A polyacrylamide gel protein recovery device

Abstract

This utility model discloses a polyacrylamide gel protein recovery device, belonging to the technical field of polyacrylamide gel protein recovery. It includes a housing, with a fixed cylinder fixedly connected to the inner bottom wall of the housing. A telescopic cylinder is slidably connected inside the fixed cylinder, and a screw is threadedly connected inside the telescopic cylinder. A servo motor is installed at the bottom of the housing, with its output end penetrating the housing and fixedly connected to the bottom end of the screw. Clamping frames are fixedly connected to both sides of the telescopic cylinder, and several sets of elastic clips are fixedly connected to the upper surface of each clamping frame. One side of each set of elastic clips is inclined. This polyacrylamide gel protein recovery device ensures that the dialysis bag opening remains sealed during the polyacrylamide gel protein recovery electrophoresis process, preventing the decomposed protein from leaking out through gaps in the dialysis bag opening and ensuring the reliability of protein recovery.

Description

Technical Field

[0001] This invention belongs to the field of polyacrylamide gel protein recovery technology, and particularly relates to a polyacrylamide gel protein recovery device. Background Technology

[0002] Polyacrylamide gel refers to cross-linked polyacrylamide, which is produced by polymerization of acrylamide monomers with methylenebisacrylamide as a cross-linking agent, catalyzed by tetramethylethylenediamine and initiated by free radicals. In order to recover the protein in polyacrylamide gel, a recovery device is used to separate the protein in the polyacrylamide gel by electrophoresis, using a catalyst and a dialysis bag.

[0003] The existing utility model with authorization announcement number CN210090131U discloses a polyacrylamide gel protein recovery device, including a fixing device, which includes at least two fixing strips, and the horizontal distance between each pair of fixing strips is adjustable; the upper surface of the fixing strips is provided with at least one protrusion; the fixing device also includes a clamp.

[0004] The above technical solution allows for simple and flexible adjustment of the horizontal distance between the two fixing strips, facilitating the fixing of dialysis bags of different lengths and widths. This also facilitates the purification of target protein strips of different lengths and widths and enables efficient recovery of samples of varying amounts. However, in the above technical solution, the polyacrylamide gel protein recovery device only uses material clips to fix the opening of the dialysis bag. This makes it easy for the dialysis bag opening to leak out due to poor sealing. Consequently, proteins may also leak out through the gaps in the bag opening during electrophoresis, greatly affecting the reliability of protein recovery in the polyacrylamide gel protein recovery device.

[0005] To address this issue, we propose a polyacrylamide gel protein recovery device. Utility Model Content

[0006] The purpose of this invention is to solve the problem in the prior art that the dialysis bag opening cannot be sealed more effectively, which leads to the leakage of buffer solution inside the dialysis bag due to poor sealing. This, in turn, causes proteins to leak out through the gaps in the bag opening during electrophoresis. Therefore, a polyacrylamide gel protein recovery device is proposed.

[0007] To achieve the above objectives, the present invention adopts the following technical solution: A polyacrylamide gel protein recovery device includes a housing. A fixed cylinder is fixedly connected to the inner bottom wall of the housing. A telescopic cylinder is slidably connected inside the fixed cylinder. A screw is threadedly connected inside the telescopic cylinder. A servo motor is installed below the housing. The output end of the servo motor passes through the housing and is fixedly connected to the bottom end of the screw. Clamping frames are fixedly connected to both sides of the telescopic cylinder. Several sets of elastic clips are fixedly connected to the upper surface of each clamping frame. One side of each set of elastic clips is inclined. A dialysis bag is clamped inside one set of elastic clips. Two rubber air bladders are fixedly connected to the inner walls of each clamping frame. A pressure relief valve is fixedly connected to the upper surface of each rubber air bladder. The air outlet of each pressure relief valve extends to the top of the clamping frame. An air suction pump is fixedly connected to the front of the housing. An electromagnetic three-way valve is fixedly connected to the output end of the air suction pump. A hose is fixedly connected to the second port of the electromagnetic three-way valve. A connecting pipe is fixedly connected to the other end of the hose. The outer surface of each rubber air bladder is fixedly connected to the connecting pipe.

[0008] Preferably, four support legs are fixedly connected to the bottom surface of the box, and electrophoresis power supplies are fixedly embedded on both sides of the box.

[0009] Preferably, a rubber sealing ring is fixedly connected to the upper surface of the fixed cylinder, and the inner wall of the rubber sealing ring is in contact with the outer surface of the telescopic cylinder.

[0010] Preferably, an anti-detachment ring is fixedly connected to the outer surface of the screw, and the upper surface of the anti-detachment ring is in contact with the inner top wall of the telescopic cylinder.

[0011] Preferably, a stabilizing base is fixedly connected to the left side of the servo motor, and the upper surface of the stabilizing base is fixedly connected to the bottom surface of the housing.

[0012] Preferably, both the front and back sides of the two clamping frames are fixedly connected with reinforcing ribs, and one side of each reinforcing rib is fixedly connected to the outer surface of the telescopic cylinder.

[0013] Preferably, the bottom surfaces of the two clamping frames are fixedly connected with a plurality of limiting plates, the plurality of limiting plates are inclined at a certain angle, and the outer surfaces of the plurality of limiting plates are provided with vent holes arranged at equal intervals.

[0014] Preferably, the air intake end of the air pump is fixedly connected to an air intake hood, and the back of the air intake hood is fixedly connected to the front of the housing.

[0015] In summary, the technical effects and advantages of this utility model are as follows: The dialysis bag is initially secured by elastic clips. Then, the suction provided by the suction pump draws outside air into the rubber bladder through the opened electromagnetic three-way valve, hose, and connecting tube. As the rubber bladder expands, it further compresses the opening of the dialysis bag from both sides, ensuring the bag's seal during polyacrylamide gel protein recovery. This prevents the buffer solution inside the dialysis bag from leaking out, ensuring the bag opening remains sealed throughout the electrophoresis process and preventing the decomposed proteins from leaking out through gaps. This guarantees the reliability of protein recovery using the polyacrylamide gel protein recovery device. Attached Figure Description

[0016] Figure 1 This is a three-dimensional structural diagram of the box body of this utility model; Figure 2 This is a three-dimensional cross-sectional structural diagram of the box body of this utility model; Figure 3 This is a cross-sectional three-dimensional structural schematic diagram of the fixed cylinder and telescopic cylinder of this utility model; Figure 4 This is a three-dimensional structural diagram of the clamping frame of this utility model after it has been unfolded.

[0017] In the diagram: 1. Box body; 2. Fixed cylinder; 3. Telescopic cylinder; 4. Screw; 5. Servo motor; 6. Clamping frame; 7. Elastic clamp; 8. Dialysis bag; 9. Rubber air bladder; 10. Pressure relief valve; 11. Suction pump; 12. Solenoid three-way valve; 13. Hoses; 14. Connecting pipe; 16. Support leg; 17. Electrophoresis power supply; 18. Rubber sealing ring; 19. Anti-detachment ring; 20. Stabilizing base; 21. Reinforcing rib; 22. Limiting plate; 23. Vent hole; 24. Air inlet hood. Detailed Implementation

[0018] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments of the present utility model. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments.

[0019] Reference Figure 1-4 A polyacrylamide gel protein recovery device includes a housing 1. A fixed cylinder 2 is fixedly connected to the inner bottom wall of the housing 1. A telescopic cylinder 3 is slidably connected inside the fixed cylinder 2. Four support legs 16 are fixedly connected to the bottom surface of the housing 1. Electrophoresis power supply units 17 are fixedly embedded on both sides of the housing 1. The support legs 16 can support the device and keep it at a suitable height. The electrophoresis power supply units 17 can provide the positive and negative current required for electrophoresis to the buffer solution in the housing 1, ensuring the smooth operation of polyacrylamide gel electrophoresis protein recovery.

[0020] The telescopic cylinder 3 is internally threaded with a screw 4. A servo motor 5 is installed at the bottom of the housing 1. The output end of the servo motor 5 passes through the housing 1 and is fixedly connected to the bottom end of the screw 4. A rubber sealing ring 18 is fixedly connected to the upper surface of the fixed cylinder 2. The inner wall of the rubber sealing ring 18 is in contact with the outer surface of the telescopic cylinder 3. The rubber sealing ring 18 can seal the gap between the fixed cylinder 2 and the telescopic cylinder 3 to prevent the buffer solution from entering the interior of the telescopic cylinder 3 through the gap.

[0021] Both sides of the telescopic cylinder 3 are fixedly connected to clamping frames 6. Several sets of elastic clamping pieces 7 are fixedly connected to the upper surface of the two clamping frames 6. One side of each set of elastic clamping pieces 7 is an inclined surface. An anti-detachment ring 19 is fixedly connected to the outer surface of the screw 4. The upper surface of the anti-detachment ring 19 is in contact with the inner top wall of the telescopic cylinder 3. The anti-detachment ring 19 can limit the maximum upward movement of the telescopic cylinder 3 and prevent the telescopic cylinder 3 from becoming loose from the screw 4.

[0022] One set of elastic clips 7 has a dialysis bag 8 inside, and two rubber airbags 9 are fixedly connected to the inner walls of the two clamping frames 6. A stabilizing seat 20 is fixedly connected to the left side of the servo motor 5. The upper surface of the stabilizing seat 20 is fixedly connected to the bottom surface of the box 1. The stabilizing seat 20 can stabilize the position of the servo motor 5 and ensure that the servo motor 5 can smoothly drive the screw 4 to rotate.

[0023] Each rubber airbag 9 has a pressure relief valve 10 fixedly connected to its upper surface. The air outlet of each pressure relief valve 10 extends through to the top of the clamping frame 6. The front and back of the two clamping frames 6 are fixedly connected with reinforcing ribs 21. One side of each reinforcing rib 21 is fixedly connected to the outer surface of the telescopic cylinder 3. The reinforcing ribs 21 can increase the tightness of the connection between the clamping frame 6 and the telescopic cylinder 3, making the clamping frame 6 less prone to loosening and improving the pressure resistance of the clamping frame 6.

[0024] An air pump 11 is fixedly connected to the front of the housing 1. The output end of the air pump 11 is fixedly connected to an electromagnetic three-way valve 12. Several limiting plates 22 are fixedly connected to the bottom surfaces of the two clamping frames 6. The limiting plates 22 are all at a certain angle. The outer surfaces of the limiting plates 22 are provided with equidistant air vents 23. The limiting plates 22 can restrict the position of the dialysis bag 8, prevent the dialysis bag 8 from floating upward and affecting the electrophoresis effect of the polyacrylamide gel inside. The air vents 23 can also ensure that the buffer solution inside the housing 1 is in contact with the dialysis bag 8.

[0025] The second port of the electromagnetic three-way valve 12 is fixedly connected to a hose 13, and the other end of the hose 13 is fixedly connected to a connecting pipe 14. The outer surface of each rubber airbag 9 is fixedly connected to the connecting pipe 14. The air intake end of the air pump 11 is fixedly connected to an air intake cover 24. The back of the air intake cover 24 is fixedly connected to the front of the housing 1. The air intake cover 24 can filter the outside air without affecting the smooth air intake of the air pump 11, preventing external impurities from entering the air pump 11.

[0026] The working principle of this utility model is as follows: In use, firstly, the servo motor 5, the suction pump 11, the electromagnetic three-way valve 12, and the electrophoresis power supply 17 are connected to an external power source and a controller. When it is necessary to recover proteins from electrophoresis of polyacrylamide gel, the polyacrylamide gel to be decomposed and some buffer solution are then placed into the dialysis bag 8. Then, through the opening of the clamping frame 6 and the elasticity of the elastic clip 7, the position of the dialysis bag 8 can be initially clamped and fixed from the side. Then, using the suction provided by the suction pump 11, outside air can be sent into the rubber gas cylinder through the opened electromagnetic three-way valve 12, the hose 13, and the connecting tube 14. As the rubber bladder 9 expands, it can further squeeze the opening of the dialysis bag 8 from both sides, thereby ensuring the sealing of the opening of the dialysis bag 8 during the polyacrylamide gel protein recovery process. This prevents the buffer solution inside the dialysis bag 8 from leaking outwards, ensuring that the opening of the dialysis bag 8 remains sealed during the polyacrylamide gel protein recovery electrophoresis process. This prevents the decomposed proteins from leaking outwards through the gaps in the opening of the dialysis bag 8, ensuring the reliability of protein recovery in the polyacrylamide gel protein recovery device. Furthermore, the pressure relief valve 10 can be used to release excess air pressure inside the rubber bladder 9, preventing the rubber bladder 9 from over-expanding. After the dialysis bag 8 is clamped and sealed, the servo motor 5 is rotated in conjunction with the screw 4 to move the telescopic cylinder 3 downwards until the dialysis bag 8 is completely immersed in the buffer solution inside the chamber 1. At this time, the electrophoresis power supply 17 can provide the positive and negative current required for the protein recovery of the polyacrylamide gel. The negatively charged proteins will migrate towards the positive electrode, leave the gel and enter the buffer solution inside the dialysis bag 8. After electrophoresis, the proteins remain in the buffer solution inside the dialysis bag 8. After electroelution, a reverse current is applied through the electrophoresis power supply 17 to allow the sample adsorbed on the dialysis bag 8 to enter the buffer solution. This achieves the purpose of recovering the specific protein macromolecules required in the gel. After the protein recovery of the polyacrylamide gel is completed, the servo motor 5 is reversed to move the telescopic cylinder 3, which in turn moves the clamping frame 6 and the dialysis bag 8 upwards. Then, the electromagnetic three-way valve 12 is controlled to open the third port, allowing the gas inside the rubber air bag 9 to be discharged outwards, and the dialysis bag 8 can be removed from the elastic clamp 7.

[0027] In the description of this utility model, it should be understood that the terms "center", "longitudinal", "lateral", "length", "width", "thickness", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", "clockwise", "counterclockwise", etc., indicating the orientation or positional relationship are based on the orientation or positional relationship shown in the accompanying drawings, and are only for the convenience of describing this utility model and simplifying the description, and are not intended to indicate or imply that the device or component referred to must have a specific orientation, or be constructed and operated in a specific orientation, and therefore should not be construed as a limitation of this utility model.

[0028] Furthermore, the terms "first" and "second" are used for descriptive purposes only and should not be construed as indicating or implying relative importance or implicitly specifying the number of technical features indicated. Thus, a feature defined as "first" or "second" may explicitly or implicitly include one or more of that feature. In the description of this utility model, "a plurality of" means two or more, unless otherwise explicitly specified.

[0029] The above description is only a preferred embodiment of the present utility model, but the protection scope of the present utility model is not limited thereto. Any equivalent substitutions or changes made by those skilled in the art within the technical scope disclosed in the present utility model, based on the technical solution and the inventive concept of the present utility model, should be included within the protection scope of the present utility model.

Claims

1. A polyacrylamide gel protein recovery device comprising a housing (1) characterised in that: A fixed cylinder (2) is fixedly connected to the inner bottom wall of the box (1). A telescopic cylinder (3) is slidably connected inside the fixed cylinder (2). A screw (4) is threadedly connected inside the telescopic cylinder (3). A servo motor (5) is installed below the box (1). The output end of the servo motor (5) passes through the box (1) and is fixedly connected to the bottom end of the screw (4). Clamping frames (6) are fixedly connected to both sides of the telescopic cylinder (3). Several sets of elastic clips (7) are fixedly connected to the upper surface of the two clamping frames (6). One side of each set of elastic clips (7) is a slope. A dialysis bag is clamped inside one set of elastic clips (7). (8) Two rubber airbags (9) are fixedly connected to the inner walls of the two clamping frames (6). Each rubber airbag (9) is fixedly connected to a pressure relief valve (10) on its upper surface. The air outlet of each pressure relief valve (10) extends through to the top of the clamping frame (6). An air suction pump (11) is fixedly connected to the front of the box (1). An electromagnetic three-way valve (12) is fixedly connected to the output end of the air suction pump (11). A hose (13) is fixedly connected to the second port of the electromagnetic three-way valve (12). A connecting pipe (14) is fixedly connected to the other end of the hose (13). The outer surface of each rubber airbag (9) is fixedly connected to the connecting pipe (14).

2. A polyacrylamide gel protein recovery device according to claim 1, wherein: The bottom surface of the box (1) is fixedly connected with four support legs (16), and electrophoresis power supply (17) is fixedly embedded on both sides of the box (1).

3. The polyacrylamide gel protein recovery device of claim 1, wherein: A rubber sealing ring (18) is fixedly connected to the upper surface of the fixed cylinder (2), and the inner wall of the rubber sealing ring (18) is in contact with the outer surface of the telescopic cylinder (3).

4. The polyacrylamide gel protein recovery device of claim 1, wherein: An anti-detachment ring (19) is fixedly connected to the outer surface of the screw (4), and the upper surface of the anti-detachment ring (19) is in contact with the inner top wall of the telescopic cylinder (3).

5. The polyacrylamide gel protein recovery device of claim 1, wherein: The left side of the servo motor (5) is fixedly connected to a stabilizing base (20), and the upper surface of the stabilizing base (20) is fixedly connected to the bottom surface of the housing (1).

6. The polyacrylamide gel protein recovery device of claim 1, wherein: Both of the clamping frames (6) are fixedly connected to the front and back of the clamping frame (6), and one side of each clamping frame (21) is fixedly connected to the outer surface of the telescopic cylinder (3).

7. The polyacrylamide gel protein recovery device of claim 1, wherein: The bottom surfaces of the two clamping frames (6) are fixedly connected with several limiting plates (22), and the several limiting plates (22) are all inclined at a certain angle. The outer surfaces of the several limiting plates (22) are provided with equidistant ventilation holes (23).

8. The polyacrylamide gel protein recovery device of claim 1, wherein: The air intake end of the air pump (11) is fixedly connected to the air intake hood (24), and the back of the air intake hood (24) is fixedly connected to the front of the box (1).

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