一种PCR检测装置

Abstract

本实用新型涉及一种PCR检测装置，包括外壳，以及设置在外壳内的电控板和微流控芯片；电控板上集成有主控芯片、温度控制ARM芯片和温度电极柱阵列；微流控芯片相对于电控板匹配安装；微流控芯片包括由下至上依次层叠的第一基板、第二基板和第三基板；第一基板上集成有温度感应元件，第二基板上且与温度感应元件对应的位置上集成有微型加热元件，第三基板上集成有反应腔，反应腔的位置与微型加热元件的位置对应，微型加热元件与温度电极柱阵列电连接，温度控制ARM芯片根据温度信号控制微型加热元件通电加热。本实用新型通过基板内嵌温度感应元件和微型加热元件，提高了温度循环的升温速率，缩短PCR反应过程的时间。

Claims

1. A PCR detection device, characterized by: It includes a housing, and an electronic control board and a microfluidic chip disposed within the housing; The electronic control board integrates a main control chip, a temperature control ARM chip, and a temperature electrode column array; the main control chip is electrically connected to the temperature control ARM chip, and the temperature control ARM chip is electrically connected to the temperature electrode column array. The microfluidic chip is mounted relative to the electronic control board; the microfluidic chip includes a first substrate, a second substrate, and a third substrate stacked sequentially from bottom to top; a temperature sensing element is integrated on the first substrate, a micro heating element is integrated on the second substrate at a position corresponding to the temperature sensing element, and a reaction chamber is integrated on the third substrate, the position of which corresponds to the position of the micro heating element. The temperature sensing element is electrically connected to the temperature electrode array to transmit the sensed temperature signal to the temperature control ARM chip through the temperature electrode array; the micro heating element is electrically connected to the temperature electrode array, and the temperature control ARM chip controls the micro heating element to be powered on and heated according to the temperature signal.

2. The PCR detection device of claim 1, wherein: A fan is also provided inside the housing. The fan is electrically connected to the temperature electrode column array. The temperature control ARM chip controls the fan to power on and run for heat dissipation based on the temperature signal.

3. The PCR detection device of claim 1, wherein: The control board also integrates a temperature-controllable switch array and a power supply. The temperature control ARM chip is electrically connected to the control terminal group of the temperature-controllable switch array. The two switch terminal groups of the temperature-controllable switch array are respectively electrically connected to the power supply and the temperature electrode column array.

4. The PCR detection device of claim 1, wherein: The second substrate consists of two stacked substrates, each of which integrates the micro heating element.

5. The PCR detection device of claim 1, wherein: The electronic control board also integrates a power supply, a droplet control high-voltage chip, a droplet control controllable switch array, and a droplet control electrode column array; the main control chip is also electrically connected to the droplet control high-voltage chip, the droplet control high-voltage chip is electrically connected to the control terminal group of the droplet control controllable switch array, and the two switch terminal groups of the droplet control controllable switch array are respectively electrically connected to the power supply and the droplet control electrode column array; The third substrate also integrates a microchannel electrode array that is configured to cooperate with the reaction chamber and is used for electrowetting drive. The microchannel electrode array contacts the droplet control electrode column array to achieve electrical connection.

6. The PCR detection device according to claim 1, characterized in that: The control board also integrates a power supply, a light source control PWM chip, a light source controllable switch, a light source, and a photovoltaic cell; the main control chip is also electrically connected to the light source control PWM chip and the photovoltaic cell; the light source control PWM chip is electrically connected to the control terminal of the light source controllable switch, and the two switch terminals of the light source controllable switch are respectively electrically connected to the power supply and the light source; the reaction chambers on the third substrate are respectively configured to cooperate with the light source and the photovoltaic cell.

7. The PCR detection device of claim 1, wherein: A ring of radial star-shaped electrodes is integrated on the third substrate and around the reaction chamber.

8. The PCR detection device of claim 1, wherein: The outer casing is also provided with a display screen and / or an optical window. The display screen is electrically connected to the main control chip and is used to display the detection results. The optical window is directly facing the reaction chamber.

9. The PCR detection device of claim 1, wherein: The edge of the third substrate is integrated with a conductive electrode array electrically connected to the microchannel electrode array, the micro heating element, and the temperature sensing element; a first hydrophobic insulating film is deposited on the area of ​​the third substrate where the reaction chamber and the microchannel electrode array are integrated; a frame-type signal transmission board is stacked on the third substrate, surrounding the first hydrophobic insulating film, and a signal transmission electrode array is integrated on the signal transmission board, the signal transmission electrode array corresponding to and in close contact with the conductive electrode array; a second hydrophobic insulating film is deposited inside the frame of the signal transmission board and on the first hydrophobic insulating film; a hydrophobic conductive film covering the second hydrophobic insulating film is deposited on the frame of the signal transmission board.

10. The PCR detection device of claim 1, wherein: The first substrate, the second substrate, and the third substrate are specifically glass fiber board, epoxy resin board, or polyimide board.

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