A portable enteric bacteria liquid preparation device

Abstract

The utility model discloses a portable intestinal tract bacteria liquid preparation device, including incubator 34, detachable culture bottle is installed to the culture box, and the culture bottle includes the primary culture bottle and secondary culture bottle who arranges in proper order, and the primary culture bottle and secondary culture bottle below corresponded to be provided with magnetic stirrer, and the primary culture bottle and secondary culture bottle are provided with the magnetic bead who cooperates with magnetic stirrer, and the primary culture bottle is equipped with the primary liquid inlet and primary liquid outlet for injecting culture medium, and the secondary culture bottle is equipped with secondary liquid inlet, nutrient liquid filling mouth and secondary liquid outlet, and the primary liquid outlet and secondary liquid outlet are provided with filter system, and the secondary liquid inlet is connected with the primary liquid outlet through the infusion pipe who is connected with the peristaltic pump. The utility model discloses convenient to carry use, and it is convenient to assemble and replace, avoids cross contamination, reduces staff operation discomfort, reduces manual contact, improves processing efficiency and standardization degree simultaneously, reduces the probability of exogenous pollution.

Description

Technical Field

[0001] This utility model relates to the field of medical equipment technology, specifically a portable intestinal bacterial culture preparation device. Background Technology

[0002] The gut microbiota is a balanced ecosystem that plays a vital role in maintaining the health of humans and animals. Dysregulation of the gut microbiota is a significant factor in inducing various chronic diseases. Recent domestic and international research has found that fecal microbiota transplantation (FMT), which uses gut microbiota from healthy individuals to restore the balance of the gut microbiota in patients with chronic diseases and rebuild a normally structured gut microecology, can promote the recovery of microbiota function, thereby achieving therapeutic effects for both intestinal and extraintestinal diseases. To date, there have been clinical reports both domestically and internationally of successful treatments or alleviating gastrointestinal-related diseases such as Clostridium difficile infection and inflammatory bowel disease using FMT. Although FMT has significant clinical efficacy, its widespread application in the medical field still faces many challenges. For example, the core step in FMT—fecal microbiota processing—currently relies heavily on manual stirring and filtration. This process involves direct contact with the external environment, easily leading to exogenous infections, causing discomfort for staff, and is inconvenient to carry. Utility Model Content

[0003] The purpose of this invention is to provide a portable intestinal bacterial culture preparation device to solve the problems mentioned in the background art.

[0004] To achieve the above objectives, this utility model provides the following technical solution: a portable intestinal bacterial culture preparation device, comprising an incubator, wherein a detachable culture bottle is snapped into the incubator, the culture bottle comprising a primary culture bottle and a secondary culture bottle arranged in sequence, a magnetic stirrer correspondingly disposed below the primary culture bottle and the secondary culture bottle, and magnetic beads cooperating with the magnetic stirrer disposed inside the primary culture bottle and the secondary culture bottle, the primary culture bottle having a primary inlet and a primary outlet for injecting culture medium, the secondary culture bottle having a secondary inlet, a nutrient solution inlet, and a secondary outlet, the primary outlet and the secondary outlet being provided with a filtration system, and the secondary inlet being connected to the primary outlet via a delivery tube connected to a peristaltic pump.

[0005] Furthermore, the primary culture flask and the secondary culture flask are sealed structures.

[0006] Furthermore, the secondary culture flask includes multiple culture flasks connected in series, and the nutrient solution inlet is located on any one of the culture flasks.

[0007] Furthermore, the device also includes a heater, which is disposed on the infusion tube of the secondary outlet.

[0008] Furthermore, the incubator is equipped with a controller.

[0009] Furthermore, the device also includes a nutrient solution tank, and the nutrient solution filling port is connected to the nutrient solution tank via an infusion pipe connected to a peristaltic pump.

[0010] Furthermore, the filtration system includes a filter membrane.

[0011] Furthermore, the incubator is provided with a slot for locking the culture flask, and the slot is adapted to the shape of the culture flask.

[0012] Compared with the prior art, the beneficial effects of this utility model are as follows: This utility model compactly integrates primary culture bottles, secondary culture bottles, and supporting components such as stirrers and filtration systems into an incubator, achieving structural integration and miniaturization, making it convenient to carry and use, and easy to assemble and replace, avoiding cross-contamination. Through the sealed design of the primary and secondary culture bottles, combined with the infusion tube transmission, a fully closed operation is achieved from culture medium injection, bacterial culture to final output. The use of a magnetic stirrer and magnetic beads to achieve automated stirring makes the bacterial solution more uniformly mixed. The filtration system removes large particles from the bacterial solution and filters and purifies the bacterial solution, improving the activity and stability of the bacterial solution, reducing operator discomfort, reducing manual contact, improving processing efficiency and standardization, and reducing the probability of external contamination. Attached Figure Description

[0013] Figure 1 This is a schematic diagram of the portable intestinal bacterial culture preparation device of this utility model;

[0014] Figure 2 This is a schematic diagram of the incubator in the portable intestinal bacterial culture preparation device of this utility model;

[0015] In the diagram, 100-incubator, 101-card slot, 102-controller, 1-primary culture flask, 11-primary inlet, 12-primary outlet, 21-secondary inlet, 22-nutrient solution filling port, 23-secondary outlet, 3-magnetic stirrer, 4-magnetic bead, 5-nutrient solution tank, 6-heater, 7-peristaltic pump, 8-transfer pump. Detailed Implementation

[0016] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the protection scope of the present utility model.

[0017] like Figure 1 and Figure 2As shown, this embodiment provides a portable intestinal bacterial culture preparation device, including an incubator 100. A detachable culture bottle and a nutrient solution tank 5 are snapped together inside the incubator 100. For example, a slot 101 for securing the culture bottle can be provided inside the incubator 100. The slot 101 is adapted to the shape of the culture bottle to secure it. Elastic ropes can also be added to tighten the culture bottle, preventing it from shaking or falling, facilitating assembly. Furthermore, it can be disassembled and replaced after each patient's use, avoiding cross-contamination. The culture bottle includes a primary culture bottle 1 and a secondary culture bottle 2 arranged sequentially. The primary culture bottle 1 is provided with a primary inlet 11 and a primary outlet 12 for injecting culture medium. The primary culture bottle 2 is equipped with a secondary inlet 21, a nutrient solution filling port 22, and a secondary outlet 23. Both the primary culture bottle 1 and the secondary culture bottle 2 are sealed structures. For example, silicone stoppers are used to seal the mouths of the primary culture bottle 1 and the secondary culture bottle 2. The silicone stoppers are inserted through an infusion set head to form the primary inlet 11, primary outlet 12, secondary inlet 21, nutrient solution filling port 22, and secondary outlet 23. The secondary inlet 21 is connected to the primary outlet 12 through an infusion tube connected to a peristaltic pump. The nutrient solution filling port 22 is connected to the nutrient solution tank 5 through an infusion tube connected to a peristaltic pump. The nutrient solution tank 5 can hold probiotics, dietary fiber, and other nutritional products. The cooperation between the nutrient solution tank 5 and the peristaltic pump enables quantitative addition of nutrients. Note that, to accommodate the different bacterial culture volume requirements of various patients, the sealed design of the primary and secondary culture bottles, combined with infusion tubing for efficient delivery, and driven by a peristaltic pump 7, achieves a completely closed process for fecal microbial treatment, from culture medium injection, primary culture, secondary amplification to final bacterial culture output. This avoids direct contact with the external environment, reducing the risk of exogenous infection. Magnetic stirrers 3 are installed below the primary and secondary culture bottles 1 and 2, respectively. Magnetic beads 4 are installed inside the primary and secondary culture bottles 1 and 2 to cooperate with the magnetic stirrers 3, enabling automated stirring, reducing direct contact between staff and fecal samples, effectively reducing operational discomfort, and improving processing efficiency. The number of secondary culture bottles 2 can be adjusted according to actual needs. The secondary culture flask 2 includes multiple culture flasks connected in series. The nutrient solution inlet 22 is located on any one of the culture flasks, for example, 1 to 3 can be set. In this embodiment, 2 secondary culture flasks 2 are set, and the nutrient solution inlet 22 is located on the second culture flask. The primary outlet and the secondary outlet are equipped with a filtration system. The filtration system of the primary outlet 12 is used to filter large particles and impurities in the primary culture liquid, and can use a standard sieve of 80 to 100 mesh. The filtration system of the secondary outlet of the first secondary culture flask uses a standard sieve of 250 to 300 mesh. The filtration system of the secondary outlet of the second secondary culture flask is used to filter and purify the final bacterial liquid, and can use a filter membrane with a pore size of 15 to 25 μm.A heater 6 can be installed on the infusion tube at the secondary outlet 23 to heat the bacterial solution to 37℃~40℃ for subsequent use. The heater 6 can be an infusion thermostat, using heating elements as a heat source, and has a power socket or charging port. It uses a clip-type opening mechanism with an infusion tube insertion hole. The infusion tube is inserted into the insertion hole, and power is applied to heat the tube via the heating elements, thereby heating the bacterial solution inside. Infusion thermostats are commercially available and will not be described in detail here. The incubator 100 compactly integrates the primary culture bottle, secondary culture bottle, and supporting components such as a stirrer, filtration system, and heater into the incubator, achieving structural integration and miniaturization for easy portability and use. Alternatively, the infusion tube at the secondary outlet 23 can be connected to a peristaltic pump machine. The peristaltic pump machine has heating and speed regulation functions, allowing the prepared bacterial solution to be infused into the human intestine. The peristaltic pump machine is a commercially available device and will not be described in detail here. The incubator 100 is equipped with a controller 102, which is electrically connected to a magnetic stirrer, a peristaltic pump, and a heater. The controller 102 controls the stirring speed of the magnetic stirrer, the start and stop and the flow rate of the peristaltic pump, and adds nutrient solution quantitatively according to a preset program. It also controls the heating temperature of the heater, thereby realizing automated culture control, improving the standardization of fecal microbial treatment, and ensuring the stability and consistency of the microbial solution quality.

[0018] In operation, the pretreated fecal sample and culture medium are injected into the primary inlet 11. The controller 102 activates the magnetic stirrer 3, which drives the magnetic beads 4 to stir the culture medium, completing the initial activation of fecal bacteria to form a primary bacterial solution. After the primary bacterial solution is filtered through an 80-100 mesh standard sieve to remove large particles and impurities, it is pumped into the secondary inlet 21 of the first secondary culture bottle 2 by the peristaltic pump 7. The controller 102 activates the magnetic stirrer 3, which drives the magnetic beads 4 to stir the primary bacterial solution. After filtration through a 250-300 mesh standard sieve, the primary bacterial solution is further stirred. After standard filtration, the solution is pumped into the secondary inlet 21 of the second secondary culture bottle 2 by peristaltic pump 7. The controller adds nutrient solution quantitatively from the nutrient solution tank 5 according to a preset program, for example, by using a transfer pump 8. The amplification culture is completed under stirring to form the final bacterial solution. The final bacterial solution is filtered through a filter membrane with a pore size of 15~25μm to form a purified bacterial solution, which is output from the secondary outlet 23. The solution is then heated to 37℃~40℃ by a heater and pumped into the human intestine by peristaltic pump 7, or by a peristaltic pump machine.

[0019] This invention compactly integrates primary culture flasks, secondary culture flasks, and supporting components such as stirrers and filtration systems into an incubator, achieving structural integration and miniaturization. This makes it easy to carry and use, assemble and replace components, and avoids cross-contamination. The sealed design of the primary and secondary culture flasks, combined with infusion tubing, enables a completely closed operation from culture medium injection and bacterial culture to final output. Automated stirring using a magnetic stirrer and magnetic beads ensures more uniform mixing of the bacterial solution. The filtration system removes large particles and purifies the bacterial solution, improving its activity and stability, reducing operator discomfort, minimizing manual contact, and simultaneously increasing processing efficiency and standardization while reducing the probability of external contamination.

[0020] Although the present invention has been described in detail with reference to the foregoing embodiments, those skilled in the art can still modify the technical solutions described in the foregoing embodiments or make equivalent substitutions for some of the technical features. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of the present invention should be included within the protection scope of the present invention.

Claims

1. A portable intestinal bacterial culture preparation device, characterized in that: The device includes an incubator, within which a detachable culture flask is snap-fitted. The culture flask comprises a primary culture flask and a secondary culture flask arranged sequentially. A magnetic stirrer is correspondingly disposed below each of the primary and secondary culture flasks. Magnetic beads that cooperate with the magnetic stirrers are disposed inside each of the primary and secondary culture flasks. The primary culture flask is provided with a primary inlet and a primary outlet for injecting culture medium. The secondary culture flask is provided with a secondary inlet, a nutrient solution inlet, and a secondary outlet. A filtration system is provided at the primary outlet and the secondary outlet. The secondary inlet is connected to the primary outlet via a delivery tube connected to a peristaltic pump.

2. The portable intestinal bacterial culture preparation device according to claim 1, characterized in that: The primary and secondary culture flasks are sealed.

3. The portable intestinal bacterial culture preparation device according to claim 1, characterized in that: The secondary culture flask includes multiple culture flasks connected in series, and the nutrient solution inlet is located on any one of the culture flasks.

4. The portable intestinal bacterial culture preparation device according to claim 1, characterized in that: The device also includes a heater, which is disposed on the infusion tube of the secondary outlet.

5. The portable intestinal bacterial culture preparation device according to claim 1, characterized in that: The incubator is equipped with a controller.

6. The portable intestinal bacterial culture preparation device according to claim 1, characterized in that: The device also includes a nutrient solution tank, and the nutrient solution filling port is connected to the nutrient solution tank via an infusion pipe connected to a peristaltic pump.

7. The portable intestinal bacterial culture preparation device according to claim 1, characterized in that: The filtration system includes a filter membrane.

8. The portable intestinal bacterial culture preparation device according to claim 1, characterized in that: The incubator is equipped with a slot for locking the culture flask, and the slot is adapted to the shape of the culture flask.

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