A high-throughput nucleic acid chip purification device

Abstract

The utility model discloses a high -flux nucleic acid chip purification device, including purifying column and waste liquid pipe, be equipped with the limit seat on waste liquid pipe, purifying column upper end is through the limit plate and is connected in the limit groove of limit seat upper part, and the outside of waste liquid pipe is slid and is equipped with the connecting seat, and the outside of connecting seat is rotated and is equipped with the locating support through the connecting shaft, and the inwall of locating support is slid and is equipped with purifying seal compression pad through the limit sliding hole and the limit sliding block, and one end of locating support is still threaded and is equipped with the locking bolt of vertical contact with purifying seal compression pad, the utility model discloses the connecting seat, connecting shaft and locating support of being equipped with outside waste liquid pipe, and the inwall of locating support is slid and is equipped with purifying seal compression pad, so that through the rotation locking bolt pressure and is equipped with purifying seal compression pad outside one end of purifying column, the stability and the sealing of purifying seal compression pad and are connected in one end of purifying column are convenient to improve, effectively prevent the liquid seepage when centrifugal purification of purifying column upper part.

Description

Technical Field

[0001] This invention relates to the field of nucleic acid chip purification technology, specifically a high-throughput nucleic acid chip purification device. Background Technology

[0002] Biochips, also known as nucleic acid chips or gene chips, are a technology that involves immobilizing a large number of probe molecules on a support and then hybridizing them with fluorescently labeled DNA or other sample molecules. The quantity and sequence information of the sample molecules are obtained by detecting the hybridization signal intensity of each probe molecule. In the fields of genetic engineering and protein engineering, nucleic acid molecules are the main research objects, and the isolation and linearization of nucleic acids are fundamental techniques in nucleic acid research. Nucleic acids are the basic units representing the genetic characteristics of living organisms. Nucleic acid detection is an advanced biological detection at the molecular level. In the field of biology, purification columns are frequently used to extract nucleic acids from samples. Nucleic acid isolation refers to separating nucleic acids from biomolecules such as proteins, polysaccharides, and fats. Currently, centrifugation is commonly used to separate nucleic acids of different specific gravities from proteins and other impurities to achieve purification.

[0003] A novel microporous purification column, with application number 202321712505.1, includes a purification column body with a waste liquid tube slidably disposed at the outer end of the purification column body. In this invention, four limiting blocks and four limiting grooves are slidably connected to limit the position of the purification column body and the waste liquid tube, preventing rotation during centrifugation and filtration that could lead to liquid leakage. Furthermore, the use of a sealing ring and sealing groove effectively prevents leakage at the connection between the purification column body and the waste liquid tube. However, the stability and sealing performance of sealing one end of the purification column with a sealing cap are poor, leading to easy liquid seepage from the upper part of the purification column during centrifugation purification. Utility Model Content

[0004] The purpose of this invention is to provide a high-throughput nucleic acid chip purification device to solve the problem that the stability and sealing of the purification column are poor when one end is sealed with a sealing cap, which leads to liquid leakage during centrifugation purification of the upper part of the purification column.

[0005] To achieve the above objectives, this utility model provides the following technical solution: a high-throughput nucleic acid chip purification device, comprising a purification column and a waste liquid tube, wherein a limiting seat is provided on the upper side of the waste liquid tube, and the upper end of the purification column is engaged in the limiting groove on the upper part of the limiting seat by a limiting plate, a connecting seat is slidably provided on the outer side of the waste liquid tube, and a positioning bracket is rotatably provided on the outer side of the connecting seat by a connecting shaft, and a purification sealing pressure plate is slidably provided on the inner wall of the positioning bracket through a limiting sliding hole and a limiting slider, and a locking bolt is threaded on one end of the positioning bracket and perpendicularly contacts the purification sealing pressure plate.

[0006] Furthermore, the purification column is provided with an inlet at the upper end and a filter screen at the bottom.

[0007] Furthermore, a sealing groove is provided at the bottom of the limiting groove, and a sealing rib is provided on the lower side of the limiting plate to engage with the sealing groove.

[0008] Furthermore, a sealing plug is provided on one side of the purification sealing plate, and a sealing ring is fitted on the outside of the sealing plug.

[0009] Furthermore, the limiting slider is a rectangular slider, and the two sides of the limiting slider are slidably arranged with the limiting sliding hole.

[0010] Furthermore, the limiting sliding hole is also provided with a guide rod that slides with the limiting slider, and a spring is sleeved on the outer side of one end of the guide rod.

[0011] Compared with the prior art, the beneficial effects of this utility model are:

[0012] 1. This utility model has a connecting seat, connecting shaft and positioning bracket on the outside of the waste liquid pipe. The inner wall of the positioning bracket is equipped with a purification sealing plate, which is pressed against the outside of the purification sealing plate at one end of the purification column by rotating the locking bolt. This improves the stability and sealing of the purification sealing plate at one end of the purification column and effectively prevents liquid leakage during centrifugation purification at the top of the purification column.

[0013] 2. This utility model has a sealing groove at the bottom of the limiting groove and a sealing rib on the lower side of the limiting plate that engages with the sealing groove, which increases the sealing performance of the purification column when it is connected to the waste liquid pipe. In addition, a sealing plug is provided on one side of the purification sealing pressure plate and a sealing ring is fitted on the outside of the sealing plug, which increases the sealing performance of the connection between the purification column and the purification sealing pressure plate.

[0014] 3. This utility model has a connecting seat that slides on the outside of the waste liquid pipe, and a positioning bracket that rotates on the outside of the connecting seat via a connecting shaft. When not in use, the positioning bracket can be flipped and adjusted to the side of the waste liquid pipe. At the same time, the connecting seat and the positioning bracket can be disassembled when not in use, which improves the flexibility of the purification sealing plate. Attached Figure Description

[0015] The accompanying drawings are provided to further illustrate the present invention and form part of the specification. They are used together with the embodiments of the present invention to explain the present invention, but do not constitute a limitation thereof. In the drawings:

[0016] Figure 1 This is a schematic diagram of the overall structure of this utility model;

[0017] Figure 2 This is a schematic diagram of the disassembly structure of the purification column of this utility model;

[0018] Figure 3 This is a schematic diagram of the purification column structure of this utility model;

[0019] Figure 4 This is a schematic diagram of the positioning bracket structure of this utility model.

[0020] In the diagram: 1. Waste liquid pipe; 2. Limiting seat; 3. Limiting groove; 4. Sealing groove; 5. Purification column; 6. Limiting plate; 7. Sealing rib; 8. Connecting shaft; 9. Positioning bracket; 10. Guide rod; 11. Limiting slider; 12. Spring; 13. Limiting sliding hole; 14. Locking bolt; 15. Filter screen; 16. Connecting seat; 17. Purification sealing pressure plate; 18. Sealing ring. Detailed Implementation

[0021] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the protection scope of the present utility model.

[0022] Please see Figure 1 , Figure 2 , Figure 3 , Figure 4 In this embodiment of the present invention, a high-throughput nucleic acid chip purification device includes a purification column 5, a purification sealing plate 17, and a waste liquid pipe 1. The purification column 5 has an inlet at its upper end and a filter screen 15 at its bottom. The waste liquid pipe 1 has a limiting seat 2 on its upper side. The upper end of the purification column 5 is engaged in the limiting groove 3 on the upper part of the limiting seat 2 by a limiting plate 6. A connecting seat 16 is slidably provided on the outside of the waste liquid pipe 1, and a positioning bracket 9 is rotatably provided on the outside of the connecting seat 16 through a connecting shaft 8. This allows the positioning bracket 9 to be flipped and adjusted to the side of the waste liquid pipe 1 when not in use. At the same time, the connecting seat 16 and the positioning bracket 9 can be disassembled when not in use, improving the flexibility of the purification sealing plate 17.

[0023] like Figure 1 and Figure 2As shown, in order to improve the stability and sealing of the purification sealing plate 17 when it is engaged with one end of the purification column 5, the purification sealing plate 17 is also slidably provided on the inner wall of the positioning bracket 9 through the limiting sliding hole 13 and the limiting slider 11. The limiting slider 11 is a rectangular slider, and the two sides of the limiting slider 11 are slidably arranged with the limiting sliding hole 13. One end of the positioning bracket 9 is also threaded with a locking bolt 14 that is perpendicularly in contact with the purification sealing plate 17. The limiting sliding hole 13 is also provided with a guide rod 10 that is slidably arranged with the limiting slider 11. A spring 12 is sleeved on the outer side of one end of the guide rod 10, so that by rotating the locking bolt 14, the purification sealing plate 17 is pressed on the outer side of one end of the purification column 5, which facilitates the stability and sealing of the purification sealing plate 17 when it is engaged with one end of the purification column 5, effectively preventing liquid leakage during centrifugation purification at the top of the purification column. Moreover, by rotating the locking bolt 14 in the opposite direction, the purification sealing plate 17 is disengaged from one end of the purification column 5 under the action of the spring 12, thus facilitating the disassembly of the purification sealing plate 17.

[0024] like Figure 2 and Figure 3 As shown, a sealing groove 4 is also provided at the bottom of the limiting groove 3, and a sealing rib 7 is provided on the lower side of the limiting plate 6 to engage with the sealing groove 4, which increases the sealing performance of the purification column 5 when it is engaged with the waste liquid pipe 1; a sealing plug is provided on one side of the purification sealing pressure plate 17, and a sealing ring 18 is provided on the outside of the sealing plug, which increases the sealing performance of the purification column 5 when it is inserted into the purification sealing pressure plate 17.

[0025] The working principle and usage process of this utility model are as follows: In use, the connecting seat 16, connecting shaft 8 and positioning bracket 9 are provided on the outside of the waste liquid pipe 1. The inner wall of the positioning bracket 9 is slidably provided with a purification sealing plate 17. One end of the positioning bracket 9 is also threaded with a locking bolt 14 that is in perpendicular contact with the purification sealing plate 17. By rotating the locking bolt 14, the purification sealing plate 17 is pressed against the outside of one end of the purification column 5, which facilitates the stability and sealing of the purification sealing plate 17 in the purification column 5 and effectively prevents liquid leakage during centrifugation purification at the top of the purification column. Moreover, by rotating the locking bolt 14 in the opposite direction, the purification sealing plate 17 is disengaged from one end of the purification column 5 under the action of the spring 12, thereby facilitating the disassembly of the purification sealing plate 17.

[0026] Finally, it should be noted that the above description is merely a preferred embodiment of this utility model and is not intended to limit the utility model. Although the utility model has been described in detail with reference to the foregoing embodiments, those skilled in the art can still modify the technical solutions described in the foregoing embodiments or make equivalent substitutions for some of the technical features. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of this utility model should be included within the protection scope of this utility model.

Claims

1. A high-throughput nucleic acid chip purification device, comprising a purification column (5) and a waste liquid tube (1), characterized in that: The waste liquid pipe (1) is provided with a limiting seat (2) on the upper side. The upper end of the purification column (5) is clamped in the limiting groove (3) on the upper part of the limiting seat (2) by the limiting plate (6). The waste liquid pipe (1) is provided with a connecting seat (16) on the outer side. The connecting seat (16) is provided with a positioning bracket (9) on the outer side by a connecting shaft (8). The inner wall of the positioning bracket (9) is provided with a purification sealing pressure plate (17) through a limiting sliding hole (13) and a limiting slider (11). One end of the positioning bracket (9) is also provided with a locking bolt (14) that is in perpendicular contact with the purification sealing pressure plate (17).

2. The high-throughput nucleic acid chip purification device according to claim 1, characterized in that: The purification column (5) is provided with an inlet at the upper end and a filter screen (15) at the bottom.

3. The high-throughput nucleic acid chip purification device according to claim 1, characterized in that: The bottom of the limiting groove (3) is provided with a sealing groove (4), and the lower side of the limiting plate (6) is provided with a sealing rib (7) that engages with the sealing groove (4).

4. The high-throughput nucleic acid chip purification device according to claim 1, characterized in that: The purification sealing plate (17) has a sealing plug on one side, and a sealing ring (18) is fitted on the outside of the sealing plug.

5. The high-throughput nucleic acid chip purification device according to claim 1, characterized in that: The limiting slider (11) is a rectangular slider, and the two sides of the limiting slider (11) are slidably connected to the limiting sliding hole (13).

6. The high-throughput nucleic acid chip purification device according to claim 5, characterized in that: The limiting sliding hole (13) is also provided with a guide rod (10) that slides with the limiting slider (11), and a spring (12) is sleeved on the outer side of one end of the guide rod (10).

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