一种模拟气体交换的肺癌类器官与成纤维细胞共培养装置
By using a co-culture device for lung cancer organoids and fibroblasts that simulates gas exchange, and by employing a gas-driven mechanism and a waste liquid discharge system, the problems of lack of physiological structure and cumbersome waste liquid cleaning in lung cancer organoid models have been solved, achieving higher physiological relevance and experimental convenience.
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- SHANGHAI SIXIN PHARM TECH CO LTD
- Filing Date
- 2025-08-20
- Publication Date
- 2026-07-17
AI Technical Summary
Existing lung cancer organoid models lack the tumor microenvironment and lung physiological structure, making it difficult to simulate intercellular interactions. They have low survival rates and purity, and waste liquid disposal is cumbersome and prone to contamination, making it difficult to simulate the interaction between immune cells and tumor tissue in the tumor microenvironment.
Design a co-culture device for lung cancer organoids and fibroblasts that simulates gas exchange. The device uses a gas-driven mechanism to control an elastic diaphragm to simulate alveolar breathing. It also includes a waste liquid discharge pipe and a single-phase drainage membrane to simulate alveolar movement and rapidly discharge waste liquid, thereby enhancing the physiological relevance of the tumor microenvironment and the convenience of experimental operation.
This improved the physiological relevance of co-culturing lung cancer organoids with fibroblasts, enhanced the activity and structural integrity of the organoids, simplified the waste disposal process, and improved the convenience and reliability of experimental operations.
Smart Images
Figure CN224513515U_ABST
Abstract
Claims
1. A device for co-culturing lung cancer organoids with fibroblasts mimicking gas exchange, comprising a co-culturing mechanism (1), characterized in that: A culture medium supply mechanism (3) is provided on the right side of the co-culture mechanism (1), and a gas driving mechanism (2) is provided on the right side of the culture medium supply mechanism (3). The co-culture mechanism (1) includes an organoid chip body (11). Two elastic diaphragms (12) are fixedly installed between the upper and lower sides of the inner wall of the organoid chip body (11). A biomembrane (17) is fixedly connected between the opposite surfaces of the two elastic diaphragms (12). A portal-shaped gas supply and extraction pipe (13) is fixedly connected to the left and right sides of the front end of the organoid chip body (11) and passes through its inner cavity. The two vertical points of the portal-shaped gas supply and extraction pipe (13) are perpendicular to the organoid chip. The penetration point of the inner cavity of the body (11) is located on the opposite side of the two elastic diaphragms (12). The top of the horizontal part of the gate-shaped gas delivery and extraction tube (13) is fixedly connected to the main gas tube (14). The middle of the right end of the organoid chip body (11) is fixedly connected to the culture medium delivery tube (15) and the cell delivery tube (16). The left end of the culture medium delivery tube (15) penetrates to the bottom of the biomembrane (17) between the opposite surfaces of the two elastic diaphragms (12) in the inner cavity of the organoid chip body (11). The left end of the cell delivery tube (16) penetrates to the top of the biomembrane (17) between the opposite surfaces of the two elastic diaphragms (12) in the inner cavity of the organoid chip body (11).
2. The lung cancer organoid co-culture device for simulating gas exchange according to claim 1, wherein: The organoid chip body (11) has waste liquid discharge pipes (18) that extend to the upper and lower sides of the left end, respectively.
3. The device for co-culturing lung cancer organoids with fibroblasts to simulate gas exchange of claim 2, wherein: The other end of each of the two waste liquid discharge pipes (18) is connected to a waste liquid bottle.
4. The device for co-culturing lung cancer organoids with fibroblasts to simulate gas exchange of claim 1, wherein: The gas drive mechanism (2) includes an air pump (21), the output end of which is fixedly connected to a solenoid valve (22), the output end of which is fixedly connected to the end of the main air pipe (14) away from the organoid chip body (11), and a controller (23) is fixedly installed on the top of the air pump (21).
5. The device for co-culturing lung cancer organoids with fibroblasts to simulate gas exchange of claim 1, wherein: The culture medium supply mechanism (3) includes a peristaltic pump (31) and a storage bottle (34). A pump head (32) is fixedly installed at the front end of the peristaltic pump (31). The pump head (32) has a pipe mounting groove (33) that runs through the left and right sides. The end of the culture medium delivery pipe (15) away from the organoid chip body (11) passes through the pipe mounting groove (33) to the right side of the pump head (32) and is fixedly connected to the inner cavity of the storage bottle (34).
6. The device for co-culturing lung cancer organoids with fibroblasts to simulate gas exchange of claim 5, wherein: The top of the liquid storage bottle (34) is fixedly connected to a threaded liquid inlet, and a threaded cap (35) is threadedly installed on the outer wall of the threaded liquid inlet.
7. The device for co-culturing lung cancer organoids with fibroblasts to simulate gas exchange of claim 4, wherein: The controller (23) controls the air pump (21) to inflate and evacuate at a frequency of 0.1 to 0.5 Hz via the solenoid valve (22) to simulate the alveolar breathing rhythm.