A flow cytometry temperature control device

Abstract

The utility model discloses a kind of flow cytometry detection temperature control devices, it is related to cell detection equipment field, including flow cytometry, the flow cytometry end is equipped with detection chamber, the inside of detection chamber is equipped with the temperature control mechanism for adjusting and controlling detection tube temperature, the temperature control mechanism includes the base of sliding installation in the bottom of detection chamber, and base outer wall is rotatably installed with temperature control box, and temperature control box top is fixedly installed with rack, the heating plate is equipped in the bottom of temperature control box, and temperature control box inner wall is equipped with semiconductor refrigeration piece, the inside of rack is equipped with the clamping mechanism for the limiting of detection tube. This flow cytometry detection temperature control device, accurate temperature control is carried out to detection tube, effectively solve the problem that detection tube temperature is uncontrollable in prior art, significantly improve the stability and reliability of cell function detection data, ensure the accuracy of detection result, provide strong guarantee for high-precision experiment.

Description

Technical Field

[0001] This utility model relates to the field of cell detection equipment technology, specifically a flow cytometry detection temperature control device. Background Technology

[0002] Flow cytometry is a high-speed, one-to-one quantitative analysis and sorting of single cells or other biological particles in a suspension by detecting the fluorescent signals of labeled cells. It enables rapid detection of the physical and chemical properties of cells and is widely used in fields such as biology, medicine, and immunology. It usually requires the use of a flow cytometer.

[0003] In the prior art, Chinese Patent No. CN213388673U discloses a stem cell flow cytometer, including an instrument body, a detection solution on one side of the instrument body, a detection head inside a corner of the instrument body, a protective device on the instrument body located at the detection head, a laser probe inside the instrument body located opposite the detection head, and a lifting device on the instrument body located on one side of the detection head. Multiple experimental samples can be placed in a fixed hole using the lifting device, and detection can be performed using a telescopic rod. Samples can be changed by rotating the fixed plate, which is convenient and saves experimental time. The protective device, by pulling the movable cover, allows it to cooperate with the fixed cover, protecting the detection head and preventing damage, thus ensuring safety.

[0004] Based on the above information, existing flow cytometers typically expose the detection tubes directly to the external environment at room temperature during detection, making effective temperature control impossible. Flow cytometry is extremely sensitive to temperature conditions; when the temperature of the flow cytometer sample tubes is uncontrollable, it directly causes significant fluctuations and instability in cell function detection data. This not only interferes with the reliability of the detection results but may also lead to erroneous conclusions, severely impacting the accuracy and scientific validity of flow cytometry detection. Therefore, we propose a flow cytometry detection temperature control device. Utility Model Content

[0005] The purpose of this invention is to provide a flow cytometry detection temperature control device to solve the problem mentioned in the background art that, during the detection of existing flow cytometry instruments, the detection tube is usually directly exposed to the external environment and is completely at room temperature, which makes it impossible to effectively control the temperature. Flow cytometry analysis is extremely sensitive to temperature conditions. When the temperature of the flow cytometry sample tube is uncontrollable, it will directly cause significant fluctuations and instability in the cell function detection data. This will not only interfere with the reliability of the detection results, but may even lead to erroneous conclusions, seriously affecting the accuracy and scientific nature of flow cytometry detection.

[0006] To achieve the above objectives, this utility model provides the following technical solution: a flow cytometry detection and temperature control device, comprising a flow cytometer, wherein the flow cytometer is provided with a detection chamber at its end, and the detection chamber is provided with a temperature control mechanism for regulating the temperature of the detection tube, the temperature control mechanism comprising a base slidably installed at the bottom of the detection chamber, a temperature control box rotatably installed on the outer wall of the base, and a placement rack fixedly installed on the top of the temperature control box, a heating plate provided at the bottom of the temperature control box, and a semiconductor cooling chip provided on the inner wall of the temperature control box, and a clamping mechanism for limiting the position of the detection tube provided inside the placement rack.

[0007] Furthermore, the temperature control box is cylindrical in shape and has a temperature control cavity inside. The placement rack has a circular cross-section, and its top is flush with the top of the temperature control box. The diameter of the placement rack is the same as the inner diameter of the temperature control box.

[0008] Furthermore, the outer wall of the placement rack is provided with placement holes at equal angles, and the diameter of the placement holes is larger than the diameter of the detection tube. The top corner of the placement holes is designed at an angle. The bottom of the temperature control box is provided with a placement groove, and the inner wall of the placement groove is in contact with the bottom outer wall of the detection tube. The placement groove is set at equal angles.

[0009] Furthermore, the thermoelectric cooler covers the inner wall of the temperature control chamber, with the cooling surface of the thermoelectric cooler facing the detection tube, and both the thermoelectric cooler and the heating plate are electrically connected to the control panel.

[0010] Furthermore, a sliding rod is fixedly installed at the bottom of the base, and a limiting block is provided at the end of the sliding rod. The sliding rod has a T-shaped cross-section, and two sets of sliding rods are symmetrically arranged on the outer wall of the bottom of the base. The limiting block is perpendicular to the sliding rod. A sliding groove and a limiting groove are opened at the bottom of the detection chamber. The inner wall of the sliding groove is in contact with the outer wall of the sliding rod, and the end of the sliding groove is open. The inner wall of the limiting groove is in contact with the outer wall of the limiting block, and the length of the limiting groove is less than the length of the sliding groove. The limiting groove and the sliding groove are connected.

[0011] Furthermore, the clamping mechanism includes a rotating shaft slidably installed inside the placement frame, and a clamping roller is rotatably installed on the outer wall of the rotating shaft. A guide rod is fixedly installed inside the placement frame, and a compression spring is sleeved on the outer wall of the guide rod.

[0012] Furthermore, the clamping rollers are arranged at equal angles on the inner wall of the placement hole, and the outer wall of the clamping rollers is in contact with the outer wall of the detection tube. The clamping rollers are made of rubber, and the rotating shaft is slidably connected to the guide rod.

[0013] Furthermore, the guide rods are symmetrically arranged at both ends of the rotating shaft, and the guide rods are perpendicular to the rotating shaft. One end of the compression spring is in contact with the outer wall of the rotating shaft, and the other end of the compression spring is in contact with the inside of the placement frame.

[0014] Compared with the prior art, the beneficial effects of this utility model are:

[0015] 1. This flow cytometry detection temperature control device, by setting up a temperature control mechanism, uses a heating plate and a semiconductor cooling chip inside the temperature control chamber to precisely control the temperature of the detection tube, effectively solving the problem of uncontrollable detection tube temperature in existing technologies, significantly improving the stability and reliability of cell function detection data, ensuring the accuracy of detection results, and providing strong support for high-precision experiments.

[0016] 2. By setting a sliding base and a rotating temperature control box, the temperature control mechanism can move and rotate flexibly, which makes it easy for operators to place and take out the test tube. At the same time, it can also make the test tube evenly heated or cooled in the temperature control box, further improving the temperature control effect and optimizing the testing process.

[0017] 3. The clamping mechanism utilizes equally angled rubber clamping rollers and compression springs to adaptively clamp test tubes of different diameters, preventing the test tubes from shaking or shifting during the test and ensuring the stability of the test process. At the same time, the rubber clamping rollers can also avoid damaging the test tubes and protect the integrity of the samples. Attached Figure Description

[0018] Figure 1 This is a schematic diagram of the overall structure of this utility model;

[0019] Figure 2 This is a schematic diagram of the slide groove and limiting groove structure of this utility model;

[0020] Figure 3 This is a schematic diagram of the temperature control box, placement rack, base, slide bar, and limiting block of this utility model;

[0021] Figure 4 This is a cross-sectional structural diagram of the temperature control box and the placement rack of this utility model;

[0022] Figure 5 This is a schematic diagram of the structure of the placement frame, placement hole, and clamping roller of this utility model;

[0023] Figure 6 This is a schematic diagram of the cross-sectional structure of the placement rack of this utility model;

[0024] Figure 7 This utility model Figure 1 Enlarged structural diagram at point A in the middle.

[0025] In the diagram: 1. Flow cytometer; 101. Detection chamber; 102. Control panel; 2. Temperature control chamber; 201. Placement slot; 3. Base; 301. Slide bar; 302. Limiting block; 4. Slide groove; 401. Limiting groove; 5. Semiconductor cooling chip; 6. Heating plate; 7. Placement rack; 701. Placement hole; 8. Clamping roller; 801. Rotating shaft; 9. Guide rod; 901. Compression spring. Detailed Implementation

[0026] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the protection scope of the present utility model.

[0027] Example 1: Please refer to Figures 1-4 The present invention provides the following technical solution: a flow cytometer detection temperature control device, including a flow cytometer 1, a detection chamber 101 at the end of the flow cytometer 1, a temperature control mechanism for regulating the temperature of the detection tube inside the detection chamber 101, the temperature control mechanism including a base 3 slidably installed at the bottom of the detection chamber 101, a temperature control box 2 rotatably installed on the outer wall of the base 3, a placement rack 7 fixedly installed on the top of the temperature control box 2, a heating plate 6 at the bottom of the temperature control box 2, and a semiconductor cooling chip 5 on the inner wall of the temperature control box 2.

[0028] like Figures 1-4 As shown, the temperature control box 2 has a cylindrical design and a temperature control cavity inside. The placement rack 7 has a circular cross-section, and its top is flush with the top of the temperature control box 2. The diameter of the placement rack 7 is the same as the inner diameter of the temperature control box 2. Placement holes 701 are opened at equal angles on the outer wall of the placement rack 7. The diameter of the placement holes 701 is larger than the diameter of the detection tube, and the top corners of the placement holes 701 are beveled. A placement groove 201 is opened at the bottom of the temperature control box 2. The inner wall of the placement groove 201 fits against the outer wall of the bottom of the detection tube, and the placement groove 201 is set at equal angles. The thermoelectric cooler 5 covers the inner wall of the temperature control box 2, and the cooling surface of the thermoelectric cooler 5 faces the detection tube. The thermoelectric cooler 5 and the heating plate 6 are both electrically connected to the control panel 102.

[0029] like Figures 1-3As shown, a sliding rod 301 is fixedly installed at the bottom of the base 3, and a limiting block 302 is provided at the end of the sliding rod 301. The sliding rod 301 has a T-shaped cross-section, and two sets of sliding rods 301 are symmetrically arranged on the outer wall of the bottom of the base 3. The limiting block 302 is perpendicular to the sliding rod 301. A sliding groove 4 and a limiting groove 401 are opened at the bottom of the detection chamber 101. The inner wall of the sliding groove 4 is in contact with the outer wall of the sliding rod 301, and the end of the sliding groove 4 is open. The inner wall of the limiting groove 401 is in contact with the outer wall of the limiting block 302, and the length of the limiting groove 401 is less than the length of the sliding groove 4, and the limiting groove 401 is connected to the sliding groove 4.

[0030] When the temperature control mechanism is working, the operator can preset the target temperature required for detection through the control panel 102. After the detection tube is placed in the placement slot 201 in the temperature control box 2 and fixed by the clamping mechanism, the heating plate 6 at the bottom of the temperature control box 2 and the semiconductor cooling chip 5 on the inner wall will work together under the control of the control panel 102. If the temperature in the temperature control chamber is lower than the target temperature, the heating plate 6 will start and release heat to gradually increase the temperature. If the temperature is higher than the target temperature, the cooling surface of the semiconductor cooling chip 5 will release cold energy towards the detection tube to reduce the temperature in the temperature control chamber. The base 3 can slide along the sliding groove 4 in the detection chamber 101 through the bottom slide rod 301, which can push the temperature control box 2 to the detection position or pull it out to the operation position to meet the position adjustment needs during the detection process and achieve accurate and stable control of the temperature of the detection tube.

[0031] Example 2: Please refer to Figure 1 and Figures 4-7 Based on Embodiment 1, a clamping mechanism is also disclosed, the specific structure of which is as follows: The placement frame 7 is provided with a clamping mechanism for limiting the detection tube. The clamping mechanism includes a rotating shaft 801 slidably installed inside the placement frame 7, and a clamping roller 8 is rotatably installed on the outer wall of the rotating shaft 801. A guide rod 9 is fixedly installed inside the placement frame 7, and a compression spring 901 is sleeved on the outer wall of the guide rod 9. The clamping roller 8 is set at equal angles on the inner wall of the placement hole 701, and the outer wall of the clamping roller 8 is in contact with the outer wall of the detection tube. The clamping roller 8 is made of rubber. The rotating shaft 801 is slidably connected to the guide rod 9. The guide rod 9 is symmetrically arranged at both ends of the rotating shaft 801, and the guide rod 9 is perpendicular to the rotating shaft 801. One end of the compression spring 901 is in contact with the outer wall of the rotating shaft 801, and the other end of the compression spring 901 is in contact with the inside of the placement frame 7.

[0032] The clamping mechanism automatically starts during the placement of the test tube. When the test tube is inserted into the placement hole 701 at the top of the placement rack 7, its outer wall will press against the clamping rollers 8 that are evenly distributed on the inner wall of the placement hole 701. After being pressed, the clamping rollers 8 slide into the placement rack 7 along the guide rod 9 via the rotating shaft 801. At this time, the compression spring 901 on the outer wall of the guide rod 9 is compressed and generates a reverse elastic force. Under the action of the elastic force, the rotating shaft 801 drives the clamping rollers 8 to fit tightly against the outer wall of the test tube. The friction of the rubber material forms a stable clamp on the test tube. Since the elastic force of the compression spring 901 can be adaptively adjusted according to the change of the test tube diameter, it can be adapted to test tubes of different specifications. This not only prevents the test tube from shaking and shifting during the rotation or movement of the temperature control box 2, but also prevents the test tube from being damaged by excessive clamping force, ensuring the stability and safety of the test tube and the sample inside.

[0033] In the description of this utility model, it should be noted that, unless otherwise explicitly specified and limited, the terms "connected" and "linked" should be interpreted broadly. For example, they can refer to a fixed connection, a detachable connection, or an integral connection; they can refer to a mechanical connection or an electrical connection; they can refer to a direct connection or an indirect connection through an intermediate medium. Those skilled in the art can understand the specific meaning of the above terms in this utility model based on the specific circumstances.

[0034] Although the present invention has been described in detail with reference to the foregoing embodiments, those skilled in the art can still modify the technical solutions described in the foregoing embodiments or make equivalent substitutions for some of the technical features. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of the present invention should be included within the protection scope of the present invention.

Claims

1. A flow cytometry detection and temperature control device, comprising a flow cytometer (1), wherein the flow cytometer (1) is provided with a detection chamber (101) at its end, characterized in that: The detection chamber (101) is equipped with a temperature control mechanism for regulating the temperature of the detection tube. The temperature control mechanism includes a base (3) that is slidably installed at the bottom of the detection chamber (101), and a temperature control box (2) is rotatably installed on the outer wall of the base (3). A placement rack (7) is fixedly installed on the top of the temperature control box (2). A heating plate (6) is provided at the bottom of the temperature control box (2), and a semiconductor cooling chip (5) is provided on the inner wall of the temperature control box (2). A clamping mechanism for limiting the detection tube is provided inside the placement rack (7).

2. The flow cytometry detection and temperature control device according to claim 1, characterized in that: The temperature control box (2) is cylindrical in shape and has a temperature control cavity inside. The placement rack (7) has a circular cross-section and the top of the placement rack (7) is flush with the top of the temperature control box (2). The diameter of the placement rack (7) is the same as the inner diameter of the temperature control box (2).

3. The flow cytometry detection and temperature control device according to claim 1, characterized in that: The outer wall of the placement rack (7) is provided with placement holes (701) at equal angles, and the diameter of the placement holes (701) is larger than the diameter of the detection tube. The top corner of the placement holes (701) is designed with an angle. The bottom of the temperature control box (2) is provided with a placement groove (201), and the inner wall of the placement groove (201) is in contact with the bottom outer wall of the detection tube. The placement groove (201) is set at equal angles.

4. The flow cytometry detection and temperature control device according to claim 1, characterized in that: The semiconductor cooling chip (5) covers the inner wall of the temperature control box (2), and the cooling surface of the semiconductor cooling chip (5) faces the detection tube. The semiconductor cooling chip (5) and the heating plate (6) are both electrically connected to the control panel (102).

5. A flow cytometry detection and temperature control device according to claim 1, characterized in that: The base (3) is fixedly installed with a slide rod (301) at the bottom, and a limiting block (302) is provided at the end of the slide rod (301). The slide rod (301) has a T-shaped cross section, and two sets of slide rods (301) are symmetrically arranged on the outer wall of the bottom of the base (3). The limiting block (302) is perpendicular to the slide rod (301). The bottom of the detection chamber (101) is provided with a slide groove (4) and a limiting groove (401). The inner wall of the slide groove (4) is in contact with the outer wall of the slide rod (301), and the end of the slide groove (4) is open. The inner wall of the limiting groove (401) is in contact with the outer wall of the limiting block (302), and the length of the limiting groove (401) is less than the length of the slide groove (4). The limiting groove (401) is connected to the slide groove (4).

6. A flow cytometry detection and temperature control device according to claim 1, characterized in that: The clamping mechanism includes a rotating shaft (801) that is slidably installed inside the placement frame (7), and a clamping roller (8) is rotatably installed on the outer wall of the rotating shaft (801). A guide rod (9) is fixedly installed inside the placement frame (7), and a compression spring (901) is sleeved on the outer wall of the guide rod (9).

7. A flow cytometry detection and temperature control device according to claim 6, characterized in that: The clamping roller (8) is set at equal angles on the inner wall of the placement hole (701), and the outer wall of the clamping roller (8) is in contact with the outer wall of the detection tube. The clamping roller (8) is made of rubber, and the rotating shaft (801) is slidably connected to the guide rod (9).

8. A flow cytometry detection and temperature control device according to claim 7, characterized in that: The guide rod (9) is symmetrically arranged at both ends of the rotating shaft (801), and the guide rod (9) is perpendicular to the rotating shaft (801). One end of the compression spring (901) is in contact with the outer wall of the rotating shaft (801), and the other end of the compression spring (901) is in contact with the inside of the placement rack (7).

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