微孔芯片、样本转移装置和纳升移液系统
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- GUANGZHOU NAT LAB
- Filing Date
- 2025-05-22
- Publication Date
- 2026-07-17
AI Technical Summary
然而，基于微流控技术的微流控芯片难以实现高通量的生物反应(特别是阵列式的生物反应)，这严重影响了实验效率
[0021]相比已知的微孔板，微孔芯片具有明显更小的容积，从而能够减少生物反应所需的液体体积。另一方面，相比微流控芯片，第一微孔的容积又是足够大的，避免了采用微流控技术，以便生物反应能够在第一微孔中正常且稳定的进行。由于生物反应在多个第一微孔中独立的进行，微孔芯片的通量也得到了保证。换言之，微孔芯片兼顾了小体积和高通量。
Smart Images
Figure CN224507140U_ABST
Abstract
Claims
1. A micro-well chip, characterized by, include: ontology; as well as Multiple first micropores, which are blind pores and are formed on the upper surface of the body, have a depth of 500 μm to 2 mm and a diameter of 100 μm to 1500 μm, and the ratio of the depth of the first micropore to the diameter of the first micropore is 1:1 to 3:
1.
2. The micro-well chip of claim 1, wherein, The sidewall of the first micropore is parallel to the thickness direction of the body.
3. The micro-well chip of claim 1, wherein, The depth is greater than or equal to the diameter.
4. The micro-well chip of claim 1, wherein, The volume of the first micropore is 30 nmol to 3000 nmol.
5. The microwell chip of any one of claims 1 to 4, wherein, The number of the first micropores is greater than 384.
6. The micro-well chip of claim 5, wherein, The number of the first micropores is between 1,000 and 4,000.
7. The micro-well chip of claim 5, wherein, The distance between the geometric centers of two adjacent first micropores is 1 mm to 2.5 mm.
8. The microcavity chip of claim 7, wherein, Multiple of the first micropores are arranged in an array.
9. The micro-well chip of claim 7, wherein, The length of the body is 40mm to 110mm, and the width of the body is 25mm to 70mm.
10. The microcavity chip of claim 1, wherein, The microporous chip also includes: An elastic sealing film is disposed on the upper surface of the body. The elastic sealing film includes multiple through holes, and each of the multiple through holes corresponds to a multiple of the first micropores.
11. A sample transfer device, characterized by include: Microporous chip according to any one of claims 1 to 9; The transfer chip includes a plurality of second micropores, which are arranged in a mirror-symmetric manner with a plurality of first micropores, so that when the transfer chip and the micropore chip are arranged facing each other, the plurality of second micropores correspond one-to-one with the plurality of first micropores; as well as An elastic sealing membrane having multiple through holes, each of which corresponds one-to-one with a plurality of the first micropores. When the sample transfer device is in the sample transfer state, the elastic sealing film is clamped between the microporous chip and the transfer chip that are arranged facing each other, and the plurality of first micropores, the plurality of through holes and the plurality of second micropores are interconnected one by one.
12. The sample transfer device of claim 11, wherein, The diameter of the through hole is greater than or equal to the diameter of the first micropore.
13. The sample transfer device of claim 11, wherein, The elastic sealing film is fixedly connected to the upper surface of the microporous chip, and the plurality of through holes are interconnected with the plurality of first micropores in a one-to-one correspondence.
14. The sample transfer device of claim 13, wherein, The elastic sealing film is bonded to the upper surface of the microporous chip.
15. The sample transfer device according to any one of claims 11 to 14, wherein, The material of the elastic sealing membrane is selected from one or more of PDMS, silicone elastomers, liquid silicone rubber, ecoflex, and Dragon Skin® series silicones.
16. The sample transfer device according to any one of claims 11 to 14, wherein, The transfer chip may be the same as or different from the microporous chip.
17. A nanoliter pipetting system characterized in that, include: A chip base for fixing the microporous chip according to any one of claims 1 to 10, wherein when the microporous chip is fixed to the chip base, the first micropore faces upward; The nozzle has an outlet facing downwards and is used to generate picolinate-level droplets; as well as A movable component connected to the chip substrate or the nozzle, the movable component being used to move the chip substrate or the nozzle so that one of the plurality of first micro-holes is located below and aligned with the liquid outlet.
18. The nanoliter pipetting system of claim 17, wherein, The nanoliter pipetting system also includes: A liquid reservoir for storing liquids; A liquid conduit connecting the reservoir and the nozzle; A pressure control tube, one end of which is connected to the liquid reservoir, and the other end of which serves as a pressure control interface; A pressure control component is connected to the pressure control interface, and the pressure control component controls the pressure to allow liquid in the reservoir to enter and exit the liquid pipeline.
19. The nanoliter pipetting system of claim 18, wherein, The air pressure control component includes: air pump; A gas pipeline connecting the gas pump and the liquid reservoir; A pressure controller is connected to the gas pipeline to control the pressure inside the liquid reservoir.
20. The nanoliter pipetting system of claim 17, wherein, The nanoliter pipetting system also includes: An image acquisition component is disposed on one side of the liquid outlet to acquire a first image characterizing the relative positional relationship between the liquid outlet and the first micropore and / or a second image characterizing the droplet generation.
21. The nanoliter pipetting system of claim 17, wherein, The nozzle comprises a piezoelectric ceramic and a glass tube, and the nanoliter liquid transfer system further includes: An electric drive controller is connected to the piezoelectric ceramic and is used to output an electrical signal to the piezoelectric ceramic so that the piezoelectric ceramic acts on the glass tube.
22. The nanoliter pipetting system of claim 17, wherein, The nanoliter pipetting system also includes: A connector is provided with a mounting hole that penetrates the upper and lower surfaces of the connector, and the nozzle is inserted into the mounting hole and thus positioned longitudinally.
23. The nanoliter pipetting system of claim 22, wherein, The nozzle is detachably inserted into the mounting hole.
24. The nanoliter pipetting system of claim 23, wherein, The nozzle includes a first section and a second section, with the first section located below the second section. A stepped surface is formed at the connection between the first section and the second section, and the nozzle is supported on the upper surface of the connecting seat by means of the stepped surface.
25. The nanoliter pipetting system of claim 24, wherein, The diameter of the first segment is smaller than the diameter of the second segment.
26. The nanoliter pipetting system of claim 17, wherein, The nanoliter pipetting system also includes: An environmental control device includes an environmental control chamber, the microporous chip and the chip base, the nozzle and the moving component, all located within the environmental control chamber, wherein one or more of the temperature, humidity and air pressure of the environmental control chamber are adjustable.
27. The nanoliter pipetting system according to any one of claims 17 to 26, wherein, The chip base is provided with a mounting slot for placing the micro-hole chip, and a clamping component is provided in the mounting slot to clamp the micro-hole chip located in the mounting slot.
28. The nanoliter pipetting system of claim 27, wherein, The mounting groove has a slot on its side wall, and the clamping assembly is movably disposed within the slot.
29. The nanoliter pipetting system of claim 28, wherein, The clamping assembly includes a clamping block and an elastic member. The clamping block is configured to extend from the slot under the action of the elastic member in order to clamp the microporous chip located in the mounting slot.
30. The nanoliter pipetting system according to claim 29, wherein, The slot is provided with a first guide portion, and the clamping block is provided with a second guide portion. The first guide portion is connected to the second guide portion, and the first guide portion and the second guide portion can slide relative to each other.