A device for breaking the cell wall by oscillation and an apparatus

Abstract

The utility model discloses a kind of oscillation wall breaking devices and equipment, it is related to the technical field of nucleic acid extraction cell wall breaking, device includes oscillation base, support assembly and oscillation component;Oscillation component includes sample tube rack, driving mechanism and oscillation control mechanism;Support assembly is installed on oscillation base, oscillation control mechanism is installed on support assembly, driving mechanism is installed on support assembly, sample tube rack is all installed on oscillation control mechanism, and driving mechanism drives sample tube rack to vibrate in oscillation control mechanism.Oscillation wall breaking device and equipment are included in the equipment.Oscillation wall breaking device is used to break cell wall by high-frequency oscillation mode, improve the stability and efficiency of fungus and bacteria wall breaking.

Description

Technical Field

[0001] This utility model relates to the technical field of cell wall disruption for nucleic acid extraction, and in particular to a shaking cell wall disruption device and equipment. Background Technology

[0002] Nucleic acid extraction is a fundamental technique in modern molecular biology research. Cell disruption is a crucial pretreatment step that directly affects the sensitivity and accuracy of subsequent detection. Efficient cell disruption technology can fully release intracellular nucleic acids, allowing nucleic acids (such as DNA and RNA) and other biomolecules that were originally encapsulated within the cell to be released into solution, creating conditions for subsequent nucleic acid extraction and purification.

[0003] In existing technologies, high-frequency vibration cell disruption uses mechanical shearing force to rupture cell walls, offering advantages such as ease of operation and high efficiency. However, it still suffers from low vibration energy transfer efficiency, resulting in poor adaptability to different cell types (such as Gram-positive bacteria and plant cells). Traditional vibration container designs also suffer from small sample processing capacity and unstable disruption efficiency. Traditional ultrasonic cell disruption utilizes cavitation effects to decompose cell walls, which involves complex designs, requires setting interval times, involves multiple operation steps, and has high maintenance costs. It is also prone to causing a sharp temperature rise, and in large-scale applications, uneven acoustic energy distribution affects the consistency of cell disruption.

[0004] Therefore, it is necessary to design a simple, stable and efficient cell-wall breaking structure. Utility Model Content

[0005] To address the existing problems, this utility model provides a vibrating cell wall breaking device and equipment.

[0006] To achieve the objectives of this utility model, the technical solution adopted is as follows:

[0007] A vibration cell wall breaking device includes a vibration base, a support assembly, and a vibration assembly; the vibration assembly includes a sample tube rack, a drive mechanism, and a vibration control mechanism; the support assembly is mounted on the vibration base, the vibration control mechanism is mounted on the support assembly, the drive mechanism is mounted on the support assembly, and the sample tube rack is mounted on the vibration control mechanism, wherein the drive mechanism drives the sample tube rack to vibrate within the vibration control mechanism.

[0008] Based on the above technical solution, the support component further includes an oscillating side plate, an oscillating top plate, and a rubber column. The bottom of the rubber column is installed on the oscillating base, the top of the rubber column is connected to the oscillating top plate, the oscillating side plate is installed on the rubber column, and the oscillating component is installed on the oscillating top plate.

[0009] Furthermore, based on the above technical solution, the upper oscillation plate is equipped with a guide rail.

[0010] Based on the above technical solution, the oscillation control mechanism further includes a photoelectric baffle and a photoelectric switch, with the photoelectric switch installed on one side of the oscillation upper plate and a slider at the bottom of the photoelectric baffle, which cooperates with the slide rail.

[0011] Furthermore, based on the above technical solution, a sample tube rack is installed above the photoelectric baffle, and a driving mechanism is provided below the photoelectric baffle.

[0012] Based on the above technical solution, the drive mechanism is further installed below the oscillating upper plate, and the power output shaft of the drive mechanism is connected to the lower part of the photoelectric baffle.

[0013] Furthermore, based on the above technical solution, the drive mechanism is a DC brushless motor.

[0014] A oscillating cell-wall breaking device includes an oscillating cell-wall breaking apparatus and an oscillating cell-wall breaking tube, wherein the oscillating cell-wall breaking tube is mounted on the sample tube rack of the oscillating cell-wall breaking apparatus.

[0015] Based on the above technical solution, the vibrating wall-breaking tube further includes a sealing membrane, a tube cap, and a tube body. The tube cap is installed at the upper end of the tube body, and the sealing membrane is provided at the upper end of the tube cap.

[0016] Based on the above technical solution, the upper end of the tube body is further provided with a groove, which is used to fix it on the sample tube rack.

[0017] Compared with the prior art, the beneficial effects of this utility model are specifically reflected in:

[0018] (1) This invention uses high-frequency oscillation to disrupt cell walls. It employs a shock-absorbing design to eliminate resonance effects and effectively control noise. Pre-placed glass beads and a sealed cap prevent contamination from glass beads scattering. The glass beads disrupt the cell walls through repeated friction and compression with the cells. This high-frequency oscillation method reduces nucleic acid damage compared to traditional cell disruption methods. The vibration frequency is 3000 r / min, driven by a DC brushless motor, improving the stability and efficiency of cell disruption for fungi and bacteria.

[0019] (2) The oscillating cell wall breaking tube provided by this utility model can quantitatively pre-place glass beads and drive the sample tube rack through a DC brushless motor, which can more thoroughly break the cell walls of fungi and bacteria, is efficient and stable, effectively controls noise, and is economical, easy to operate and maintain. It is well sealed and pollution-free, and does a good job of breaking the cell walls for subsequent nucleic acid extraction of the instrument.

[0020] (3) This utility model ensures that the puncture sampling gun tip can quickly pierce through the sealing film and take the sample that has been broken up to the lysis site through the sealing film. Attached Figure Description

[0021] Figure 1This is a schematic diagram of the overall structure of the vibration cell-breaking device provided by this utility model.

[0022] Figure 2 This is a structural schematic diagram of the vibrating cell wall breaking device provided by this utility model.

[0023] Figure 3 This is a top view of the vibrating cell-wall breaking device provided by this utility model.

[0024] Figure 4 This is a schematic diagram of the structure of the vibrating wall-breaking tube provided by this utility model.

[0025] Figure 5 for Figure 4 Exploded view.

[0026] Figure 6 for Figure 4 Cross-sectional view.

[0027] Reference numerals: 12, Vibrating cell wall breaking device; 1201, Vibrating base; 1202, Vibrating side plate; 1203, Vibrating upper plate; 1204, Sample tube rack; 1205, Drive mechanism; 1206, Photoelectric switch; 1207, Rubber column; 1208, Photoelectric baffle; 1000, Vibrating cell wall breaking tube; 1001, Sealing film; 1002, Tube cap; 1003, Tube body; 1004, Groove. Detailed Implementation

[0028] The present invention will be further described and illustrated below with reference to the accompanying drawings and specific embodiments. The technical features of each embodiment of the present invention can be combined accordingly, provided that there is no mutual conflict.

[0029] To make the above-mentioned objectives, features, and advantages of this utility model more apparent and understandable, the specific embodiments of this utility model will be described in detail below with reference to the accompanying drawings. Many specific details are set forth in the following description to provide a full understanding of this utility model. However, this utility model can be implemented in many other ways different from those described herein, and those skilled in the art can make similar improvements without departing from the spirit of this utility model. Therefore, this utility model is not limited to the specific embodiments disclosed below. Technical features in various embodiments of this utility model can be combined appropriately without conflict.

[0030] In the description of this utility model, it should be understood that when an element is considered to be "connected" to another element, it can be directly connected to the other element or indirectly connected, i.e., there is an intermediate element. Conversely, when an element is said to be "directly" connected to another element, there is no intermediate element.

[0031] Example

[0032] Combination Figure 1 As shown, this embodiment provides a vibration-damping device 12, which includes a vibration base 1201, a support assembly, and a vibration assembly. The vibration assembly includes a sample tube rack 1204, a drive mechanism 1205, and a vibration control mechanism. The support assembly is mounted on the vibration base 1201, the vibration control mechanism is mounted on the support assembly, the drive mechanism 1205 is mounted on the support assembly, and the sample tube rack 1204 is mounted on the vibration control mechanism. The drive mechanism 1205 drives the sample tube rack 1204 to vibrate within the vibration control mechanism.

[0033] In this embodiment, the support assembly includes an oscillating side plate 1202, an oscillating upper plate 1203, and a rubber column 1207. The bottom of the rubber column 1207 is mounted on the oscillating base 1201, and the top of the rubber column 1207 is connected to the oscillating upper plate 1203. The oscillating side plate 1202 is mounted on the rubber column 1207, the oscillating assembly is mounted on the oscillating upper plate 1203, and the driving mechanism 1205 is mounted on the oscillating upper plate 1203.

[0034] Reference Figure 1 Preferably, two vibration side plates 1202 are provided, and at least two rubber columns 1207 are provided. To improve support stability, four rubber columns 1207 are preferably provided, with two rubber columns 1207 forming a group, respectively located on different sides of the vibration base 1201. Each group of rubber columns 1207 is equipped with one vibration side plate 1202. The rubber columns 1207 are used for vibration damping design, eliminating resonance effects and effectively controlling noise.

[0035] In this embodiment, a guide rail (not shown in the figure) is mounted on the upper oscillating plate 1203. The oscillation control mechanism includes a photoelectric baffle 1208 and a photoelectric switch 1206. The photoelectric switch 1206 is installed on one side of the upper oscillating plate 1203. A slider (not shown in the figure) is provided at the bottom of the photoelectric baffle 1208. The slider cooperates with the guide rail, and the slider drives the photoelectric baffle 1208 to move left and right on the guide rail. That is, the photoelectric baffle 1208 and the photoelectric switch 1206 cooperate to perform position control. Specifically, the photoelectric baffle 1208 moves horizontally left and right under the action of the drive mechanism 1205. When the thin strip on the left side of the photoelectric baffle 1208 moves to the center position of the photoelectric switch 1206, the entire oscillation control mechanism stops moving and is in a zero position.

[0036] Furthermore, a sample tube rack 1204 is installed above the photoelectric baffle 1208, and a drive mechanism 1205 is located below the photoelectric baffle 1208. Specifically, the drive mechanism 1205 is installed below the oscillating upper plate 1203, and the power output shaft of the drive mechanism 1205 is connected to the lower part of the photoelectric baffle 1208 through an eccentric cam and a bearing (not shown in the figure). The drive mechanism 1205 is an oscillating motor, which is a DC brushless motor. When the oscillating motor is started, its power output shaft speed is set to 3000 r / min, and its vibration will drive the sample tube rack 1204 to move horizontally left and right.

[0037] In some other embodiments, combined Figures 2-6 As shown, this embodiment provides a oscillating cell-wall breaking device, which includes an oscillating cell-wall breaking device 12 and an oscillating cell-wall breaking tube 1000 installed on a sample tube rack 1204 of the oscillating cell-wall breaking device 12.

[0038] In this embodiment, the vibrating cell-wall breaking tube 1000 includes a sealing membrane 1001, a tube cap 1002, and a tube body 1003. The tube cap 1002 is installed at the upper end of the tube body 1003, and the sealing membrane 1001 is provided at the upper end of the tube cap 1002. The tube body 1003 can pre-quantitatively contain glass beads for storing samples such as fungi or bacteria. The tube cap 1002 and the sealing membrane 1001 are then placed at the upper end of the tube body 1003. The tube cap 1002 achieves a contamination-preventing effect by tightening and sealing, while the sealing membrane 1001 provides a seal. Together, they effectively prevent glass beads from scattering and causing contamination. It should be noted that the sealing membrane 1001 also facilitates rapid puncture of the sealing membrane by the puncture sampling nozzle tip for sampling, thereby enabling the extraction of the lysed sample from the cell-wall breaking site.

[0039] Furthermore, the upper end of the tube body 1003 is provided with a groove 1004 structure, which facilitates fixing on the sample tube rack 1204 of the vibrating cell wall breaking device 12. Preferably, the sample tube rack 1204 is provided with a protrusion that fits perfectly with the groove 1004, which can securely fix it. It should be noted that the structure that mates with the groove 1004 is not specifically limited and can be set according to the actual situation, as long as the fixing effect is achieved. Further details are not provided here.

[0040] The above are merely embodiments of this utility model, described in a relatively specific and detailed manner, but they should not be construed as limiting the scope of this utility model patent. It should be noted that those skilled in the art can make various modifications and improvements without departing from the concept of this utility model, and these all fall within the protection scope of this utility model.

Claims

1. A vibrating cell wall breaking device, characterized in that, It includes an oscillation base, a support assembly, and an oscillation assembly; the oscillation assembly includes a sample tube rack, a drive mechanism, and an oscillation control mechanism; the support assembly is mounted on the oscillation base, the oscillation control mechanism is mounted on the support assembly, the drive mechanism is mounted on the support assembly, and the sample tube rack is mounted on the oscillation control mechanism, with the drive mechanism driving the sample tube rack to vibrate within the oscillation control mechanism.

2. The vibrating cell-wall breaking device according to claim 1, characterized in that, The support assembly includes an oscillating side plate, an oscillating top plate, and a rubber column. The bottom of the rubber column is mounted on the oscillating base, and the top of the rubber column is connected to the oscillating top plate. The oscillating side plate is mounted on the rubber column, and the oscillating assembly is mounted on the oscillating top plate.

3. The vibrating cell-wall breaking device according to claim 2, characterized in that, The upper plate of the oscillating device is equipped with guide rails.

4. The vibrating cell-wall breaking device according to claim 3, characterized in that, The oscillation control mechanism includes a photoelectric baffle and a photoelectric switch. The photoelectric switch is installed on one side of the upper plate of the oscillation, and a slider is provided at the bottom of the photoelectric baffle. The slider cooperates with the slide rail.

5. The vibrating cell-wall breaking device according to claim 4, characterized in that, A sample tube rack is installed above the photoelectric baffle, and a driving mechanism is provided below the photoelectric baffle.

6. The vibrating cell-wall breaking device according to claim 5, characterized in that, The drive mechanism is installed below the vibrating upper plate, and the power output shaft of the drive mechanism is connected to the lower part of the photoelectric baffle.

7. The vibrating cell-wall breaking device according to claim 1, characterized in that, The drive mechanism is a DC brushless motor.

8. A vibrating cell-wall breaking device, characterized in that, The device includes a oscillating cell-breaking device as described in any one of claims 1-7, and further includes an oscillating cell-breaking tube, wherein the oscillating cell-breaking tube is mounted on the sample tube rack of the oscillating cell-breaking device.

9. The vibrating cell-wall breaking device according to claim 8, characterized in that, The vibrating cell wall breaking tube includes a sealing membrane, a tube cap, and a tube body. The tube cap is installed at the upper end of the tube body, and the sealing membrane is provided at the upper end of the tube cap.

10. The vibrating cell-wall breaking device according to claim 9, characterized in that, The upper end of the tube is also provided with a groove, which is used to fix it to the sample tube rack.

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