A light-excited chemiluminescence signal detection device

Abstract

The utility model discloses a kind of photoexcitation chemiluminescence signal detection devices, including main casing, light-shield front cover assembly and light-shield rear cover are respectively installed in both ends of main casing, excitation light source transmitter and temperature control module are installed on the mounting plate of main casing, the front end of excitation light source transmitter is installed with light path detection module, detection chip is installed on temperature control module, working hole is opened on the front end face of light path detection module, temperature control module includes installation base, a pair of support plate is installed on the upper installation of installation base, receiving end plate is installed in one end of a pair of support plate, light hole is opened on receiving end plate, light hole is coaxially arranged with working hole, heating gasket is uniformly set on the end face of a pair of support plate opposite, detection chip includes sample cell and support body, support body is set between a pair of support plate, sample cell is set between a pair of heating gasket, this detection device compact structure, it is convenient to carry, reduce signal interference and background noise, improve the sensitivity of detection, reduce the influence of temperature fluctuation on detection result.

Description

Technical Field

[0001] This utility model belongs to the field of detection technology and relates to a detection device, specifically a photoexcited chemiluminescence signal detection device. Background Technology

[0002] Photochemically initiated luminescence (PRC) is a process that uses light of a specific wavelength to trigger a chemical reaction, generating an excited-state intermediate, which in turn causes the luminescent material to release visible light. It is widely used in environmental monitoring, biomedicine, and materials science. However, the complex structure of existing detection devices results in large equipment size, high price, and high maintenance costs. They are not portable or instantaneous for field work. The experimental details are complex to control and require professional personnel to operate. During the detection process, the contact between the temperature control module and the detection chip is unstable, resulting in poor temperature control, easy interference with experimental results, and low reproducibility. Summary of the Invention

[0003] In view of the shortcomings of the existing technology, the purpose of this utility model is to provide a photoexcited chemiluminescence signal detection device, which can solve the technical problems of existing devices having complex structure, poor temperature control, lack of portability and high production and maintenance costs.

[0004] To solve the above-mentioned technical problems, the present invention adopts the following technical solution:

[0005] A photoexcited chemiluminescence signal detection device includes a main housing open at both ends, a light-shielding front cover assembly installed at the front end of the main housing, a light-shielding rear cover installed at the rear end of the main housing, a mounting plate disposed inside the main housing, an excitation light source emitter and a temperature control module mounted on the mounting plate, an optical path detection module mounted at the front end of the excitation light source emitter, and a detection chip mounted on the temperature control module.

[0006] The excitation source emitter is used to provide a stable excitation source;

[0007] The optical path detection module is used for directional transmission of excitation light and directional reception of chemiluminescence signals, and for detecting the chemiluminescence signals;

[0008] The upper surface of the mounting plate is provided with a first mounting platform and a second mounting platform. The excitation light source emitter is mounted on the first mounting platform through a bracket. The front end of the optical path detection module is located above the second mounting platform. A working hole is opened on the front end face of the optical path detection module. The working hole is both a light source emission hole and a signal receiving hole.

[0009] The temperature control module includes a mounting base, on the upper surface of which a pair of symmetrically arranged L-shaped support plates are mounted. The two support plates are connected by a receiving end plate at the ends facing the optical path detection module. The receiving end plate has a light-transmitting hole, which is coaxial with the working hole. Heating pads are provided on the opposite end faces of the two support plates.

[0010] The detection chip includes a transparent sample cell and a support, with the support disposed between a pair of support plates and the sample cell disposed between a pair of heating pads.

[0011] This utility model also includes the following technical features:

[0012] The upper surface of the second mounting platform is provided with a U-shaped mounting groove, the opening end of which faces the front end of the second mounting platform. The lower surface of the mounting base is provided with a positioning hole, and the upper surface of the mounting groove is provided with a positioning protrusion corresponding to the positioning hole.

[0013] The detection chip also includes a light guide assembly, which is disposed between the sample cell and the support.

[0014] The detection chip also includes a light guide plate at the same height as the sample cell. Multiple horizontally arranged light guide grooves are provided on the left and right side walls of the light guide plate. One end of the light guide plate is fixedly connected to the side wall of the sample cell, and the other end of the light guide plate is fixedly connected to the side wall of the support.

[0015] The sample cell is a single-cell structure with a volume of 20-500 μl.

[0016] A U-shaped receiving opening is provided on the mounting base between the pair of support plates. A plug-in groove is provided at the bottom of the receiving opening. The support body includes a hand-held plate. The bottom of the hand-held plate is integrally connected to the base. A plug-in protrusion is provided at the bottom of the base. The plug-in protrusion cooperates with the plug-in groove to install the support body into the receiving opening.

[0017] The second mounting platform has a support platform at its front end. The upper surface of the support platform is flush with the upper surface of the mounting groove, and the lower surface of the base is in contact with the upper surface of the support platform.

[0018] The aforementioned light-shielding front cover assembly includes a fixed frame and a light-shielding front cover that are hinged together. The fixed frame is installed at the front end of the main housing. A flexible U-shaped clamping plate is installed on the top of the fixed frame. A pair of arc-shaped protrusions are provided on the inner side of the front end of the U-shaped clamping plate. A locking plate is installed on the top of the light-shielding front cover. The end of the locking plate is an arc-shaped snap connector. The arc-shaped snap connector cooperates with the U-shaped clamping plate to fix the light-shielding front cover to the fixed frame. A handle is installed on the front end face of the light-shielding front cover.

[0019] The top of the receiving end plate is higher than the top of the pair of support plates, and a temperature sensor is installed on the side wall of the receiving end plate facing the support plates.

[0020] A wiring box is installed on the rear end face of the light-shielding back cover. An electronic wire bundle is installed inside the wiring box. One end of the electronic wire bundle passes through the light-shielding back cover and is electrically connected to the excitation light source emitter, the optical path detection module, and the temperature control module.

[0021] Both ends of the fixed frame are provided with grooves along the circumference, and light-dense pads are installed in the grooves. The ends of the main shell and the light-shielding front cover are respectively inserted into the grooves and abut against the light-dense pads.

[0022] Compared with the prior art, this utility model has the following technical effects:

[0023] (I) The overall structure is compact, and all components are assembled and installed on the mounting plate to achieve an integrated design, which greatly reduces the size of the device, makes it easy to carry when working in the field, and is easy to operate and has low maintenance costs.

[0024] (II) The structure of the temperature control module improves the accuracy and stability of installation. Except for the light-transmitting hole, the detection chip is effectively shielded to avoid signal interference, reduce background noise, improve detection sensitivity, provide a constant temperature environment for the sample cell, and reduce the impact of temperature fluctuations on the detection results.

[0025] (III) The structural design of the detection chip can increase the heat-receiving area and photosensitive area of ​​the sample cell, improve the sensitivity of signal acquisition, and the support body ensures the stability of installation while facilitating sample addition and hand operation. No auxiliary tools are required, making operation simpler.

[0026] (IV) The light-dense pad inside the light-shielding front cover assembly improves the light-shielding performance of the light-shielding front cover assembly, avoids interference from external light, and makes the light-shielding front cover assembly form a light-proof reaction chamber, increasing the excitation efficiency. It is also securely installed and easy to open and close. Attached Figure Description

[0027] Figure 1 This is an overall explosion diagram of the present invention. Figure 1 .

[0028] Figure 2 This is an overall explosion diagram of the present invention. Figure 2 .

[0029] Figure 3 This is a schematic diagram of the overall structure of this utility model.

[0030] Figure 4 This is a cross-sectional view of the present invention.

[0031] Figure 5This is a schematic diagram of the temperature control module structure of this utility model.

[0032] Figure 6 This is a schematic diagram of the working principle of the optical path detection module of this utility model.

[0033] The meanings of the labels in the diagram are as follows:

[0034] 1. Main housing; 2. Front light shield assembly; 3. Rear light shield cover; 4. Mounting plate; 5. Excitation light source emitter; 6. Temperature control module; 7. Optical path detection module; 8. Detection chip; 9. Wiring box; 10. Electronic wire harness; 11. Light-dense pad.

[0035] 21. Fixed frame; 22. Front shading cover; 23. U-shaped clamp; 24. Arc-shaped protrusion; 25. Locking plate; 26. Arc-shaped snap connector; 27. Handle.

[0036] 41. First mounting platform; 42. Second mounting platform; 43. Mounting groove; 44. Positioning protrusion; 45. Support platform;

[0037] 61. Mounting base; 62. Support plate; 63. Receiver end plate; 64. Light transmission hole; 65. Heating pad; 66. Receiving opening; 67. Insertion slot; 68. Temperature sensor.

[0038] 71. Working hole;

[0039] 81. Sample cell; 82. Support body; 83. Light guide plate; 84. Light guide groove; 821. Handheld plate; 822. Base; 823. Insertion protrusion.

[0040] The specific content of this utility model will be further explained in detail below with reference to the embodiments. Detailed Implementation

[0041] Following the above technical solution, the following are specific embodiments of this utility model. It should be noted that this utility model is not limited to the following specific embodiments, and all equivalent modifications made based on the technical solution of this application fall within the protection scope of this utility model.

[0042] In this utility model, unless otherwise stated, directional terms such as "upper", "lower", "left", "right", "front", and "rear" are generally defined based on the drawing in the corresponding figure, and "inner" and "outer" refer to the inner and outer contours of the corresponding components.

[0043] Example:

[0044] This embodiment provides a photoexcited chemiluminescence signal detection device, such as... Figures 1 to 6As shown, it includes a main housing 1 open at both ends, a light-shielding front cover assembly 2 installed at the front end of the main housing 1, a light-shielding rear cover 3 installed at the rear end of the main housing 1, an installation plate 4 provided inside the main housing 1, an excitation light source emitter 5 and a temperature control module 6 installed on the installation plate 4, an optical path detection module 7 installed at the front end of the excitation light source emitter 5, and a detection chip 8 installed on the temperature control module 6.

[0045] The upper surface of the mounting plate 4 is provided with a first mounting platform 41 and a second mounting platform 42. The excitation light source emitter 5 is mounted on the first mounting platform 41 by a bracket. The front end of the optical path detection module 7 is located above the second mounting platform 42. A working hole 71 is opened on the front end surface of the optical path detection module 7. The working hole 71 is both a light source emission hole and a signal receiving hole.

[0046] The temperature control module 6 includes a mounting base 61. A pair of symmetrically arranged L-shaped support plates 62 are mounted on the upper surface of the mounting base 61. The two support plates 62 are connected by a receiving end plate 63 at the ends facing the optical path detection module 7. A light-transmitting hole 64 is opened on the receiving end plate 63. The light-transmitting hole 64 is coaxially arranged with the working hole 71. A heating pad 65 is provided on the opposite end faces of the two support plates 62.

[0047] The detection chip 8 includes a transparent sample cell 81 and a support 82, with the support 82 disposed between a pair of support plates 62 and the sample cell 81 disposed between a pair of heating pads 65.

[0048] In this embodiment, as shown Figure 6 As shown, the excitation source emitter 5 is used to provide a stable excitation source; the optical path detection module 7 is used to directionally transmit the excitation light emitted by the excitation source emitter 5 to the sample cell 81 through a collimating lens, and then directionally receive the chemiluminescence signal generated after the sample is excited, and finally detect the chemiluminescence signal. The detector in the optical path detection module 7 adopts a high-sensitivity photomultiplier tube (PMT) or silicon photomultiplier tube (MPPC), which can improve the sensitivity of the detection device and support the detection of trace substances. The main housing 1, combined with the light-shielding front cover assembly 2 and the light-shielding rear cover 3, provides a light-proof environment for the detection process, reduces environmental interference, and improves experimental reproducibility. By making reasonable use of the internal space of the housing, the excitation light source emitter 5, temperature control module 6, optical path detection module 7, and detection chip 8 are integrated and mounted on the mounting plate 4, achieving an integrated design that greatly reduces the size of the device and makes it easy to carry in the field. The coaxial arrangement of the light-transmitting hole 64 and the working hole 71 ensures the directional transmission and reception of excitation light and chemiluminescence signals. During the detection process, the divergent light in the sample cell 81 is blocked by the receiving end plate 63 and a pair of support plates 62 to avoid signal interference, reduce background noise, and improve detection sensitivity.

[0049] Furthermore, the top of the receiving end plate 63 is higher than the top of the pair of support plates 62. A temperature sensor 68 is installed on the side wall of the receiving end plate 63 facing the support plate 62, which can ensure that the pair of heating pads 65 provide a constant temperature environment for the sample cell 81 and reduce the impact of temperature fluctuations on the detection results.

[0050] Furthermore, a U-shaped mounting groove 43 is provided on the upper surface of the second mounting platform 42, with the opening end of the mounting groove 43 facing the front end of the second mounting platform 42. A positioning hole is provided on the lower surface of the mounting base 61, and a positioning protrusion 44 corresponding to the positioning hole is provided on the upper surface of the mounting groove 43, which improves the stability of the temperature control module 6 and ensures the accuracy of installation.

[0051] As a preferred embodiment, the detection chip 8 further includes a light guide plate 83 at the same height as the sample cell 81. Multiple horizontally arranged light guide grooves 84 are formed on the left and right sidewalls of the light guide plate 83. One end of the light guide plate 83 is fixedly connected to the sidewall of the sample cell 81, and the other end is fixedly connected to the sidewall of the support 82. After the excitation light emitted by the excitation source emitter 5 passes through the sample cell 81, the light guide grooves 84 can guide the unabsorbed or unscattered excitation light out along the light guide grooves 84, avoiding affecting the optical path efficiency. The chemiluminescence signal generated after the sample is excited is in a scattered state. Some of the chemiluminescence signal is reflected when it hits the sidewall of the light guide groove 84, which can directionally transmit the chemiluminescence signal to the optical path detection module 7 for capture, reducing background noise, increasing signal strength, reducing the risk of false negatives, and improving detection accuracy.

[0052] Furthermore, the left and right sidewalls of the sample cell 81 are outwardly convex arc-shaped structures, which are in contact with a pair of heating pads 65. The arc-shaped structure can increase the heating area of ​​the sample cell 81 by 30%, reducing heat consumption. At the same time, the photosensitive area can be expanded by 25%, increasing the area of ​​the sample in the sample cell 1 that is excited, thereby improving the sensitivity of signal acquisition. The sample cell 81 is a single-cell structure with a volume of 20-500 μl, improving portability. The sample dispensing port of the sample cell 81 can be sealed with a flexible cap to prevent sample leakage.

[0053] As a preferred embodiment, the mounting base 61 between the pair of support plates 62 is provided with a U-shaped receiving opening 66, and the bottom of the receiving opening 66 is provided with an insertion groove 67. The support body 82 includes a hand-held plate 821, which facilitates sample addition and hand operation without the need for auxiliary tools. The bottom of the hand-held plate 821 is integrally connected to the base 822, and the bottom of the base 822 is provided with an insertion protrusion 823. The insertion protrusion 823 cooperates with the insertion groove 67 to stably install the support body 82 in the receiving opening 66.

[0054] The front end of the second mounting platform 42 is provided with a support platform 45. The upper surface of the support platform 45 is on the same plane as the upper surface of the mounting groove 43. The lower surface of the base 822 is in contact with the upper surface of the support platform 45, thereby improving the installation stability of the detection chip 8.

[0055] As a preferred embodiment, the light-shielding front cover assembly 2 in this embodiment includes a fixed frame 21 and a light-shielding front cover 22 that are hinged to each other. The fixed frame 21 is installed at the front end of the main housing 1. An elastic U-shaped card plate 23 is installed on the top of the fixed frame 21. A pair of arc-shaped protrusions 24 are provided on the inner side of the front end of the U-shaped card plate 23. A locking plate 25 is installed on the top of the light-shielding front cover 22. The end of the locking plate 25 is an arc-shaped snap connector 26. The arc-shaped snap connector 26 cooperates with the U-shaped card plate 23 to fix the light-shielding front cover 22 to the fixed frame 21. A handle 27 is installed on the front end face of the light-shielding front cover 22 for easy opening and closing.

[0056] Furthermore, both ends of the fixed frame 21 are provided with grooves along the circumference, and light-dense pads 11 are installed in the grooves. The ends of the main housing 1 and the light-shielding front cover 22 are respectively inserted into the grooves and abut against the light-dense pads 11. The setting of the light-dense pads 11 improves the light-shielding performance of the light-shielding front cover assembly 2, avoids external light interference, and makes the light-shielding front cover assembly 2 form a light-proof reaction chamber, thereby increasing the excitation efficiency.

[0057] As a preferred embodiment, a wiring box 9 is installed on the rear end face of the light-shielding back cover 3. An electronic wire bundle 10 is provided inside the wiring box 9. One end of the electronic wire bundle 10 passes through the light-shielding back cover 3 and is electrically connected to the excitation light source emitter 5, the optical path detection module 7 and the temperature control module 6.

[0058] In actual operation of this embodiment:

[0059] 1. Install the temperature control module 6 into the mounting slot 43, ensuring that the light transmission hole 64 and the working hole 71 are coaxially aligned;

[0060] 2. Hold the handheld plate 821 with one hand, add the sample into the sample cell 81, seal the sample cell 81 with a flexible cap, install the detection chip 8 into the temperature control module 6, and the side wall of the sample cell 81 is in contact with the heating pad 65.

[0061] 3. Close the light-shielding front cover 22 and connect the electronic wiring harness 10 to the external power supply;

[0062] 4. Start the excitation light source emitter 5 to generate excitation light of a specific wavelength. After being collimated by the optical path detection module 7, the excitation light is emitted from the working hole 71 and passes through the light transmission hole 64 to irradiate the sample in the sample cell 81. After the sample is excited, it generates a chemiluminescence signal. The chemiluminescence signal is then captured and detected by the optical path detection module 7 through the light transmission hole 64 and the working hole 71.

Claims

1. A photoexcited chemiluminescence signal detection device, comprising a main housing (1) open at both ends, a light-shielding front cover assembly (2) installed at the front end of the main housing (1), and a light-shielding rear cover (3) installed at the rear end of the main housing (1), characterized in that, The main housing (1) is provided with an installation plate (4), on which an excitation light source emitter (5) and a temperature control module (6) are installed. An optical path detection module (7) is installed at the front end of the excitation light source emitter (5), and a detection chip (8) is installed on the temperature control module (6). The excitation source emitter (5) is used to provide a stable excitation source; The optical path detection module (7) is used for directional transmission of excitation light and directional reception of chemiluminescence signals, and for detecting the chemiluminescence signals; The upper surface of the mounting plate (4) is provided with a first mounting platform (41) and a second mounting platform (42). The excitation light source emitter (5) is mounted on the first mounting platform (41) by a bracket. The front end of the optical path detection module (7) is located above the second mounting platform (42). A working hole (71) is opened on the front end surface of the optical path detection module (7). The working hole (71) is both a light source emission hole and a signal receiving hole. The temperature control module (6) includes a mounting base (61). A pair of symmetrically arranged L-shaped support plates (62) are mounted on the upper surface of the mounting base (61). The two support plates (62) are connected by a receiving end plate (63) at one end facing the optical path detection module (7). A light-transmitting hole (64) is opened on the receiving end plate (63). The light-transmitting hole (64) is coaxially arranged with the working hole (71). Heating pads (65) are provided on the opposite end faces of the two support plates (62). The detection chip (8) includes a transparent sample cell (81) and a support (82). The support (82) is disposed between a pair of support plates (62), and the sample cell (81) is disposed between a pair of heating pads (65).

2. The photoexcited chemiluminescence signal detection device as described in claim 1, characterized in that, The upper surface of the second mounting platform (42) is provided with a U-shaped mounting groove (43), the opening end of the mounting groove (43) faces the front end of the second mounting platform (42), the lower surface of the mounting base (61) is provided with a positioning hole, and the upper surface of the mounting groove (43) is provided with a positioning protrusion (44) corresponding to the positioning hole.

3. The photoexcited chemiluminescence signal detection device as described in claim 1, characterized in that, The detection chip (8) also includes a light guide plate (83) at the same height as the sample cell (81). Multiple horizontally arranged light guide grooves (84) are provided on the left and right side walls of the light guide plate (83). One end of the light guide plate (83) is fixedly connected to the side wall of the sample cell (81), and the other end of the light guide plate (83) is fixedly connected to the side wall of the support (82).

4. The photoexcited chemiluminescence signal detection device as described in claim 3, characterized in that, The left and right sidewalls of the sample cell (81) are outwardly convex arc-shaped structures, and the left and right sidewalls of the sample cell (81) are in contact with a pair of heating pads (65).

5. The photoexcited chemiluminescence signal detection device as described in claim 3, characterized in that, The sample cell (81) is a single-cell structure with a volume of 20-500 μl.

6. The photoexcited chemiluminescence signal detection device as described in claim 2, characterized in that, A U-shaped receiving opening (66) is provided on the mounting base (61) between the pair of support plates (62). A plug-in groove (67) is provided at the bottom of the receiving opening (66). The support body (82) includes a hand-held plate (821). The bottom of the hand-held plate (821) is integrally connected to the base (822). The bottom of the base (822) is provided with a plug-in protrusion (823). The plug-in protrusion (823) cooperates with the plug-in groove (67) to install the support body (82) into the receiving opening (66). The second mounting platform (42) is provided with a support platform (45) at its front end. The upper surface of the support platform (45) is on the same plane as the upper surface of the mounting groove (43), and the lower surface of the base (822) is in contact with the upper surface of the support platform (45).

7. The photoexcited chemiluminescence signal detection device as described in claim 1, characterized in that, The light-shielding front cover assembly (2) includes a fixed frame (21) and a light-shielding front cover (22) that are hinged to each other. The fixed frame (21) is installed at the front end of the main housing (1). An elastic U-shaped card plate (23) is installed on the top of the fixed frame (21). A pair of arc-shaped protrusions (24) are provided on the inner side of the front end of the U-shaped card plate (23). A locking plate (25) is installed on the top of the light-shielding front cover (22). The end of the locking plate (25) is an arc-shaped snap connector (26). The arc-shaped snap connector (26) cooperates with the U-shaped card plate (23) to fix the light-shielding front cover (22) to the fixed frame (21). A handle (27) is installed on the front end face of the light-shielding front cover (22).

8. The photoexcited chemiluminescence signal detection device as described in claim 1, characterized in that, The top of the receiving end plate (63) is higher than the top of the pair of support plates (62), and a temperature sensor (68) is installed on the side wall of the receiving end plate (63) facing the support plate (62).

9. The photoexcited chemiluminescence signal detection device as described in claim 1, characterized in that, A wiring box (9) is installed on the rear end face of the light-shielding back cover (3). An electronic wire bundle (10) is provided inside the wiring box (9). One end of the electronic wire bundle (10) passes through the light-shielding back cover (3) and is electrically connected to the excitation light source emitter (5), the optical path detection module (7), and the temperature control module (6).

10. The photoexcited chemiluminescence signal detection device as described in claim 7, characterized in that, The fixed frame (21) has grooves at both ends along the circumference, and a light-dense pad (11) is installed in the groove. The ends of the main shell (1) and the light-shielding front cover (22) are respectively inserted into the groove and abut against the light-dense pad (11).

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