Infusion fixture
By designing a perfusion fixation device that uses a diversion container and pump to maintain a constant fixative level, the problem of pressure fluctuations during lung perfusion in medium and large animals was solved, ensuring normal alveolar expansion and tissue morphology stability, and improving the accuracy of pathological diagnosis.
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- GUANGZHOU NAT LAB
- Filing Date
- 2025-09-01
- Publication Date
- 2026-07-21
Smart Images
Figure CN224522209U_ABST
Abstract
Description
Technical Field
[0001] This application relates to the field of injection and fixation technology, and in particular to an injection and fixation device. Background Technology
[0002] Tissue fixation stabilizes intracellular proteins, nucleic acids, and other components, preventing autolysis, and is a crucial initial step in pathological examination. The lungs consist of alveoli, bronchi, blood vessels, and interstitium; the alveolar walls are thin and contain numerous cavities. An inflated lung is composed of 90% air and 10% tissue. After removal from the body, due to pressure changes, internal gas is expelled, and the alveoli compress due to lack of support. For organs like the lungs, which are complex and easily damaged, the method and quality of tissue fixation directly affect the accuracy of subsequent morphological observation and pathological diagnosis.
[0003] Methods for maintaining lung expansion include endotracheal instillation fixation, in situ fixation, fixed volume fixation, and vacuum expansion. In endotracheal instillation fixation, in particular, maintains normal lung morphology and minimizes changes in tissue structure, making it especially suitable for research on emphysema or diffuse lung disease. Rapid operation and constant pressure are crucial during lung instillation fixation. Small laboratory animals like mice have relatively small lungs, allowing for rapid endotracheal instillation. However, for medium to large animals, the required fixative volume is large, the process is time-consuming, and pressure fluctuations directly affect the instillation outcome. Insufficient pressure leads to incomplete alveolar expansion and poor lung morphology, while excessive pressure can cause alveolar damage. Currently, controlling instillation pressure through manual observation and adjustment is time-consuming, labor-intensive, and prone to errors, making it difficult to maintain constant pressure throughout the instillation process. Utility Model Content
[0004] Therefore, it is necessary to provide an infusion fixation device to address the above problems. This device can set the required pressure value before infusion and automatically maintain a constant pressure during infusion to prevent organ and tissue damage and morphological abnormalities caused by pressure changes during infusion.
[0005] This application provides an injection fixing device, comprising:
[0006] Mounting rack;
[0007] A storage container for storing a fixative;
[0008] The diversion container is height-adjustably mounted on a mounting frame. The diversion container has an outlet and an overflow, and the height of the overflow is greater than the height of the outlet.
[0009] A replenishment pipeline is provided, which connects the storage container and the distribution container. A pump body is provided on the replenishment pipeline, which is used to pump the fixative in the storage container to the distribution container.
[0010] A valve body, which is connected to the liquid outlet, is used to open or close the liquid outlet;
[0011] The outlet pipe has one end connected to the outlet of the valve body and the other end connected to the organ to be infused.
[0012] The technical solution will be further explained below:
[0013] In one embodiment, the infusion fixing device further includes a recovery pipeline, one end of which is connected to the overflow port and the other end of which is connected to the storage container.
[0014] In one embodiment, the mounting bracket includes:
[0015] Base
[0016] A column, which is fixed to the base;
[0017] A support frame is movably connected to the column and to the diversion container, and the support frame can move up and down along the column;
[0018] A locking element, which is connected to the bracket and used to lock the bracket onto the column.
[0019] In one embodiment, the column is provided with scale lines.
[0020] In one embodiment, the top of the storage container has an injection port for adding a fixative, and the injection port is sealed with a first sealing cap.
[0021] In one embodiment, the replenishment line is connected to the bottom of the storage container.
[0022] In one embodiment, the infusion fixing device further includes an air inlet pipe, one end of which extends into the diversion container and the other end of which extends out of the diversion container, and the height of the end of the air inlet pipe extending into the diversion container is greater than the height of the overflow port.
[0023] In one embodiment, the top of the diversion container is sealed with a second sealing cap, and both the air inlet pipe and the liquid replenishment pipe extend through the second sealing cap into the diversion container; and / or,
[0024] The outlet is located at the bottom of the diversion container; and / or,
[0025] The overflow port is located on the side wall of the diversion container.
[0026] In one embodiment, the valve body is connected to a multi-port pipe, which has multiple outlets, each of which is connected to a liquid outlet pipe.
[0027] In one embodiment, the perfusion fixation device further includes an organ container for accommodating the organ to be perfused.
[0028] In the aforementioned perfusion fixation device, since the perfusion pressure is only related to the level of the fixative in the distribution container, the level of the fixative in the distribution container can be adjusted to the required experimental level by adjusting the height of the distribution container on the mounting frame before perfusion, thus achieving the desired perfusion pressure value. Simultaneously, during perfusion, the pump continuously introduces the fixative from the storage container into the distribution container, and excess fixative flows out from the overflow port of the distribution container, maintaining a stable level of the fixative in the distribution container. This ensures a constant perfusion pressure during perfusion, preventing organ and tissue damage and morphological abnormalities caused by pressure fluctuations. Therefore, it is suitable not only for organ perfusion fixation in small laboratory animals such as mice but also for medium to large animals. Furthermore, the perfusion pressure is automatically maintained at a constant level during perfusion, avoiding the uncertainty of manual pressure control and reducing the operator's workload. Attached Figure Description
[0029] The accompanying drawings, which form part of this application, are used to provide a further understanding of this application. The illustrative embodiments of this application and their descriptions are used to explain this application and do not constitute an undue limitation of this application.
[0030] To more clearly illustrate the technical solutions in the embodiments of this application, the accompanying drawings used in the description of the embodiments will be briefly introduced below. Obviously, the accompanying drawings described below are only some embodiments of this application. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort.
[0031] Furthermore, the accompanying drawings are not drawn to a 1:1 scale, and the relative dimensions of the various components are shown as examples only and not necessarily to scale. In the accompanying drawings:
[0032] Figure 1 This is a schematic diagram of the structure of an injection fixing device according to an embodiment.
[0033] Figure 2This is a schematic diagram of the diversion container and mounting frame of the infusion fixing device shown in Figure 1.
[0034] Explanation of reference numerals in the attached figures:
[0035] 10. Mounting bracket; 11. Base; 12. Column; 13. Bracket; 14. Locking element; 15. Scale line; 20. Storage container; 21. Injection port; 22. First sealing cap; 30. Diversion container; 31. Overflow port; 32. Outlet port; 33. Valve body; 34. Second sealing cap; 40. Replenishment pipeline; 41. Pump body; 50. Outlet pipeline; 60. Recovery pipeline; 70. Air inlet pipe; 80. Organ container. Detailed Implementation
[0036] To make the above-mentioned objectives, features, and advantages of this application more apparent and understandable, the specific embodiments of this application are described in detail below with reference to the accompanying drawings. Many specific details are set forth in the following description to provide a thorough understanding of this application. However, this application can be implemented in many other ways different from those described herein, and those skilled in the art can make similar modifications without departing from the spirit of this application. Therefore, this application is not limited to the specific embodiments disclosed below.
[0037] In the description of this application, it should be understood that if terms such as "center", "longitudinal", "lateral", "length", "width", "thickness", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", "clockwise", "counterclockwise", "axial", "radial", "circumferential" appear, these terms indicate the orientation or positional relationship based on the orientation or positional relationship shown in the accompanying drawings, and are only for the convenience of describing this application and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation, and therefore should not be construed as a limitation of this application.
[0038] Furthermore, where the terms "first" and "second" appear, these terms are for descriptive purposes only and should not be construed as indicating or implying relative importance or implicitly specifying the number of technical features indicated. Thus, a feature defined with "first" or "second" may explicitly or implicitly include at least one of that feature. In the description of this application, where the term "multiple" appears, "multiple" means at least two, such as two, three, etc., unless otherwise explicitly specified.
[0039] In this application, unless otherwise expressly specified and limited, the terms "installation," "connection," "joining," and "fixing," etc., should be interpreted broadly. For example, they can refer to a fixed connection, a detachable connection, or an integral part; they can refer to a mechanical connection or an electrical connection; they can refer to a direct connection or an indirect connection through an intermediate medium; they can refer to the internal communication of two components or the interaction between two components, unless otherwise expressly limited. Those skilled in the art can understand the specific meaning of the above terms in this application based on the specific circumstances.
[0040] In this application, unless otherwise expressly specified and limited, the use of descriptions such as "above" or "below" the second feature indicates that the first and second features are in direct contact or indirect contact via an intermediate medium. Furthermore, "above," "on top of," and "over" the second feature can mean that the first feature is directly above or diagonally above the second feature, or simply that the first feature is at a higher horizontal level than the second feature. Similarly, "below," "below," and "under" the second feature can mean that the first feature is directly below or diagonally below the second feature, or simply that the first feature is at a lower horizontal level than the second feature.
[0041] It should be noted that if an element is referred to as being "fixed to" or "set on" another element, it can be directly on the other element or there may be an intervening element. If an element is considered to be "connected to" another element, it can be directly connected to the other element or there may be an intervening element. If so, the terms "vertical," "horizontal," "upper," "lower," "left," "right," and similar expressions used in this application are for illustrative purposes only and do not represent the only possible implementation.
[0042] See Figure 1 , Figure 1 A schematic diagram of the infusion and fixing device according to one embodiment of this application is shown. Specifically, the infusion and fixing device of one embodiment includes a mounting frame 10, a storage container 20, a diversion container 30, a replenishment pipeline 40, an outlet pipeline 50, and a valve body 33. Wherein:
[0043] Storage container 20 is used to store a fixative. Specifically, the fixative includes, but is not limited to, paraformaldehyde or formalin.
[0044] The diversion container 30 is height-adjustable on the mounting bracket 10, meaning the height of the diversion container 30 on the mounting bracket 10 is adjustable. Furthermore, the diversion container 30 is provided with an outlet 32 and an overflow outlet 31, and the height of the overflow outlet 31 is greater than the height of the outlet 32.
[0045] The replenishment line 40 connects the storage container 20 and the diversion container 30. A pump body 41 is provided on the replenishment line 40. The pump body 41 is used to pump the fixative in the storage container 20 to the diversion container 30. For example, the pump body 41 can be a peristaltic pump or the like, and there is no limitation here.
[0046] The valve body 33 is connected to the liquid outlet 32. The valve body 33 is used to open or close the liquid outlet 32. For example, the valve body 33 can be an electrically controlled solenoid valve or a manually controlled control valve, etc., without limitation.
[0047] One end of the outlet pipe 50 is connected to the outlet of the valve body 33, and the other end of the outlet pipe 50 is used to connect to the organ to be perfused. Specifically, the organ to be perfused can be the lungs, heart, liver, etc., without limitation.
[0048] For example, taking lung perfusion fixation as an example, before operation, the above-mentioned perfusion fixation device first injects the required fixative into the storage container 20 and keeps the valve 33 closed. Then, the pump 41 is turned on, so that the pump 41 pumps the fixative in the storage container 20 to the distribution container 30. When the liquid level in the distribution container 30 reaches the overflow port 31, due to gravity and negative pressure, the excess fixative will flow out through the overflow port 31, so that the liquid level in the distribution container 30 remains constant to ensure stable perfusion pressure. After the liquid level in the distribution container 30 is stable, the height of the distribution container 30 on the mounting frame 10 is adjusted so that the liquid level meets the experimental requirements. Finally, the outlet tube 50 is inserted into the airway of the lung, the valve 33 is opened, and the fixative flows slowly into the airway along the outlet tube 50. After confirming that the outlet tube 50 is not blocked and that the lung or lung lobe can be normally filled, the airway opening is tied to ensure that the outlet tube 50 is tightly connected to the airway and there is no air leakage. Keep the pump body 41 running continuously so that the fixative can continuously enter the lungs or lung lobes, ultimately achieving perfusion fixation of the lungs.
[0049] In the aforementioned perfusion fixation device, since the perfusion pressure is only related to the liquid level of the fixative in the distribution container 30, the liquid level of the fixative in the distribution container 30 can be adjusted to the required experimental height by adjusting the height of the distribution container 30 on the mounting frame 10 before perfusion, thereby achieving the setting of the required perfusion pressure value. Simultaneously, during perfusion, the pump body 41 continuously introduces the fixative from the storage container 20 into the distribution container 30, and excess fixative can flow out from the overflow port 31 of the distribution container 30, thus maintaining the liquid level of the fixative in the distribution container 30 at a stable height. This ensures a constant perfusion pressure during perfusion, preventing organ and tissue damage and morphological abnormalities caused by changes in perfusion pressure. Therefore, it is suitable not only for organ perfusion fixation in small experimental animals such as mice but also for organ perfusion fixation in medium and large animals. Furthermore, the perfusion pressure can be automatically maintained at a constant state during perfusion, avoiding the uncertainty caused by manual control of the perfusion pressure and reducing the operator's workload.
[0050] Optionally, in some embodiments, the infusion fixation device further includes a recovery pipeline 60, one end of which is connected to the overflow port 31 and the other end of which is connected to the storage container 20. In this way, after the excess fixative flows out of the overflow port 31 of the diversion container 30, it can flow back to the storage container 20 along the recovery pipeline 60, realizing the recycling of the overflowed fixative and avoiding material waste.
[0051] Optionally, in some embodiments, the height of the end of the recovery pipe 60 connected to the overflow port 31 is greater than the height of the end of the recovery pipe 60 connected to the storage container 20. This ensures that the stationary liquid flowing out of the overflow port 31 of the diversion container 30 can automatically flow back to the storage container 20 along the recovery pipe 60 under the action of gravity, eliminating the need to install a power source on the recovery pipe 60, thereby saving costs.
[0052] It is worth mentioning that, in another embodiment, the height of the end of the recovery pipe 60 connected to the overflow port 31 can also be less than or equal to the height of the end of the recovery pipe 60 connected to the storage container 20. In this case, the negative pressure generated in the storage container 20 after the pump body 41 is turned on can also be used to automatically return the overflowing fixative liquid to the storage container 20 along the recovery pipe 60.
[0053] Understandably, in other embodiments, excess fixative can be diverted to other containers or devices after flowing out of the overflow port 31 of the diversion container 30, as long as the capacity of the fixative in the storage container 20 is sufficient to meet the group filling requirements, and no restrictions are imposed here.
[0054] See Figure 2In some embodiments, the mounting frame 10 includes a base 11, a column 12, a bracket 13, and a locking member 14. The column 12 is fixed to the base 11 so that it can be vertically fixed on the working platform. The bracket 13 is movably connected to the column 12 and connected to the diversion container 30. The bracket 13 can move up and down along the column 12. The locking member 14 is connected to the bracket 13 and used to lock the bracket 13 to the column 12. Thus, by first installing the diversion container on the bracket 13, then moving the bracket 13 on the column 12 to the required height, and finally locking the bracket 13 to the column 12 by the locking member 14, the height of the diversion container 30 can be adjusted to set the injection pressure to the required value before injection.
[0055] Specifically, in one embodiment, the bracket 13 is clamped or held onto the diversion container 30 to fix the diversion container 30. At the same time, the bracket 13 can be slidably sleeved on the column 12, and the locking member 14 can be a threaded knob, which passes through the bracket 13 and abuts against the column 12. By tightening the knob, the bracket 13 can be locked onto the column 12. Understandably, in other embodiments, the locking member 14 can also be a buckle or the like, which locks the bracket 13 onto the column 12 by snapping.
[0056] See Figure 2 In some embodiments, the column 12 is provided with scale lines 15. Specifically, the scale lines 15 can provide real-time feedback on the height of the bracket 13 on the column 12, making it convenient to adjust the height of the bracket 13 on the column 12.
[0057] See Figure 1 In one embodiment, the top of the storage container 20 is provided with an injection port 21 for adding fixative, and the injection port 21 is sealed with a first sealing cap 22. Thus, before filling, the fixative can be injected into the storage container 20 through the injection port 21, and then the injection port 21 can be sealed by the first sealing cap 22 to prevent the fixative from spilling or evaporating from the injection port 21. After the pump body 41 is turned on, the storage container 20 is kept under negative pressure, thereby ensuring that the fixative flowing out of the overflow port 31 of the diversion container 30 can continuously flow back to the storage container 20 along the recovery pipeline 60.
[0058] See Figure 1 In some embodiments, the replenishment line 40 is connected to the bottom of the storage container 20. This allows for full utilization of the fixative in the storage container 20, prevents residual fixative at the bottom of the storage container 20 from being unable to be drawn out by the pump body 41, and avoids material waste.
[0059] Optionally, in some embodiments, the top of the diversion container 30 is sealed with a second sealing cap 34. By sealing the top opening of the diversion container 30 with the second sealing cap 34, the fixative liquid inside the diversion container 30 can be prevented from spilling out of the top opening and the evaporation of the fixative liquid can be reduced.
[0060] When a second sealing cap 34 is provided on the top of the diversion container 30, see [reference needed]. Figure 1 The filling and fixing device also includes an air inlet pipe 70. One end of the air inlet pipe 70 extends into the distribution container 30, and the other end extends out of the distribution container 30. The height of the end of the air inlet pipe 70 extending into the distribution container 30 is greater than the height of the overflow port 31. In this way, the air inlet pipe 70 connects the inside and outside of the distribution container 30, ensuring that the air pressure above the liquid surface inside the distribution container 30 is consistent with the external atmospheric pressure. The filling pressure is only related to the liquid level of the fixing liquid in the distribution container 30, and it avoids the problem of the fixing liquid not being able to flow out from the outlet 32 due to excessively low air pressure inside the distribution container 30.
[0061] Optionally, in some embodiments, the air inlet pipe 70 and the liquid replenishment pipe 40 both extend through the second sealing cover 34 into the diversion container 30. The second sealing cover 34 can fix the air inlet pipe 70 and the liquid replenishment pipe 40, ensuring stable communication between the air inlet pipe 70 and the liquid replenishment pipe 40 and the diversion container 30.
[0062] See Figure 1 In one embodiment, the outlet 32 is located at the bottom of the diversion container 30, thus ensuring that the fixative in the diversion container 30 can be fully discharged through the outlet 32, avoiding the presence of residual fixative in the diversion container 30.
[0063] See Figure 1 In some embodiments, the overflow port 31 is located on the side wall of the diversion container 30. This ensures that the height of the overflow port 31 is higher than the height of the liquid outlet 32.
[0064] Optionally, in some embodiments, the valve body 33 is connected to a multi-port tube (not shown), which has multiple outlets, each of which is connected to a liquid outlet line 50. Specifically, different liquid outlet lines 50 are used to connect to different organs to be perfused, such as different lungs or different lobes. This allows for simultaneous constant-pressure perfusion of multiple organs, improving perfusion efficiency.
[0065] See Figure 1 In one embodiment, the perfusion fixation device further includes an organ container 80, which is used to hold the organ to be perfused, thereby preventing damage or contamination of the organ during the perfusion process. At the same time, the organ container 80 can also receive fixative solution that is accidentally leaked from the organ or the outlet tube 50 during the perfusion process, preventing the leaked fixative solution from polluting the environment.
[0066] The technical features of the above embodiments can be combined in any way. For the sake of brevity, not all possible combinations of the technical features in the above embodiments are described. However, as long as there is no contradiction in the combination of these technical features, they should be considered to be within the scope of this specification.
[0067] The embodiments described above are merely illustrative of several implementation methods of this application, and while the descriptions are relatively specific and detailed, they should not be construed as limiting the scope of the patent application. It should be noted that those skilled in the art can make various modifications and improvements without departing from the concept of this application, and these all fall within the protection scope of this application. Therefore, the protection scope of this patent application should be determined by the appended claims.
Claims
1. A grouting fixing device, characterized in that, include: Mounting bracket (10); Storage container (20) for storing fixative; Diversion container (30), the diversion container (30) is height-adjustably mounted on the mounting bracket (10), the diversion container (30) is provided with a liquid outlet (32) and an overflow port (31), and the height of the overflow port (31) is greater than the height of the liquid outlet (32); A replenishment pipeline (40) is provided, which connects the storage container (20) and the diversion container (30). A pump body (41) is provided on the replenishment pipeline (40), which is used to pump the solid liquid in the storage container (20) to the diversion container (30). Valve body (33), which is connected to the liquid outlet (32), and the valve body (33) is used to open or close the liquid outlet (32). The outlet pipe (50) is connected at one end to the outlet of the valve body (33) and at the other end to the organ to be perfused.
2. The injection fixing device according to claim 1, characterized in that, The infusion fixing device also includes a recovery pipeline (60), one end of which is connected to the overflow port (31), and the other end of which is connected to the storage container (20).
3. The injection fixing device according to claim 1, characterized in that, The mounting bracket (10) includes: Base (11); A column (12) is fixed to the base (11); A support (13) is movably connected to the column (12) and connected to the diversion container (30). The support (13) can move up and down along the column (12). Locking element (14), which is connected to the bracket (13) and used to lock the bracket (13) onto the column (12).
4. The injection fixing device according to claim 3, characterized in that, The column (12) is provided with scale lines (15).
5. The injection fixing device according to claim 1, characterized in that, The top of the storage container (20) is provided with an injection port (21) for adding fixative, and the injection port (21) is sealed with a first sealing cap (22).
6. The injection fixing device according to claim 1, characterized in that, The replenishment line (40) is connected to the bottom of the storage container (20).
7. The injection fixing device according to claim 1, characterized in that, The filling and fixing device also includes an air inlet pipe (70), one end of which extends into the diversion container (30), the other end of which extends out of the diversion container (30), and the height of the end of the air inlet pipe (70) extending into the diversion container (30) is greater than the height of the overflow port (31).
8. The injection fixing device according to claim 7, characterized in that, The top of the diversion container (30) is sealed with a second sealing cap (34), and the air inlet pipe (70) and the liquid replenishment pipe (40) both extend through the second sealing cap (34) into the diversion container (30); and / or, The outlet (32) is located at the bottom of the diversion container (30); and / or, The overflow port (31) is located on the side wall of the diversion container (30).
9. The injection fixing device according to claim 1, characterized in that, The valve body (33) is connected to a multi-port pipe, which has multiple outlets, and each outlet is connected to a liquid outlet pipe (50).
10. The injection fixing device according to any one of claims 1-9, characterized in that, The perfusion fixation device also includes an organ container (80) for holding the organ to be perfused.