一种脑胶质瘤类器官的快速培养装置
By designing a rapid culture device for glioma organoids, which uses placement rods and sealing plates to simulate the three-dimensional environment in vivo, the shortcomings of traditional two-dimensional culture methods are overcome, enabling rapid culture and efficient drug development.
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- SHANGHAI SIXIN PHARM TECH CO LTD
- Filing Date
- 2025-08-20
- Publication Date
- 2026-07-17
AI Technical Summary
Existing organoid culture technologies mostly employ traditional two-dimensional culture methods, which are difficult to simulate the three-dimensional environment in vivo. Furthermore, conventional organoid dispensing methods have long cycles and complex culture systems, making it difficult to achieve large-scale, high-throughput screening.
A rapid culture device for glioma organoids was designed. By placing a drop of gel on the placement rod, half of it is in contact with the flowing culture medium and the other half with the air. Combined with the design of a sealed cover and evaporation holes, the device simulates the three-dimensional environment in vivo and controls the evaporation rate to reduce dust ingress.
This technology enables a significant reduction in the culture cycle in a simulated three-dimensional environment, improving drug development efficiency and reducing the risks of culture variability and dust contamination.
Smart Images
Figure CN224513514U_ABST
Abstract
Claims
1. A rapid culture device for brain glioma organoids, comprising a culture plate (1), characterized in that: The culture plate (1) has several rectangularly distributed placement holes (2) on its upper side. The inner side of each of the several placement holes (2) is fixedly connected to a placement mechanism (3). Four rectangularly distributed flow tubes (4) are fixedly connected to the left and right sides of the culture plate (1). A sealing mechanism (5) overlaps on the upper side of the culture plate (1). A rubber pad (6) is fixedly connected to the lower side of the culture plate (1). The placement mechanism (3) includes a placement rod (31), the lower side of which overlaps the bottom of the inner side of the placement hole (2), and a number of evenly distributed clamping plates (32) are fixedly connected to the bottom of the inner side of the placement hole (2). The outer side of the placement rod (31) is movably sleeved between the clamping plates (32).
2. The rapid culture device of a brain glioma organoid according to claim 1, characterized in that: Each of the several said card plates (32) has a claw (33) fixedly connected to one side of each other.
3. The rapid culture device of a brain glioma organoid according to claim 1, characterized in that: An arc-shaped groove is provided on the upper side of the mounting rod (31).
4. The rapid culture device for brain glioma organoids of claim 1, wherein: The sealing mechanism (5) includes a sealing cover plate (51), the lower side of which overlaps the upper side of the culture plate (1), and a number of rectangularly distributed sealing nets (53) are fixedly connected to the inner side of the side wall of the sealing cover plate (51). A number of annularly distributed evaporation holes (52) are opened on the inner side of the number of placement holes (2).
5. The rapid culture device for brain glioma organoids according to claim 4, characterized in that: The inner side of the culture plate (1) is movably sleeved with several horizontally distributed pull rods (54), and the inner side of the culture plate (1) is movably sleeved with several horizontally distributed springs (55), and the springs (55) are located on the upper side of the circular plate below the pull rods (54). The upper side of the pull rods (54) is fixedly connected with a pressure plate (56).
6. The rapid culture device for glioma organoids according to claim 5, characterized in that: The upper middle part of the sealing cover plate (51) has several horizontally distributed slots (57).
7. The rapid culture device of a brain glioma organoid according to claim 6, characterized in that: A fixing plate (58) is fixedly connected to the upper side of the culture plate (1) near the four corners.