核酸提取即时等温检测芯片
By integrating a nucleic acid extraction instant isothermal detection chip, the complexity of the separate operation of isothermal amplification detection is solved, which simplifies the process and improves applicability, making it easier to use fully automated nucleic acid detection equipment.
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- CAPITAL UNIV OF PHYSICAL EDUCATION & SPORTS
- Filing Date
- 2025-06-19
- Publication Date
- 2026-07-17
AI Technical Summary
Existing technologies that integrate target-specific nucleases for isothermal amplification detection require separate operations, which complicates the detection process and limits the applicability of point-of-care testing.
Design a nucleic acid extraction instant isothermal detection chip that integrates a lysis buffer chamber, a washing chamber, an elution buffer chamber, a LAMP reconstitution chamber, an Ago reconstitution chamber, an amplification tube, and a gDNA chamber, and is equipped with a pipette and a magnetic sleeve to achieve integrated detection without the need for separate tube operations.
It simplifies the testing process, improves the applicability and convenience of point-of-care testing, reduces the risk of aerosol contamination, is suitable for fully automated nucleic acid testing equipment, and reduces the difficulty and cost of operation.
Smart Images
Figure CN224513468U_ABST
Abstract
Claims
1. A nucleic acid extraction instant isothermal detection chip, characterized in that, The chip includes a chip body (1), on the first end face of which a nucleic acid extraction area and an isothermal amplification detection area are formed. The nucleic acid extraction area contains a lysis buffer chamber (111), a first washing chamber (112), a second washing chamber (113), and an elution buffer chamber (114). A magnetic bead is pre-placed in the second washing chamber (113). The isothermal amplification detection area contains a LAMP reconstitution chamber (121), an Ago reconstitution chamber (122), an amplification tube (123), and a gDNA chamber (124). The first end face is also provided with a first placement hole for placing a first pipette (131), a second placement hole for placing a second pipette (132), and a third placement hole for placing a magnetic sleeve (133). At least the openings of each of the lysis buffer chamber (111), the first washing chamber (112), the second washing chamber (113), the elution buffer chamber (114), the LAMP reconstitution chamber (121), the Ago reconstitution chamber (122), the amplification tube (123), and the gDNA chamber (124) are sealed with tin foil.
2. The nucleic acid extraction isothermal detection chip according to claim 1, characterized in that, A paraffin oil chamber (125) is also formed within the nucleic acid extraction area. 3.The nucleic acid extraction instant isothermal detection chip according to claim 2, characterized in that, The chip body (1) is disc-shaped, and the nucleic acid extraction area and the isothermal amplification detection area are respectively arc-shaped bands arranged around the center of the disc. 4.The nucleic acid extraction instant isothermal detection chip according to claim 3, characterized in that, The first pipette (131) and the magnetic sleeve (133) are located between the first end of the nucleic acid extraction area and the first end of the isothermal amplification detection area, and the second pipette (132) is located between the second end of the nucleic acid extraction area and the second end of the isothermal amplification detection area.
5. The nucleic acid extraction isothermal detection chip according to claim 4, characterized in that, Projected onto the first end face of the chip body (1), the central axes of the lysis buffer chamber (111), the first washing chamber (112), the second washing chamber (113), the elution buffer chamber (114), the LAMP reconstitution chamber (121), the Ago reconstitution chamber (122), the amplification tube (123), the gDNA chamber (124), the paraffin oil chamber (125), the first pipette (131), the second pipette (132), and the magnetic sleeve (133) are all located on a first circle, which is a circle concentric with the center of the chip body (1). 6.The nucleic acid extraction instant isothermal detection chip according to claim 1, characterized in that, The amplification tube (123) has multiple portions; and / or, the gDNA chamber (124) has multiple portions. 7.The nucleic acid extraction instant isothermal detection chip according to claim 6, characterized in that, The chip body (1) has multiple amplification tube placement holes, and each amplification tube (123) is mounted in each amplification tube placement hole. 8.The nucleic acid extraction instant isothermal detection chip according to claim 1, characterized in that, The amplification tube (123) is provided with an elastic sealing ring (1231) at its opening. The outer edge of the elastic sealing ring (1231) is connected to the inner wall of the opening of the amplification tube (123) as a whole. The diameter of the central hole of the elastic sealing ring (1231) is smaller than the diameter of the tip of the second pipette (132). 9.The nucleic acid extraction instant isothermal detection chip according to claim 1, characterized in that, The chip body (1) has a supporting ring wall (14) on the outer circumference of the second end face, and the second end face and the first end face are the two opposite end faces of the chip body (1). 10.The nucleic acid extraction instant isothermal detection chip according to claim 9, characterized in that, The support ring wall (14) has a protrusion (141) on the side edge away from the chip body (1).