A syringe-integrated surface-enhanced Raman spectroscopy detection platform

Abstract

This invention discloses a syringe-integrated surface-enhanced Raman spectroscopy (SERS) detection platform, belonging to the field of SERS detection technology. The syringe-integrated SERS detection platform includes a syringe barrel; a piston located inside the syringe barrel and slidably connected to it; one end of a SERS needle connected to the piston, capable of moving within the syringe barrel together with the piston; one end of a needle tip connected to the syringe barrel and fitted around the outer circumference of the SERS needle; and the syringe barrel connected to a support. The SERS needle retracts within the syringe barrel and needle tip, which protect the structural integrity of the SERS sensor manufactured by surface deposition, reducing direct friction between the SERS needle and the solid matrix, significantly improving mechanical stability and ease of operation, and avoiding problems such as bending or breakage when the SERS needle is inserted into the solid matrix, as well as the problem of contamination during insertion or removal. Furthermore, the support and base provide stable support for the syringe carrying the SERS needle.

Description

Technical Field

[0001] This utility model relates to a surface-enhanced Raman spectroscopy detection platform integrated with a syringe, belonging to the field of SERS detection equipment technology. Background Technology

[0002] In the field of bioassay, the minimally invasive quantitative detection of trace target analytes in vivo is crucial. Among numerous analytical techniques, surface-enhanced Raman reflectance (SERS) stands out due to its non-destructive potential and ultrasensitive characterization. Currently, most commonly used SERS strategies in vivo involve injecting SERS tags; however, the problem lies in the susceptibility of target information to interference from complex background signals. Existing research primarily obtains SERS signals indirectly from the SERS tags, rather than directly from the target molecule itself. Moreover, the number of SERS tags that can reach the target location in vivo is limited, and this inefficiency severely restricts the application of SERS tags in in vivo detection. Furthermore, most existing portable SERS sensors struggle to directly analyze solid matrices, especially to obtain spectral information from within the sample. Therefore, modern technologies are urgently needed to achieve rapid and efficient direct detection.

[0003] Needle-like substrates with SERS activity offer a promising solution to this challenge. These insertable SERS sensors can be used in applications such as meat quality monitoring, intracellular metabolite tracking, and tumor tissue differentiation detection. Current needle-based designs commonly involve fixing plasma nanoparticles onto optical fibers, using nano-tip techniques, or modifying acupuncture needles. However, these designs suffer from mechanical instability during tissue penetration; SERS needles are prone to bending or breaking during sampling, and are also susceptible to contamination during insertion and withdrawal. Utility Model Content

[0004] This invention provides a surface-enhanced Raman spectroscopy (SERS) detection platform integrated with a syringe, which solves the problems of SERS needles being prone to bending or breaking during tissue penetration, and being easily contaminated during needle insertion or withdrawal.

[0005] This utility model is achieved through the following technical solution:

[0006] This invention provides a syringe-integrated surface-enhanced Raman spectroscopy detection platform, including...

[0007] Syringe;

[0008] The piston is located inside the syringe and is slidably connected to the syringe.

[0009] The SERS needle is connected to the piston at one end and can move together with the piston inside the syringe.

[0010] A needle, one end of which is connected to the syringe and fitted around the outer periphery of the SERS needle;

[0011] A support, wherein the syringe is connected to the support.

[0012] In one embodiment of this invention, the SERS needle and the piston are detachably connected, facilitating the replacement of the SERS needle.

[0013] In one embodiment of this invention, the length of the SERS needle is less than the sum of the lengths of the syringe barrel and the needle tip. This ensures that the SERS needle can be completely retracted into the syringe barrel and needle tip when retracted, thus protecting the SERS needle and preventing it from bending or breaking during insertion into the solid matrix. Simultaneously, it also ensures that the SERS needle is not contaminated during insertion or withdrawal.

[0014] In one embodiment of this utility model, the needle is a hollow round tube, and the diameter of the needle tube is larger than the diameter of the SERS needle.

[0015] In one embodiment of this utility model, a push rod is included, which is connected to the piston.

[0016] In one embodiment of this utility model, the bracket includes a connector and a fixing member, and the connector is connected to the fixing member.

[0017] In one embodiment of this invention, the fixing member is a cylindrical structure with an opening, and the fixing member is interference-fitted with the syringe. This ensures the stability of the syringe during the detection process and prevents shaking. This enables portable SERS detection and can be applied to various scenarios.

[0018] In one embodiment of this utility model, a base is included, and the connecting member is connected to the base.

[0019] In one embodiment of this invention, the lengths of the SERS needle and the needle tip are adapted to the sample depth.

[0020] In one embodiment of this invention, the needle is made of stainless steel. This protects the SERS needle during use, preventing structural damage and improving measurement accuracy.

[0021] Beneficial effects

[0022] This invention provides a syringe-integrated surface-enhanced Raman spectroscopy (SERS) detection platform, comprising a syringe barrel, piston, needle, SERS needle, support, and base. By combining the SERS needle with the syringe and equipping it with the support and base, a SERS detection platform is formed. The SERS needle retracts within the syringe barrel and needle, which protect the structural integrity of the SERS sensor manufactured by surface deposition, reducing direct friction between the SERS needle and the solid matrix. This significantly improves mechanical stability and ease of operation, avoiding bending or breakage of the SERS needle during insertion into the solid matrix and preventing contamination during insertion or removal. Furthermore, the support and base provide stable support for the syringe carrying the SERS needle, enabling rapid on-site SERS detection and portable Raman probe data acquisition. Attached Figure Description

[0023] Figure 1 The front view of the surface-enhanced Raman spectroscopy detection platform provided by this utility model.

[0024] Figure 2 A cross-sectional view of the SERS needle sampling state provided by this utility model.

[0025] Figure 3 A perspective view of the base and bracket provided for this utility model.

[0026] In the picture:

[0027] 1. Syringe; 2. Push rod; 3. Piston; 4. Needle; 5. SERS needle; 6. Support; 61. Connector; 62. Fixing element; 7. Base; 8. Solid matrix. Detailed Implementation

[0028] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those skilled in the art without creative effort are within the protection scope of the present utility model.

[0029] In this utility model, unless otherwise explicitly specified and limited, the terms "connected," "linked," and "fixed" should be interpreted broadly. For example, they can refer to a fixed connection, a detachable connection, or an integral part; they can refer to a mechanical connection or an electrical connection; they can refer to a direct connection or an indirect connection through an intermediate medium; they can refer to the internal communication of two components or the interaction between two components. Those skilled in the art can understand the specific meaning of the above terms in this utility model based on the specific circumstances.

[0030] In this invention, unless otherwise explicitly specified and limited, "above" or "below" the second feature can include direct contact between the first and second features, or contact between the first and second features through another feature between them. Furthermore, "above," "over," and "on top" of the second feature includes the first feature directly above or diagonally above the second feature, or simply indicates that the first feature is at a higher horizontal level than the second feature. "Below," "below," and "under" the second feature includes the first feature directly below or diagonally below the second feature, or simply indicates that the first feature is at a lower horizontal level than the second feature.

[0031] like Figures 1 to 3 As shown, this application provides a syringe-integrated surface-enhanced Raman spectroscopy (SERS) detection platform. This platform can acquire data in scenarios such as meat quality testing, intracellular metabolite tracking, and tumor tissue differentiation detection, while maintaining mechanical stability and preventing bending or breakage during acquisition. It also avoids the problem of contamination during SERS needle insertion or withdrawal. The syringe-integrated SERS detection platform includes a syringe barrel 1, a push rod 2, a piston 3, a needle 4, an SERS needle 5, a support 6, and a base 7. The syringe barrel 1 is a cylinder with an internal cavity. The piston 3 is installed inside the syringe barrel 1, and one end of the push rod 2 is connected to the piston 3. Pushing or pulling the push rod 2 drives the piston 3 to reciprocate within the syringe barrel 1. The needle 4 is a hollow cylindrical tube, and one end of the needle 4 is connected to the side of the syringe barrel 1 opposite to the push rod 2. One end of the SERS needle 5 is connected to the end of the piston 3 opposite to the push rod 2 and can reciprocate along with the piston 3. The SERS needle 5 passes through the needle tip 4. The length of the SERS needle 5 should be less than the sum of the length of the syringe barrel 1 and the length of the needle tip 4. This ensures that when the SERS needle retracts, it can be completely retracted into the syringe barrel 1 and the needle tip 4, thus protecting the SERS needle 5 and preventing it from bending or breaking during insertion into the solid matrix 8. At the same time, it also ensures that the SERS needle will not be contaminated during insertion or withdrawal.

[0032] In some embodiments, the base 7 is a plate-like structure, and the bracket 6 is mounted on the base 7. The bracket 6 is used to mount the syringe 1, providing stable support for the detection of SERS needle samples and preventing it from rolling during detection. This enables portable detection. The bracket 6 includes a connector 61 and a fixing member 62. The connector 61 is connected to the base 7 and the fixing member 62 on both sides, respectively. The fixing member 62 is a cylindrical structure with an opening. The inner diameter of the fixing member 62 is smaller than the outer diameter of the syringe 1. The syringe 1 is inserted into the fixing member 62 through the opening. The fixing member 62 and the syringe 1 are interference-fitted to ensure the stability of the syringe 1 during the detection process and prevent shaking. This enables portable SERS detection and can be applied to various different scenarios.

[0033] Specifically, in this embodiment, due to the limitation of syringe depth, the length of the SERS needle 5 used in this application is approximately 10.5 cm. Since the length of the needle tip 4 is limited, its insertion depth into the solid matrix 8 is also limited. Therefore, the insertion depth of the SERS needle 5 into the solid matrix 8 is the same as the insertion depth of the needle tip 4 into the solid matrix 8. For solid matrix 8 at different depths in the syringe, the lengths of the SERS needle 5 and the needle tip 4 can be adapted to the depth of the solid matrix 8.

[0034] Furthermore, in some embodiments, the SERS needle 5 is a silver needle with nanomaterials loaded on its surface. When the SERS needle penetrates the solid matrix 8, the nanomaterials loaded on the surface of the SERS needle will be wiped off, causing detection errors. This application can retract the SERS needle 5 into the syringe 1 and needle 4 by moving the push rod 2 to protect the SERS needle 5 from structural damage during sampling, thereby improving the accuracy of the measurement.

[0035] Optionally, the piston 3 and the SERS needle 5 are detachably connected to facilitate the replacement of the SERS needle 5.

[0036] Optionally, piston 3 is made of rubber. Needle 4 is made of stainless steel.

[0037] The working principle of this invention is as follows: One end of the SERS needle 5 is fixedly connected to the piston 3. The SERS needle is pulled into the syringe barrel 1 and needle tip 4 for protection by pulling the push rod 2. Then, the end of the syringe with the needle tip 4 is inserted into the solid matrix 8. Pushing the push rod 2 pulls out the needle tip 4, allowing the SERS needle 5 to contact the solid matrix 8, exposing the active sensing surface of the SERS needle 5 for direct molecular interaction and enrichment. After a sufficient adsorption period, the push rod 2 pushes the needle tip 4 back to its original position, covering the SERS needle 5. It is then withdrawn from the solid matrix 8, rinsed, and fixed in the fixing member 62. Pushing the push rod 2 exposes the SERS needle 5, after sample collection, to the outside of the needle tip 4. A portable spectrometer is used to scan the SERS needle 5, enabling the creation of a spatial map of the analyte. Combining the SERS needle 5 with the syringe protects the structural integrity of the SERS sensor manufactured by surface deposition, reduces direct friction between the SERS needle 5 and the solid matrix, and significantly improves mechanical stability and ease of operation. The bracket 6 and base 7 provide a stable support for the syringe carrying the SERS needle 5, enabling the SERS platform to perform rapid on-site SERS testing and realize portable Raman probe data acquisition.

[0038] The technical features of the above embodiments can be combined in any way. For the sake of brevity, not all possible combinations of the technical features in the above embodiments are described. However, as long as there is no contradiction in the combination of these technical features, they should be considered to be within the scope of this specification.

[0039] The above embodiments only illustrate several implementation methods of this utility model, and their descriptions are relatively specific and detailed, but they should not be construed as limiting the scope of the invention patent. It should be noted that those skilled in the art can make several modifications and improvements without departing from the concept of this utility model, and these all fall within the protection scope of this utility model.

[0040] This document uses specific embodiments to illustrate the principles and implementation methods of this utility model. The descriptions of these embodiments are merely for the purpose of helping to understand the method and core ideas of this utility model. It should be noted that those skilled in the art can make various improvements and modifications to this utility model without departing from its principles, and these improvements and modifications also fall within the protection scope of the claims of this utility model. Therefore, the protection scope of this utility model patent should be determined by the appended claims.

Claims

1. A syringe integrated surface enhanced Raman spectroscopy detection platform, characterized in that, include: Syringe (1); The piston (3) is located inside the syringe (1) and is slidably connected to the syringe (1); The SERS needle (5) is connected at one end to the piston (3) and is slidably connected to the syringe (1) through the piston (3); A needle (4), one end of which is connected to the syringe (1) and sleeved on the outer periphery of the SERS needle (5); The support (6) is connected to the syringe (1).

2. The syringe integrated surface enhanced Raman spectroscopy detection platform according to claim 1, wherein, The SERS needle (5) and the piston (3) are detachably connected.

3. The syringe integrated surface enhanced Raman spectroscopy detection platform according to claim 2, wherein, The length of the SERS needle (5) is less than the sum of the lengths of the syringe (1) and the needle (4).

4. The syringe integrated surface enhanced Raman spectroscopy detection platform according to claim 3, wherein, The needle (4) is a hollow round tube, and the diameter of the needle (4) is larger than the diameter of the SERS needle (5).

5. The syringe integrated surface enhanced Raman spectroscopy detection platform according to claim 1, wherein, It includes a push rod (2), which is connected to the piston (3).

6. The syringe integrated surface enhanced Raman spectroscopy detection platform according to claim 1, wherein, The bracket (6) includes a connector (61) and a fixing member (62), wherein the connector (61) is connected to the fixing member (62).

7. The syringe integrated surface enhanced Raman spectroscopy detection platform according to claim 6, wherein, The fixing member (62) is a cylindrical structure with an opening, and the fixing member (62) is interference-fitted with the syringe (1).

8. The syringe integrated surface enhanced Raman spectroscopy detection platform according to claim 6, wherein, Includes a base (7), and the connector (61) is connected to the base (7).

9. The syringe integrated surface enhanced Raman spectroscopy detection platform according to claim 4, wherein, The lengths of the SERS needle (5) and the needle tip (4) are adapted to the sample depth.

10. A syringe-integrated surface-enhanced Raman spectroscopy detection platform according to any one of claims 1-9, characterized in that, The needle (4) is made of stainless steel.

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