A disposable magnetic stirring bioreactor

Abstract

This invention relates to a disposable magnetic stirring bioreactor, which is discarded immediately after use, avoiding the risks of cleaning and cross-contamination, thus improving the safety and reliability of the bioreactor. It utilizes a magnetic stirrer beneath the reaction vessel to drive the stirring element within, eliminating the need for a mechanical transmission structure to pass through the vessel, ensuring a good seal and reducing the possibility of contamination. The impeller blades have polygonal locking grooves that connect with polygonal locking blocks on the edge of the stirring body, providing a secure, anti-rotation, and easy-to-install connection. The working length of the impeller can be indirectly adjusted through different mounting positions to accommodate reaction vessels of different diameters, greatly enhancing the adaptability and versatility of the device. The stackable design of the stirring elements increases the stirring coverage area and improves the stirring effect. Turbulence protrusions on the inner wall of the reaction vessel enhance the turbulence effect of the materials, improve stirring uniformity, and prevent materials from adhering to the inner wall of the vessel.

Description

Technical Field

[0001] This utility model relates to the field of bioreactor equipment technology, and more specifically, to a disposable magnetic stirring bioreactor device. Background Technology

[0002] In fields such as biopharmaceuticals, cell culture, and microbial fermentation, bioreactors are indispensable equipment. Traditional bioreactors are mostly made of reusable stainless steel, requiring strict cleaning and sterilization procedures before use. This process is not only cumbersome but also prone to cross-contamination due to incomplete cleaning, affecting experimental results or production quality.

[0003] With the development of biotechnology, disposable bioreactors have gradually attracted widespread attention due to their advantages such as no need for cleaning and avoidance of cross-contamination. However, existing disposable bioreactors have some shortcomings in terms of stirring. Some devices use mechanical stirring, which requires a complex transmission structure and can easily lead to a decrease in sealing performance and an increase in the risk of contamination. Other devices have a simple stirring substructure, which is not adaptable to stirring materials of different viscosities and quantities, resulting in poor stirring effect and affecting reaction efficiency and uniformity.

[0004] Therefore, developing a disposable magnetic stirring bioreactor with good sealing performance, excellent stirring effect, and the ability to avoid cross-contamination is of great practical significance. Utility Model Content

[0005] This invention provides a disposable magnetic stirring bioreactor, which aims to solve the problems of poor sealing, poor stirring effect and cross-contamination in existing bioreactors.

[0006] To achieve the above objectives, this utility model adopts the following technical solution: a disposable magnetic stirring bioreactor, comprising a reaction container, a stir bar, a sealing cap, and a magnetic stirrer. The reaction container is made of disposable sterile material, and a reaction chamber is formed inside the reaction container. The inner wall of the reaction container is provided with several turbulence protrusions. The stir bar is disposed in the reaction chamber and can stir the material in the reaction chamber. The sealing cap is sealed to the open end of the reaction container and is used to close the reaction chamber. The magnetic stirrer is located below the reaction container and is used to drive the stir bar to rotate. The stir bar includes a stirring base and a stirring body. The stirring body is disposed on the stirring base and is detachably connected to the stirring base. A permanent magnet is embedded inside the stirring base. The stirring body is provided with several blades and is detachably connected to the stirring body.

[0007] Furthermore, both the stir bar and the blades are made of polytetrafluoroethylene.

[0008] Furthermore, the impeller and the agitator are detachably connected via a snap-fit ​​structure.

[0009] Furthermore, the impeller and the agitator are detachably connected via a threaded structure.

[0010] Furthermore, the top of the stirring body is provided with a limiting protrusion, and the bottom of the stirring body is provided with a limiting groove that matches the limiting protrusion.

[0011] Furthermore, the top of the stirring base is provided with a connecting protrusion that matches the limiting groove.

[0012] Furthermore, the edge of the stirring body is provided with several regular polygonal snap-fit ​​blocks, and one end of the blade is provided with a snap-fit ​​groove that snaps into the snap-fit ​​blocks.

[0013] Furthermore, the reaction chamber is equipped with a temperature sensor and a viscosity sensor, and the signal output terminals of the temperature sensor and the viscosity sensor are connected to external monitoring equipment and the magnetic stirrer via wires.

[0014] Furthermore, the outer wall of the stirring base is provided with a flow guide groove.

[0015] Furthermore, a sealing gasket is provided at the connection between the sealing cap and the reaction vessel.

[0016] Compared with the prior art, the beneficial effects of this utility model are:

[0017] This invention utilizes a reaction vessel made of disposable sterile material, which is directly discarded after use, avoiding the cleaning process and the risk of cross-contamination, thus improving the safety and reliability of the biological reaction. A magnetic stirrer at the bottom of the reaction vessel drives the stir bar inside, eliminating the need for a mechanical transmission structure to pass through the vessel, ensuring a good seal of the reaction chamber and reducing the possibility of contamination. The impeller blades have regular polygonal locking grooves that connect with regular polygonal locking blocks on the edge of the stirrer body, providing a secure, anti-rotation, and easy-to-install connection. The working length of the impeller can also be indirectly adjusted through different installation positions to accommodate reaction vessels of different diameters, greatly improving the adaptability and versatility of the device. The stackable design of the stirrer body increases the stirring coverage area and enhances the stirring effect. Turbulence protrusions on the inner wall of the reaction vessel enhance the turbulence effect of the materials, improve stirring uniformity, and prevent materials from adhering to the inner wall of the vessel. Attached Figure Description

[0018] To more clearly illustrate the technical solutions of the embodiments of this utility model, the drawings used in the embodiments will be briefly introduced below. It should be understood that the following drawings only show some embodiments of this utility model and should not be regarded as a limitation of the scope. For those skilled in the art, other related drawings can be obtained from these drawings without creative effort.

[0019] Figure 1 This is a schematic diagram of the structure of this utility model;

[0020] Figure 2 This is a schematic diagram of the stir bar structure in this utility model;

[0021] Figure 3 This is an exploded schematic diagram of the stir bar in this utility model;

[0022] Figure 4 This is an exploded view of the stir bar in this utility model from another angle;

[0023] Figure 5 This is a schematic diagram of the stacked stirring bodies in this utility model.

[0024] Explanation of main component symbols

[0025] 100. Reaction vessel; 101. Turbulence protrusion;

[0026] 200. Stirring element; 210. Stirring base; 211. Flow guide groove; 212. Connecting protrusion; 213. Permanent magnet; 220. Stirring body; 221. Impeller; 222. Limiting protrusion; 223. Limiting groove; 224. Snap-fit ​​block; 225. Snap-fit ​​groove;

[0027] 300. Sealing cap; 301. Sealing gasket;

[0028] 400. Magnetic stirrer. Detailed Implementation

[0029] To make the objectives, technical solutions, and advantages of the embodiments of this utility model clearer, the technical solutions of the embodiments of this utility model will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only a part of the embodiments of this utility model, not all of them. All other embodiments obtained by those skilled in the art based on the embodiments of this utility model without creative effort are within the scope of protection of this utility model. Therefore, the following detailed description of the embodiments of this utility model provided in the accompanying drawings is not intended to limit the scope of the claimed utility model, but merely represents selected embodiments of this utility model. All other embodiments obtained by those skilled in the art based on the embodiments of this utility model without creative effort are within the scope of protection of this utility model.

[0030] In this utility model, unless otherwise explicitly specified and limited, the terms "installation," "connection," "joining," and "fixing," etc., should be interpreted broadly. For example, they can refer to a fixed connection, a detachable connection, or an integral part; they can refer to a mechanical connection or an electrical connection; they can refer to a direct connection or an indirect connection through an intermediate medium; they can refer to the internal communication of two components or the interaction between two components. Those skilled in the art can understand the specific meaning of the above terms in this utility model according to the specific circumstances.

[0031] In this invention, unless otherwise explicitly specified and limited, "above" or "below" the second feature can include direct contact between the first and second features, or contact between the first and second features through another feature between them. Furthermore, "above," "over," and "on top" of the second feature includes the first feature directly above or diagonally above the second feature, or simply indicates that the first feature is at a higher horizontal level than the second feature. "Below," "below," and "under" the second feature includes the first feature directly below or diagonally below the second feature, or simply indicates that the first feature is at a lower horizontal level than the second feature.

[0032] Example

[0033] like Figures 1 to 5 As shown, this utility model discloses a disposable magnetic stirring bioreactor, including a reaction container 100, a stir bar 200, a sealing cap 300, and a magnetic stirrer 400.

[0034] The reaction vessel 100 is made of disposable sterile materials, such as polyethylene, polypropylene, or polycarbonate. These materials have good biocompatibility and chemical stability and are easy to sterilize. The reaction vessel 100 has an internal reaction chamber to contain the reactants. The outer wall of the reaction vessel 100 is marked with graduations for easy observation and measurement of the material volume. The inner wall of the reaction vessel 100 has turbulent flow protrusions 101. When the material flows under the action of the stir bar 200, the turbulent flow protrusions 101 can break the laminar flow state of the material, forming more turbulence, thereby enhancing the mixing effect and improving the uniformity of stirring. Especially for materials with high viscosity, it can effectively prevent the material from adhering to the inner wall of the reaction vessel 100, improving reaction efficiency.

[0035] A stirrer 200 is disposed inside the reaction chamber and can stir the materials inside the reaction chamber. A sealing cap 300 is sealed to the open end of the reaction container 100 and is used to close the reaction chamber. A magnetic stirrer 400 is located below the reaction container 100 and is used to drive the stirrer 200 to rotate. The stirrer 200 includes a stirring base 210 and a stirring body 220. The stirring body 220 is disposed on the stirring base 210 and the stirring base 210 and the stirring body 220 are detachably connected. A permanent magnet 213 is embedded inside the stirring base 210. The stirring body 220 is provided with several blades 221 and the blades 221 are detachably connected to the stirring body 220. The blades 221 and the stirring body 220 can be detachably connected by a snap-fit ​​structure or a threaded structure.

[0036] Both the stirring base 210 and the stirring body 220 are made of polytetrafluoroethylene (PTFE). PTFE has advantages such as high temperature resistance, corrosion resistance, and non-stick properties, ensuring that the stirring body 220 operates stably during the reaction process.

[0037] The top of the aforementioned stirring body 220 is provided with a limiting protrusion 222, and the bottom of the stirring body 220 is provided with a limiting groove 223 that matches the limiting protrusion 222. Through the cooperation of the limiting protrusion 222 and the limiting groove 223, multiple stirring bodies 220 can be stacked. When the amount of reactant is large, stacking the stirring bodies 220 can increase the coverage of the stirring, make the material more uniformly stirred, and avoid insufficient reaction of local materials.

[0038] The top of the aforementioned stirring base 210 is provided with a connecting protrusion 212 that matches the limiting groove 223. The stirring base 210 and the stirring body 220 can be detachably connected by the cooperation of the connecting protrusion 212 and the limiting groove 223.

[0039] The aforementioned stirring body 220 has several regular polygonal snap-fit ​​blocks 224 evenly distributed along the circumference of the stirring body 220 at its edge. One end of the blade 221 has a snap-fit ​​groove 225 that snaps into the snap-fit ​​blocks 224. The snap-fit ​​blocks 224 and the snap-fit ​​groove 225 cooperate to achieve a detachable connection between the stirring body 220 and the blade 221. In this embodiment, the regular polygon can be a regular octagon or the like. When snapping, the snap-fit ​​blocks 224 are aligned with the snap-fit ​​groove 225 and inserted. The circumferential positioning is achieved by using the corners of the polygonal structure to prevent the blade 221 from rotating relative to the stirring body 220 during the stirring process, while ensuring the stability of the connection. In addition, it should be mentioned that multiple sides and corners of the regular polygon form multiple mounting positions. By selecting different mounting positions, the extension length of the blade 221 relative to the stirring body 220 can be indirectly adjusted. For example, in a reaction vessel 100 with a smaller diameter, the snap-fit ​​block 224 can be snapped into the inner side of the snap-fit ​​groove 225, which can shorten the working length of the blade 221 and prevent the blade 221 from colliding with the inner wall of the reaction vessel 100 when rotating. In a reaction vessel 100 with a larger diameter, the outer mounting position can be selected to keep the blade 221 at its maximum working length, enhance the stirring coverage of the material, and thus adapt to the usage requirements of reaction vessels 100 with different diameters, improving the versatility of the device. In addition, operators can also select blades 221 of different specifications and sizes to adapt to reaction vessels 100 with different diameters.

[0040] The reaction chamber is equipped with a temperature sensor and a viscosity sensor. The signal output terminals of the temperature sensor and the viscosity sensor are connected to external monitoring equipment and magnetic stirrer 400 through wires. The temperature sensor and the viscosity sensor can monitor the temperature and viscosity of the material in the reaction chamber in real time. The viscosity sensor transmits the monitored viscosity signal to the magnetic stirrer 400. The magnetic stirrer 400 automatically adjusts the speed according to the viscosity change. When the viscosity increases, the speed is automatically increased to ensure the stirring effect. When the viscosity decreases, the speed is automatically reduced to save energy, thus realizing intelligent stirring control.

[0041] The outer wall of the aforementioned mixing base 210 is provided with a flow guide groove 211, which can guide the flow of materials and improve the mixing efficiency.

[0042] A sealing gasket 301 is provided at the connection between the sealing cap 300 and the reaction vessel 100. The sealing gasket 301 is attached to the lower end face of the sealing cap 300, and the sealing cap 300 is placed over the opening of the reaction vessel 100. The sealing gasket 301 further improves the sealing performance of the device.

[0043] The usage process of this utility model is as follows: First, place the reaction vessel 100 on the magnetic stirrer 400; according to the characteristics of the material, the diameter of the reaction vessel 100 and the amount of material, select the installation position of the blade 221 (adjust the working length) and whether to stack the stirring body 220. If necessary, align the snap-fit ​​groove 225 of the blade 221 with the corresponding snap-fit ​​block 224 of the stirring body 220 and insert it to achieve installation. Stack the stirring body 220 through the limiting protrusion 222 and the limiting groove 223; then, add the required material into the reaction vessel 100; then, close the sealing cap 300 and open the valve. The magnetic stirrer 400 drives the stir bar 200 inside the reaction vessel 100 to rotate. The guide channel 211 guides the material flow, and the impeller 221 enhances the stirring effect. The turbulence protrusions 101 on the inner wall of the reaction vessel 100 break the laminar flow and form turbulent flow, which efficiently stirs the material. The magnetic stirrer 400 automatically adjusts the speed according to the material viscosity monitored by the viscosity sensor. During the reaction, the reaction temperature is monitored by the temperature sensor. After the reaction is completed, the magnetic stirrer 400 is turned off, the material is removed, and the reaction vessel 100 and other items are disposed of as disposable items.

[0044] The above are merely preferred embodiments of this utility model and are not intended to limit the scope of this utility model. Various modifications and variations can be made to this utility model by those skilled in the art. Any modifications, equivalent substitutions, or improvements made within the spirit and principles of this utility model should be included within the protection scope of this utility model.

Claims

1. A disposable magnetically stirred bioreactor, characterized in that, The device includes a reaction vessel, a stir bar, a sealing cap, and a magnetic stirrer. The reaction vessel is made of disposable sterile material and has a reaction chamber inside. The inner wall of the reaction vessel has several turbulence protrusions. The stir bar is located inside the reaction chamber and can stir the materials inside the reaction chamber. The sealing cap is sealed to the open end of the reaction vessel and is used to close the reaction chamber. The magnetic stirrer is located below the reaction vessel and is used to drive the stir bar to rotate. The stir bar includes a stirring base and a stirring body. The stirring body is mounted on the stirring base and is detachably connected to the stirring base. A permanent magnet is embedded inside the stirring base. The stirring body has several blades and is detachably connected to the stirring body.

2. The disposable magnetic stirring bioreactor apparatus of claim 1, wherein: Both the stir bar and the blades are made of polytetrafluoroethylene.

3. The disposable magnetic stirring bioreactor apparatus of claim 1, wherein: The blades and the agitator are detachably connected via a snap-fit ​​structure.

4. The disposable magnetic stirring bioreactor apparatus of claim 1, wherein: The blades and the agitator are detachably connected via a threaded structure.

5. The disposable magnetic stirring bioreactor apparatus of claim 1, wherein: The top of the stirring body is provided with a limiting protrusion, and the bottom of the stirring body is provided with a limiting groove that matches the limiting protrusion.

6. The disposable magnetic stirring bioreactor apparatus of claim 5, wherein: The top of the stirring base is provided with a connecting protrusion that matches the limiting groove.

7. The disposable magnetic stirring bioreactor apparatus of claim 1, wherein: The edge of the stirring body is provided with several regular polygonal snap-fit ​​blocks, and one end of the blade is provided with a snap-fit ​​groove that snaps into the snap-fit ​​blocks.

8. The disposable magnetic stirring bioreactor apparatus of claim 1, wherein: The reaction chamber is equipped with a temperature sensor and a viscosity sensor. The signal output terminals of the temperature sensor and the viscosity sensor are connected to external monitoring equipment and the magnetic stirrer via wires.

9. The disposable magnetic stirring bioreactor apparatus of claim 1, wherein; The outer wall of the stirring base is provided with a flow guide groove.

10. The disposable magnetic stirring bioreactor apparatus of claim 1, wherein; A sealing gasket is provided at the connection between the sealing cap and the reaction vessel.

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