A nucleic acid extraction device

Abstract

The utility model discloses a nucleic acid extraction device relates to nucleic acid extraction equipment technical field, including magnetism stick cover, magnetism stick subassembly, magnetism stick support subassembly and drive assembly, magnetism stick subassembly includes magnetism stick cover fixed base and magnetism stick, and magnetism stick cover fixed base swing sleeve joint is in on magnetism stick, and drive assembly drives magnetism stick support subassembly to move up and down, and magnetism stick support subassembly drives magnetism stick to move up and down, and magnetism stick cover is assembled with magnetism stick. The device whole process of the utility model will not appear magnetism stick cover shift or fall off, has improved the assembly stability between each structure and the efficiency of device detection.

Description

Technical Field

[0001] This utility model relates to the field of nucleic acid extraction equipment technology, and in particular to a nucleic acid extraction device. Background Technology

[0002] With the gradual advancement of medical science testing technology, nucleic acid testing has been widely used. Therefore, efficient and large-scale extraction of nucleic acid from biological samples has become a prerequisite for scientific research and medical diagnosis. Currently, nucleic acid extraction technology using magnetic beads as adsorption carriers has been widely applied due to its advantages such as high throughput and high sensitivity. At present, fully automated nucleic acid extractors based on magnetic bead method are mainly divided into two categories: magnetic rod type and pipette type. Among them, magnetic rod type nucleic acid extractors are favored by scientific research and medical users for their advantages such as small size, simple operation and short extraction time.

[0003] The magnetic rod sleeve for nucleic acid extraction is a component used for isolation and mixing in magnetic rod-type nucleic acid extraction. A magnetic rod is inserted into the sleeve to make it magnetic, attracting magnetically attracted particles in the mixture and adsorbing them onto the surface of the sleeve. When the magnetic rod is removed, the sleeve loses its magnetism, and the magnetically attracted particles detach from the sleeve, thus avoiding direct contact between the magnetic rod and the mixture.

[0004] Currently available magnetic rod sleeves may not be securely fastened due to the high-frequency movement of nucleic acid extraction instruments during actual use. This can cause the magnetic rod sleeve to shift or fall off, affecting the instrument's detection progress. Utility Model Content

[0005] To address the existing problems, this invention provides a nucleic acid extraction device.

[0006] To achieve the objectives of this utility model, the technical solution adopted is as follows:

[0007] A nucleic acid extraction device includes a magnetic rod sleeve, a magnetic rod assembly, a magnetic rod support assembly, and a driving assembly; the magnetic rod assembly includes a magnetic rod sleeve fixing seat and a magnetic rod, the magnetic rod sleeve fixing seat being movably sleeved on the magnetic rod; and the driving assembly drives the magnetic rod support assembly to move up and down, the magnetic rod support assembly driving the magnetic rod to move up and down; the magnetic rod sleeve and the magnetic rod are assembled.

[0008] Based on the above technical solution, the magnetic rod support assembly further includes a magnetic rod seat support plate and a magnetic rod fixing plate, with the magnetic rod seat support plate located below the magnetic rod fixing plate.

[0009] Based on the above technical solution, the drive assembly further includes a drive motor, a motor lead screw and a lead screw nut, and the power output shaft of the drive motor is connected to the motor lead screw, the motor lead screw passes through the lead screw nut and extends to connect with the magnetic rod seat support plate.

[0010] Based on the above technical solution, the magnetic rod sleeve is further provided with ribs and notches, with the ribs distributed on the outer surface of the magnetic rod sleeve and the notches opened on the upper edge of the magnetic rod sleeve.

[0011] Based on the above technical solution, the magnetic rod sleeve fixing base is further provided with an inlet angle.

[0012] Based on the above technical solution, further, the upper inner surface of the magnetic rod sleeve is provided with a boss, and the magnetic rod sleeve fixing seat is provided with a guide groove, and the boss is assembled with the guide groove.

[0013] Based on the above technical solution, furthermore, the magnetic rod and the magnetic rod sleeve fixing base are concentric.

[0014] Compared with the prior art, the beneficial effects of this utility model are specifically reflected in:

[0015] This invention features a magnetic rod sleeve with ribs, bosses, and notches, allowing it to work in conjunction with the magnetic rod during operation. Simultaneously, the structure of the drive assembly, magnetic rod support plate, and magnetic rod fixing plate drives the movement of the magnetic rod, and the magnetic attraction function controls the magnetic column for nucleic acid purification. The entire process prevents the magnetic rod sleeve from shifting or falling off, improving the assembly stability between the various structures and the efficiency of the device's testing. Attached Figure Description

[0016] Figure 1 This is a schematic diagram of the nucleic acid extraction device of this utility model;

[0017] Figure 2 This is a schematic diagram of the structure of the magnetic rod sleeve of this utility model;

[0018] Figure 3 This is a schematic diagram of the structure of the magnetic rod sleeve and magnetic rod sleeve fixing base before operation of this utility model;

[0019] Figure 4 This is a schematic diagram of the structure of the magnetic rod sleeve and magnetic rod sleeve fixing base in operation.

[0020] Figure 5 This is a schematic diagram of the structure of the magnetic rod sleeve and magnetic rod sleeve fixing base after they are in operation.

[0021] Figure label:

[0022] 1. Magnetic rod sleeve; 2. Magnetic rod sleeve fixing base; 3. Magnetic rod; 4. Magnetic rod base support plate; 5. Magnetic rod fixing plate; 6. Lead screw nut; 7. Motor lead screw; 8. Drive motor; 101. Rib; 102. Boss; 103. Notch; 201. Guide groove; 202. Lead angle. Detailed Implementation

[0023] To make the objectives and technical solutions of this utility model clearer, the technical solutions of this utility model will be clearly and completely described below in conjunction with the embodiments.

[0024] Example

[0025] Combination Figures 1-5 As shown, this embodiment provides a nucleic acid extraction device, which includes a magnetic rod sleeve 1, a magnetic rod assembly, a magnetic rod support assembly, and a driving assembly; wherein, the magnetic rod assembly includes a magnetic rod sleeve fixing seat 2 and a magnetic rod 3, the magnetic rod sleeve fixing seat 2 is movably sleeved on the magnetic rod 3 and keeps concentric with the magnetic rod 3, and the driving assembly drives the magnetic rod support assembly to move up and down, and the magnetic rod support assembly drives the magnetic rod 3 to move up and down; the magnetic rod sleeve 1 is assembled with the magnetic rod sleeve fixing seat 2.

[0026] In this embodiment, the end of the magnetic rod sleeve 1 is provided with multiple evenly distributed ribs 101, multiple evenly distributed bosses 102, and multiple evenly distributed notches 103. The ribs 101 are distributed on the outer surface of the magnetic rod sleeve 1, the bosses 102 are distributed on the inner surface of the magnetic rod sleeve 1, and the notches 103 are formed at the upper edge of the magnetic rod sleeve 1. It should be noted that the number of ribs 101, bosses 102, and notches 103 can be set according to actual needs. By reasonably arranging the ribs 101, the position of the magnetic rod sleeve 1 in the reagent strip hole (not shown in the figure) can be restricted, keeping it in a centered state, facilitating its cooperation with the magnetic rod sleeve fixing seat 2. At the same time, it can also evenly distribute stress, preventing problems such as twisting or collapse of the component due to localized stress concentration or temperature changes.

[0027] In this embodiment, the magnetic rod sleeve fixing base 2 is provided with a guide groove 201 and an inlet angle. The guide groove 201 is used to dock with the magnetic rod sleeve 1, and the guide angle 202 is set to facilitate accurate guidance of the magnetic rod sleeve fixing base 2 to connect with the magnetic rod sleeve 1.

[0028] In this embodiment, the magnetic rod support assembly includes a magnetic rod base support plate 4 and a magnetic rod fixing plate 5. The magnetic rod base support plate 4 is located below the magnetic rod fixing plate 5, and the magnetic rod base support plate 4 and the magnetic rod fixing plate 5 are not directly connected. Multiple magnetic rods 3 are mounted on the magnetic rod fixing plate 5. Figure 1 As shown, eight magnetic rods 3 are mounted on the magnetic rod fixing plate 5. Each magnetic rod 3 is fitted with a magnetic rod sleeve fixing seat 2, which is mounted on the magnetic rod seat support plate 4. Specifically, the magnetic rod 3 passes through the magnetic rod seat support plate 4 and the magnetic rod sleeve fixing seat 2 and can move up and down. It should be noted that the relative positions of the magnetic rod 3 and the magnetic rod sleeve fixing seat 2 are concentrically arranged vertically.

[0029] In this embodiment, the drive assembly includes a drive motor 8, a motor lead screw 7, and a lead screw nut 6. The power output shaft of the drive motor 8 is connected to the motor lead screw 7, and the motor lead screw 7 passes through the lead screw nut 6 and extends to connect with the magnetic rod seat support plate 4.

[0030] Furthermore, the magnetic rod sleeve 1 is placed in the matching reagent strip (not shown in the figure). When the magnetic rod 3 moves to the top of the magnetic rod sleeve 1, the drive motor 8 is started to work in the forward direction, which in turn causes the lead screw motor to start rotating. The magnetic rod seat support plate 4 starts to move downward under the drive of the lead screw nut 6 until one or more magnetic rod sleeve fixing seats 2 are engaged with the magnetic rod sleeve 1.

[0031] Furthermore, the notch 103 structure at the upper end of the magnetic rod sleeve 1 makes the top of the magnetic rod sleeve 1 elastic. It can be understood that when the magnetic rod sleeve fixing seat 2 is slowly inserted into the magnetic rod sleeve 1, the top of the magnetic rod sleeve 1 is opened by force until the protrusion 102 inside the magnetic rod sleeve 1 is located in the guide groove 201 on the magnetic rod sleeve fixing seat 2, at which point the magnetic rod sleeve 1 and the magnetic rod sleeve fixing seat 2 are fully engaged and achieve a tight fit.

[0032] In this embodiment, the magnetic rod fixing plate 5 can move up and down under the drive of an external force (not shown in the figure), which in turn causes the magnetic rod 3 to move up and down. When magnetic beads need to be adsorbed in the extraction process, the magnetic rod 3 will first move down to the bottom position of the magnetic rod sleeve 1, and then the magnetic rod 3 and the magnetic rod sleeve 1 will move down into the liquid containing the magnetic beads at the same time to complete the adsorption of the magnetic beads. It can be understood that the magnetic beads will be adsorbed at the bottom of the magnetic rod sleeve 1. When the magnetic rod 3 moves up and detaches from the magnetic rod sleeve 1, the magnetic beads lose their magnetic adsorption and will fall off the magnetic rod sleeve 1 back into the liquid, thereby achieving the purpose of controlling the magnetic beads to purify nucleic acids.

[0033] Furthermore, after the magnetic rod sleeve 1 completes the extraction process, the magnetic rod fixing plate 5 moves slowly downward under the drive of external force, and the magnetic rod 3 also moves downward until it is attached to the bottom of the magnetic rod sleeve 1 and then continues to move downward. At this time, the magnetic rod sleeve 1 is subjected to a downward force and detaches from the magnetic rod sleeve fixing seat 2, falling into the matching reagent strip (not shown in the figure), thus realizing the detachment of the magnetic rod sleeve 1.

[0034] The above are merely embodiments of this utility model, described in a relatively specific and detailed manner, but they should not be construed as limiting the scope of this utility model patent. It should be noted that those skilled in the art can make various modifications and improvements without departing from the concept of this utility model, and these all fall within the protection scope of this utility model.

Claims

1. A nucleic acid extraction device, characterized by, The magnetic rod sleeve, the magnetic rod assembly, the magnetic rod supporting assembly and the driving assembly are included. The magnetic rod sleeve fixing seat is movably sleeved on the magnetic rod. The driving assembly drives the magnetic rod supporting assembly to move up and down, and the magnetic rod supporting assembly drives the magnetic rod to move up and down. The magnetic rod sleeve is assembled with the magnetic rod.

2. The nucleic acid extraction device according to claim 1, wherein The magnetic rod supporting assembly includes a magnetic rod seat supporting plate and a magnetic rod fixing plate, and the magnetic rod seat supporting plate is arranged below the magnetic rod fixing plate.

3. The nucleic acid extraction device of claim 2, wherein The driving assembly includes a driving motor, a motor lead screw and a lead screw nut, the power output shaft of the driving motor is connected with the motor lead screw, the motor lead screw passes through the lead screw nut and extends to be connected with the magnetic rod seat supporting plate.

4. The nucleic acid extraction device of claim 1, wherein The magnetic rod sleeve is provided with a rib and an opening, the rib is distributed on the outer surface of the magnetic rod sleeve, and the opening is arranged on the upper edge of the magnetic rod sleeve.

5. The nucleic acid extraction device of claim 1, wherein The magnetic rod sleeve fixing seat is provided with a guide-in angle.

6. The nucleic acid extraction device of claim 1, wherein The inner surface of the upper end of the magnetic rod sleeve is further provided with a boss, the magnetic rod sleeve fixing seat is provided with a guide groove, and the boss is assembled with the guide groove.

7. The nucleic acid extraction device of claim 1, wherein The magnetic rod is concentric with the magnetic rod sleeve fixing seat.

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