A glutamate dehydrogenase assay kit

Abstract

The utility model relates to the technical field of kit discloses a glutamate dehydrogenase determination kit, including the outer box, the inside of outer box is provided with fixed mechanism, the bottom sliding connection of micro -hole board cover has the reaction board, the fixed mechanism includes fixed block, the outside fixed connection of fixed block in the inside of outer box, the inside fixed connection of outer box has the supporting plate, the outside fixed inside of supporting plate is provided with a plurality of limit components, the inside fixed connection of outer box has a plurality of baffle blocks, a plurality of the outside both sides fixed connection of baffle block has two clamps. In the utility model, the reaction board is limited from the outside through fixed block, and the reagent bottle is tightly wrapped by the elastic rubber ring on the supporting plate, and the clamps on both sides of the baffle block are further clamped from the transverse direction, so that the problems of reagent leakage and reaction plate displacement caused by vibration are effectively avoided, and double protection is provided for the stability of the detection process.

Description

Technical Field

[0001] This invention relates to the field of reagent kit technology, and in particular to a glutamate dehydrogenase assay kit. Background Technology

[0002] In the clinical laboratory, medical staff test a batch of physical examination samples for glutamate dehydrogenase levels using a glutamate dehydrogenase assay kit. After the samples are sent to the lab, the technicians quickly complete steps such as sample dilution, reagent addition, and reaction incubation according to standard operating procedures. The data is then read using the testing instrument, providing crucial information for the diagnosis of diseases such as liver dysfunction. Glutamate dehydrogenase assay kits are commercially available tools for the in vitro quantitative detection of glutamate dehydrogenase activity in biological samples. They typically include components such as substrates, coenzymes, buffer solutions, and reaction plates required for the enzyme reaction, providing a detection method for clinical diagnosis and research experiments.

[0003] Existing glutamate dehydrogenase assay kits primarily operate based on the specificity and measurability of enzymatic reactions. The substrate in the kit undergoes a specific chemical reaction under the catalysis of glutamate dehydrogenase, accompanied by a change in the redox state of the coenzyme. This change can be measured by absorbance at a specific wavelength using instruments such as a spectrophotometer. By measuring the rate or magnitude of the absorbance change in the reaction system and combining this with a standard curve, the activity level of glutamate dehydrogenase in the sample can be calculated.

[0004] However, in the existing technology, some glutamate dehydrogenase assay kits often experience reagent leakage and reaction plate displacement due to vibration during transportation. The packaging structure of these kits lacks effective fixation and cushioning. The reagent bottles are usually just loosely placed in the packaging box. When encountering bumps, collisions, or other vibrations during transportation, the reagent bottles are prone to tipping over and colliding with each other, which can lead to bottle breakage or loosening of the cap, causing reagent leakage and rendering the entire kit ineffective. In response to the above problems, a glutamate dehydrogenase assay kit is proposed. Utility Model Content

[0005] To overcome the above deficiencies, this invention provides a glutamate dehydrogenase assay kit, which aims to improve the problems of reagent leakage and reaction plate displacement caused by vibration during transportation of some existing assay kits.

[0006] To achieve the above objectives, the present invention adopts the following technical solution:

[0007] A glutamate dehydrogenase assay kit includes an outer box, a protective mechanism inside the outer box, a fixing mechanism inside the outer box, a cover plate slidably connected to the top inside the outer box, a light-shielding plate fixedly connected to the bottom of the cover plate, a microwell plate cover slidably connected to the bottom of the light-shielding plate, a reaction plate slidably connected to the bottom of the microwell plate cover, the fixing mechanism including a fixing block, the fixing block being fixedly connected to the outside of the outer box, a support plate being fixedly connected to the inside of the outer box, multiple limiting components being fixedly provided on the outside of the support plate, multiple stops being fixedly connected to the bottom inside the outer box, and two clips being fixedly connected to the outer sides of the multiple stops.

[0008] As a further description of the above technical solution:

[0009] The protective mechanism includes a protective layer, the outer side of which is fixedly connected to the inner side of the outer box, and a buffer layer is fixedly connected to the outer side of the protective layer.

[0010] As a further description of the above technical solution:

[0011] The fixing block is slidably connected to the outside of the reaction plate, and a reagent bottle is slidably connected between the two clamps;

[0012] As a further description of the above technical solution:

[0013] The limiting component includes a rubber ring, the outer side of which is opened inside the support plate, and the limiting component has multiple fixing holes fixedly connected inside;

[0014] As a further description of the above technical solution:

[0015] An insulation layer is fixedly connected to the outside of the buffer layer, and the inner side of the insulation layer is fixedly connected to the outside of the support plate.

[0016] As a further description of the above technical solution:

[0017] A pad is provided at the bottom of the reaction plate, and the bottom of the pad is slidably connected to the top of the support plate.

[0018] This utility model has the following beneficial effects:

[0019] 1. In this utility model, the fixing block limits the reaction plate from the outside, and the rubber ring on the support plate tightly wraps the reagent bottle with elasticity. The clamps on both sides of the block further clamp it from the side. The three work together to improve the anti-shaking ability of the reagent and reaction plate during transportation and storage, effectively avoiding the problems of reagent leakage and reaction plate displacement caused by vibration. At the same time, the elastic design of the rubber ring and clamps makes it easy to take out the reagent without complicated operation, simply by inserting and removing it, ensuring the reliability of the fixation and providing double protection for the stability and convenience of the detection process.

[0020] 2. In this utility model, the protective layer is directly attached to the inner side of the outer box, which can effectively isolate the intrusion of external dust, moisture and other pollutants. The buffer layer absorbs external vibrations during transportation or operation through its own elastic structure. In addition, it works with the fixing mechanism to reduce the impact on reagent bottles and reaction plates, reducing the risk of reagent leakage or interference with the reaction system. The heat insulation layer tightly wraps the support plate, which can maintain a relatively stable temperature inside the box, reduce the impact of external temperature fluctuations on reagent activity, extend the shelf life of reagents, and ensure the reliability of test results from the perspective of the external environment. Attached Figure Description

[0021] Figure 1 This is a three-dimensional schematic diagram of a glutamate dehydrogenase assay kit proposed in this utility model;

[0022] Figure 2 This is a schematic diagram of the reaction plate structure of a glutamate dehydrogenase assay kit proposed in this utility model;

[0023] Figure 3 This is a schematic diagram of the buffer layer structure of a glutamate dehydrogenase assay kit proposed in this utility model;

[0024] Figure 4 for Figure 1 Enlarged view of point A in the middle.

[0025] Legend:

[0026] 1. Outer box; 2. Cover plate; 3. Light shield; 4. Microporous plate cover; 5. Reaction plate; 6. Pad plate; 7. Fixing mechanism; 71. Fixing block; 72. Limiting component; 721. Fixing hole; 722. Rubber ring; 73. Clip; 74. Support plate; 75. Stop block; 8. Protective mechanism; 81. Insulation layer; 82. Buffer layer; 83. Protective layer. Detailed Implementation

[0027] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the protection scope of the present utility model.

[0028] Reference Figures 1 to 3 This utility model provides an embodiment of a glutamate dehydrogenase assay kit, comprising an outer box 1, with a protective mechanism 8 inside the outer box 1. The protective mechanism 8 provides environmental protection for the internal reagents through the synergistic effect of a multi-layer structure. A fixing mechanism 7 is provided inside the outer box 1 to stabilize and fix the reagents and reaction plate 5 to prevent displacement or shaking during transportation and use. The outer box 1 provides overall support and a closed space to protect the internal components from external physical damage and environmental interference. A cover plate 2 is slidably connected to the top inside the outer box 1. The cover plate 2 can slide along the outer box 1 to open and close, providing both protection and convenient operation. A light-shielding plate 3 is fixedly connected to the bottom of the cover plate 2. The light-shielding plate 3 is made of opaque material. A microwell plate cover 4 is slidably connected to the bottom of the light-shielding plate 3. A reaction plate 5 is slidably connected to the bottom of the microwell plate cover 4. The microwell plate cover 4 fits into the reaction plate 5. The reaction plate 5 is provided with reaction holes, providing a standardized container for reagent mixing and enzymatic reaction, facilitating batch detection operations.

[0029] The fixing mechanism 7 includes fixing blocks 71, which are symmetrically distributed on the side of the reaction plate 5. They rigidly limit the reaction plate 5 from the outside to prevent the reaction plate 5 from shifting laterally during operation or movement. The fixing blocks 71 are externally fixedly connected to the inside of the outer box 1. The support plate 74 is fixedly connected to the inside of the outer box 1. The support plate 74 maintains the stability and layering of the internal structure. Multiple limiting components 72 are set inside the support plate 74. The limiting components 72 achieve tight clamping through an elastic structure. Multiple stops 75 are fixedly connected to the bottom inside the outer box 1. Two clips 73 are fixedly connected to the outer sides of the multiple stops 75. The clips 73 are made of elastic material and apply clamping force from both sides of the reagent bottle. They are fixed in the direction of the stops 75 and rubber rings 722. The stops 75 support the reagent bottle from the bottom and limit its longitudinal displacement. They are fixed in three dimensions with the clips 73. The fixing blocks 71 are externally slidably connected to the outside of the reaction plate 5. The reagent bottle is slidably connected between the two clips 73.

[0030] The limiting component 72 includes a rubber ring 722, the outer side of which is opened inside the support plate 74. The rubber ring 722 has elastic deformation capability and is fitted on the outside of the reagent bottle. It fits tightly against the bottle body through friction, buffering vibration and enhancing the fixing effect. The limiting component 72 has multiple fixing holes 721 fixedly connected inside. The fixing holes 721 match the size of the reagent bottle, providing a precise placement position for the reagent bottle and avoiding confusion between different reagents.

[0031] Reference Figures 1 to 3 The protective mechanism 8 includes a protective layer 83, which is externally fixedly connected to the inside of the outer box 1. A buffer layer 82 is also externally fixedly connected to the protective layer 83. The protective layer 83 is made of corrosion-resistant and wear-resistant material and directly contacts the wall of the outer box 1, preventing external moisture, dust, and other contaminants from entering the box. An insulation layer 81 is externally fixedly connected to the buffer layer 82. The buffer layer 82 is made of elastic material such as sponge or foam, which absorbs the impact force generated during transportation or collision and reduces the vibration impact on internal components. The inner side of the insulation layer 81 is fixedly connected to the outer side of the support plate 74. The insulation layer 81 uses heat insulation material to reduce the temperature exchange between the inside and outside of the box, maintain the internal temperature stability, and protect the activity of reagents. A pad 6 is provided at the bottom of the reaction plate 5. The bottom of the pad 6 is slidably connected to the top of the support plate 74. The pad 6 is made of soft elastic material and is placed on the support plate 74 of the reaction plate 5 to buffer the pressure and vibration during sample addition and prevent the reaction plate 5 from being damaged by hard contact.

[0032] Working principle: The operator slides the cover plate 2 out from the top inside of the outer box 1, so that the cover plate 2 and the light shield 3 move synchronously, exposing the internal reaction plate 5 and the fixing mechanism 7. First, remove the microporous plate cover 4 and the reaction plate 5 that are blocked by the fixing block 71. Then, remove the pad 6. Then, take out the reagent from the fixing hole 721 on the support plate 74. The rubber ring 722, the clip 73 and the stop block 75 are reinforced with a compound to ensure that the reagent will not shake during transportation and storage.

[0033] When taking reagents, pull out the reagent bottle; the clip 73 and rubber ring 722 will automatically loosen due to elasticity, making it easy to remove. During the detection operation, add the reagents to the reaction plate 5 in proportion. The pad 6 of the reaction plate 5 acts as a buffer to prevent vibration during sample addition from affecting the reaction system. At the same time, the fixing block 71 limits the reaction plate 5 from the outside to prevent it from shifting. If the detection requires light protection, the light shield 3 can be used. After the sample is added, slide the microplate cover 4 to cover the reaction plate 5 to prevent dust contamination or reagent evaporation. The protective layer 83 isolates external contaminants, the buffer layer 82 absorbs external vibrations, and the heat insulation layer 81 maintains a stable internal temperature, providing a suitable environment for the reagent reaction.

[0034] Finally, it should be noted that the above description is only a preferred embodiment of the present utility model and is not intended to limit the present utility model. Although the present utility model has been described in detail with reference to the foregoing embodiments, those skilled in the art can still modify the technical solutions described in the foregoing embodiments or make equivalent substitutions for some of the technical features. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of the present utility model should be included within the protection scope of the present utility model.

Claims

1. A glutamate dehydrogenase assay kit, comprising an outer box (1), characterized in that: The outer box (1) is provided with a protective mechanism (8) inside, and a fixing mechanism (7) is provided inside. The top side of the outer box (1) is slidably connected to a cover plate (2). The bottom of the cover plate (2) is fixedly connected to a light shield plate (3). The bottom of the light shield plate (3) is slidably connected to a microporous plate cover (4). The bottom of the microporous plate cover (4) is slidably connected to a reaction plate (5). The fixing mechanism (7) includes a fixing block (71), which is fixedly connected to the outside of the outer box (1). A support plate (74) is fixedly connected to the inside of the outer box (1). Multiple limiting components (72) are fixedly arranged inside the support plate (74). Multiple stops (75) are fixedly connected to the bottom inside the outer box (1). Two clips (73) are fixedly connected to the outer sides of the multiple stops (75).

2. The glutamate dehydrogenase assay kit according to claim 1, characterized in that: The protective mechanism (8) includes a protective layer (83), the outer side of which is fixedly connected to the inner side of the outer box (1), and a buffer layer (82) is fixedly connected to the outer side of the protective layer (83).

3. The glutamate dehydrogenase assay kit according to claim 2, characterized in that: The fixing block (71) is slidably connected to the outside of the reaction plate (5), and a reagent bottle is slidably connected between the two clips (73).

4. The glutamate dehydrogenase assay kit according to claim 3, characterized in that: The limiting component (72) includes a rubber ring (722), the outside of which is opened inside the support plate (74), and the inside of the limiting component (72) is fixedly connected with a plurality of fixing holes (721).

5. The glutamate dehydrogenase assay kit according to claim 4, characterized in that: The buffer layer (82) is fixedly connected to the outside of the insulation layer (81), and the inside of the insulation layer (81) is fixedly connected to the outside of the support plate (74).

6. The glutamate dehydrogenase assay kit according to claim 5, characterized in that: The bottom of the reaction plate (5) is provided with a pad (6), and the bottom of the pad (6) is slidably connected to the top of the support plate (74).

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