一种便携式粪菌分离移植装置
The portable fecal microbiota separation and transplantation device employs multiple technical methods to achieve efficient separation and purification of target microbiota in fecal samples, solving the problems of complex operation and poor portability of existing devices and meeting clinical needs.
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- WEST CHINA HOSPITAL SICHUAN UNIV
- Filing Date
- 2025-08-01
- Publication Date
- 2026-07-17
AI Technical Summary
Existing fecal microbiota separation and transplantation devices are complex to operate, bulky, difficult to carry, and inconvenient to operate on-site. They are also prone to contamination or reduced microbial activity due to human factors.
A portable fecal microbiota separation and transplantation device was designed, including an inlet channel, a horizontal column, a filter material carrier, a resuspension and preservation tank, a primary processing chamber, a filter, an adsorption chamber, a centrifuge chamber, a washer, and a concentration and resuspension chamber. Through a variety of technical means such as stirring, filtration, adsorption, centrifugation, washing, and concentration, the device achieves efficient separation and purification of target microbiota in fecal samples.
It achieves efficient isolation and purification of target microbial communities in fecal samples. The device is compact, portable and easy to operate, and suitable for various scenarios. The prepared microbial suspension is suitable for preservation or transplantation, meeting clinical needs.
Smart Images
Figure CN224513490U_ABST
Abstract
Claims
1. A portable device for fecal microbiota transplantation, characterized in that, It includes an inlet channel (1), a horizontal column (3), a filter carrier (6), a resuspension storage tank (7), a primary processing chamber (2), a filter (9), an adsorption chamber (10), a centrifuge chamber (11), a scrubber (12), and a concentration and resuspension chamber (13); the inlet channel (1) is connected to the top of the horizontal column (3), the primary processing chamber (2) is located below the inlet channel (1), the horizontal column (3) has a guide channel inside, the guide channel is connected to the filter carrier (6) and the resuspension storage tank (7) respectively, the filter (9) is connected above the filter carrier (6), the adsorption chamber (10) and the centrifuge chamber (11) are connected above the resuspension storage tank (7) in sequence, and the scrubber (12) and the concentration and resuspension chamber (13) are arranged inside the resuspension storage tank (7) in sequence.
2. The apparatus of claim 1, wherein, The primary processing chamber (2) is equipped with a stirrer and a sterile buffer salt solution storage unit. The stirrer mixes the fecal sample with the sterile buffer salt solution at a fixed speed.
3. The apparatus of claim 2, wherein, The stirring speed range is 500 rpm to 800 rpm.
4. The apparatus of claim 1, wherein, The filter (9) is composed of multiple layers of filter membranes with different pore sizes, which are, from top to bottom, a coarse filter membrane, a fine filter membrane and an ultrafiltration membrane. The pore size range of each filter membrane is 500 micrometers to 200 micrometers, 200 micrometers to 50 micrometers and 50 micrometers to 10 micrometers, respectively.
5. The apparatus of claim 1, wherein, The adsorption chamber (10) is filled with a specific adsorption material, the surface of which is modified with a specific ligand, and a flow controller is provided at the outlet of the adsorption chamber (10).
6. The apparatus of claim 5, wherein, The flow controller has an adjustment range of 1 ml / min to 10 ml / min.
7. The apparatus of claim 1, wherein, The centrifuge chamber (11) is equipped with multiple sets of adjustable speed centrifuge rotors, with the rotor speed range being 1000 rpm to 10000 rpm. The bottom of the centrifuge chamber (11) is equipped with a slag discharge port.
8. The apparatus of claim 1, wherein, The washer (12) is equipped with a spray head connected to a buffer storage unit. The buffer solution is a phosphate buffer with a pH range of 6.8 to 7.4 and an ionic strength of 0.1 mol per liter.