A neural stem cell isolation device

Abstract

The utility model discloses a kind of neural stem cell separation devices, including separation device main body, the separation device main body includes host computer, the top of the host computer is equipped with cover plate by hinge, the inside intermediate position of the host computer is formed with inner separation groove, the inside of the inner separation groove is provided with separation cylinder;By design support stability control mechanism, when separation cylinder is separated in rotation, the top of separation cylinder is supported in support control lever, so that rotating block is rotated in the inside of rotating groove control, and when rotating, guiding roller is contacted with the inner surface of rotating groove and rolls smoothly, and is not easily hindered, simultaneously, flexible sealing ring is closed in the surface of rotating groove and is sealed in support rotation, not easily hindered by dirt entering inside, improve the stability of separation cylinder rotation separation in use of separation device main body.

Description

Technical Field

[0001] This utility model belongs to the field of separation device technology, specifically relating to a neural stem cell separation device. Background Technology

[0002] Neural stem cells are a special type of cell in the nervous system that has the ability to self-renew and the potential for multi-lineage differentiation. Neural stem cells need to be isolated and processed in medical research. They are usually isolated and processed using cell separation devices, and the existing separation devices are the equipment used for the isolation of neural stem cells in research.

[0003] Existing separation devices directly introduce neural stem cells into the separation cylinder during neural stem cell separation. A motor drives a reducer to rotate the cylinder for separation. However, during this process, the neural stem cells injected into the cylinder are susceptible to gravity, which drives the bottom of the cylinder, leaving the top hollow and unsupported. This makes it difficult to maintain stability. Furthermore, prolonged separation under significant centrifugal force can cause instability in the center of gravity, resulting in the top of the cylinder swaying and affecting the stability of the separation device during neural stem cell separation. Therefore, this invention proposes a neural stem cell separation device. Utility Model Content

[0004] The purpose of this invention is to provide a neural stem cell separation device to solve the problems mentioned in the background art, where the neural stem cells injected into the separation tube are easily subjected to gravity during the separation process, and the force drives the bottom of the separation tube, making the top of the separation tube hollow and difficult to support stably. Furthermore, during long-term separation, the center of gravity is easily unstable when subjected to large centrifugal forces, resulting in the top of the separation tube swaying and becoming unstable.

[0005] To achieve the above objectives, this utility model provides the following technical solution: a neural stem cell separation device, comprising a separation device body, the separation device body including a main unit, a cover plate mounted on the top of the main unit via a hinge, an inner separation groove formed in the middle of the interior of the main unit, a separation cylinder disposed inside the inner separation groove, a reducer fixed to the bottom of the inner separation groove inside the main unit by bolts, the top of the reducer being fixed to the bottom of the separation cylinder by bolts, a motor disposed on the lower surface of the reducer, and the separation device body further comprising:

[0006] A support and stabilization mechanism is provided, and the support and stabilization mechanism includes a rotating component disposed at the top edge of the separation cylinder. A support connection component is provided at the connection between the end of the rotating component and the inner top of the inner separation groove. Rolling components are provided on both the upper and lower surfaces of the rotating component.

[0007] The material discharge protection mechanism includes a material feeding component located at the bottom of the inner separation tank, a material feeding component located inside the main unit on one side of the material feeding component, and a protection component located at the end of the material feeding component.

[0008] Preferably, the rotating assembly includes a rotating groove formed at the top edge of the separating cylinder, and rotating blocks are provided at both ends of the rotating groove.

[0009] Preferably, the support connection assembly includes a support control rod integrally disposed at the middle position of the outer surface of the rotating block. The end of the support control rod is fixed to the top of the inner surface of the inner separation groove by bolts. Flexible sealing rings are installed on both sides of the outer surface of the rotating groove by screws, and the flexible sealing rings press against the surface of the support control rod.

[0010] Preferably, the rolling assembly includes rolling grooves formed on the upper and lower surfaces of the rotating block, and a guide roller rolls inside the rolling groove, the surface of the guide roller contacting the inner surface of the rotating groove.

[0011] Preferably, the feeding assembly includes an integrally formed feeding arc-shaped surface at the middle position of the bottom end inside the inner separation tank, and an annular inclined groove is formed at the edge of the feeding arc-shaped surface at the inner bottom edge of the inner separation tank.

[0012] Preferably, the feeding assembly includes a discharge pipe located inside the main unit at the bottom end of one side of the annular inclined groove, and the end of the discharge pipe extends to the outside of the main unit.

[0013] Preferably, the protection component includes a threaded structure integrally formed on the end of the discharge pipe, the end of the discharge pipe being threadedly connected to an internal threaded cap through the threaded structure, the inner surface of the internal threaded cap being integrally provided with a plunger, and the outer surface of the plunger being in close contact with the inner surface of the discharge pipe.

[0014] Compared with the prior art, the beneficial effects of this utility model are:

[0015] By designing a support and stabilization mechanism, when the separating cylinder rotates for separation, the top of the separating cylinder, supported by the support control rod, allows the rotating block to rotate and be controlled inside the rotating groove. During rotation, the guide rollers contact the inner surface of the rotating groove and roll smoothly for support, without being easily obstructed. At the same time, the flexible sealing ring is closed on the surface of the rotating groove to provide sealing protection during rotation, preventing dirt from entering and obstructing the interior, thus improving the stability of the main body of the separation device during the rotation and separation of the separating cylinder. Attached Figure Description

[0016] Figure 1 This is a schematic diagram of the structure of this utility model;

[0017] Figure 2 This is a partial cross-sectional view of the inner separation tank, separation cylinder, and main unit of this utility model.

[0018] Figure 3 This utility model Figure 2 Enlarged structural diagram of section A;

[0019] Figure 4 This is a partial cross-sectional view of the rotating block, support control rod and separation cylinder of this utility model;

[0020] Figure 5 This is a partial structural diagram of the rotating block, guide roller, and support control rod of this utility model;

[0021] Figure 6 This utility model Figure 2 Enlarged structural diagram of section B;

[0022] In the diagram: 100, main body of the separation device; 101, main unit; 102, cover plate; 103, inner separation groove; 1031, discharge pipe; 1032, plunger; 1033, internal threaded cover; 1034, annular inclined groove; 1035, feeding arc surface; 1036, threaded structure; 104, separation cylinder; 1041, support control rod; 1042, rotating groove; 1043, flexible sealing ring; 1044, rotating block; 1045, guide roller; 1046, rolling groove; 105, reducer; 106, motor. Detailed Implementation

[0023] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the protection scope of the present utility model.

[0024] Please see Figures 1 to 6This utility model provides a technical solution: a neural stem cell separation device, including a separation device body 100, the separation device body 100 including a main unit 101, a cover plate 102 mounted on the top of the main unit 101 via a hinge, an inner separation groove 103 formed in the middle of the interior of the main unit 101, a separation cylinder 104 disposed inside the inner separation groove 103, a reducer 105 fixed to the bottom of the inner separation groove 103 inside the main unit 101 by bolts, the top of the reducer 105 being fixed to the bottom of the separation cylinder 104 by bolts, a motor 106 disposed on the lower surface of the reducer 105, the motor 106 driving the reducer 105 and the separation cylinder 104 to rotate, the neural stem cells inside the separation cylinder 104 being separated by centrifugal force when the separation cylinder 104 rotates, and the separated stem cells enter the bottom of the inner separation groove 103, while the unseparated stem cells remain inside the separation cylinder 104. The separation device body 100 is also provided with:

[0025] The support and stabilization mechanism includes a rotating component located at the top edge of the separation cylinder 104. A support connection component is provided at the connection between the end of the rotating component and the inner top of the inner separation groove 103. Rolling components are provided on both the upper and lower surfaces of the rotating component. When the separation device body 100 rotates the separation cylinder 104 during the separation process, the support and stabilization mechanism provides stable support for the rotation of the separation cylinder 104, resulting in more stable separation.

[0026] In order to facilitate the stable rotation and separation of the separation cylinder 104 by means of the rotating assembly, in this embodiment, preferably, the rotating assembly includes a rotating groove 1042 formed at the top edge of the separation cylinder 104, and rotating blocks 1044 are provided at both ends of the rotating groove 1042. When the separation cylinder 104 rotates, the rotating blocks 1044 rotate inside the rotating groove 1042 to stably support the rotation of the top of the separation cylinder 104 for separation.

[0027] To facilitate the support and stable rotation of the rotating block 1044 via the support connection assembly, in this embodiment, preferably, the support connection assembly includes a support control rod 1041 integrally disposed at the middle position of the outer surface of the rotating block 1044. The end of the support control rod 1041 is fixed to the top of the inner surface of the inner separation groove 103 by bolts. When the separation cylinder 104 rotates, the rotating block 1044 can be installed and supported for rotation by the support control rod 1041. Flexible sealing rings 1043 are installed on both sides of the outer surface of the rotating groove 1042 by screws. The flexible sealing rings 1043 press against the surface of the support control rod 1041, and the surface of the rotating groove 1042 is protected by the flexible sealing rings 1043 during rotation, making it difficult for dirt to enter the interior.

[0028] In order to facilitate the smooth rotation of the rotating block 1044 by means of the rolling assembly, in this embodiment, preferably, the rolling assembly includes a rolling groove 1046 formed on the upper and lower surfaces of the rotating block 1044, and a guide roller 1045 rolls inside the rolling groove 1046. The surface of the guide roller 1045 contacts the inner surface of the rotating groove 1042, so that the rotating block 1044 can be smoothly rotated and adjusted by the guide roller 1045 when rotating inside the rotating groove 1042, so that the rotation is not easily obstructed.

[0029] The material discharge protection mechanism includes a material feeding component located at the bottom of the inner separation tank 103. A feeding component is located on one side of the material feeding component inside the main unit 101, and a protection component is located at the end of the feeding component. This mechanism facilitates the discharge of material from the separation cylinder 104 after separating neural stem cells, which is located at the bottom of the inner separation tank 103. It also protects the cylinder from the ingress of dirt after use.

[0030] To facilitate the discharge of the separated neural stem cells from the bottom of the inner separation tank 103 via the feeding assembly, in this embodiment, preferably, the feeding assembly includes a feeding arc-shaped surface 1035 integrally formed at the middle position of the bottom of the inner separation tank 103. An annular inclined groove 1034 is formed at the edge of the inner bottom edge of the inner separation tank 103 at the edge of the feeding arc-shaped surface 1035. After separation by the separation cylinder 104, the separated neural stem cells are introduced into the inner bottom of the inner separation tank 103 through the feeding arc-shaped surface 1035 into the interior of the annular inclined groove 1034, and then collected and discharged through the discharge pipe 1031 via the annular inclined groove 1034.

[0031] In order to facilitate the discharge of separated neural stem cells through the feeding assembly, in this embodiment, preferably, the feeding assembly includes a discharge pipe 1031 located inside the host 101 at the bottom end of one side of the annular inclined groove 1034. The end of the discharge pipe 1031 extends to the outside of the host 101, so that the stem cells can be drawn out and collected inside the annular inclined groove 1034 and discharged to the outside through the discharge pipe 1031, which facilitates a cleaner discharge.

[0032] To facilitate the protection of the main body 100 of the separation device when it is not in use after material is discharged from the discharge pipe 1031, in this embodiment, preferably, the protection component includes a threaded structure 1036 integrally formed on the end of the discharge pipe 1031. The end of the discharge pipe 1031 is threadedly connected to an inner threaded cover 1033 through the threaded structure 1036. A plunger 1032 is integrally provided on the inner surface of the inner threaded cover 1033, and the outer surface of the plunger 1032 is in close contact with the inner surface of the discharge pipe 1031. When the main body 100 of the separation device is not in use, the inner threaded cover 1033 can be rotated by the threaded structure 1036 at the end of the discharge pipe 1031, and the rotation of the inner threaded cover 1033 will drive the plunger 1032 to be embedded in the interior of the discharge pipe 1031 for sealing and protection.

[0033] The working principle and usage process of this utility model are as follows: When using this neural stem cell separation device, the main body 100 of the separation device is first placed stably at the usage position through the bottom end of the host 101. When using the main body 100 of the separation device, neural stem cells are injected into the separation cylinder 104. The cover plate 102 is closed for protection. The motor 106 drives the reducer 105 to rotate with the separation cylinder 104. When the separation cylinder 104 rotates, the neural stem cells inside are separated according to the centrifugal force. The separated ones enter the bottom of the inner separation tank 103, while the unseparated ones remain inside the separation cylinder 104.

[0034] Then, the separated neural stem cells are introduced into the bottom of the inner separation tank 103, and then introduced into the inner edge of the annular inclined groove 1034 through the feeding arc surface 1035. The annular inclined groove 1034 is inclined at an angle to guide the material to the bottom and into the discharge pipe 1031 for discharge. The separated neural stem cells are discharged more conveniently and more cleanly. When not in use, after cleaning the inside, the inner threaded cover 1033 is rotated to the end of the discharge pipe 1031 through the threaded structure 1036. When the inner threaded cover 1033 is rotated, the plunger 1032 is embedded into the inside of the discharge pipe 1031 for sealing installation, which facilitates sealing protection and improves the convenience of feeding and discharging the main body 100 of the separation device during use and the protection effect on the discharge pipe 1031 after use.

[0035] Finally, when the separating cylinder 104 rotates to separate, the top of the separating cylinder 104 is supported by the support control rod 1041, so that the rotating block 1044 rotates inside the rotating groove 1042. When rotating, it drives the guide roller 1045 to contact the inner surface of the rotating groove 1042 and roll smoothly to support the rotation. It is not easily obstructed. At the same time, the flexible sealing ring 1043 is closed on the surface of the rotating groove 1042 to facilitate sealing and protection when supporting the rotation. It is not easy for dirt to enter and be obstructed, thus improving the stability of the separation device body 100 in supporting the rotating separation of the separating cylinder 104 during use.

[0036] Although embodiments of the present invention have been shown and described (see the detailed description above), it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made to these embodiments without departing from the principles and spirit of the present invention, the scope of which is defined by the appended claims and their equivalents.

Claims

1. A neural stem cell separation device, comprising a separation device body (100), the separation device body (100) including a main unit (101), a cover plate (102) hinged to the top of the main unit (101), an inner separation groove (103) formed in the middle of the interior of the main unit (101), a separation cylinder (104) disposed inside the inner separation groove (103), a reducer (105) fixed to the bottom of the inner separation groove (103) inside the main unit (101) by bolts, the top of the reducer (105) being fixed to the bottom of the separation cylinder (104) by bolts, and a motor (106) disposed on the lower surface of the reducer (105), characterized in that: The main body (100) of the separation device is also provided with: The support and stabilization mechanism includes a rotating component disposed at the top edge of the separation cylinder (104), and a support connection component is provided at the connection between the end of the rotating component and the inner top of the inner separation groove (103). Rolling components are provided on both the upper and lower surfaces of the rotating component. The material discharge protection mechanism includes a material feeding component located at the bottom of the inner separation tank (103), a material feeding component located on one side of the material feeding component inside the host (101), and a protection component located at the end of the material feeding component.

2. The neural stem cell separation device according to claim 1, characterized in that: The rotating assembly includes a rotating groove (1042) opened at the top edge of the separating cylinder (104), and rotating blocks (1044) are provided at both ends of the interior of the rotating groove (1042).

3. The neural stem cell separation device according to claim 2, characterized in that: The support connection assembly includes a support control rod (1041) integrally disposed at the middle position of the outer surface of the rotating block (1044). The end of the support control rod (1041) is fixed to the top of the inner surface of the inner separation groove (103) by bolts. Flexible sealing rings (1043) are installed on both sides of the outer surface of the rotating groove (1042) by screws, and the flexible sealing rings (1043) press against the surface of the support control rod (1041).

4. The neural stem cell separation device according to claim 2, characterized in that: The rolling assembly includes rolling grooves (1046) formed on the upper and lower surfaces of the rotating block (1044), and a guide roller (1045) rolls inside the rolling groove (1046), the surface of the guide roller (1045) being in contact with the inner surface of the rotating groove (1042).

5. The neural stem cell separation device according to claim 1, characterized in that: The feeding assembly includes a feeding arc-shaped surface (1035) integrally formed at the middle position of the bottom end inside the inner separation groove (103), and an annular inclined groove (1034) is opened at the edge of the feeding arc-shaped surface (1035) at the bottom edge inside the inner separation groove (103).

6. The neural stem cell separation device according to claim 5, characterized in that: The feeding assembly includes a discharge pipe (1031) with its bottom end located inside the main unit (101) on one side of the annular inclined groove (1034), and the end of the discharge pipe (1031) extends to the outside of the main unit (101).

7. The neural stem cell separation device according to claim 6, characterized in that: The protective component includes a threaded structure (1036) integrally formed on the end of the discharge pipe (1031). The end of the discharge pipe (1031) is threadedly connected to an internal threaded cap (1033) through the threaded structure (1036). A plunger (1032) is integrally provided on the inner surface of the internal threaded cap (1033), and the outer surface of the plunger (1032) is in close contact with the inner surface of the discharge pipe (1031).

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