Portable microfluidic gene rapid detection chip device

Abstract

The utility model discloses a portable micro -fluidic gene rapid detection chip device relates to gene detection technical field, including the rotation setting micro -fluidic chip, is provided with a plurality of groups by sample pool, siphon passage and arc channel and is connected gradually to constitute the liquid flow channel of liquid flow channel on the micro -fluidic chip, a plurality of buffer channels are evenly passed through and set up on the arc channel, this scheme is through the special arc structure design, and the stable circulation is formed in buffer pipe no.

Description

Technical Field

[0001] This utility model relates to the technical field of gene detection, specifically a portable microfluidic gene rapid detection chip device. Background Technology

[0002] With the continuous development of biotechnology, gene detection plays a vital role in many fields such as medical diagnosis, biological research, and food safety. However, existing microfluidic chips still have many shortcomings in controlling sample liquid flow. For example, during the process of chip rotation driving liquid flow, the high-speed rotating chip can impact the buffer channel and connecting pipes before the liquid enters the reaction cell. The liquid is also prone to unstable flow due to factors such as sudden changes in centrifugal force and unreasonable channel structure.

[0003] Therefore, those skilled in the art have provided portable microfluidic gene rapid detection chip devices to address the problems raised. Utility Model Content

[0004] To address the aforementioned problems, this invention provides a portable microfluidic gene rapid detection chip device.

[0005] To achieve the above objectives, the technical solution of this utility model is as follows:

[0006] A portable microfluidic gene rapid detection chip device includes a rotatable microfluidic chip. The microfluidic chip is provided with multiple sets of liquid flow channels, which are composed of a sample cell, a siphon channel and an arc-shaped channel connected in sequence. Multiple buffer channels are uniformly arranged through the arc-shaped channel.

[0007] The buffer channel includes buffer tube one and buffer tube two with their opening ends facing the rotation direction of the microfluidic chip. The tangents at the inlet ends of buffer tube one and buffer tube two coincide with the tangent direction at the connection point of the arc-shaped channel. The inlet end of buffer tube one is closer to the connection point of the siphon channel and the arc-shaped channel than the inlet end of buffer tube two.

[0008] The buffer tube is provided with a first arc-shaped part and a second arc-shaped part. The outer arc surface of the first arc-shaped part and the inner arc surface of the second arc-shaped part are both facing the second buffer tube. The end of the second arc-shaped part away from the arc-shaped channel is provided with an outlet pipe and a flushing pipe for diversion.

[0009] The second buffer tube is provided with a third arc-shaped part with its inner arc surface facing the first buffer tube. The end of the third arc-shaped part away from the arc-shaped channel is provided with a second flushing tube and a second outlet tube for diversion.

[0010] The first and second flushing pipes are connected in a continuous manner, and both the first and second liquid outlet pipes are connected to the reaction tank through capillary tubes.

[0011] Preferably, the length from the inlet end of the first buffer tube to the end of the first outlet tube away from the second arc-shaped portion is equal to the length from the inlet end of the second buffer tube to the end of the second outlet tube away from the third arc-shaped portion.

[0012] Preferably, the tangent directions at the connection point of the inner arc surfaces of the first and second arc-shaped sections coincide, and the tangent directions at the connection point of the outer arc surfaces of the first and second arc-shaped sections coincide.

[0013] Preferably, the tangent directions at the connection point of the second flushing pipe and the inner arc surface of the third arc section coincide, and the tangent directions at the connection point of the second liquid outlet pipe and the outer arc surface of the third arc section coincide.

[0014] Preferably, the inner diameter of the second anti-flush tube is the same as that of the first anti-flush tube, and the central axis of the second anti-flush tube coincides with that of the first anti-flush tube.

[0015] Preferably, the branching point of the first outlet pipe and the first flushing pipe is located at the middle position of the end facing the second arc-shaped portion; the branching point of the second flushing pipe and the second outlet pipe is located at the middle position of the end facing the third arc-shaped portion.

[0016] In summary, this utility model has the following beneficial technical effects:

[0017] The first buffer tube is provided with a first arc-shaped section and a second arc-shaped section. The outer arc surface of the first arc-shaped section and the inner arc surface of the second arc-shaped section both face the second buffer tube. Through the special arc-shaped structure design, the liquid is guided to form a stable circulation in the first buffer tube. The second buffer tube is provided with a third arc-shaped section with its inner arc surface facing the first buffer tube. Similarly, the arc-shaped structure is used to buffer and guide the incoming liquid, reduce the flow velocity and buffer the pressure. The first and second flushing tubes are connected to form a liquid flushing structure, which can effectively counteract the uneven liquid flow velocity caused by centrifugal force and other factors, stabilize the flow field, and protect the relevant structures. Attached Figure Description

[0018] The disclosure of this utility model is illustrated with reference to the accompanying drawings. It should be understood that the drawings are for illustrative purposes only and are not intended to limit the scope of protection of this utility model. In the drawings, the same reference numerals are used to refer to the same parts. Wherein:

[0019] Figure 1 This is a schematic diagram of the overall cross-sectional structure of this utility model;

[0020] Figure 2 This is a schematic diagram of the surface structure of the microfluidic chip of this utility model;

[0021] Figure 3 This is a schematic diagram of the liquid flow channel structure of this utility model;

[0022] Figure 4 for Figure 3An enlarged schematic diagram of region A in the diagram.

[0023] Explanation of reference numerals in the attached diagram: 1. Microfluidic chip; 2. Buffer tube one; 3. Buffer tube two; 11. Sample dispensing cell; 12. Siphon channel; 13. Arc-shaped channel; 14. Capillary; 15. Reaction cell; 21. First arc-shaped section; 22. Second arc-shaped section; 23. Outlet tube one; 24. Flushing tube one; 31. Third arc-shaped section; 32. Flushing tube two; 33. Outlet tube two. Detailed Implementation

[0024] It is readily understood that, based on the technical solution of this utility model, those skilled in the art can propose various interchangeable structural methods and implementations without altering the essential spirit of this utility model. Therefore, the following detailed embodiments and accompanying drawings are merely illustrative descriptions of the technical solution of this utility model and should not be considered as the entirety of this utility model or as limitations or restrictions on the technical solution of this utility model.

[0025] A portable microfluidic gene rapid detection chip device includes a rotating microfluidic chip 1, which can be driven by a motor. The microfluidic chip 1 is provided with multiple sets of liquid flow channels, which are sequentially connected by a sample loading cell 11, a siphon channel 12, and an arc-shaped channel 13. Multiple buffer channels are uniformly arranged on the arc-shaped channel 13. In use, the gene sample to be detected is first added to the sample loading cell 11, and then the rotation device of the microfluidic chip 1 is started. The microfluidic chip 1 begins to rotate at a constant speed around its axis. Under the action of centrifugal force, the sample liquid quickly enters the arc-shaped channel 13 through the siphon channel 12, and then fills the buffer channels. The excess liquid enters the waste liquid pool.

[0026] The microfluidic chip 1 is also equipped with an exhaust pipe. One end of the exhaust pipe is connected to the sample filling cell 11, and the other end of the exhaust pipe is connected to the outlet end of the arc-shaped channel 13. By setting the exhaust pipe, the air pressure in the sample filling cell 11 and the arc-shaped channel 13 is balanced, which makes it easier for the liquid in the arc-shaped channel 13 to fill multiple buffer channels in sequence.

[0027] The microfluidic chip 1, its connection structure, and related auxiliary structures are externally equipped with a housing, which makes it easy to carry as a whole.

[0028] The buffer channel includes buffer tube 2 and buffer tube 3, with their openings facing the rotation direction of the microfluidic chip 1. The tangents at the inlet ends of buffer tube 2 and buffer tube 3 coincide with the tangent at the connection point of the arc-shaped channel 13, ensuring that the liquid can flow in smoothly. The inlet end of buffer tube 2 is closer to the connection point of the siphon channel 12 and the arc-shaped channel 13 than the inlet end of buffer tube 3, which is beneficial for the graded buffering and diversion of the inflowing liquid.

[0029] The buffer tube 2 is provided with a first arc-shaped part 21 and a second arc-shaped part 22. The outer arc surface of the first arc-shaped part 21 and the inner arc surface of the second arc-shaped part 22 are both facing the buffer tube 3. Through the special arc-shaped structure design, the liquid is guided to form a stable circulation in the buffer tube 2, reducing the flow rate and buffering the pressure. The end of the second arc-shaped part 22 away from the arc-shaped channel 13 is provided with an outlet pipe 23 and a flushing pipe 24 to divert the flow, so that the liquid can flow to the subsequent stage according to the predetermined path.

[0030] The buffer tube 2 3 is provided with a third arc-shaped part 31 with its inner arc surface facing the buffer tube 1 2. The arc-shaped structure is used to buffer and guide the incoming liquid. The end of the third arc-shaped part 31 away from the arc-shaped channel 13 is provided with a flushing tube 2 32 and an outlet tube 2 33 for diversion.

[0031] The first counter-flushing tube 24 and the second counter-flushing tube 32 are connected to form a liquid counter-flushing structure, which can effectively counteract the uneven liquid flow velocity caused by centrifugal force and other factors, and stabilize the flow field. Furthermore, the inner diameters of the second counter-flushing tube 32 and the first counter-flushing tube 24 are equal, and the central axes of the second counter-flushing tube 32 and the first counter-flushing tube 24 coincide, ensuring the uniformity and symmetry of the counter-flushing effect, and ensuring that the liquid energy cancels each other out during the counter-flushing process, resulting in a uniform and stable flow velocity.

[0032] Both outlet pipe 23 and outlet pipe 33 are connected to the reaction tank 15 via capillary tube 14, ensuring that the liquid after buffering and diversion treatment enters the reaction tank 15 accurately and stably for gene detection reaction. The liquid velocity after deceleration is relatively low, which can protect the capillary tube 14.

[0033] Furthermore, the length from the inlet end of buffer tube 2 to the end of outlet tube 23 away from the second arc-shaped section 22 is equal to the length from the inlet end of buffer tube 3 to the end of outlet tube 33 away from the third arc-shaped section 31. This ensures that the liquid flows for similar times on the two diversion paths, avoiding the problem of sequential arrival due to path differences, and further improving the synchronicity and stability of liquid delivery.

[0034] The tangents at the connection points of the inner arc surfaces of the first flushing pipe 24 and the second arc-shaped section 22 coincide, the tangents at the connection points of the first liquid outlet pipe 23 and the outer arc surfaces of the second arc-shaped section 22 coincide, the tangents at the connection points of the second flushing pipe 32 and the inner arc surfaces of the third arc-shaped section 31 coincide, and the tangents at the connection points of the second liquid outlet pipe 33 and the outer arc surfaces of the third arc-shaped section 31 coincide. These precise tangent design features make the liquid flow more smoothly during diversion and turning, reduce turbulence, and maintain a stable flow state.

[0035] The branching points of the first outlet pipe 23 and the first flushing pipe 24 are located at the middle position of the end of the second arc-shaped section 22; the branching points of the second flushing pipe 32 and the second outlet pipe 33 are located at the middle position of the end of the third arc-shaped section 31. This branching position design is conducive to balancing the liquid flow in each branch pipe and improving the stability of the overall flow path.

[0036] The overall device has a compact structure, and multiple sets of liquid flow channels and buffer components can work in parallel, which greatly shortens the detection time.

[0037] The technical scope of this utility model is not limited to the content described above. Those skilled in the art can make various modifications and variations to the above embodiments without departing from the technical concept of this utility model, and all such modifications and variations should fall within the protection scope of this utility model.

Claims

1. A portable microfluidic gene rapid detection chip device, comprising a rotatably mounted microfluidic chip (1), wherein the microfluidic chip (1) is provided with multiple sets of fluid flow channels sequentially connected by a sample loading cell (11), a siphon channel (12), and an arc-shaped channel (13), characterized in that: Multiple buffer channels are uniformly arranged on the arc-shaped channel (13); The buffer channel includes a buffer tube 1 (2) and a buffer tube 2 (3) with their opening ends facing the rotation direction of the microfluidic chip (1). The tangents at the inlet ends of the buffer tube 1 (2) and the buffer tube 2 (3) coincide with the tangent direction at the connection point of the arc channel (13). The inlet end of the buffer tube 1 (2) is closer to the connection point of the siphon channel (12) and the arc channel (13) than the inlet end of the buffer tube 2 (3). The buffer tube 1 (2) is provided with a first arc-shaped part (21) and a second arc-shaped part (22). The outer arc surface of the first arc-shaped part (21) and the inner arc surface of the second arc-shaped part (22) are both facing the buffer tube 2 (3). The end of the second arc-shaped part (22) away from the arc-shaped channel (13) is provided with an outlet pipe 1 (23) and a flushing pipe 1 (24) for diversion. The buffer tube 2 (3) is provided with a third arc-shaped part (31) with its inner arc surface facing the buffer tube 1 (2). The end of the third arc-shaped part (31) away from the arc-shaped channel (13) is provided with a flushing tube 2 (32) and an outlet tube 2 (33) for diversion. The first flushing pipe (24) and the second flushing pipe (32) are connected in a continuous manner, and both the first liquid outlet pipe (23) and the second liquid outlet pipe (33) are connected to the reaction tank (15) through the capillary tube (14).

2. The portable microfluidic gene rapid detection chip device according to claim 1, characterized in that: The length from the inlet end of the first buffer tube (2) to the end of the first outlet tube (23) away from the second arc-shaped part (22) is equal to the length from the inlet end of the second buffer tube (3) to the end of the second outlet tube (33) away from the third arc-shaped part (31).

3. The portable microfluidic gene rapid detection chip device according to claim 2, characterized in that: The tangents at the connection point of the inner arc surface of the first flushing pipe (24) and the second arc-shaped part (22) coincide, and the tangents at the connection point of the outer arc surface of the first liquid outlet pipe (23) and the second arc-shaped part (22) coincide.

4. The portable microfluidic gene rapid detection chip device according to claim 3, characterized in that: The tangents at the connection point of the second flushing pipe (32) and the inner arc surface of the third arc part (31) coincide, and the tangents at the connection point of the second liquid outlet pipe (33) and the outer arc surface of the third arc part (31) coincide.

5. The portable microfluidic gene rapid detection chip device according to claim 4, characterized in that: The inner diameter of the second anti-flush tube (32) is equal to that of the first anti-flush tube (24), and the central axis of the second anti-flush tube (32) coincides with that of the first anti-flush tube (24).

6. The portable microfluidic gene rapid detection chip device according to claim 5, characterized in that: The branching points of the first outlet pipe (23) and the first flushing pipe (24) are located at the middle position of the end facing the second arc-shaped part (22); the branching points of the second flushing pipe (32) and the second outlet pipe (33) are located at the middle position of the end facing the third arc-shaped part (31).

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