LAMP closed tube detection device for milk powder detection

Abstract

The utility model relates to food detection technical field discloses a LAMP closed tube detection equipment for milk powder detection, including detector, the inside of detector contains sample processing module, constant temperature amplification module and optical detection module, the sample processing module contains sealed cabin, the inside of sealed cabin contains cutting head, negative pressure sample needle and pressure sensor, the constant temperature amplification module contains heating block, a plurality of temperature sensors and a plurality of reaction detection tubes, the optical detection module contains a plurality of LED light sources and diode receiver, the inside of detector is provided with waste collection module. In the utility model, the sample package is broken in the sealed cabin, the sample is transmitted through the sealed pipeline, the whole process closed detection from pretreatment to result interpretation of milk powder sample is realized, the open tube operation pollution risk in the LAMP reaction process is eliminated, and the detection precision and quality are improved.

Description

Technical Field

[0001] This utility model relates to the field of food testing technology, and in particular to a LAMP closed-tube testing device for milk powder testing. Background Technology

[0002] In the field of food quality and safety testing, milk powder, as an important source of nutrition for infants and special populations, is of paramount importance in terms of quality and safety. Loop-mediated isothermal amplification (LAMP) technology, with its advantages of not requiring complex temperature changes, rapid reaction speed, and high sensitivity, has shown great potential in detecting pathogenic microorganisms and genetically modified components in milk powder, becoming an important technical means to ensure milk powder quality and safety. Therefore, the usage rate of LAMP equipment for milk powder testing is increasing.

[0003] Current LAMP detection equipment mainly employs solutions such as open water baths combined with manual pipetting, semi-automatic microfluidic chip systems, or modified PCR instruments. Among these, the most commonly used open water baths rely on manual operation, requiring sample addition through pipetting. During the isothermal reaction at 60-65℃, the risk of aerosol contamination is extremely high, resulting in a false positive rate of up to 15%, which affects the accuracy and quality of the detection. Utility Model Content

[0004] To overcome the above shortcomings, this utility model provides a closed-tube LAMP detection device for milk powder detection, which aims to improve the problem that existing LAMP detection devices for milk powder require sample addition through open tube pipetting during the detection process, resulting in aerosol contamination of the sample during the reaction process, affecting the detection accuracy and quality.

[0005] To achieve the above objectives, this utility model provides the following technical solution: a LAMP closed-tube detection device for milk powder detection, comprising a detector, a terminal mounted on the outer wall of the detector, and an output terminal electrically connected to a sample processing module, an isothermal amplification module, an optical detection module, and a waste collection module. The sample processing module includes a sealed chamber, the interior of which includes a cutting head, a negative pressure sampling needle, and a pressure sensor. The isothermal amplification module includes a heating block, multiple temperature sensors, and multiple reaction detection tubes. The optical detection module includes multiple LED light sources and diode receivers. The waste collection module is located inside the detector.

[0006] The above technical solution involves sealing the entire testing process using a detector, controlling all modules via a terminal, pre-processing samples using a sample processing module, sealing the sample breaking process using a sealed chamber, automatically breaking open the sample packaging using a cutting head, drawing and transporting samples using negative pressure aspiration, maintaining sample temperature and automatically reacting using a constant-temperature amplification module, automatically detecting the reaction process in real time using an optical detection module, and automatically classifying and collecting waste materials after the reaction using a waste collection module. This eliminates the risk of contamination during tube opening operations in the LAMP reaction process, thereby ensuring testing accuracy and quality.

[0007] As a further description of the above technical solution: Preferably, the waste collection module includes a solenoid valve and a biosafety bag. The bottom of the biosafety bag is provided with a collection groove. The outer wall of the collection groove is slidably connected to the inner wall of the detector. Multiple locking blocks are fixedly connected to the outer wall of the collection groove. A slider is slidably connected to the inner wall of the detector. A pressing button and a connecting block are fixedly connected to the outer wall of the slider. A limit rod is fixedly connected to the inner wall of the detector. A spring is sleeved on the outer wall of the limit rod. Multiple sealing tubes are installed on the inner wall of the detector.

[0008] The above technical solution involves: installing biosafety bags inside the collection tank to safely store and treat reaction waste; connecting the collection tank to the detector by engaging a locking block on the collection tank with a slider inside the detector; and quickly disassembling the collection tank by pressing a button to release the locking block, facilitating rapid replacement of the biosafety bags. A sealing tube connects all components, ensuring a tight seal during the detection process.

[0009] As a further description of the above technical solution: Preferably, the bottom end of the sealed chamber is fixedly connected to the inner wall of the detector, the cutting head and the pressure sensor are both installed on the inner wall of the sealed chamber, and the sealed chamber and the negative pressure sampling needle are respectively located at both ends of the sealed tube.

[0010] The above technical solution ensures the sealing performance of the sample breaking process through a sealed chamber, automatically cuts the sample packaging through a cutting head, detects the cutting pressure in real time through a pressure sensor to facilitate the adjustment of the cutting pressure according to the packaging material, and uses a negative pressure suction needle to pick up the broken sample.

[0011] As a further description of the above technical solution: Preferably, the heating block is installed on the inner wall of the detector, the top of the heating block has multiple working holes, the temperature sensor is set at the bottom of the working holes, the reaction detection tube is installed on the inner wall of the working holes, and one of the sealing tubes is set at the output end of the reaction detection tube.

[0012] The above technical solution uses heating holes on the heating block to clamp and support the reaction detection tube, and continuously keeps the liquid inside the tube warm to ensure the stability of the reaction. A temperature sensor monitors the temperature in real time, facilitating automatic adjustment.

[0013] As a further description of the above technical solution: Preferably, the LED light source and the diode receiver are both installed on the inner wall of the detector and are respectively located on both sides of the heating block.

[0014] The above technical solution involves continuously scanning the reaction detection tube using an LED light source, detecting turbidity changes using a photodiode, and visualizing the detection data through a terminal.

[0015] As a further description of the above technical solution: Preferably, the solenoid valve is installed at the end of one of the sealing tubes, and the solenoid valve is located at the top of the collection tank.

[0016] The above technical solution involves installing a solenoid valve on the sealed tube to control the flow of waste, ensuring that it is sorted and placed into biosafety bags in the collection tank, thus preventing subsequent contamination.

[0017] As a further description of the above technical solution: Preferably, the inner wall of the connecting block is slidably connected to the outer wall of the limiting rod, one end of the spring is fixedly connected to the outer wall of the connecting block, and the other end of the spring is fixedly connected to the inner wall of the detector.

[0018] The above technical solution uses a limiting rod to limit the sliding of the connecting block, ensuring its stability and preventing deviation and tilting during the sliding process. A spring is used to allow the device to automatically reset after the user releases the handle.

[0019] As a further description of the above technical solution: Preferably, the inner wall of the detector is provided with multiple mounting holes, and the outer wall of the slider is provided with multiple engaging grooves, and the locking block is engaged with the inner wall of the mounting holes and engaging grooves.

[0020] The above technical solution uses mounting holes and locking slots to engage the card block, ensuring the stability of the collection tank when installed on the detector.

[0021] This utility model has the following beneficial effects: 1. In this utility model, by breaking open the sample packaging inside the sealed chamber and transferring the sample through a sealed pipe, a closed-loop detection process for milk powder samples from pretreatment to result interpretation is achieved, eliminating the risk of contamination during the opening of the tube in the LAMP reaction process and improving the detection accuracy and quality.

[0022] 2. In this utility model, by installing a locking block on the waste collection tank, pressing the button drives the slider and the connecting block to slide synchronously, so that the locking groove on the slider releases the locking block, allowing the waste collection tank to be quickly disassembled and the internal biosafety bag to be quickly replaced, further improving the detection efficiency. Attached Figure Description

[0023] Figure 1 This is a perspective view of a LAMP closed-tube detection device for milk powder testing proposed in this utility model; Figure 2 This is a cross-sectional view of the internal structure of a LAMP closed-tube detection device for milk powder testing proposed in this utility model; Figure 3 This is a rear view of a LAMP closed-tube detection device for milk powder testing proposed in this utility model; Figure 4 This is a separate schematic diagram of the waste collection module of a LAMP closed-tube detection device for milk powder testing proposed in this utility model. Figure 5 This is a cross-sectional view of the waste collection module of a LAMP closed-tube detection device for milk powder testing proposed in this utility model; Figure 6 This is a schematic block diagram of the overall structure of a LAMP closed-tube detection device for milk powder testing proposed in this utility model; Figure 7 This is a schematic block diagram of the sample processing module of a LAMP closed-tube detection device for milk powder detection proposed in this utility model; Figure 8 This is a schematic block diagram of the isothermal amplification module of a LAMP closed-tube detection device for milk powder detection proposed in this utility model; Figure 9 This is a schematic block diagram of the optical detection module of a LAMP closed-tube detection device for milk powder detection proposed in this utility model; Figure 10 This is a schematic block diagram of the waste collection module of a LAMP closed-tube detection device for milk powder testing proposed in this utility model; Figure 11 This is a schematic block diagram of the terminal control structure of a LAMP closed-tube detection device for milk powder testing proposed in this utility model.

[0024] Legend: 1. Detector; 2. Sample processing module; 3. Isothermal amplification module; 4. Optical detection module; 5. Sealed chamber; 6. Cutting head; 7. Negative pressure sampling needle; 8. Pressure sensor; 9. Heating block; 10. Temperature sensor; 11. Reaction detection tube; 12. LED light source; 13. Diode receiver; 14. Waste collection module; 1401. Solenoid valve; 1402. Biosafety bag; 1403. Collection tank; 1404. Locking block; 1405. Press button; 1406. Slider; 1407. Connecting block; 1408. Limiting rod; 1409. Spring; 15. Sealing tube; 16. Terminal. Detailed Implementation

[0025] The technical solutions of the embodiments of this utility model will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of this utility model, and not all embodiments. Based on the embodiments of this utility model, all other embodiments obtained by those skilled in the art without creative effort are within the protection scope of this utility model.

[0026] Reference Figure 1 , Figure 6 and Figure 11 The present invention provides an embodiment of a closed-tube LAMP detection device for milk powder detection, comprising a detector 1, a terminal 16 mounted on the outer wall of the detector 1, and an output terminal of the terminal 16 electrically connected to a sample processing module 2, an isothermal amplification module 3, an optical detection module 4, and a waste collection module 14. The sample processing module 2 includes a sealed chamber 5, and the interior of the sealed chamber 5 includes a cutting head 6, a negative pressure sampling needle 7, and a pressure sensor 8. The isothermal amplification module 3 includes a heating block 9, multiple temperature sensors 10, and multiple reaction detection tubes 11. The optical detection module 4 includes multiple LED light sources 12 and diode receivers 13. The waste collection module 14 is disposed inside the detector 1. Specifically, the main body of the detector 1 is 600×500×400mm, the weight of the whole machine is 28kg, it uses 220V power supply, and the maximum power consumption is 800W. The entire detector 1 is sealed and mainly includes sample processing module 2, isothermal amplification module 3, optical detection module 4 and waste collection module 14. It realizes a closed detection process from pretreatment to result interpretation, thereby eliminating the risk of contamination from tube opening operation in the LAMP reaction process. The terminal 16 controls the linkage between multiple modules to perform automatic detection and result display, thereby improving detection efficiency and quality.

[0027] Reference Figure 3 , Figure 4 and Figure 5The waste collection module 14 includes a solenoid valve 1401 and a biosafety bag 1402. A collection trough 1403 is provided at the bottom of the biosafety bag 1402. The outer wall of the collection trough 1403 is slidably connected to the inner wall of the detector 1. Multiple locking blocks 1404 are fixedly connected to the outer wall of the collection trough 1403. A slider 1406 is slidably connected to the inner wall of the detector 1. A pressing button 1405 and a connecting block 1407 are fixedly connected to the outer wall of the slider 1406. A limit rod 1408 is fixedly connected to the inner wall of the detector 1. A spring 1409 is sleeved on the outer wall of the limiting rod 1408. Multiple sealing tubes 15 are installed on the inner wall of the detector 1. The inner wall of the connecting block 1407 is slidably connected to the outer wall of the limiting rod 1408. One end of the spring 1409 is fixedly connected to the outer wall of the connecting block 1407, and the other end of the spring 1409 is fixedly connected to the inner wall of the detector 1. Multiple mounting holes are opened on the inner wall of the detector 1. Multiple engaging grooves are opened and penetrated on the outer wall of the slider 1406. The locking block 1404 is engaged in the inner wall of the mounting holes and engaging grooves. Specifically, the waste collection module 14 is equipped with a dual-chamber biosafety bag 1402. The flow of waste liquid is controlled by a solenoid valve 1401. The biosafety bag 1402 is fitted onto the upper end of a collection tank 1403 with multiple slots. Multiple locking blocks 1404 installed on the collection tank 1403 are connected to the locking slots and mounting holes installed inside the detector 1 for quick disassembly and replacement. Pressing the button 1405 on the outer wall of the detector 1 causes the slider 1406 to slide inwards synchronously. A connecting block 1 is installed on the outer wall of the slider 1406. During the sliding process, 407 always follows the outer wall of the limiting rod 1408 to limit the sliding direction. At the same time, the spring 1409 is compressed. When pressed to the bottom, the locking groove on the slider 1406 will align with the mounting hole on the detector 1 and be on the same straight line, releasing the locking of the locking block 1404. This allows the collection slot 1403 to be pulled out freely, so that the biosafety bag 1402 on top can be replaced. The stress of the spring 1409 allows the device to automatically reset after being pulled out, thereby increasing the convenience of the device.

[0028] Reference Figure 2 and Figure 7 The bottom of the sealed chamber 5 is fixedly connected to the inner wall of the detector 1. The cutting head 6 and the pressure sensor 8 are both installed on the inner wall of the sealed chamber 5. The sealed chamber 5 and the negative pressure sampling needle 7 are respectively set at both ends of the sealed tube 15. Specifically, the entire sample processing process takes place inside the sealed chamber 5. First, the sample packaging to be tested is placed inside the sealed chamber 5. After the chamber door is closed, the laser cutting head 6 is controlled by the terminal 16 to break open the sample packaging. Then, the negative pressure aspiration needle 7 draws up the sample under a negative pressure of -80 kPa and passes it through the sealed tube 15 into the subsequent isothermal amplification module 3 for testing. The pressure sensor 8 is used to detect and identify the puncture and cutting resistance of different packaging such as iron cans / aluminum foil bags.

[0029] Reference Figure 2 and Figure 8 The heating block 9 is installed on the inner wall of the detector 1. Multiple working holes are opened at the top of the heating block 9. The temperature sensor 10 is set at the bottom of the working hole. The reaction detection tube 11 is installed on the inner wall of the working hole. One of the sealing tubes 15 is set at the output end of the reaction detection tube 11. Specifically, the isothermal amplification module 3 includes a 96-well aluminum heating block 9, with a PT100 temperature sensor 10 integrated at the bottom of each working well. The heating block 9 is made of 6061 aluminum alloy CNC machined and anodized. A reaction detection tube 11 pre-filled with reaction solution is installed in the working well. During the detection process, the heating block 9 continuously heats the reaction detection tube 11 to maintain a constant temperature of 63±0.5℃ for LAMP reaction. The reaction detection tube 11 uses a double-layer sealing cap, so that the sample drawn by the negative pressure sampling needle 7 automatically returns to the sealed state after puncture and injection. The sample is injected through the sealing tube 15.

[0030] Reference Figure 1 , Figure 2 and Figure 9 The LED light source 12 and the diode receiver 13 are both installed on the inner wall of the detector 1 and are respectively located on both sides of the heating block 9; Specifically, the optical detection module 4 consists of a 470nm LED light source array 12 and a photodiode receiver 13. The light source and receiver are arranged at a 45° angle. The LED light source 12 scans the reaction tube every 30 seconds, and the photodiode receiver 13 detects the turbidity change. The photodiode is selected from the Hamamatsu S1223-01 series, with a response wavelength range of 400-600nm. Quantitative analysis of amplification products is achieved through in-situ optical detection, and the real-time amplification curve is displayed on the terminal 16. The detection result is automatically determined by the built-in algorithm to determine the threshold cycle number Ct value. A positive result is indicated by a red audible and visual alarm.

[0031] Reference Figure 4 and Figure 10 Solenoid valve 1401 is installed at the end of one of the sealing tubes 15, and solenoid valve 1401 is located at the top of the collection tank 1403. Specifically, multiple sealing gaskets are installed on both the sealing tube 15 and the solenoid valve 1401. The sealing gaskets are made of medical-grade silicone rubber with a Shore hardness of 50±5, which further ensures the sealing performance during the testing process. After the reaction is completed, the waste liquid will enter the sealing tube 15 and be automatically diverted into the corresponding biosafety bag 1402 for classified storage under the control of the solenoid valve 1401 to prevent subsequent contamination.

[0032] Working principle: When using this device to test milk powder, the unopened milk powder package is placed into the sealed chamber 5 and the safety door is closed. The corresponding test item is selected on the terminal 16, and the laser cutting head 6 is started to automatically break open the milk powder package. After breaking open, the negative pressure sampling needle 7 draws the sample under the negative pressure of the sealed chamber 5 and transfers the sample to the reaction detection tube 11, which has been injected with pre-filled reaction solution, through the sealed tube 15. The heating block 9 continuously heats the reaction detection tube 11 to maintain a constant temperature for LAMP reaction. The LED light source 12 continuously scans the reaction detection tube 11, and the diode receiver 13 detects the turbidity change inside the reaction detection tube 11 in real time. After the reaction is completed, the waste liquid is automatically discharged into the biosafety bag 1402 in the corresponding collection tank 1403 after being controlled by the solenoid valve 1401 through the sealed tube 15, waiting for subsequent processing. After the reaction cycle is complete, you can view the real-time amplification curve displayed on terminal 16; After the test is completed, manually remove the remaining packaging from the sealed chamber 5 and discard it. Then, manually press the button 1405 inward to make the slider 1406 and the connecting block 1407 slide together. While compressing the spring 1409, the locking groove on the slider 1406 is aligned with the mounting hole on the detector 1, releasing the locking block 1404 installed on the collection tank 1403. At this time, the collection tank 1403 can be quickly disassembled by pulling the handle on the outside of the collection tank 1403, and the biosafety bag 1402 inside can be disassembled and replaced.

[0033] Finally, it should be noted that the above description is only a preferred embodiment of the present utility model and is not intended to limit the present utility model. Although the present utility model has been described in detail with reference to the foregoing embodiments, those skilled in the art can still modify the technical solutions described in the foregoing embodiments or make equivalent substitutions for some of the technical features. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of the present utility model should be included within the protection scope of the present utility model.

Claims

1. A closed-tube LAMP detection device for milk powder testing, comprising a detector (1), characterized in that: The outer wall of the detector (1) is equipped with a terminal (16). The output end of the terminal (16) is electrically connected to the sample processing module (2), the isothermal amplification module (3), the optical detection module (4), and the waste collection module (14). The sample processing module (2) includes a sealed chamber (5). The interior of the sealed chamber (5) includes a cutting head (6), a negative pressure sampling needle (7), and a pressure sensor (8). The isothermal amplification module (3) includes a heating block (9), multiple temperature sensors (10), and multiple reaction detection tubes (11). The optical detection module (4) includes multiple LED light sources (12) and diode receivers (13). The waste collection module (14) is located inside the detector (1).

2. The LAMP closed-tube detection device for milk powder detection according to claim 1, characterized in that: The waste collection module (14) includes a solenoid valve (1401) and a biosafety bag (1402). The bottom end of the biosafety bag (1402) is provided with a collection groove (1403). The outer wall of the collection groove (1403) is slidably connected to the inner wall of the detector (1). The outer wall of the collection groove (1403) is fixedly connected with multiple locking blocks (1404). The inner wall of the detector (1) is slidably connected with a slider (1406). The outer wall of the slider (1406) is fixedly connected with a pressing button (1405) and a connecting block (1407). The inner wall of the detector (1) is fixedly connected with a limiting rod (1408). The outer wall of the limiting rod (1408) is fitted with a spring (1409). The inner wall of the detector (1) is equipped with multiple sealing tubes (15).

3. The LAMP closed-tube detection device for milk powder detection according to claim 2, characterized in that: The bottom end of the sealed chamber (5) is fixedly connected to the inner wall of the detector (1). The cutting head (6) and the pressure sensor (8) are both installed on the inner wall of the sealed chamber (5). The sealed chamber (5) and the negative pressure sampling needle (7) are respectively set at both ends of the sealed tube (15).

4. The LAMP closed-tube detection device for milk powder detection according to claim 2, characterized in that: The heating block (9) is installed on the inner wall of the detector (1). The top of the heating block (9) has multiple working holes. The temperature sensor (10) is located at the bottom of the working hole. The reaction detection tube (11) is installed on the inner wall of the working hole. One of the sealing tubes (15) is located at the output end of the reaction detection tube (11).

5. The LAMP closed-tube detection device for milk powder detection according to claim 1, characterized in that: The LED light source (12) and diode receiver (13) are both installed on the inner wall of the detector (1) and respectively set on both sides of the heating block (9).

6. The LAMP closed-tube detection device for milk powder detection according to claim 2, characterized in that: The solenoid valve (1401) is installed at the end of one of the sealing tubes (15) and is located at the top of the collection tank (1403).

7. The LAMP closed-tube detection device for milk powder detection according to claim 2, characterized in that: The inner wall of the connecting block (1407) is slidably connected to the outer wall of the limiting rod (1408), one end of the spring (1409) is fixedly connected to the outer wall of the connecting block (1407), and the other end of the spring (1409) is fixedly connected to the inner wall of the detector (1).

8. The LAMP closed-tube detection device for milk powder detection according to claim 2, characterized in that: The inner wall of the detector (1) is provided with multiple mounting holes, and the outer wall of the slider (1406) is provided with multiple engaging grooves. The locking block (1404) is engaged with the inner wall of the mounting holes and engaging grooves.

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