An apparatus for ex vivo storage and culture of living organs

Abstract

The utility model discloses a kind of in-vivo organ in-vitro storage culture devices, belong to organ culture technical field, it includes refrigeration storage box, the middle part in the refrigeration storage box is fixedly connected with support frame, the through hole being set in the top four corners of support frame is fixedly connected with storage cylinder in penetration. By organ container extrusion booster assembly to make its internal gas import airway, make four slide plates respectively through multiple slide column drive four clamping plates synchronous mutual approach and with the outer wall of organ container, organ container is driven to insert into slot, four clamping plates are in locking state and organ container is clamped, improve the stability of the culture device work, since the movable range of clamping plate is controlled according to the force of organ container depression, and the height of each layer slot is different, and the current position of clamping plate can be locked by inserting different height slot, so as to limit different specifications organ container, make the compatibility of the device strong.

Description

Technical Field

[0001] This invention belongs to the field of organ culture technology, specifically a device for in vitro storage and culture of living organs. Background Technology

[0002] In the field of modern medicine, organ transplantation is an important means of saving the lives of patients with end-stage organ failure. If living organs (such as kidneys, livers, and hearts) are not properly stored and cared for after being removed from the body, they will suffer from tissue damage and loss of function due to ischemia, hypoxia, and metabolic disorders, which will greatly reduce the success rate of transplantation.

[0003] Currently, in the in vitro storage and culture of living organs, the container containing the living organ is placed in a refrigerated storage box, and a dynamic perfusion culture system is used to simulate in vivo hemodynamics and provide the organ with continuous oxygenated nutrient solution perfusion.

[0004] In the in vitro storage and culture technology of living organs, the stable fixation of organ containers is a fundamental condition for ensuring the consistency of the culture environment and avoiding mechanical damage. Since refrigerated storage boxes are only suitable for containers of a single size, the entire clamp needs to be replaced when changing to containers of different sizes, resulting in poor compatibility. Moreover, rigid compression can easily cause thin-walled containers to deform or even be damaged. Therefore, there are significant limitations in the fixation solutions for containers of different sizes. Utility Model Content

[0005] To overcome the above-mentioned defects, this utility model provides a live organ in vitro storage and culture device, which solves the problems that the refrigerated storage box is only suitable for a single-size container, and the clamps need to be replaced as a whole when changing to different sizes of containers, resulting in poor compatibility, and that rigid compression can easily cause deformation or even damage to thin-walled containers.

[0006] To achieve the above objectives, this utility model provides the following technical solution: an in vitro storage and culture device for living organs, comprising a refrigerated storage box, a support frame fixedly connected to the middle of the refrigerated storage box, a storage cylinder fixedly connected through through holes at the four corners of the top of the support frame, an organ container installed inside the storage cylinder, an infusion pipeline connected to the top of the organ container, two infusion pipelines on the same side connected to the other ends of an infusion culture monitoring system, two infusion culture monitoring systems respectively installed on both sides inside the refrigerated storage box, four limiting components attached to the outside of the organ container, each limiting component including a clamping plate, several sliding columns fixed to the outside of the clamping plate, an air passage opened in the protruding part inside the storage cylinder, the other ends of the several sliding columns passing through the air passage and fixed with a sliding plate, the sliding plate slidably connected inside the air passage, a pressurizing component attached to the bottom of the organ container, the four ends of the pressurizing component passing through the storage cylinder and communicating with the four air passages.

[0007] As a further embodiment of this utility model: a lid is hinged to one side of the refrigerated storage box, a control panel is installed on the front of the refrigerated storage box, and handles are provided on both sides of the refrigerated storage box.

[0008] As a further embodiment of this utility model: four inserts are fixed on the upper part of the outer wall of the organ container, the inserts are provided with anti-slip texture, and several slots are opened in the four protruding parts at the top of the storage cylinder, and the inserts are inserted into one of the slots.

[0009] As a further embodiment of this utility model: the clamping plate is arc-shaped and has anti-slip texture on the inner wall, and the bottom ends of the four clamping plates are located above the pressurization component.

[0010] As a further embodiment of this utility model: the pressurizing component includes a pressure seat, the top of which is an arc-shaped design and overlaps with the bottom of the organ container, a piston plate is fixedly connected to the bottom of the pressure seat, a fixed cylinder slides outside the piston plate, and the fixed cylinder is fixed to the bottom of the support frame.

[0011] As a further embodiment of this utility model: a spring is fixedly connected to the bottom of the inner wall of the fixed cylinder, the top end of the spring is fixed to the bottom of the piston plate, and four air guide pipes are connected to the outside of the fixed cylinder, the top end of the air guide pipes penetrates the storage cylinder and communicates with the air passage.

[0012] Compared with the prior art, the beneficial effects of this utility model are as follows:

[0013] 1. This in vitro storage and culture device for living organs involves inserting the bottom of an organ container into a storage cylinder until the bottom of the organ container contacts a pressurizing component. The pressurizing component, under pressure, introduces internal gas into four airways. As the internal gas pressure increases in the airways, it compresses the sliding plates, causing the four sliding plates to move synchronously towards each other via multiple sliding pillars until the four clamps are completely in contact with the outer wall of the organ container. At this point, rotating the organ container causes the four inserts to rotate until the inserts are inserted into one of the slots at the top of the storage cylinder. After releasing the organ container, the gas is released... The spring force supports the piston plate and pressure seat, causing the organ container to move multiple inserts tightly against the top of the slot's inner wall. The anti-slip texture on the inserts prevents them from rotating. At this time, the four clamps are locked and clamp the organ container, improving the stability of the culture device. Since the range of motion of the clamps is controlled by the downward pressure of the organ container, and each slot has a different height, inserting the inserts into slots of different heights can lock the current position of the clamps, thus limiting the movement of organ containers of different sizes and making the device highly compatible.

[0014] 2. In this in vitro storage and culture device for live organs, when the organ container is clamped by four clamps, the pressure applied by the clamps to the organ container is supported by the air pressure in the airway, which provides a certain buffering effect and prevents the clamps from rigidly squeezing the organ container, causing deformation or even damage to the thin-walled container, thus improving the protective effect. When removing the organ container, the top of the organ container is held and rotated, causing the organ container to rotate the four inserts inside the slots until the inserts disengage from the slots. At the same time, the elastic force of the spring inside the fixed cylinder supports the piston plate, causing the piston plate to push the organ container upward through the pressure seat. As the piston plate moves upward, the volume between the bottom of the fixed cylinder and the piston plate increases, thereby drawing gas from the airway through multiple air guide tubes. This causes the slide plate to move the clamps away from the organ container through the sliding column, thus releasing the clamps from the organ container and facilitating the removal of the organ container, improving the ease of operation. Attached Figure Description

[0015] Figure 1 This is a three-dimensional structural diagram of the present invention;

[0016] Figure 2 This is a schematic diagram of the connection between the organ container and the perfusion culture monitoring system of this utility model;

[0017] Figure 3 This is a schematic diagram of a partial cross-section of the support frame of this utility model;

[0018] Figure 4 This utility model Figure 3 Enlarged structural diagram at point A;

[0019] Figure 5 This is a schematic diagram of the supercharging component of this utility model;

[0020] Figure 6 This is a schematic diagram of a partial cross-section of the storage cylinder of this utility model;

[0021] In the diagram: 1. Refrigerated storage box; 2. Box lid; 3. Control panel; 4. Support frame; 5. Storage cylinder; 6. Organ container; 7. Perfusion tubing; 8. Perfusion culture monitoring system; 9. Limiting component; 901. Clamping plate; 902. Sliding column; 903. Slide plate; 10. Airway; 11. Pressurization component; 111. Pressure seat; 112. Piston plate; 113. Fixing cylinder; 114. Spring; 115. Air duct; 12. Insert; 13. Slot; 14. Handle. Detailed Implementation

[0022] The technical solution of this patent will be further described in detail below with reference to specific embodiments.

[0023] like Figure 1-6As shown, this utility model provides a technical solution: an in vitro storage and culture device for living organs, including a refrigerated storage box 1, a box cover 2 hinged to one side of the refrigerated storage box 1, a control panel 3 installed on the front of the refrigerated storage box 1, and handles 14 respectively provided on both sides of the refrigerated storage box 1. The box cover 2 can cover the opening at the top of the refrigerated storage box 1, improving the protection effect of the internal structure. The handles 14 facilitate the carrying of the refrigerated storage box 1, improving portability.

[0024] A support frame 4 is fixedly connected to the middle of the cold storage box 1. A storage cylinder 5 is fixedly connected through the through holes opened at the four corners of the top of the support frame 4. An organ container 6 is installed inside the storage cylinder 5. The top of the organ container 6 is connected to an infusion pipeline 7. The other ends of the two infusion pipelines 7 on the same side are connected to an infusion culture monitoring system 8. The two infusion culture monitoring systems 8 are respectively installed on both sides inside the cold storage box 1. Four limiting components 9 are attached to the outside of the organ container 6. The limiting components 9 include clamps 901. The clamps 901 are arc-shaped and have anti-slip texture on the inner wall. The bottom ends of the four clamps 901 are located above the pressurizing component 11. The arc-shaped clamps 901 can fully fit the organ container 6 and improve the stability of the clamps 901 in positioning the organ container 6.

[0025] Several sliding pillars 902 are fixed to the outer side of the clamp 901. An air passage 10 is opened in the protruding part inside the storage cylinder 5. The other ends of the sliding pillars 902 pass through the air passage 10 and are fixed with a sliding plate 903. The sliding plate 903 is slidably connected in the air passage 10. A pressurizing component 11 is attached to the bottom of the organ container 6. The four ends of the pressurizing component 11 pass through the storage cylinder 5 and are connected to the four air passages 10. The pressurizing component 11 includes a pressure seat 111. The top of the pressure seat 111 is designed with an arc surface and is flush with the bottom of the organ container 6. The bottom of the pressure seat 111 is fixedly connected to a piston plate 112. A fixed cylinder 113 slides on the outside of the piston plate 112 and is fixed to the bottom of the support frame 4. A spring 114 is fixedly connected to the bottom of the inner wall of the fixed cylinder 113. The top of the spring 114 is fixed to the bottom of the piston plate 112. The elastic force of the spring 114 supports the piston plate 112, so that the piston plate 112 pushes the organ container 6 upward through the pressure seat 111, so as to facilitate the removal of the organ container 6 from the inside of the storage cylinder 5.

[0026] Four air guide pipes 115 are connected to the outside of the fixed cylinder 113. The top end of the air guide pipe 115 passes through the storage cylinder 5 and is connected to the air passage 10. When the pressure seat 111 is pressed, it drives the piston plate 112 at the bottom to move down inside the fixed cylinder 113, thereby allowing the gas inside the fixed cylinder 113 to be introduced into the air passage 10 through the four air guide pipes 115. When the air pressure inside the air passage 10 increases, it squeezes the sliding plate 903, causing the four sliding plates 903 to drive the four clamping plates 901 to move closer to each other synchronously through multiple sliding columns 902, so as to quickly clamp the organ container 6.

[0027] Secondly, the pressure exerted by the clamp 901 on the organ container 6 is supported by the air pressure in the airway 10, which plays a certain buffering role on the organ container 6 and prevents the clamp 901 from rigidly squeezing the organ container 6, causing the thin-walled container to deform or even be damaged.

[0028] Four inserts 12 are fixed on the upper part of the outer wall of the organ container 6. The inserts 12 are provided with anti-slip texture. Several slots 13 are opened in the four protruding parts at the top of the storage cylinder 5. The inserts 12 are inserted into one of the slots 13. The organ container 6 drives the multiple inserts 12 to stick tightly to the top of the inner wall of the slot 13. With the anti-slip texture on the inserts 12, the inserts 12 are prevented from rotating. At this time, the four clamps 901 are locked and clamp the organ container 6 to improve positioning stability.

[0029] The working principle of this utility model is as follows:

[0030] In use, the organ container 6 is connected to the perfusion culture monitoring system 8 via the perfusion tubing 7. The perfusion culture monitoring system 8 simulates in vivo hemodynamics, providing continuous oxygenated nutrient solution perfusion to the organ inside the organ container 6. When removing the organ container 6 from the refrigerated storage box 1, the perfusion tubing 7 is disconnected from the perfusion culture monitoring system 8. Then, the top of the organ container 6 is held and rotated, causing the organ container 6 to rotate the four inserts 12 inside the slots 13 until the inserts 12 disengage from the slots 13. At the same time, the internal spring of the fixing cylinder 113... The elastic force of the spring 114 supports the piston plate 112, causing the piston plate 112 to push the organ container 6 upward through the pressure seat 111. As the piston plate 112 moves upward, the volume between the bottom of the fixed cylinder 113 and the piston plate 112 increases, thereby drawing gas from the airway 10 through multiple air guide tubes 115. This causes the slide plate 903 to move the clamping plate 901 away from the organ container 6 through the sliding column 902, thereby releasing the locking state of the clamping plate 901 on the organ container 6. The organ container 6 can then be removed from the refrigerated storage box 1 by pulling it upward.

[0031] When clamping the organ container 6, insert the bottom of the organ container 6 into the storage cylinder 5 until the bottom of the organ container 6 contacts the pressure seat 111 in the pressurization assembly 11. After the pressure seat 111 is pressed, it drives the piston plate 112 at the bottom to move downward inside the fixed cylinder 113, thereby allowing the gas inside the fixed cylinder 113 to be introduced into the airway 10 through the four air guide pipes 115. When the air pressure inside the airway 10 increases, it squeezes the sliding plate 903, causing the four sliding plates 903 to drive the four clamping plates 901 to move closer to each other synchronously through multiple sliding pillars 902, until the four clamping plates 901 are completely in contact with the outer wall of the organ container 6. At this time, rotate the organ container 6. The organ container 6 rotates along with the four inserts 12 until the inserts 12 are inserted into one of the slots 13 at the top of the storage cylinder 5. After the organ container 6 is released, the spring force of the spring 114 supports the piston plate 112 and the pressure seat 111, causing the organ container 6 to move the multiple inserts 12 to press tightly against the top of the inner wall of the slot 13. The anti-slip texture on the inserts 12 prevents them from rotating on their own. At this time, the four clamps 901 are locked and clamp the organ container 6. The pressure applied by the clamps 901 to the organ container 6 is supported by the air pressure in the airway 10, which provides a certain buffering effect for the organ container 6.

[0032] In the description of this utility model, it should be noted that, unless otherwise explicitly specified and limited, the terms "installation," "connection," and "joining" should be interpreted broadly. For example, they can refer to a fixed connection, a detachable connection, or an integral connection; they can refer to a mechanical connection or an electrical connection; they can refer to a direct connection or an indirect connection through an intermediate medium; and they can refer to the internal connection of two components. Those skilled in the art can understand the specific meaning of the above terms in this utility model based on the specific circumstances.

[0033] The preferred embodiments of this patent have been described in detail above. However, this patent is not limited to the above embodiments. Within the scope of knowledge possessed by those skilled in the art, various changes can be made without departing from the spirit of this patent.

Claims

1. An in vitro storage and culture device for living organs, comprising a refrigerated storage box (1), characterized in that: A support frame (4) is fixedly connected to the middle of the refrigerated storage box (1). A storage cylinder (5) is fixedly connected through the through holes at the four corners of the top of the support frame (4). An organ container (6) is installed inside the storage cylinder (5). An infusion pipeline (7) is connected to the top of the organ container (6). The other ends of the two infusion pipelines (7) on the same side are connected to an infusion culture monitoring system (8). The two infusion culture monitoring systems (8) are installed on both sides of the refrigerated storage box (1). Four limiting components (9) are attached to the outside of the organ container (6). The limiting component (9) includes a clamping plate (901), and several sliding pillars (902) are fixed on the outside of the clamping plate (901). An air passage (10) is opened in the protruding part inside the storage cylinder (5). The other end of the several sliding pillars (902) passes through the air passage (10) and is fixed with a sliding plate (903). The sliding plate (903) is slidably connected in the air passage (10). A pressurizing component (11) is attached to the bottom of the organ container (6). The four ends of the pressurizing component (11) pass through the storage cylinder (5) and are connected to the four air passages (10).

2. The in vitro storage and culture device for living organs according to claim 1, characterized in that: A lid (2) is hinged to one side of the refrigerated storage box (1), a control panel (3) is installed on the front of the refrigerated storage box (1), and handles (14) are provided on both sides of the refrigerated storage box (1).

3. The in vitro storage and culture device for living organs according to claim 1, characterized in that: Four inserts (12) are fixed on the upper part of the outer wall of the organ container (6). The inserts (12) are provided with anti-slip texture. Several slots (13) are opened in the four protruding parts at the top of the storage cylinder (5). The inserts (12) are inserted into one of the slots (13).

4. The in vitro storage and culture device for living organs according to claim 1, characterized in that: The clamps (901) are arc-shaped and have anti-slip texture on the inner wall. The bottom ends of the four clamps (901) are located above the booster assembly (11).

5. The in vitro storage and culture device for living organs according to claim 1, characterized in that: The pressurization assembly (11) includes a pressure seat (111), the top of which is arc-shaped and overlaps with the bottom of the organ container (6). A piston plate (112) is fixedly connected to the bottom of the pressure seat (111), and a fixed cylinder (113) slides outside the piston plate (112). The fixed cylinder (113) is fixed to the bottom of the support frame (4).

6. The in vitro storage and culture device for living organs according to claim 5, characterized in that: A spring (114) is fixedly connected to the bottom of the inner wall of the fixed cylinder (113). The top of the spring (114) is fixed to the bottom of the piston plate (112). Four air guide pipes (115) are connected to the outside of the fixed cylinder (113). The top of the air guide pipes (115) penetrates the storage cylinder (5) and is connected to the air passage (10).

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