Anti-contamination cell culture dish
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- SICHUAN AGRI UNIV
- Filing Date
- 2025-08-28
- Publication Date
- 2026-07-24
AI Technical Summary
Traditional cell culture dishes have insufficient sealing, which can easily lead to culture medium evaporation and microbial contamination. Gas exchange is uncontrollable, affecting cell metabolism. Furthermore, the breathable membrane is inconvenient to install and prone to leakage, resulting in a high contamination rate.
The device employs a threaded connection between the vessel body and the sealing cap to form a double seal. Combined with the vent holes on the sealing cap and the circular filter membrane, a threaded connector is used to fix the pressure ring to tightly compress the filter membrane, achieving gas exchange and multi-layer sealing, thus reducing the risk of contamination.
It achieves double sealing of cell culture dishes, reduces the risk of culture medium evaporation and microbial contamination, ensures controllable gas exchange, and facilitates filter membrane replacement and reuse of culture dishes.
Smart Images
Figure CN224548414U_ABST
Abstract
Description
Technical Field
[0001] This utility model relates to the field of cell culture dish technology, specifically to a contamination-resistant cell culture dish. Background Technology
[0002] During cell culture, the sealing performance of the culture dish directly affects the stability of the cell growth environment. Traditional culture dishes mainly use a simple lid-locking method, which has the following defects: (1) Insufficient sealing, which easily leads to culture medium evaporation and microbial contamination; (2) Uncontrollable gas exchange, which affects cell metabolism; (3) Repeated opening and closing can easily cause sealing failure.
[0003] Currently, improvements are commonly made by using single-layer silicone gaskets for sealing or by setting up breathable membranes. However, these methods still suffer from problems such as easy leakage at the edges and a lack of reliable installation and locking structures for the breathable membranes, making them prone to leakage and difficult to replace. In particular, during long-term cultivation, the contamination rate of traditional culture dishes can reach as high as 5-15%, which seriously affects the reliability of experimental results. Utility Model Content
[0004] To address the aforementioned shortcomings of existing technologies, this invention provides a contamination-resistant cell culture dish, solving the problem of easy contamination in existing cell culture dishes.
[0005] To achieve the above objectives, the technical solution adopted by this utility model is as follows: A contamination-resistant cell culture dish is provided, comprising a dish body and a sealing cap. Both the dish body and the sealing cap are cylindrical with one open end. A first external thread is provided on the outer side wall of the open end of the dish body, and a first internal thread is provided on the inner side wall of the open end of the sealing cap to mate with the first external thread. A vent hole is provided in the middle of the non-open end of the sealing cap, and a circular filter membrane covering the vent hole is provided on the sealing cap. The circular filter membrane is detachably fixed to the sealing cap by a sealing connector.
[0006] Furthermore, the sealing connector includes a pressure ring, the outer edge of which is connected to one end of the sleeve. The inner wall of the sleeve is provided with a second internal thread, and the sealing cover is provided with an annular groove. The inner wall of the annular groove is provided with a second external thread that mates with the second internal thread. The pressure ring presses the circular filter membrane tightly against the surface of the sealing cover.
[0007] Furthermore, the annular groove and the vent hole are concentrically arranged, the radial dimension of the circular filter membrane is larger than the radial dimension of the vent hole and smaller than the radial dimension of the outer circle of the pressure ring, and the radial dimension of the inner circle of the pressure ring is not smaller than the radial dimension of the vent hole.
[0008] Furthermore, several sealing rings of different sizes are concentrically arranged on the annular end face of the pressure ring that contacts the circular filter membrane.
[0009] Furthermore, several vertical stripes are provided on the outer wall of the pressure ring.
[0010] Furthermore, an annular sealing strip is provided on the inner edge of the non-open end of the sealing cap, and an annular groove is provided on the end face of the annular sealing strip for abutting the open end of the vessel body. The cross-section of the annular groove is in the shape of an isosceles trapezoid.
[0011] Furthermore, the circular filter membrane is a hydrophobic polytetrafluoroethylene filter membrane with a thickness of 0.22 μm.
[0012] The beneficial effects of this utility model are as follows: 1. This solution forms the first seal inside the dish body through the threaded engagement of the dish body and the sealing cap. At the same time, the open end of the dish body extends into the annular groove on the annular sealing strip, and the annular sealing strip is squeezed under the force of the threaded engagement to form the second seal inside the dish body. This achieves a double seal inside the dish body, reducing the risk of culture medium evaporation and microbial contamination.
[0013] 2. This method achieves CO2 / O2 exchange between the inside of the petri dish and the external environment through the vent holes on the sealed cap and the circular filter membrane, thereby effectively isolating bacteria and environmental pollutants from entering the petri dish.
[0014] 3. This solution uses the threaded engagement of the sleeve and the annular groove to fix the pressure ring, and the pressure ring presses the circular filter membrane tightly against the surface of the sealing cap. Several concentrically arranged sealing rings on the pressure ring provide multi-layer sealing for the contact surface between the pressure ring and the circular filter membrane, thereby ensuring the airtightness of the contact surface. At the same time, the pressure ring is easy to install and remove, thus enabling convenient replacement of the circular filter membrane for the reuse of the culture dish. Attached Figure Description
[0015] Figure 1 A schematic diagram of the structure of a cell culture dish to prevent contamination.
[0016] Figure 2 Exploded cross-section view of the cell culture dish to prevent contamination.
[0017] Among them, 1. dish body, 2. sealing cap, 3. first external thread, 4. first internal thread, 5. vent hole, 6. circular filter membrane, 7. pressure ring, 8. sleeve, 9. second internal thread, 10. annular groove, 11. second external thread, 12. sealing ring, 13. vertical stripe, 14. annular sealing strip, 15. annular groove. Detailed Implementation
[0018] The specific embodiments of this utility model are described below to enable those skilled in the art to understand this utility model. However, it should be understood that this utility model is not limited to the scope of the specific embodiments. For those skilled in the art, as long as various changes are within the spirit and scope of this utility model as defined and determined by the appended claims, these changes are obvious. All utility model creations utilizing the concept of this utility model are within the scope of protection.
[0019] like Figure 1 and Figure 2 As shown, the anti-contamination cell culture dish of this solution includes a dish body 1 and a sealing cap 2. Both the dish body 1 and the sealing cap 2 are cylindrical with one open end. The outer side wall of the open end of the dish body 1 is provided with a first external thread 3, and the inner side wall of the open end of the sealing cap 2 is provided with a first internal thread 4 that mates with the first external thread 3. An annular sealing strip 14 is provided on the inner edge of the non-open end of the sealing cap 2. An annular groove 15 for abutting the open end of the dish body 1 is provided on the end face of the annular sealing strip 14. The cross section of the annular groove 15 is an isosceles trapezoidal shape.
[0020] This solution forms the first seal inside the dish body 1 through the threaded engagement of the dish body 1 and the sealing cap 2. At the same time, the open end of the dish body 1 extends into the annular groove 15 on the annular sealing strip 14, and the annular 7-shaped sealing strip is squeezed under the force of the threaded engagement to form the second seal inside the dish body 1. This achieves a double seal inside the dish body 1, reducing the risk of culture medium evaporation and microbial contamination.
[0021] A vent hole 5 is provided in the middle of the non-open end of the sealing cap 2. A circular filter membrane 6 is provided on the sealing cap 2 to cover the vent hole 5. The circular filter membrane 6 is detachably fixed to the sealing cap 2 by a sealing connector. The vent hole 5 and the circular filter membrane 6 on the sealing cap 2 enable CO2 / O2 exchange between the inside of the petri dish 1 and the external environment, thereby effectively isolating bacteria and environmental pollutants from entering the petri dish.
[0022] As an optional implementation, the circular filter membrane 6 is a hydrophobic polytetrafluoroethylene filter membrane with a thickness of 0.22 μm; the sealing connector includes a pressure ring 7, the outer edge of the pressure ring 7 is connected to one end of the sleeve 8, the inner side wall of the sleeve 8 is provided with a second internal thread 9, the sealing cover 2 is provided with an annular groove 10, the inner side wall of the annular groove 10 is provided with a second external thread 11 that mates with the second internal thread 9, and the pressure ring 7 presses the circular filter membrane 6 tightly against the surface of the sealing cover 2.
[0023] Specifically, the annular groove 10 and the vent hole 5 are concentrically arranged. The radial dimension of the circular filter membrane 6 is larger than the radial dimension of the vent hole 5 and smaller than the radial dimension of the outer circle of the pressure ring 7. The radial dimension of the inner circle of the pressure ring 7 is not smaller than the radial dimension of the vent hole 5. Several sealing rings 12 of different sizes are concentrically arranged on the annular end face of the pressure ring 7 that contacts the circular filter membrane 6. Several vertical stripes 13 are arranged on the outer wall of the pressure ring 7 to facilitate the twisting of the pressure ring 7.
[0024] This design uses the threaded engagement of the sleeve 8 and the annular groove 10 to fix the pressure ring 7, and the pressure ring 7 presses the circular filter membrane 6 tightly against the surface of the sealing cover 2. Several concentrically arranged sealing rings 12 on the pressure ring 7 can provide multi-layer sealing for the contact surface between the pressure ring 7 and the circular filter membrane 6, thereby ensuring the airtightness of the contact surface. At the same time, the pressure ring 7 is easy to install and remove, thereby enabling convenient replacement of the circular filter membrane 6 for the reuse of the culture dish.
Claims
1. A contamination-resistant cell culture dish, characterized in that, The device includes a dish body and a sealing cap, both of which are cylindrical with one open end. The outer side wall of the open end of the dish body is provided with a first external thread, and the inner side wall of the open end of the sealing cap is provided with a first internal thread that mates with the first external thread. A vent hole is provided in the middle of the non-open end of the sealing cap, and a circular filter membrane covering the vent hole is provided on the sealing cap. The circular filter membrane is detachably fixed to the sealing cap by a sealing connector.
2. The anti-contamination cell culture dish according to claim 1, characterized in that, The sealing connector includes a pressure ring, the outer edge of which is connected to one end of a sleeve. The inner wall of the sleeve is provided with a second internal thread. The sealing cover is provided with an annular groove. The inner wall of the annular groove is provided with a second external thread that mates with the second internal thread. The pressure ring presses the circular filter membrane tightly against the surface of the sealing cover.
3. The anti-contamination cell culture dish according to claim 2, characterized in that, The annular groove and the vent hole are concentrically arranged. The radial dimension of the circular filter membrane is greater than the radial dimension of the vent hole and less than the radial dimension of the outer circle of the pressure ring. The radial dimension of the inner circle of the pressure ring is not less than the radial dimension of the vent hole.
4. The anti-contamination cell culture dish according to claim 2, characterized in that, Several sealing rings of different sizes are concentrically arranged on the annular end face of the pressure ring that contacts the circular filter membrane.
5. The anti-contamination cell culture dish according to claim 2, characterized in that, The outer wall of the pressure ring is provided with several vertical stripes.
6. The anti-contamination cell culture dish according to claim 1, characterized in that, An annular sealing strip is provided on the inner edge of the non-open end of the sealing cap. An annular groove for abutting the open end of the vessel is provided on the end face of the annular sealing strip. The cross-section of the annular groove is an isosceles trapezoidal shape.
7. The anti-contamination cell culture dish according to claim 1, characterized in that, The circular filter membrane is a hydrophobic polytetrafluoroethylene filter membrane with a thickness of 0.22 μm.