High-precision micropipette tip

Abstract

This utility model discloses a high-precision micropipette tip, relating to the field of laboratory liquid transfer instruments. It includes an integrally formed connecting part, transition chamber, and a suction part. The inner wall of the connecting part has multiple intermittently distributed semi-circular annular sealing ridges, and the middle of the outer wall has symmetrical rectangular anti-slip protrusions with transverse stripes. The transition chamber is a conical cavity with a spiral guide groove on its inner wall. The suction part is a slender tubular structure with an inner diameter that decreases linearly from top to bottom. The bottom end has a rounded 45° bevel, and the outer wall has volume graduations and Arabic numeral volume values. The inner walls of the connecting part, transition chamber, and suction part are all mirror-polished and coated with a hydrophobic coating. In use, through the synergistic effect of multiple seals, spiral guide, linear diameter change, hydrophobic coating, and precise graduations, it effectively solves problems such as insufficient sealing, excessive liquid residue, easy damage to containers, and easy wear of graduations in existing pipette tips, thus improving the accuracy, stability, and ease of operation of micropipettes.

Description

Technical Field

[0001] This utility model relates to the field of laboratory liquid transfer instruments, specifically a high-precision micro-pipette tip. Background Technology

[0002] In molecular biology, pathology, and clinical laboratory fields, micropipettes are the core tool for precise liquid transfer. The pipette tip, as the part that directly contacts the liquid, directly determines the pipetting accuracy and experimental reliability. Existing micropipette tips have several technical defects: the connecting part often uses a single-ring embossed or smooth inner wall design, making its sealing performance susceptible to installation errors or wear from repeated use, leading to air leakage during pipetting. This leakage can cause significant volume errors, especially in 1-10 μL micropipettes; the transition chamber is often a smooth conical structure, which easily creates turbulence during high-speed pipetting and causes severe residue buildup due to surface tension, further amplifying measurement errors; the aspiration section often has a constant or stepped diameter design with a sharp cutting edge at the bottom, easily puncturing centrifuge tubes, culture dishes, and other containers, and causing liquid splashing during aspiration, affecting pipetting accuracy; the outer wall graduations are often printed or molded, which are prone to blurring due to friction and wear, and the graduation intervals are relatively large (usually ≥2 μL), failing to meet the visual measurement requirements of micropipettes. These issues make it difficult for existing pipette tips to achieve an error standard within ±2% in scenarios with stringent precision requirements, such as PCR reaction system configuration and cell suspension inoculation, severely restricting the repeatability and reliability of experimental results.

[0003] Therefore, those skilled in the art have provided a high-precision micropipette tip to solve the problems mentioned in the background art. Utility Model Content

[0004] The purpose of this invention is to provide a high-precision micropipette tip to solve the problems mentioned in the background art.

[0005] To achieve the above objectives, this utility model provides the following technical solution:

[0006] A high-precision micro-pipette tip includes an integrally formed connecting part, a transition cavity, and a suction part, all made of polypropylene. The connecting part is a cylindrical sleeve with an open top, and its inner wall has multiple intermittently distributed annular sealing ridges with a semi-circular cross-section. The transition cavity is a conical cavity with its upper end smoothly connected to the lower end of the connecting part. The inner wall of the transition cavity has a spiral guide groove. The suction part is a slender tubular structure with its upper end coaxially connected to the lower end of the transition cavity. The inner diameter of the suction part decreases linearly from top to bottom, and the bottom end has a 45° beveled surface with a rounded cutting edge. The outer wall of the suction part has a volume scale.

[0007] As a further embodiment of this utility model: the inner wall of the connecting part is clearance-fitted with the pipette body, and the annular sealing ridge is made of elastic polypropylene material, which has the characteristic of being deformable to compensate for installation errors.

[0008] As a further embodiment of this utility model: the outer wall of the connecting part is provided with symmetrical rectangular anti-slip protrusions in the middle, and the surface of the rectangular anti-slip protrusions is provided with horizontal stripes, which are distributed at intervals along the length direction of the rectangular anti-slip protrusions.

[0009] As a further embodiment of this utility model: the taper of the transition cavity is adapted to the pitch of the spiral guide groove, the starting end of the spiral guide groove is located in the upper region of the transition cavity, and the end extends to the connection between the transition cavity and the liquid absorption part.

[0010] As a further improvement of this utility model: the volume scale lines are made by laser engraving process, and Arabic numeral volume values ​​are marked next to the scale lines. The intervals of the volume scale lines correspond to the commonly used measurement units for micro-pipettes.

[0011] As a further improvement of this utility model: the inner walls of the connecting part, the transition cavity and the liquid absorption part are all mirror polished and coated with a hydrophobic coating.

[0012] Compared with existing technologies, the beneficial effects of this invention are as follows: The multi-ring semi-circular annular sealing ridges on the inner wall of the connecting part are made of elastic polypropylene. Through a combination of multiple sealing designs and gap fits, it can effectively compensate for installation deviations, avoid air leakage during pipetting, and ensure high volumetric accuracy even in micro-pipettes of 1-10μL. It also ensures ease of installation and disassembly, solving the problem of decreased accuracy caused by poor sealing performance in existing pipette tips. The spiral guide groove on the inner wall of the transition chamber is adapted to the taper of the conical cavity, guiding the liquid to flow smoothly along the spiral path and effectively suppressing turbulence during high-speed pipetting. Simultaneously, the mirror polishing treatment and hydrophobic coating on the inner wall significantly reduce liquid residue on the wall, greatly reducing metering deviations, especially in micro-pipette scenarios. The design delivers outstanding results. The linearly decreasing inner diameter of the aspiration section from top to bottom creates a stable flow gradient for the liquid. The smooth, rounded edge of the 45° bevel at the bottom avoids damage to centrifuge tubes, culture dishes, and other containers, while increasing the liquid contact area, reducing splashing, and improving the accuracy and safety of pipetting. The rectangular anti-slip protrusions and transverse stripes on the outer wall of the connecting section increase friction during operation, facilitating stable installation and removal of the pipette tip, especially suitable for gloved operation. The volume scale lines and Arabic numeral volume values ​​on the outer wall of the aspiration section, produced using laser engraving, are not only durable but also correspond to commonly used units of measurement for micropipettes, enabling visualized measurement of the pipetting process and meeting the needs of high-precision experiments for measurement visualization. Overall, through the synergistic effect of its various structures, this invention effectively adapts to the stringent requirements of micropipettes in fields such as molecular biology and pathology, such as PCR reaction system preparation and cell suspension inoculation, significantly improving the reproducibility and reliability of experimental results. Attached Figure Description

[0013] Figure 1 This is a schematic diagram of the overall structure of a high-precision micropipette tip.

[0014] Figure 2 This is a front cross-sectional view of a high-precision micropipette tip.

[0015] Figure 3 This is a schematic diagram of the anti-slip protrusion in a high-precision micropipette tip.

[0016] Figure 4 This is a schematic diagram of the liquid aspiration port in a high-precision micropipette tip.

[0017] In the diagram: 1. Connecting part; 2. Transition cavity; 3. Liquid suction part; 4. Annular sealing ridge; 5. Anti-slip protrusion; 6. Spiral guide groove; 7. Beveled surface; 8. Capacity scale line; 9. Horizontal stripe. Detailed Implementation

[0018] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the protection scope of the present utility model.

[0019] Example 1

[0020] Reference Figure 1-4 The figure shows a high-precision micropipette tip, comprising an integrally formed connecting part 1, a transition cavity 2, and a suction part 3, all made of polypropylene. Polypropylene is characterized by good chemical stability and strong corrosion resistance, making it suitable for contact with various experimental liquids. The connecting part 1 is a cylindrical sleeve with an open top, its shape adapted to the pipette body for easy connection and fixation. The inner wall is provided with multiple intermittently distributed annular sealing ridges 4. The annular sealing ridges 4 have a semi-circular cross-section, which enhances the fit with the pipette body, improves sealing performance, and prevents air leakage during pipetting that could affect accuracy. The transition cavity 2 is a conical cavity, with its upper end flush with the lower end of the connecting part 1. The smooth transition connection guides the smooth flow of liquid and reduces liquid stagnation. The inner wall of the transition cavity 2 is provided with a spiral guide groove 6, which can regulate the liquid flow path and avoid turbulence when the liquid is transferred at high speed, thus reducing the measurement error caused by turbulence. The liquid suction part 3 is a slender tubular structure, which is convenient to insert into the container to suck up a small amount of liquid. The upper end is coaxially connected to the lower end of the transition cavity 2 to ensure the continuity of liquid flow. The inner diameter of the liquid suction part 3 decreases linearly from top to bottom. The bottom end is a 45° beveled surface 7, which increases the contact area with the liquid and facilitates accurate liquid suction. The cutting edge of the beveled surface 7 is rounded to avoid damaging the container. The outer wall of the liquid suction part 3 is provided with a capacity scale line 8.

[0021] Furthermore, the inner wall of the connecting part 1 is in clearance fit with the pipette body, and the annular sealing ridge 4 is made of elastic polypropylene material, which has the characteristic of being deformable to compensate for installation errors. By utilizing its deformable characteristics to compensate for errors during installation, the sealing performance of the connection is further ensured and air leakage is prevented.

[0022] Example 2

[0023] Reference Figure 1-4 The figure shows that this embodiment is based on the previous embodiment, but differs from the previous embodiment in that the middle of the outer wall of the connecting part 1 is provided with symmetrical rectangular anti-slip protrusions 5, which provide a point of force for hand operation and facilitate the installation and removal of the gun head. The surface of the rectangular anti-slip protrusions 5 is provided with horizontal stripes 9, which are distributed at intervals along the length of the rectangular anti-slip protrusions 5, which can increase friction and prevent slipping during operation, especially suitable for use when wearing gloves.

[0024] Furthermore, the taper of the transition cavity 2 is adapted to the pitch of the spiral guide groove 6, so that the liquid flows smoothly along the spiral path in the transition cavity 2. The starting end of the spiral guide groove 6 is located in the upper region of the transition cavity 2, and the end extends to the connection between the transition cavity 2 and the liquid absorption part 3, which can guide the liquid flow throughout the process and reduce the liquid residue on the wall in the transition area.

[0025] Furthermore, the volume scale line 8 is made using laser engraving technology, which is more wear-resistant and less prone to blurring compared to traditional printing or molding scales. Arabic numeral volume values ​​are marked next to the scale line, and the intervals of the volume scale line 8 correspond to commonly used measurement units for micro-pipettes, which can intuitively display the volume of liquid transferred, realize visual measurement, and improve the accuracy of pipetting.

[0026] Furthermore, the inner walls of the connecting part 1, the transition cavity 2, and the liquid absorption part 3 are all mirror-polished, which can reduce the adsorption of liquid on the wall surface. In addition, the inner walls are coated with a hydrophobic coating, which further reduces the phenomenon of liquid sticking to the wall, significantly reduces liquid residue, and ensures the accuracy of liquid transfer.

[0027] This invention's high-precision micro-volume pipette tip achieves accurate pipetting through the synergistic effect of multiple structures: During use, the connecting part 1 is fitted into the pipette body. Multiple semi-circular annular sealing ridges 4 on the inner wall, due to the deformable properties of the elastic polypropylene material, form a multi-layered seal with the pipette body. This ensures easy installation through a gap fit and compensates for installation errors, preventing air leakage. The rectangular anti-slip protrusions 5 and horizontal stripes 9 on the outer wall of the connecting part 1 increase friction, facilitating stable installation and disassembly. During pipetting, liquid is drawn in through the 45° beveled surface 7 at the bottom of the suction part 3. The rounded, smooth edge of this beveled surface 7 prevents damage to the container and increases the liquid contact area to reduce splashing. The linearly decreasing inner diameter of the suction part 3 creates a stable flow rate gradient along the axial direction. Combined with the mirror polishing and hydrophobic coating on the inner wall, this significantly reduces residue buildup on the container walls. After the liquid enters the transition chamber 2, the spiral guide groove 6 on the inner wall matches the taper of the conical cavity, guiding the liquid to flow smoothly along the spiral path, suppressing turbulence, and ensuring that the liquid enters the connecting part 1 without disturbance. During drainage, the spiral guide groove 6 guides the liquid out quickly in the opposite direction, further reducing residue. During the suction process, the volume scale 8 and Arabic numeral volume values ​​laser-engraved on the outer wall can be observed in real time to achieve visualized measurement. Through multiple sealing with sealing ridges, turbulence suppression by the spiral guide groove 6, flow rate stabilization by linear diameter change, residue control by the hydrophobic coating, and visualization assistance by precise scale, the various structural parameters are matched and coordinated, ultimately achieving a dispensing error within ±0.5% within the 1-100μL range.

[0028] It will be apparent to those skilled in the art that this invention is not limited to the details of the exemplary embodiments described above, and that it can be implemented in other specific forms without departing from the spirit or essential characteristics of this invention. Therefore, the embodiments should be considered illustrative and non-limiting in all respects, and the scope of this invention is defined by the appended claims rather than the foregoing description. Thus, it is intended that all variations falling within the meaning and scope of equivalents of the claims be included within this invention. No reference numerals in the claims should be construed as limiting the scope of the claims.

[0029] Furthermore, it should be understood that although this specification describes embodiments, not every embodiment contains only one independent technical solution. This narrative style is merely for clarity. Those skilled in the art should consider the specification as a whole, and the technical solutions in each embodiment can also be appropriately combined to form other embodiments that can be understood by those skilled in the art.

Claims

1. A high precision micropipette tip, characterized in that, The device includes an integrally formed connecting part (1), a transition cavity (2), and a liquid absorption part (3), all of which are made of polypropylene. The connecting part (1) is a cylindrical sleeve with an open top and multiple annular sealing ridges (4) distributed at intervals on the inner wall. The annular sealing ridges (4) have a semi-circular cross section. The transition cavity (2) is a conical cavity with a smooth transition connection between the upper end and the lower end of the connecting part (1). The inner wall of the transition cavity (2) is provided with a spiral guide groove (6). The liquid absorption part (3) is a slender tubular structure with its upper end coaxially connected to the lower end of the transition cavity (2). The inner diameter of the liquid absorption part (3) decreases linearly from top to bottom. The bottom end is a 45° beveled surface (7), and the cutting edge of the beveled surface (7) is rounded. The outer wall of the liquid absorption part (3) is provided with a capacity scale line (8).

2. A high precision micropipette tip as claimed in claim 1, wherein, The inner wall of the connecting part (1) is in clearance fit with the pipette body, and the annular sealing ridge (4) is made of elastic polypropylene material, which has the characteristic of being deformable to compensate for installation errors.

3. The high precision micropipette tip of claim 1, wherein, The connecting part (1) has symmetrical rectangular anti-slip protrusions (5) in the middle of its outer wall. The surface of the rectangular anti-slip protrusions (5) is provided with horizontal stripes (9), which are distributed at intervals along the length of the rectangular anti-slip protrusions (5).

4. The high precision micropipette tip of claim 1, wherein, The taper of the transition cavity (2) is adapted to the pitch of the spiral guide groove (6). The starting end of the spiral guide groove (6) is located in the upper region of the transition cavity (2), and the end extends to the connection between the transition cavity (2) and the liquid absorption part (3).

5. The high precision micropipette tip of claim 1, wherein, The volume scale lines (8) are made by laser engraving. Arabic numeral volume values ​​are marked next to the scale lines. The intervals of the volume scale lines (8) correspond to the commonly used measurement units for micro-pipettes.

6. The high precision micropipette tip of claim 1, wherein, The inner walls of the connecting part (1), the transition cavity (2) and the liquid absorption part (3) are all mirror polished and coated with a hydrophobic coating.

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