一种农杆菌侵染植物愈伤装置
By designing an Agrobacterium infection device that combines connecting rods, threaded rods, and clamping blocks, the problem of damage caused by multiple transfers of callus tissue in traditional devices was solved, achieving a more efficient and uniform infection process and reducing the risk of damage and contamination.
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- WUHAN TIANWEN BIOTECHNOLOGY CO LTD
- Filing Date
- 2025-06-23
- Publication Date
- 2026-07-17
AI Technical Summary
Existing Agrobacterium-mediated plant callus infection devices require the repeated use of tools such as tweezers to transfer callus tissue during infection and subsequent treatment, resulting in physical damage, affecting cell structure and regeneration capacity, and reducing transformation efficiency.
A device for Agrobacterium-mediated plant callus infection was designed. Through the cooperation of connecting rods, threaded rods and clamping blocks, the movable plate can be flexibly adjusted to ensure full contact between the callus tissue and the bacterial solution. The bacterial solution is then absorbed by a grid-type filter and sterile filter paper, reducing the risk of damage and contamination during the transfer process.
It improves infection efficiency and uniformity, reduces the risk of callus damage and contamination, and ensures the vitality and regeneration capacity of callus.
Smart Images
Figure CN224513469U_ABST
Abstract
Claims
1. An apparatus for Agrobacterium infection of plant callus comprising a bottle (1) characterised in that: A connecting rod (7) is slidably connected to one side of the bottle body (1). The connecting rod (7) has a U-shaped structure. A movable plate (17) is fixedly connected to the bottom of the inner end of the connecting rod (7). The movable plate (17) is located inside the bottle body (1) and is slidably connected to the inner wall of the bottle body (1). Multiple sets of grid-type filter holes are opened on the movable plate (17). A threaded rod (8) is threadedly connected to the outer side of the connecting rod (7). A clamping block (9) is rotatably connected to the end of the threaded rod (8). The clamping block (9) is in contact with the outer side of the bottle body (1). The bottle cap (4) is connected to the top of the bottle cap (4) with a buckle. A pressure rod (12) is movably connected to the top of the bottle cap (4). A pressure plate (13) is installed at the bottom of the pressure rod (12). A limiting ring (14) is connected to the top of the bottle cap (4) to cooperate with the pressure plate (13). A slot (15) is opened in the limiting ring (14). Sterile filter paper is placed in the slot (15). Sterile liquid is filled in the bottle body (1). Multiple sets of callus tissue are placed on the top of the movable plate (17). The slot (15) is located below the pressure plate (13).
2. The apparatus according to claim 1, wherein the apparatus is characterized by: A pressure block (5) is fixedly installed on the top of the pressure rod (12). The pressure block (5) is located above the bottle cap (4). A spring (11) is fixedly connected to the bottom of the pressure block (5) and the top of the bottle cap (4). The pressure rod (12) is located at the center of the spring (11). A protective ring (6) is installed on the outer ring of the pressure block (5) at the top of the bottle cap (4).
3. The apparatus according to claim 2, wherein the apparatus is characterized by: The bottle body (1) has scale lines marked on the outside, and the clamp (9) is made of rubber. The bottle body (1) has a limiting groove (16) for use with the limiting ring (14).
4. The apparatus according to claim 3, wherein the apparatus is characterized by: Both the bottle cap (4) and the limiting ring (14) have slots (10) on one side for use with the connecting rod (7).
5. The apparatus of claim 4, wherein: the Agrobacterium is Agrobacterium tumefaciens. The bottle body (1) has a base (2) at the bottom, a rubber pad at the bottom of the base (2), and a timer (3) on one side of the base (2).