Single-pool photoexcitation chemiluminescence detection chip
By combining a single-cell rectangular columnar sample cell with a curved reflective surface and a transparent light guide, the problems of cumbersome operation, poor portability, and insensitive signal acquisition of existing detection chips are solved, achieving efficient and accurate chemiluminescence detection.
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- FOURTH MILITARY MEDICAL UNIVERSITY
- Filing Date
- 2025-07-03
- Publication Date
- 2026-07-21
AI Technical Summary
Existing detection chips with multi-cell designs are cumbersome to operate, have poor portability, low signal acquisition sensitivity, are prone to leakage and have a high risk of false negatives, and traditional sample cells have low temperature control efficiency and high background noise.
The sample cell adopts a single-cell rectangular columnar structure, combined with curved sidewalls and reflective surface design, equipped with a transparent light guide and a high-reflectivity coated light guide groove, and uses a flexible cover to simplify operation and improve signal acquisition sensitivity and detection accuracy.
It achieves simple operation, low leakage rate, high portability, improved signal acquisition sensitivity and detection accuracy, reduced false negative risk, and reduced background noise by 80%.
Smart Images
Figure CN224535772U_ABST
Abstract
Description
Technical Field
[0001] This utility model belongs to the field of detection technology and relates to a detection chip, specifically a single-cell photoexcited chemiluminescence detection chip. Background Technology
[0002] Chemiluminescence immunoassay analyzer (CLIA) is a highly sensitive detection device that combines chemiluminescence technology with an immunoassay. It is primarily used for the quantitative or qualitative analysis of trace substances in clinical samples. As a crucial component of the chemiluminescence immunoassay analyzer, the existing detection chip suffers from the following problems:
[0003] 1. Most detection devices use multi-pool design for their detection chips. Although this is more efficient, the operation process is cumbersome and it is difficult to make them portable.
[0004] 2. Traditional sample cells have a right-angled facet design, which limits the heating area, resulting in low temperature control efficiency, narrow photosensitive area, and insufficient signal acquisition sensitivity.
[0005] 3. The excitation light is reflected in the sample cell, which increases the background noise and affects the accuracy of detection. In addition, excessive free photosensitive microspheres in the multi-cell design can cause random collisions, leading to the risk of false negatives.
[0006] 4. Existing detection chips lack ergonomic design, are prone to leakage during sample addition, and require auxiliary tools for operation. Summary of the Invention
[0007] In view of the shortcomings of the existing technology, the purpose of this utility model is to provide a single-cell photoexcited chemiluminescence detection chip, which can solve the technical problems of existing detection chips such as cumbersome operation, poor portability, low signal acquisition sensitivity, easy leakage and high risk of false negatives.
[0008] To solve the above-mentioned technical problems, the present invention adopts the following technical solution:
[0009] A single-cell photoexcited chemiluminescence detection chip includes a transparent sample cell, which is a rectangular columnar single-cell structure with an open top, and a support is connected to one of the transverse side walls of the sample cell.
[0010] The sample cell has vertically arranged reflective surfaces at the connection between two adjacent side walls, and both longitudinal side walls of the sample cell have outwardly convex arc-shaped structures; the support includes a handheld body connected to the sample cell, and a mounting base is fixedly connected to the bottom of the handheld body.
[0011] This utility model also includes the following technical features:
[0012] A transparent light guide is connected between the sample cell and the handheld body. The light guide includes a light guide plate and a connecting plate that are integrally connected vertically. The light guide plate is at the same height as the sample cell. Multiple equally spaced light guide grooves are opened on the two longitudinal side walls of the light guide plate. The two transverse ends of the light guide plate and the connecting plate are fixedly connected to the transverse side walls of the sample cell and the handheld body, respectively. A support protrusion is provided on the outer wall of the mounting base. The bottom of the connecting plate is fixedly installed on the upper surface of the support protrusion.
[0013] The angle between the two longitudinal sidewalls of the sample cell and its adjacent reflective surface is 45°.
[0014] The radii of curvature of the two longitudinal sidewalls of the sample cell are 0.1~0.3 mm.
[0015] The volume of the sample cell is 10~500μL.
[0016] The inner wall of the light guide groove is covered with a high reflectivity coating.
[0017] The light guide grooves are at least five in number.
[0018] The sample cell is detachably fitted with a flexible cap at its top.
[0019] The flexible cap is a TPU cap or a PP cap.
[0020] The handheld body has grip grooves machined on its two longitudinal side walls, and anti-slip textures are machined on the inner surface of the grip grooves.
[0021] Compared with the prior art, this utility model has the following technical effects:
[0022] This invention features a reasonable structure, simple operation, low leakage rate, high portability, and low cost. The single-cell, curved surface structure of the sample cell increases the heat-receiving area and photosensitive region of the sample cell, improves the sensitivity of signal acquisition, eliminates interference from free microspheres, reduces background noise by 80%, approaches zero false negative risk, and increases the accuracy of detection results. The structure of the light guide groove effectively guides the chemiluminescence signal to be transmitted in a directional manner, reduces background noise, and further improves detection accuracy. Attached Figure Description
[0023] Figure 1 This is a schematic diagram of the overall structure of this utility model.
[0024] Figure 2 This is a reference diagram showing the usage state of this utility model.
[0025] The meanings of the labels in the diagram are as follows:
[0026] 1. Sample cell, 2. Support, 3. Light guide, 4. Reflective surface, 5. Flexible cap, 21. Handheld body, 22. Mounting base, 23. Supporting flange, 24. Holding groove, 31. Light guide plate, 32. Connecting plate, 33. Light guide groove.
[0027] The specific content of this utility model will be further explained in detail below with reference to the embodiments. Detailed Implementation
[0028] Following the above technical solution, the following are specific embodiments of this utility model. It should be noted that this utility model is not limited to the following specific embodiments, and all equivalent modifications made based on the technical solution of this application fall within the protection scope of this utility model.
[0029] In this utility model, unless otherwise stated, directional terms such as "upper", "lower", "left", and "right" are generally defined based on the drawing in the corresponding figure; "inner" and "outer" refer to the inner and outer contours of the corresponding components; and "longitudinal", "transverse", and "vertical" refer to the directions marked in the figure.
[0030] Example:
[0031] This embodiment provides a single-cell photoexcited chemiluminescence detection chip, such as... Figures 1-2 As shown, it includes a transparent sample cell 1, which is a rectangular columnar single-cell structure with an open top, and a support part 2 is connected to one of the transverse side walls of the sample cell 1.
[0032] The connection between the two adjacent side walls of the sample cell 1 is provided with a vertically arranged reflective surface 4. The two longitudinal side walls of the sample cell 1 are both outwardly convex arc surface structures. The support part 2 includes a handheld body 21 connected to the sample cell, and a mounting base 22 is fixedly connected to the bottom of the handheld body 21.
[0033] In this embodiment, the detection chip is made of transparent PP material. The handheld body 21 facilitates sample addition and hand operation without the need for auxiliary tools. The mounting base 22 is used to install the detection chip into the corresponding part of the detection device. The single-cell sample cell 1 can eliminate background noise and hook effect in porous structures, improve detection accuracy, and is also suitable for small-volume detection devices, improving portability. In the photoexcitation chemiluminescence detection device, a heating device is provided to heat the sample in the sample cell 1. The radius of curvature of the arc structure of the two longitudinal sidewalls of the sample cell 1 is 0.1~0.3mm, which can increase the heated area of the sample cell 1 by 30%, reduce heat energy consumption, and at the same time, the photosensitive area can be expanded by 25%. The area of the sample in the sample cell 1 that is excited is increased, and the signal-to-noise ratio can be increased to 15:1, improving the sensitivity of signal acquisition.
[0034] In a preferred embodiment, a transparent light guide 3 connects the sample cell 1 and the handheld body 21. The light guide 3 includes a light guide plate 31 and a connecting plate 32 integrally connected vertically. The light guide plate 31 is at the same height as the sample cell 1. Multiple equally spaced light guide grooves 33 are formed on the two longitudinal sidewalls of the light guide plate 31. The transverse ends of the light guide plate 31 and the connecting plate 32 are fixedly connected to the transverse sidewalls of the sample cell 1 and the handheld body 21, respectively. In this embodiment, there are at least five light guide grooves 33. After the excitation light emitted by the excitation source passes through the sample cell 1, the light guide grooves 33... The excitation light that is not absorbed or scattered can be guided out along the light guide groove 33 to avoid affecting the efficiency of the optical path. The chemiluminescence signal generated after the sample is excited is in a scattered state. Some of the chemiluminescence signal is reflected when it hits the side wall of the light guide groove 33. The chemiluminescence signal can be directionally transmitted to the optical signal acquisition module and captured by it, reducing background noise, increasing signal strength, reducing the risk of false negatives, and improving detection accuracy. The outer wall of the mounting base 22 is provided with a support protrusion 23 in the horizontal direction. The bottom of the connecting plate 32 is fixedly installed on the upper surface of the support protrusion 23 to improve the structural stability of the detection chip.
[0035] Furthermore, the angle between the two longitudinal sidewalls of sample cell 1 and its adjacent reflective surface 4 is 45°, which can also help the excitation light signal to be directionally transmitted into the reaction chamber of sample cell 1, thereby increasing the excitation efficiency.
[0036] As a preferred embodiment, the volume of sample cell 1 in this embodiment is 10~500μL, preferably 20-200μL.
[0037] As a preferred embodiment, the inner wall of the light guide groove 33 is covered with a high reflectivity coating, which can reduce light scattering loss by 90%.
[0038] As a preferred embodiment, the top of the sample cell 1 is detachably fitted with a flexible cap 5, which is a TPU cap or a PP cap. After the sample is added, the flexible cap 5 is inserted into the top of the sample cell 1, which can reduce the leakage rate by more than 95%.
[0039] As a preferred embodiment, the two longitudinal sidewalls of the handheld body 21 are machined with grip grooves 24, and the inner surface of the grip grooves 24 is machined with anti-slip texture to improve the stability of single-handed grip.
[0040] In actual operation of this embodiment:
[0041] 1. Hold the handpiece 21 with one hand, add the sample into the sample cell 1, seal the sample cell 1 with the flexible cap 5, and install the detection chip into the corresponding position of the detection device;
[0042] 2. Turn on the excitation light source to excite the sample to generate a chemiluminescence signal;
[0043] 3. The reflective surface of the sample cell and the light guide groove are combined to directionally transmit the chemiluminescence signal to the light signal acquisition module. The background noise is eliminated by the filtering algorithm, and the detection result is output.
Claims
1. A single-cell photoexcited chemiluminescence detection chip, characterized in that, It includes a transparent sample cell (1), which is a rectangular columnar single-cell structure with an open top, and a support part (2) is connected to one of the transverse side walls of the sample cell (1). The sample pool (1) has vertically arranged reflective surfaces (4) at the connection of two adjacent side walls. The two longitudinal side walls of the sample pool (1) are outwardly convex arc structures. The support part (2) includes a handheld body (21) connected to the sample pool. The bottom of the handheld body (21) is fixedly connected to a mounting base (22).
2. The single-cell photoexcited chemiluminescence detection chip as described in claim 1, characterized in that, A transparent light guide (3) is connected between the sample cell (1) and the handheld body (21). The light guide (3) includes a light guide plate (31) and a connecting plate (32) that are integrally connected in the vertical direction. The light guide plate (31) is at the same height as the sample cell (1). Multiple light guide grooves (33) are provided in the horizontal direction on the two longitudinal side walls of the light guide plate (31). The horizontal ends of the light guide plate (31) and the connecting plate (32) are fixedly connected to the horizontal side walls of the sample cell (1) and the handheld body (21), respectively. A support protrusion (23) is provided in the horizontal direction on the outer wall of the mounting base (22). The bottom of the connecting plate (32) is fixedly installed on the upper surface of the support protrusion (23).
3. The single-cell photoexcited chemiluminescence detection chip as described in claim 1, characterized in that, The angle between the two longitudinal sidewalls of the sample cell (1) and its adjacent reflective surface (4) is 45°.
4. The single-cell photoexcited chemiluminescence detection chip as described in claim 1, characterized in that, The radius of curvature of the two longitudinal sidewalls of the sample cell (1) is 0.1~0.3 mm.
5. The single-cell photoexcited chemiluminescence detection chip as described in claim 1, characterized in that, The volume of the sample cell (1) is 10~500μL.
6. The single-cell photoexcited chemiluminescence detection chip as described in claim 2, characterized in that, The inner wall of the light guide groove (33) is covered with a high reflectivity coating.
7. The single-cell photoexcited chemiluminescence detection chip as described in claim 2, characterized in that, The light guide groove (33) is at least 5.
8. The single-cell photoexcited chemiluminescence detection chip as described in claim 1, characterized in that, The top of the sample cell (1) is detachably fitted with a flexible cap (5).
9. The single-cell photoexcited chemiluminescence detection chip as described in claim 8, characterized in that, The flexible cap (5) is a TPU cap or a PP cap.
10. The single-cell photoexcited chemiluminescence detection chip as described in claim 1, characterized in that, The handheld body (21) has grip grooves (24) machined on its two longitudinal side walls, and anti-slip textures are machined on the inner surface of the grip grooves (24).