A sampling device for flow cytometry
By introducing locking and adjustable limiting components into the flow cytometry sampling device, the problems of wedge block detachment and inability to adjust the limiting ring were solved, achieving accuracy of sampling position and stability of the container, and improving the adaptability and operational efficiency of the device.
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- SHENZHEN CCIA NEW DRUG TESTING CO LTD
- Filing Date
- 2025-08-22
- Publication Date
- 2026-07-21
AI Technical Summary
Existing flow cytometry sampling devices are prone to detachment of the wedge block from the wedge groove due to vibration during rotation, affecting the accuracy of the sampling position. Furthermore, the limiting ring cannot be adjusted, resulting in poor adaptability and causing the container to shake or fail to be placed.
The device employs a locking assembly and an adjustable limit assembly. The locking assembly uses a compression spring made of high-strength alloy spring steel and a positioning bead to stably lock the wedge block and prevent it from shifting. The adjustable limit assembly uses a telescopic spring and a damping plate to adjust the limit distance to accommodate containers of different sizes.
It improves the accuracy and stability of the sampling location, prevents container shaking, enhances the device's adaptability to containers of different sizes, and ensures the efficiency and stability of the sampling process.
Smart Images
Figure CN224535499U_ABST
Abstract
Description
Technical Field
[0001] This utility model relates to the field of cell detection technology, and in particular to a sampling device for flow cytometry detection. Background Technology
[0002] Cell detection refers to the process of observing, analyzing, and measuring the morphology, physiological function, proliferation and differentiation, genetic material, and biomarkers of cells using tools such as microscopes and flow cytometers, employing biological and chemical techniques. Its purpose is to understand the state of cells, detect abnormal changes, and provide a basis for disease diagnosis, drug development, and life science research. It encompasses multiple aspects, including the study of normal cell characteristics and the identification of diseased cells. Flow cytometry requires precise, sterile, and efficient sampling of cell samples to ensure sample viability and accurate results. Traditional sampling methods are prone to problems such as sample contamination, uneven sample volume, or cumbersome operation. Therefore, a sampling device specifically designed for flow cytometry is needed.
[0003] Chinese patent CN223050922U, published on July 1, 2025, discloses a sampling device for flow cytometry detection. By rotating a worm gear, which drives a worm wheel, one side of the placement plate can be rotated to the upper side of the vertical rod. The mounting block rotates and fits onto the upper side of the wedge-shaped block, preventing the mounting block from being pulled out of the mounting shell. This allows for stable sampling of fluid cells from test tubes placed on the placement plate. Simultaneously, test tubes on the other side of the placement plate can be replaced. The entire process can be performed synchronously, greatly improving work efficiency. However, this... The sampling device used for flow cytometry relies solely on the engagement of wedge blocks and wedge grooves to position the mounting block, lacking an additional locking structure. During rotation, vibration may cause the wedge blocks to detach from the wedge grooves, resulting in misalignment of the mounting block and affecting the accuracy of the sampling position. Furthermore, during sampling, the limiting ring is only fixedly connected to the placement plate via a connecting rod, and its spacing is fixed, making it impossible to adjust its size according to different sampling container specifications. This results in poor container adaptability. If the container diameter does not match the limiting ring, the container may wobble or fail to fit, affecting sampling stability. Utility Model Content
[0004] The purpose of this invention is to provide a sampling device for flow cytometry detection to solve the problems mentioned in the background art.
[0005] To achieve the above objectives, this utility model provides the following technical solution: a sampling device for flow cytometry detection, comprising a base plate, a side plate connected to the top of the base plate, a rotating component disposed on the inner side of the side plate, a horizontal bar fixedly connected to the top of the rotating component, a vertical bar connected to the top of one end of the base plate, a wedge block connected to the top of the vertical bar, mounting shells fixedly connected to both ends of the horizontal bar, mounting blocks engaged inside the mounting shells, a locking assembly disposed between the wedge block and the mounting block, a placement plate fixedly connected to the top of the mounting block, and an adjustable limiting assembly disposed on the surface of the placement plate;
[0006] The adjustable limiting component includes a connecting rod connected above a placement plate. A support rod is installed above the placement plate, and a fixed rod is connected to the inner side of the support rod. A telescopic groove is formed inside the fixed rod, and a damping plate is slidably connected inside the telescopic groove. Damping blocks are connected to the upper and lower sides of the damping plate. A damping groove is formed inside the telescopic groove, and a telescopic rod is connected to the outer surface of the damping plate. A telescopic spring is connected to the outer side of the telescopic rod, and a limiting arc plate is connected to one end of both the telescopic rod and the telescopic spring. A silicone pad is connected to the inner side of the limiting arc plate.
[0007] Preferably, the locking assembly includes a positioning groove, which is formed inside the mounting block. A compression spring is connected to the inner end of the positioning groove, and a limit plate is connected to the other end of the compression spring. A positioning bead is connected to the surface of the limit plate through a connecting rod, and a positioning hole is formed on the upper surface of the wedge block.
[0008] Preferably, one end of the compression spring is welded and fixed to the inner end of the positioning groove, and the other end is welded and fixed to the surface of the limiting plate. When the compression spring is in its natural state, the positioning bead protrudes from the surface of the mounting block.
[0009] Preferably, the outer wall of the limiting plate is fitted with the inner wall of the positioning groove, the limiting plate can slide along the axial direction of the positioning groove, and the diameter of the limiting plate is larger than the diameter of the opening end of the positioning groove.
[0010] Preferably, the connecting rod and the surface of the limiting plate are integrally formed, and the inner diameter of the positioning hole is adapted to the diameter of the positioning bead.
[0011] Preferably, the outer wall of the damping plate is fitted with the inner wall of the expansion groove, and the damping block is slidably connected to the damping groove.
[0012] Preferably, one end of the telescopic rod is welded and fixed to the outer surface of the damping plate, and the other end passes through the fixing rod and is welded to the limiting arc plate. The telescopic spring is sleeved on the outside of the telescopic rod and its two ends abut against the inner wall of the damping plate and the fixing rod, respectively.
[0013] Compared with the prior art, the beneficial effects of this utility model are:
[0014] 1. The sampling device for flow cytometry detection provides additional locking force through the setting of the locking component, preventing the wedge block from disengaging from the wedge groove due to vibration, avoiding the offset of the mounting block, and ensuring the accuracy of the sampling position.
[0015] 2. The sampling device for flow cytometry detection, through the setting of adjustable limiting components, can adjust the limiting distance according to different sampling containers, improve the adaptability to containers, prevent containers from shaking or being unable to be placed, and enhance sampling stability. Attached Figure Description
[0016] Figure 1 This is a side view of the structure of the present utility model;
[0017] Figure 2 This is a schematic diagram of the locking assembly structure of this utility model;
[0018] Figure 3 This is a schematic diagram of the adjustable limiting component structure of this utility model;
[0019] Figure 4 This utility model Figure 3 Enlarged structural diagram at point A in the middle.
[0020] In the diagram: 1. Base plate; 2. Side plate; 3. Rotating component; 4. Horizontal bar; 5. Vertical bar; 6. Wedge block; 7. Mounting shell; 8. Mounting block; 9. Locking assembly; 901. Positioning groove; 902. Compression spring; 903. Limiting plate; 904. Positioning bead; 905. Positioning hole; 10. Placement plate; 11. Adjustable limiting assembly; 1101. Connecting rod; 1102. Support rod; 1103. Fixing rod; 1104. Telescopic groove; 1105. Damping plate; 1106. Damping block; 1107. Damping groove; 1108. Telescopic rod; 1109. Telescopic spring; 1110. Limiting arc plate; 1111. Silicone pad. Detailed Implementation
[0021] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the protection scope of the present utility model.
[0022] Please see Figure 1-4This utility model provides a technical solution: a sampling device for flow cytometry detection, including a base plate 1, a side plate 2 connected to the top of the base plate 1, a rotating component 3 provided on the inner side of the side plate 2, a crossbar 4 fixedly connected to the top of the rotating component 3, a vertical rod 5 connected to the top of one end of the base plate 1, a wedge block 6 connected to the top of the vertical rod 5, mounting shells 7 fixedly connected to both ends of the crossbar 4, mounting blocks 8 engaged inside the mounting shells 7, a locking component 9 provided between the wedge block 6 and the mounting block 8, a placement plate 10 fixedly connected above the mounting block 8, and an adjustable limiting component 11 provided on the surface of the placement plate 10;
[0023] The adjustable limiting assembly 11 includes a connecting rod 1101, which is connected to the top of the placement plate 10. A support rod 1102 is installed above the placement plate 10. A fixing rod 1103 is connected to the inner side of the support rod 1102. A telescopic groove 1104 is formed inside the fixing rod 1103. A damping plate 1105 is slidably connected inside the telescopic groove 1104. Damping blocks 1106 are connected to the upper and lower sides of the damping plate 1105. A damping groove 1107 is formed inside the telescopic groove 1104. The outer surface of the damping plate 1105... A telescopic rod 1108 is connected, and a telescopic spring 1109 is connected to the outer side of the telescopic rod 1108. One end of both the telescopic rod 1108 and the telescopic spring 1109 is connected to a limiting arc plate 1110. A silicone pad 1111 is connected to the inner side of the limiting arc plate 1110. Through the setting of the adjustable limiting component 11, when it is necessary to place sampling containers of different sizes, the container is placed on the placement plate 10 and pushed towards the limiting arc plate 1110. After the limiting arc plate 1110 is pushed, it drives the telescopic rod 1108 to extend and retract into the fixed rod 1103. As the groove 1104 moves, it simultaneously compresses the outer telescopic spring 1109. When the telescopic rod 1108 moves, the damping plate 1105 connected to it slides along the telescopic groove 1104. The damping blocks 1106 on the upper and lower sides of the damping plate 1105 slide synchronously within the telescopic groove 1107. Utilizing the friction between the damping blocks and the damping groove, the damping plate 1105 remains stable during sliding, preventing the limiting arc plate 1110 from wobbling due to sudden changes in the elastic force of the telescopic spring 1109. As the container is placed inside, the elastic force of the telescopic spring 1109 reverses. The limiting arc plate 1110 is used to ensure that it fits tightly against the outer wall of the container. The silicone pad 1111 on the inner side increases the friction with the container through its own elasticity to prevent the container from sliding. When the container is removed, the telescopic spring 1109 returns to its original position, which drives the telescopic rod 1108, the damping plate 1105 and the limiting arc plate 1110 back to their initial positions. This achieves flexible limiting of sampling containers of different diameters and ensures the stability of the container during sampling. The connecting rod 1101 and the support rod 1102 provide stable support for the entire assembly and ensure that the limiting action is performed reliably.
[0024] Furthermore, the locking assembly 9 includes a positioning groove 901, which is located inside the mounting block 8. A compression spring 902 is connected to the inner end of the positioning groove 901, and a limit plate 903 is connected to the other end of the compression spring 902. A positioning bead 904 is connected to the surface of the limit plate 903 via a connecting rod. A positioning hole 905 is provided on the upper surface of the wedge block 6. Through the locking assembly 9, the compression spring 902, made of high-strength alloy spring steel, possesses excellent elastic deformation capability and fatigue resistance, maintaining stable elastic force output during repeated compression and reset. When the mounting block 8 is inserted into the mounting housing 7, the crossbar 4, under the action of the rotating component 3, causes the wedge block 6 to gradually embed into the wedge groove at the lower end of the mounting block 8. At this time, the positioning bead 904 is squeezed by the surface of the wedge block 6, pushing the limit plate 903 towards the inner end of the positioning groove 901 via the connecting rod. The compression spring 902 is compressed and stores elastic potential energy. As the mounting block 8 continues to be inserted, when the positioning bead 904 aligns with the positioning hole 905 on the surface of the wedge block 6, the compression spring 902 releases elastic potential energy, pushing the limiting plate 903, connecting rod, and positioning bead 904 outward, so that the positioning bead 904 is precisely embedded in the positioning hole 905, forming a mechanical lock. This locking method uses the elastic force of the compression spring 902 to ensure that the positioning bead 904 and the positioning hole 905 are tightly engaged, effectively preventing the mounting block 8 from loosening or shifting due to vibration during device operation, and improving the stability of the connection between the mounting block 8 and the mounting shell 7. When it is necessary to remove the mounting block 8, pull the mounting block 8 outward, and the positioning bead 904 is squeezed again and compresses the spring 902, dislodging from the positioning hole 905, realizing convenient disassembly. The material properties of the compression spring 902 ensure that this locking and unlocking process is repeated and reliable.
[0025] Furthermore, one end of the compression spring 902 is welded and fixed to the inner end of the positioning groove 901, and the other end is welded and fixed to the surface of the limiting plate 903. When the compression spring 902 is in its natural state, the positioning bead 904 protrudes from the surface of the mounting block 8. Through the arrangement of the compression spring 902, the limiting plate 903, and the compression spring 902, the compression spring 902 can provide a stable elastic driving force to push the limiting plate 903 to drive the positioning bead 904 to accurately embed into the positioning hole 905, thereby achieving reliable locking. At the same time, the protruding state of the positioning bead 904 in its natural state provides the initial conditions for the locking action, ensuring the smooth triggering of the locking process.
[0026] Furthermore, the outer wall of the limiting plate 903 fits against the inner wall of the positioning groove 901, and the limiting plate 903 can slide along the axial direction of the positioning groove 901. The diameter of the limiting plate 903 is larger than the diameter of the opening end of the positioning groove 901. Through the setting of the positioning groove 901 and the limiting plate 903, the positioning groove 901 provides guidance for the sliding of the limiting plate 903, ensuring its stable axial movement. The design that the diameter of the limiting plate 903 is larger than the diameter of the opening end of the positioning groove 901 can prevent the limiting plate 903 from coming out of the positioning groove 901, ensuring the integrity of the component structure and the reliability of its operation.
[0027] Furthermore, the connecting rod and the surface of the limiting plate 903 are integrally formed, and the inner diameter of the positioning hole 905 is adapted to the diameter of the positioning bead 904. Through the setting of the positioning bead 904 and the positioning hole 905, the integrally formed connecting rod ensures the stability of the connection between the positioning bead 904 and the limiting plate 903, while the adapted diameter allows the positioning bead 904 to be tightly embedded in the positioning hole 905, forming an effective mechanical lock, preventing the mounting block 8 from undergoing relative displacement, and improving the stability of the connection.
[0028] Furthermore, the outer wall of the damping plate 1105 is in contact with the inner wall of the telescopic groove 1104, and the damping block 1106 is slidably connected to the damping groove 1107. Through the setting of the damping block 1106 and the damping groove 1107, the two work together to generate a moderate frictional force, which can buffer the sliding speed of the damping plate 1105 and prevent the limiting arc plate 1110 from shaking rapidly due to the elastic force of the telescopic spring 1109, making the limiting adjustment process more stable and enhancing the stability of the structure.
[0029] Furthermore, one end of the telescopic rod 1108 is welded and fixed to the outer surface of the damping plate 1105, and the other end passes through the fixing rod 1103 and is welded to the limiting arc plate 1110. The telescopic spring 1109 is sleeved on the outside of the telescopic rod 1108 and its two ends abut against the inner walls of the damping plate 1105 and the fixing rod 1103, respectively. Through the setting of the telescopic rod 1108 and the telescopic spring 1109, the telescopic rod 1108 provides guidance for the movement of the limiting arc plate 1110, ensuring its stable movement along a straight line. The telescopic spring 1109 uses elastic deformation to generate a reverse force, pushing the limiting arc plate 1110 to fit tightly against the sampling container, thereby achieving adaptive limiting for containers of different specifications.
[0030] Working principle: During cell testing and sampling, the container to be sampled is first placed on one of the placement plates 10 and fixed by the adjustable limiting component 11. Specifically, the container is pushed towards the limiting arc plate 1110, causing it to move the telescopic rod 1108 into the telescopic groove 1104 within the fixed rod 1103. Simultaneously, the telescopic spring 1109 is compressed. During this process, the damping plate 1105 slides with the telescopic rod 1108 within the telescopic groove 1104. The damping block 1106 and the damping groove 1107 cooperate to generate friction, ensuring the damping... The nylon plate 1105 moves smoothly, preventing the limiting arc plate 1110 from shaking. The reaction force of the telescopic spring 1109 makes the limiting arc plate 1110 fit tightly against the outer wall of the container. The silicone pad 1111 on the inner side increases friction and prevents the container from sliding, thus achieving stable positioning of containers of different sizes. Subsequently, the rotating component 3 drives the crossbar 4 to rotate, causing the mounting block 8 containing the container to move above the wedge block 6. Then, the mounting block 8 is inserted into the mounting shell 7. At this time, the wedge block 6 gradually embeds into the wedge groove at the lower end of the mounting block 8. The positioning bead 904 is pushed by the connecting rod after being squeezed. The limiting plate 903 compresses the compression spring 902. When the positioning bead 904 aligns with the positioning hole 905, the compression spring 902 releases its elastic potential energy, pushing the positioning bead 904 into the positioning hole 905, thus locking and fixing the mounting block 8. This ensures that the mounting block 8 will not loosen or shift during sampling. During sampling, the rotating part 3 can drive the crossbar 4 to rotate, allowing the two mounting blocks 8 to be used alternately. While one is sampling, the other can be used for feeding or changing materials, improving sampling efficiency. After sampling, if it is necessary to change the container, pull the mounting block 8 outwards to release the positioning bead. When spring 902 is compressed and disengaged from positioning hole 905, mounting block 8 can be removed. Repeat the above operation after replacing the container. Throughout the process, components such as base plate 1, side plate 2, and vertical rod 5 provide stable support for the device, ensuring the coordinated operation of each component. Through the cooperation of adjustable limit component 11 and locking component 9, the device can be stably fixed and quickly replaced with containers of different sizes, ensuring that the sampling process is accurate and efficient, meeting the strict requirements of flow cytometry for sample sampling. This completes the usage process of a sampling device for flow cytometry.
[0031] Although embodiments of the present invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made to these embodiments without departing from the principles and spirit of the present invention, the scope of which is defined by the appended claims and their equivalents.
Claims
1. A sampling device for flow cytometry detection, comprising a base plate (1), characterized in that: A side plate (2) is connected above the base plate (1). A rotating part (3) is provided on the inner side of the side plate (2). A crossbar (4) is fixedly connected to the top of the rotating part (3). A vertical rod (5) is connected above one end of the base plate (1). A wedge block (6) is connected to the top of the vertical rod (5). A mounting shell (7) is fixedly connected to both ends of the crossbar (4). A mounting block (8) is engaged inside the mounting shell (7). A locking component (9) is provided between the wedge block (6) and the mounting block (8). A placement plate (10) is fixedly connected above the mounting block (8). An adjustable limiting component (11) is provided on the surface of the placement plate (10). The adjustable limiting component (11) includes a connecting rod (1101) connected above the placement plate (10). A support rod (1102) is installed above the placement plate (10). A fixing rod (1103) is connected to the inner side of the support rod (1102). A telescopic groove (1104) is provided inside the fixing rod (1103). A damping plate (1105) is slidably connected inside the telescopic groove (1104). The upper part of the damping plate (1105) is... Damping blocks (1106) are connected to the lower two sides. A damping groove (1107) is opened on the inner side of the telescopic groove (1104). A telescopic rod (1108) is connected to the outer surface of the damping plate (1105). A telescopic spring (1109) is connected to the outer side of the telescopic rod (1108). One end of the telescopic rod (1108) and the telescopic spring (1109) are connected to a limiting arc plate (1110). A silicone pad (1111) is connected to the inner side of the limiting arc plate (1110).
2. The sampling device for flow cytometry detection according to claim 1, characterized in that: The locking assembly (9) includes a positioning groove (901) which is located inside the mounting block (8). A compression spring (902) is connected to the inner end of the positioning groove (901), and a limit plate (903) is connected to the other end of the compression spring (902). A positioning bead (904) is connected to the surface of the limit plate (903) via a connecting rod. A positioning hole (905) is provided on the upper surface of the wedge block (6).
3. The sampling device for flow cytometry detection according to claim 2, characterized in that: One end of the compression spring (902) is welded and fixed to the inner end of the positioning groove (901), and the other end is welded and fixed to the surface of the limiting plate (903). When the compression spring (902) is in its natural state, the positioning bead (904) protrudes from the surface of the mounting block (8).
4. A sampling device for flow cytometry detection according to claim 2, characterized in that: The outer wall of the limiting plate (903) fits against the inner wall of the positioning groove (901), the limiting plate (903) can slide along the axial direction of the positioning groove (901), and the diameter of the limiting plate (903) is greater than the diameter of the opening end of the positioning groove (901).
5. A sampling device for flow cytometry detection according to claim 2, characterized in that: The connecting rod and the surface of the limiting plate (903) are integrally formed, and the inner diameter of the positioning hole (905) is adapted to the diameter of the positioning bead (904).
6. A sampling device for flow cytometry detection according to claim 1, characterized in that: The outer wall of the damping plate (1105) is fitted with the inner wall of the expansion groove (1104), and the damping block (1106) is slidably connected to the damping groove (1107).
7. A sampling device for flow cytometry detection according to claim 1, characterized in that: One end of the telescopic rod (1108) is welded and fixed to the outer surface of the damping plate (1105), and the other end passes through the fixed rod (1103) and is welded to the limiting arc plate (1110). The telescopic spring (1109) is sleeved on the outside of the telescopic rod (1108) and its two ends abut against the inner walls of the damping plate (1105) and the fixed rod (1103) respectively.