor (73) is connected to one end of the rotating screw (77); The sliding block (76) is slidably connected to the support block (71), and the sliding block (76) is also threadedly connected to the rotating screw (77). The detection plate (81) is connected to the sliding block (76).
3. The device of claim 1, wherein: The connecting assembly (2) includes a first pipeline (203) and a two-way valve (201). One end of the first pipeline (203) extends into the solution storage box (3), and the two-way valve (201) is connected to the other end of the first pipeline (203). The two-way valve (201) is also connected to the diversion assembly (4). The syringe pump (1) is connected to the first pipeline (203). The syringe pump (1) is used to transport the solution in the solution storage box (3) to the diversion assembly (4) through the first pipeline (203) and the two-way valve (201).
4. The apparatus of claim 3, wherein: The connecting assembly (2) also includes a three-way valve (202) connected to the first pipeline (203), and the injection pump (1) is also connected to the three-way valve (202).
5. The apparatus of claim 3, wherein: The diversion assembly (4) includes a diversion bar (41) and an outlet pipe (42). The diversion bar (41) is connected to a rodless cylinder, and the outlet pipe (42) is vertically connected to the diversion bar (41). The two-way valve (201) is also connected to the outlet pipe (42). The outlet pipe (42) is used to discharge the solution drawn by the injection pump (1).
6. The apparatus of claim 5, wherein: The synthesis component (5) includes a constant temperature metal bath (51), which is connected inside the outer shell. A synthesis orifice plate (511) is connected to the constant temperature metal bath (51), and a liquid injection pipe (5111) is vertically opened on the synthesis orifice plate (5111). The liquid injection pipe (5111) is aligned with the liquid outlet pipe (42). The constant temperature metal bath (51) is connected to a rotary motor (52) and a rotary sealing plate (521). The rotary sealing plate (521) is connected to the output shaft of the rotary motor (52). The upper top wall of the rotary sealing plate (521) abuts against the lower opening of the injection pipe (5111). The rotary motor (52) drives the rotary sealing plate (521) to retain the solution in the injection pipe (5111). The constant temperature metal bath (51) has a waste liquid discharge chamber (53) inside, and the waste liquid discharge chamber (53) is located at the lower end of the constant temperature metal bath (51).
7. The apparatus of claim 6, wherein: The waste liquid collection assembly (6) includes a liquid pump (61) and a waste liquid collection box (62), both of which are connected inside the housing; The connecting assembly (2) includes a second pipe (204) and a third pipe (205). The liquid pump (61) is connected to the waste liquid discharge chamber (53) through the second pipe (204), and the liquid pump (61) is also connected to the waste liquid collection box (62) through the third pipe (205).
8. The apparatus of claim 1, wherein: The detection component (8) also includes a display screen, which is connected to the housing; The display screen is used to display data detected by the ultrasonic level gauge (82) and the thermal imager (83).