一种微流控芯片

Abstract

本实用新型涉及细胞封装计数的技术领域，特别是涉及一种微流控芯片。本申请的微流控芯片上设置有样本孔、废液孔和稀释孔，微流控芯片内设置有样本微流道、废液流道和稀释微流道，样本孔与样本微流道连通，废液孔与废液流道连通，稀释孔与稀释微流道连通；微流控芯片内还设置有稀释腔和拍照观察腔。本申请的微流控芯片，通过集成化设计，巧妙设计稀释腔和拍照观察腔的位置和连接关系，并在稀释腔中设置细胞过滤层，无需额外的外部过滤设备，成功将样本稀释、过滤和观察等步骤整合到一个微流控芯片中，大大简化了操作流程，大大提高了细胞计数的效率和准确性。

Claims

1. A microfluidic chip, characterized by, The microfluidic chip is provided with a sample port, a waste liquid port and a dilution port. The microfluidic chip is provided with a sample microchannel, a waste liquid channel and a dilution microchannel. The sample port is connected to the sample microchannel, the waste liquid port is connected to the waste liquid channel and the dilution port is connected to the dilution microchannel. The microfluidic chip is also equipped with a dilution chamber and an image observation chamber; Both the dilution microchannel and the sample microchannel are connected to the inlet of the dilution chamber; The inlet of the photographic observation chamber is connected to the outlet of the dilution chamber, and the outlet of the photographic observation chamber is connected to the waste liquid flow channel; The dilution chamber is equipped with a cell filtration layer.

2. The microfluidic chip of claim 1, wherein, The microfluidic chip includes a first cover plate and a second cover plate that are attached to each other. The first cover plate is provided with a first sinking groove, and the second cover plate is provided with a second sinking groove. The first sinking groove and the second sinking groove are arranged opposite to each other and form the dilution chamber.

3. The microfluidic chip of claim 2, wherein, The cell filter layer is sandwiched between the first settling tank and the second settling tank.

4. The microfluidic chip of claim 3, wherein, Both the first sedimentation tank and the second sedimentation tank are provided with micropillar structures, each micropillar structure including a plurality of spaced protrusions, and the cell filter layer is sandwiched between the micropillar structures of the first sedimentation tank and the second sedimentation tank.

5. The microfluidic chip of claim 3, wherein, The first settling tank or the second settling tank is provided with a clearance ring groove for avoiding the cell filter layer, and the edge of the cell filter layer is embedded in the clearance ring groove.

6. The microfluidic chip according to claim 5, characterized in that, The shape of the avoidance ring groove matches the shape of the cell filter layer so that the edge of the cell filter layer is embedded in the avoidance ring groove.

7. The microfluidic chip of claim 1, wherein, The microfluidic chip is also provided with dye orifices, and dye microchannels and mixing microchannels are provided inside the microfluidic chip, with the dye orifices communicating with the dye microchannels. The outlet of the dilution chamber and the dye microchannel are both connected to the mixing microchannel to stain the sample in the mixing microchannel. The inlet of the imaging observation chamber and the outlet of the dilution chamber are connected through the mixing microchannel.

8. The microfluidic chip of claim 7, wherein, The mixing microchannel includes multiple mixing units connected in sequence, which are used to guide the liquid to achieve splitting and converging.

9. The microfluidic chip of claim 2, wherein, The photographic observation chamber is equipped with a lens focusing feature and a single-cell fluid channel; There is a preset height difference between the single-cell fluid channel and the lens focusing feature. The lens focusing feature is used to determine the lens focal length. The diameter of the single-cell fluid channel is set according to the size of the cells to be counted in the sample.

10. The microfluidic chip of claim 9, wherein, The lens focusing feature is a crosshair or a circle.

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