An intermittent infiltration plant regeneration culture device

By designing a combination of tank body, clamping plate, venting tube and sealing ring, the problem of easy damage to culture when the stage is removed is solved, the stability of the stage and the durability of the venting tube are achieved, the risk of external air pollution is reduced and the airtightness of the culture environment is ensured.

CN224571981UActive Publication Date: 2026-07-31RUBBER RES INST CHINESE ACADEMY OF TROPICAL AGRI SCI
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Utility models(China)
Current Assignee / Owner
RUBBER RES INST CHINESE ACADEMY OF TROPICAL AGRI SCI
Filing Date
2025-08-21
Publication Date
2026-07-31

AI Technical Summary

Technical Problem

The stages in existing culture equipment are prone to damaging the cultures when they are removed.

Method used

An intermittent immersion plant regeneration culture device was designed, including a tank body and a tank lid. The tank body is equipped with a retaining plate and a platform. A vent pipe is snapped into the tank lid and is equipped with an air filter. A sealing ring is provided between the tank body and the tank lid. The vent pipe and the platform are detachably connected. The sealing ring achieves a tight seal between the tank lid and the tank body. Nutrient solution enters the tank body through the inlet. The vent pipe drives the platform to be stably placed on the retaining plate. When removed, the vent pipe and the platform can be detached.

Benefits of technology

This method ensures that the culture is not damaged during the removal of the stage, improves the service life of the venting tube and the stability of the stage, reduces the risk of external air pollution, and ensures the airtightness of the culture environment.

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Abstract

This utility model discloses an intermittent immersion plant regeneration culture device, including a tank body and a tank lid. The tank body and the tank lid are snapped together. A liquid inlet is provided at the bottom of the tank body. A retaining plate is provided inside the tank body, and a stage is provided on the retaining plate. A detachable vent pipe is provided on the stage, and the vent pipe is snapped together with the tank lid. One end of the vent pipe passes through the tank lid, and an air filter is provided on the vent pipe. A sealing ring is provided between the tank body and the tank lid. This utility model solves the problem in the prior art where the removal of the stage from the culture dish easily damages the culture.
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Description

Technical Field

[0001] This utility model relates to the field of tissue culture seedling cultivation technology, and in particular to an intermittent immersion plant regeneration culture device. Background Technology

[0002] The culture platform in existing culture equipment is not easy to remove from the culture tank. To address this issue, Chinese patent application number CN2021212340067 discloses a protective inoculation device for tissue culture seedlings, which includes a detachable platform that is movably disposed inside the bottle body. The platform is composed of two or more partition components spliced ​​together.

[0003] In the aforementioned prior art, when the cultivated plants are being grown on a platform, and the experimenter is taking local samples, the platform needs to be held by hand to remove it from the petri dish. However, the plants are easily damaged during the process of removing the platform. Utility Model Content

[0004] The purpose of this invention is to solve the problem that the culture is easily damaged when the stage of the culture dish is removed in the prior art.

[0005] To achieve the above objectives, the present invention adopts the following technical solution:

[0006] An intermittent immersion plant regeneration cultivation device includes a tank body and a tank lid. The tank body and the tank lid are snapped together. The bottom of the tank body is provided with a liquid inlet. A retaining plate is provided inside the tank body. A platform is provided on the retaining plate. A detachable vent pipe is provided on the platform. The vent pipe is snapped together with the tank lid. One end of the vent pipe passes through the tank lid. An air filter is provided on the vent pipe. A sealing ring is provided between the tank body and the tank lid.

[0007] Preferably, a sealing element is provided between the vent pipe and the can lid, the sealing element is snapped into the can lid, and the vent pipe is connected to the sealing element.

[0008] Preferably, the can body is provided with a plurality of limiting slots, and the bottom of the can lid is provided with a plurality of limiting shafts, the number of limiting shafts being the same as the number of limiting slots, and the limiting shafts being slidably connected to the limiting slots.

[0009] Preferably, a support block is provided at the bottom edge of the platform.

[0010] Preferably, a funnel is provided between the tank body and the liquid inlet, and the funnel connects the bottom of the tank body to the liquid inlet.

[0011] Preferably, the vent pipe is provided with a locking block, the locking block is threadedly connected to the vent pipe, the locking block is provided with no less than three elastic clamps, each elastic clamp is provided with a limiting groove, and a rubber ring is fitted on the limiting groove. The locking block is used to connect the vent pipe to the air filter device.

[0012] Preferably, the air filtration device is a butterfly air filter.

[0013] The beneficial effects proposed by this utility model are as follows:

[0014] The experimenter places the culture material on the stage, then connects the venting tube to the stage and engages it with the lid. Next, the experimenter holds the lid and places the stage into the container, simultaneously engaging the lid and body. This process uses a sealing ring to fill the gap between the container and the lid, securing them tightly and sealing the container. At this point, the stage, propelled by the venting tube, rests on the retaining plate. The retaining plate supports the stage, reducing the load on the venting tube and extending its lifespan. The inlet is used to introduce nutrient solution, allowing it to enter the tank and immerse the plants. Because the lid is secured to the tank body and connected to the stage via the vent pipe, the stage will not detach from the clamp due to buoyancy when the nutrient solution level exceeds the stage, improving its stability. This allows the plants to be placed on the stage and sealed inside the tank for experimentation. Conversely, when local sampling of plants after a period of cultivation is required, a lifting force is applied to the lid to separate it from the tank body. The experimenter then simply holds the lid and removes the stage from the tank body through the vent pipe. If the stage needs to be removed from the vent pipe, the detachable connection between the vent pipe and the stage allows separation without damaging the culture placed on it. Attached Figure Description

[0015] Figure 1 This is a perspective view of an intermittent immersion plant regeneration culture device proposed in this utility model;

[0016] Figure 2 This is a cross-sectional view of an intermittent immersion plant regeneration culture device proposed in this utility model;

[0017] Figure 3This is a partial enlarged view A in the perspective view of the intermittent immersion plant regeneration culture device proposed in this utility model;

[0018] Figure 4 This is a perspective view of the platform for an intermittent immersion plant regeneration culture device proposed in this utility model;

[0019] Figure 5 This is a perspective view of the locking block of an intermittent immersion plant regeneration culture device proposed in this utility model.

[0020] In the diagram: 1. Tank body; 2. Tank lid; 3. Liquid inlet; 4. Clamping plate; 5. Platform; 6. Vent pipe; 7. Air filter; 8. Sealing ring; 9. Seal; 10. Limiting slot; 11. Limiting shaft; 12. Support block; 13. Funnel; 14. Locking block; 15. Elastic clamp; 16. Limiting groove; 17. Rubber ring. Detailed Implementation

[0021] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments of the present utility model. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments.

[0022] Reference Figures 1 to 5An intermittent immersion plant regeneration culture device includes a tank body 1 and a tank lid 2. The tank body 1 and the tank lid 2 are snapped together. The bottom of the tank body 1 is provided with a liquid inlet 3. The tank body 1 is provided with a retaining plate 4. The retaining plate 4 is provided with a platform 5. The platform 5 is provided with a detachable vent pipe 6. The vent pipe 6 is snapped together with the tank lid 2. One end of the vent pipe 6 passes through the tank lid 2. The vent pipe 6 is provided with an air filter device 7. A sealing ring 8 is provided between the tank body 1 and the tank lid 2. Specifically, the experimenter places the culture material on the stage 5, then connects the vent pipe 6 to the stage 5 and engages the vent pipe 6 with the lid 2. Next, the experimenter holds the lid 2 and places the stage 5 into the tank body 1, simultaneously engaging the lid 2 with the tank body 1. This process uses the sealing ring 8 to fill the gap between the tank body 1 and the lid 2, ensuring a tight seal and locking of the tank body 1. At this point, the stage 5, driven by the vent pipe 6, rests on the clamping plate 4. The clamping plate 4 supports the stage 5, reducing the burden on the stage 5 from the vent pipe 6 and extending its service life. The inlet 3 is used to introduce nutrient solution, allowing it to enter the tank body 1 and immerse the plants for cultivation. Since the lid 2 is secured to the tank body 1 and connected to the platform 5 via the vent pipe 6, when the nutrient solution level exceeds the platform 5, the platform 5 will not detach from the retaining plate 4 due to buoyancy, thus improving its stability. This allows the plants for cultivation to be placed on the platform 5 and sealed inside the tank body 1 for experimental use. Conversely, when local sampling of plants that have been cultivated for a period of time is required, a lifting force is applied to the lid 2 to separate it from the tank body 1. In this case, the experimenter simply needs to hold the lid 2 and remove the platform 5 from the tank body 1 through the vent pipe 6. If the stage 5 needs to be removed from the aeration tube 6, the stage 5 can be separated from the aeration tube 6 because the aeration tube 6 and the stage 5 are detachably connected, without damaging the culture placed on the stage 5.

[0023] Specifically, a sealing element 9 is provided between the vent pipe 6 and the can lid 2. The sealing element 9 is snapped into the can lid 2, and the vent pipe 6 is connected to the sealing element 9. Since the culture needs to be sealed during the culture experiment inside the can body 1 to reduce the intrusion of external air into the can body 1 and contamination of the culture environment, the vent pipe 6, when passing through the can lid 2, needs to be connected to the can lid 2 via the sealing element 9. The sealing element 9 reduces the intrusion of external air into the can body 1 through the gaps between the components, ensuring that the air inside the can body 1 only circulates through the vent pipe 6. Simultaneously, the air entering the can body 1 is treated by the air filter device 7, thus minimizing the risk of contaminating the internal environment of the can body 1.

[0024] Specifically, the can body 1 is provided with a plurality of limiting slots 10, and the bottom of the can lid 2 is provided with a plurality of limiting shafts 11. The number of limiting shafts 11 is the same as the number of limiting slots 10, and the limiting shafts 11 are slidably connected to the limiting slots 10. When the can lid 2 is connected to the can body 1, the limiting shafts 11 are inserted into the limiting slots 10, and the plurality of limiting shafts 11 and the plurality of limiting slots 10 are simultaneously locked, so that the can lid 2 will not rotate relative to the can body 1, thus achieving a sealing effect inside the can body 1 without gaps being generated when the can lid 2 rotates relative to the can body 1.

[0025] Furthermore, when the can lid 2 is separated from the vent pipe 6, the limiting shaft 11 can serve as a support for the can lid 2, reducing the risk of the can lid 2 being placed on a contaminated surface and contaminating the main body of the can lid 2.

[0026] Specifically, a support block 12 is provided at the bottom edge of the platform 5. The support block 12 is used for placing the platform 5. When the platform 5 is taken out of the tank 1 and needs to be placed on the table surface of the experimental table, the support block 12 separates the platform 5 from the table surface, so that there is a gap between the platform 5 and the table surface. Through the gap, when the platform 5 is placed on the table surface, the roots of the cultured plants on the platform 5 can be placed in the gap, reducing the risk of root damage when the platform 5 comes into contact with the table surface.

[0027] Specifically, a funnel 13 is provided between the tank body 1 and the liquid inlet 3, and the funnel 13 connects the bottom of the tank body 1 to the liquid inlet 3. The funnel 13 is used for storing nutrient solution. When the nutrient solution is injected into the tank body 1 through the liquid inlet 3, the nutrient solution is stored in the funnel 13. When it is necessary to drain the nutrient solution, the funnel 13 helps to empty the nutrient solution from the tank body 1.

[0028] Specifically, the vent pipe 6 is provided with a locking block 14, which is threadedly connected to the vent pipe 6. The locking block 14 is provided with no fewer than three elastic clamps 15, each of which is provided with a limiting groove 16. A rubber ring 17 is fitted onto the limiting groove 16. The locking block 14 is used to connect the vent pipe 6 and the air filter device 7. When the connecting end of the air filter device 7 is connected to the vent pipe 6, the connecting end of the air filter device 7 first passes through and is inserted into the locking block 14. Then, the elastic clamps 15 are engaged with the connecting end of the air filter device 7. Finally, the rubber ring 17 is fitted into the limiting groove 16, the elastic clamps 15 are retracted, and the connecting end of the air filter device 7 is fixed in the locking block 14. In this process, in order to improve the sealing of the connection between the vent pipe 6 and the air filter device 7, a sealing strip (not shown in the figure) can be provided in the locking block 14. The sealing strip (not shown in the figure) fills the gap generated by the connection between the vent pipe 6, the air filter device 7 and the locking block 14, thereby reducing the pollution of the culture environment inside the tank 1 by external unpurified air.

[0029] Specifically, the air filtration device 7 is a butterfly air filter. Using a butterfly air filter as the air filtration device 7 offers advantages such as lower operating costs, high and uniform filtration accuracy, and strong resistance to contamination and corrosion.

[0030] The above description is only a preferred embodiment of the present utility model, but the protection scope of the present utility model is not limited thereto. Any equivalent substitutions or changes made by those skilled in the art within the technical scope disclosed in the present utility model, based on the technical solution and the inventive concept of the present utility model, should be included within the protection scope of the present utility model.

Claims

1. An intermittent immersion plant regeneration culture device comprising a tank body and a tank cover, characterized by: The can body is snapped into the can lid. The bottom of the can body is provided with a liquid inlet. The can body is provided with a retaining plate. The retaining plate is provided with a platform. The platform is provided with a detachable vent pipe. The vent pipe is snapped into the can lid. One end of the vent pipe passes through the can lid. The vent pipe is provided with an air filter. A sealing ring is provided between the can body and the can lid.

2. The device for regenerating a plant by intermittent infiltration according to claim 1, wherein: A sealing element is provided between the vent pipe and the can lid, the sealing element is snapped into the can lid, and the vent pipe is connected to the sealing element.

3. The device for regenerating a plant by intermittent infiltration according to claim 2, wherein: The can body is provided with a number of limiting slots, and the bottom of the can lid is provided with a number of limiting shafts. The number of limiting shafts is the same as the number of limiting slots, and the limiting shafts are slidably connected to the limiting slots.

4. The device for regenerating a plant by intermittent infiltration according to claim 3, wherein: A support block is provided at the bottom edge of the platform.

5. The device for regenerating plant by intermittent infiltration according to claim 4, wherein: A funnel is provided between the tank body and the liquid inlet, and the funnel connects the bottom of the tank body to the liquid inlet.

6. The device for regenerating plant by intermittent infiltration according to claim 5, wherein: The vent pipe is provided with a locking block, which is threadedly connected to the vent pipe. The locking block is provided with no less than three elastic clamps, each of which is provided with a limiting groove. A rubber ring is fitted on the limiting groove. The locking block is used to connect the vent pipe to the air filter device.

7. The device for regenerating plant by intermittent infiltration according to claim 6, wherein: The air filtration device is a butterfly air filter.