Cross-contamination prevention micropipette assembly for ultrasensitive single-molecule detection kits

Abstract

This utility model relates to the field of reagent kit technology, specifically to a cross-contamination-preventing micropipette assembly for an ultrasensitive single-molecule detection reagent kit. The assembly includes a reagent kit body, with a detection groove and a locking rod respectively disposed on the top of the body. A sliding groove is formed on the front surface of the body, and a sliding block is disposed inside the groove. An electric actuator is fixedly mounted on the top surface of the sliding block, and a mounting plate is fixedly disposed on the telescopic end of the electric actuator. A pipette pump and a hollow cylinder are respectively disposed on the top of the mounting plate, and a pipette tip is disposed at the bottom of the hollow cylinder. A connecting mechanism is provided between the pipette tip and the hollow cylinder. Through the design of this cross-contamination-preventing micropipette assembly for the ultrasensitive single-molecule detection reagent kit, the structural design of the connecting mechanism facilitates convenient assembly and disassembly of the pipette tip, allowing for easy replacement of the pipette tip according to different samples, effectively preventing cross-contamination.

Description

Technical Field

[0001] This utility model relates to the field of reagent kit technology, specifically to a cross-contamination-preventing micropipette component for an ultrasensitive single-molecule detection reagent kit. Background Technology

[0002] In the process of detecting ultrasensitive single molecules, pipetting operations are required using a pipetting assembly.

[0003] Currently used ultrasensitive single-molecule detection kits rely on pipette tips to absorb samples, as these tips are not easily disassembled. This can easily lead to cross-contamination of samples and is detrimental to subsequent use.

[0004] In summary, this invention addresses the problems in the background art by designing a cross-contamination-resistant micropipette assembly for an ultrasensitive single-molecule detection kit. Utility Model Content

[0005] The purpose of this invention is to provide a cross-contamination-resistant micropipette assembly for an ultrasensitive single-molecule detection kit, in order to solve the problems mentioned in the background art.

[0006] To achieve the above objectives, this utility model provides the following technical solution:

[0007] A cross-contamination-resistant micropipette assembly for an ultrasensitive single-molecule detection kit includes a kit body. The top of the kit body has a detection groove and a locking rod. A locking spring is fitted around the outside of the locking rod. A groove is formed on the front surface of the kit body, and a sliding block is disposed inside the groove. An electric actuator is fixedly mounted on the top surface of the sliding block. A mounting plate is fixedly mounted on the telescopic end of the electric actuator. A pipette pump and a hollow cylinder are respectively disposed on the top of the mounting plate. A pipette tip is disposed at the bottom of the hollow cylinder, and a connecting mechanism is provided between the pipette tip and the hollow cylinder.

[0008] The connecting mechanism includes an upper mounting block, a lower mounting block, a contact ring, and a groove. The upper mounting block is fixedly installed on the outer bottom of the hollow cylinder. A positioning post is fixedly installed at the bottom of the upper mounting block. A friction block is eccentrically rotatably installed at the bottom end of the positioning post. The groove is opened on the bottom surface of the hollow cylinder. A support spring and a compensation pad are respectively installed inside the groove. The contact ring is fixedly installed on the top surface of the pipette tip. The lower mounting block is fixedly installed on the outer surface of the pipette tip. A positioning hole is opened on the top surface of the lower mounting block.

[0009] As a preferred embodiment of this utility model, the outer surface of the sliding block is slidably fitted to the inner wall of the sliding groove.

[0010] As a preferred embodiment of this utility model, the bottom end of the locking rod movably penetrates through the top surface of the reagent kit body and extends into the slide groove, and the two ends of the locking spring are respectively in fixed contact with the top surface of the reagent kit body and the end of the locking rod.

[0011] As a preferred embodiment of this utility model, the input end of the pipette pump is connected to the inner wall of the top of the hollow cylinder through a conduit, the bottom of the pipette tip is conical, and scale lines are provided on the outer side of the pipette tip.

[0012] As a preferred embodiment of this utility model, the outer surface of the positioning post is in contact with the positioning hole, and the radial dimension of the friction block is consistent with the diameter of the positioning post.

[0013] As a preferred embodiment of this utility model, the top surface of the friction block is provided with anti-slip patterns, and the friction block forms frictional contact with the bottom surface of the lower mounting block through the anti-slip patterns.

[0014] As a preferred embodiment of this utility model, the cross-sectional shape of the groove is annular, and the inner wall of the groove is in contact with the outer surface of the contact ring. The compensation gasket is an annular silicone sealant. The support springs are distributed annularly at equal intervals about the bottom surface of the groove, and the two ends of the support springs are fixed to the inner wall of the groove and the top surface of the compensation gasket, respectively.

[0015] Compared with the prior art, the beneficial effects of this utility model are:

[0016] In this invention, the anti-cross-contamination micropipette assembly of the ultrasensitive single-molecule detection kit utilizes the structural design of the connecting mechanism to achieve rapid positioning between the pipette tip and the hollow cylinder when the positioning column contacts the positioning block. The eccentric rotation of the friction block causes a frictional self-locking between the friction block and the lower mounting block, thus fixing the pipette tip to the hollow cylinder. Simultaneously, the elastic support of the compensating gasket by the supporting spring ensures a sealed contact between the compensating gasket and the contact ring, thereby facilitating convenient disassembly and assembly of the pipette tip. This allows for easy replacement of the pipette tip according to different samples, effectively preventing cross-contamination and benefiting subsequent use. Attached Figure Description

[0017] Figure 1 This is a schematic diagram of the overall structure of this utility model;

[0018] Figure 2 For the present utility model Figure 1 Enlarged structural diagram of point A in the middle;

[0019] Figure 3 This is a schematic diagram of the bottom structure of the hollow cylinder of this utility model;

[0020] Figure 4 This is a schematic diagram of the structure of the pipette tip of this utility model;

[0021] Figure 5 This is a schematic diagram of the bottom structure of the compensation gasket of this utility model.

[0022] In the diagram: 1. Reagent kit body; 2. Detection chamber; 3. Locking rod; 301. Locking spring; 4. Slide groove; 401. Sliding block; 402. Electric actuator; 5. Mounting plate; 501. Pipette pump; 502. Hollow cylinder; 503. Pipette tip; 6. Connecting mechanism; 601. Upper mounting block; 602. Lower mounting block; 603. Contact ring; 604. Groove; 605. Positioning post; 606. Friction block; 607. Support spring; 608. Compensation shim; 609. Positioning hole. Detailed Implementation

[0023] The technical solutions of the present utility model will be clearly and completely described below with reference to the embodiments of the present utility model. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. All other embodiments obtained by those skilled in the art based on the embodiments of the present utility model without creative effort are within the protection scope of the present utility model.

[0024] To facilitate understanding of this utility model, a more comprehensive description of the utility model will be given below with reference to the accompanying drawings, and several embodiments of the utility model will be provided. However, the utility model can be implemented in many different forms and is not limited to the embodiments described herein. On the contrary, the purpose of providing these embodiments is to make the disclosure of the utility model more thorough and complete.

[0025] It should be noted that when an element is referred to as being "fixed to" another element, it can be directly on the other element or there may be an intervening element. When an element is referred to as being "connected to" another element, it can be directly connected to the other element or there may be an intervening element. The terms "vertical," "horizontal," "left," "right," and similar expressions used in this document are for illustrative purposes only.

[0026] Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention pertains. The terminology used herein is for the purpose of describing particular embodiments only and is not intended to limit the invention. The term "and / or" as used herein includes any and all combinations of one or more of the associated listed items.

[0027] For examples, please refer to Figure 1-5This utility model provides a technical solution:

[0028] The anti-cross-contamination micropipette assembly of the ultrasensitive single-molecule detection kit includes a kit body 1. The top of the kit body 1 is provided with a detection groove 2 and a locking rod 3. A locking spring 301 is sleeved on the outside of the locking rod 3. A sliding groove 4 is opened on the front surface of the kit body 1. A sliding block 401 is provided inside the sliding groove 4. An electric push rod 402 is fixedly installed on the top surface of the sliding block 401. An installation plate 5 is fixedly provided on the telescopic end of the electric push rod 402. A pipette pump 501 and a hollow cylinder 502 are respectively provided on the top of the installation plate 5. A pipette tip 503 is provided at the bottom of the hollow cylinder 502. A connecting mechanism 6 is provided between the pipette tip 503 and the hollow cylinder 502.

[0029] Specifically, the outer surface of the sliding block 401 is slidably fitted against the inner wall of the slide groove 4, the bottom end of the locking rod 3 moves through the top surface of the reagent kit body 1 and extends into the slide groove 4, and the two ends of the locking spring 301 are fixedly in contact with the top surface of the reagent kit body 1 and the end of the locking rod 3, respectively.

[0030] In this embodiment, the slide groove 4 is mainly used to slide with the sliding block 401, which facilitates the lateral movement of the mounting plate 5, the hollow cylinder 502 and the pipette tip 503. At the same time, with the combination of the locking spring 301 and the locking rod 3, the sliding block 401 can be easily moved and limited, which meets the needs of the user.

[0031] Specifically, the input end of the pipette pump 501 is connected to the inner wall of the top of the hollow cylinder 502 through a conduit, the bottom of the pipette tip 503 is conical, and scale lines are provided on the outer side of the pipette tip 503.

[0032] In this embodiment, the electric actuator 402 is mainly used to adjust the vertical displacement of the mounting plate 5, causing the mounting plate 5 to move the pipette tip 503 closer to the inside of the detection tank 2. The pipette pump 501 is mainly used to draw suction from the inside of the hollow cylinder 502, causing the sample to enter the inside of the pipette tip 503, thereby achieving the function of pipetting the sample.

[0033] In this embodiment, please refer to Figure 1 , Figure 3 , Figure 4 and Figure 5The connecting mechanism 6 includes an upper mounting block 601, a lower mounting block 602, a contact ring 603, and a groove 604. The upper mounting block 601 is fixedly installed on the outer bottom of the hollow cylinder 502. A positioning post 605 is fixedly provided at the bottom of the upper mounting block 601. A friction block 606 is eccentrically rotatably provided at the bottom end of the positioning post 605. The groove 604 is opened on the bottom surface of the hollow cylinder 502. A support spring 607 and a compensation pad 608 are respectively provided inside the groove 604. The contact ring 603 is fixedly installed on the top surface of the pipette tip 503. The lower mounting block 602 is fixedly installed on the outer surface of the pipette tip 503. A positioning hole 609 is opened on the top surface of the lower mounting block 602.

[0034] Specifically, the outer surface of the positioning post 605 fits into the positioning hole 609, the radial dimension of the friction block 606 is consistent with the diameter of the positioning post 605, the top surface of the friction block 606 is provided with anti-slip patterns, and the friction block 606 forms frictional contact with the bottom surface of the lower mounting block 602 through the anti-slip patterns, the cross-sectional shape of the groove 604 is annular, and the inner wall of the groove 604 is fitted into contact with the outer surface of the contact ring 603, the compensation gasket 608 is an annular silicone sealant, the support spring 607 is annularly distributed at equal intervals about the bottom surface of the groove 604, and the two ends of the support spring 607 are respectively fixed to the inner wall of the groove 604 and the top surface of the compensation gasket 608;

[0035] In this embodiment, the positioning post 605 is mainly used to contact the positioning hole 609. Under the eccentric rotation of the friction block 606 and the positioning post 605, a frictional contact force is generated between the friction block 606 and the lower mounting support block 602, which realizes the quick fixation of the pipette tip 503. At the same time, the support spring 607 can contact the compensation gasket 608, which causes the compensation gasket 608 to make a sealing contact with the contact ring 603, thus meeting the installation or disassembly requirements of the pipette tip 503 and facilitating subsequent replacement and installation.

[0036] The working process of this utility model is as follows: When using the anti-cross-contamination micropipette assembly of the ultrasensitive single-molecule detection kit, the sliding block 401 and the sliding groove 4 slide together, causing the sliding block 401 to move the pipette tip 503 to the desired position. Simultaneously, the electric actuator 402 is activated, retracting and bringing the pipette tip 503 into contact with the sample in the detection chamber 2. At the same time, the pipetting pump 501 is activated, causing the sample in the detection chamber 2 to enter the pipette tip 503. Inside the 03, when different samples need to be pipetted, an external force is applied to the friction block 606, causing the friction block 606 to completely detach from the lower mounting block 602. This causes the lower mounting block 602 to disengage the positioning hole 609 from the positioning post 605. During the disengagement process, the support spring 607 gradually extends and pushes the contact ring 603 to separate from the groove 604, thereby achieving the function of convenient disassembly of the pipette tip 503. This facilitates the replacement of a new pipette tip 503, effectively prevents cross-contamination between different samples, and meets the needs of users.

[0037] Although embodiments of the present invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made to these embodiments without departing from the principles and spirit of the present invention, the scope of which is defined by the appended claims and their equivalents.

Claims

1. Anti-cross contamination micro pipetting assembly for ultrasensitive single molecule detection kits, comprising a kit body (1) characterized in that: The top of the reagent kit body (1) is provided with a detection groove (2) and a locking rod (3). A locking spring (301) is sleeved on the outside of the locking rod (3). A sliding groove (4) is opened on the front surface of the reagent kit body (1). A sliding block (401) is provided inside the sliding groove (4). An electric push rod (402) is fixedly installed on the top surface of the sliding block (401). An installation plate (5) is fixedly provided on the telescopic end of the electric push rod (402). A pipette pump (501) and a hollow cylinder (502) are respectively provided on the top of the installation plate (5). A pipette tip (503) is provided at the bottom of the hollow cylinder (502). A connecting mechanism (6) is provided between the pipette tip (503) and the hollow cylinder (502). The connecting mechanism (6) includes an upper mounting block (601), a lower mounting block (602), a contact ring (603), and a groove (604). The upper mounting block (601) is fixedly installed on the outer bottom of the hollow cylinder (502). A positioning post (605) is fixedly provided at the bottom of the upper mounting block (601). A friction block (606) is eccentrically rotatably provided at the bottom end of the positioning post (605). The groove (604) is opened on the bottom surface of the hollow cylinder (502). A support spring (607) and a compensation pad (608) are respectively provided inside the groove (604). The contact ring (603) is fixedly installed on the top surface of the pipette tip (503). The lower mounting block (602) is fixedly installed on the outer surface of the pipette tip (503). A positioning hole (609) is opened on the top surface of the lower mounting block (602).

2. The cross-contamination resistant micropipetting assembly for ultrasensitive single molecule detection kits of claim 1, wherein: The outer surface of the sliding block (401) is slidably fitted against the inner wall of the groove (4).

3. The cross-contamination resistant micropipetting assembly for ultrasensitive single molecule detection kits of claim 1, wherein: The bottom end of the locking rod (3) extends through the top surface of the reagent kit body (1) and into the slide groove (4). The two ends of the locking spring (301) are in fixed contact with the top surface of the reagent kit body (1) and the end of the locking rod (3), respectively.

4. The cross-contamination resistant micropipetting assembly for ultrasensitive single molecule detection kits of claim 1, wherein: The input end of the pipette pump (501) is connected to the inner wall of the top of the hollow cylinder (502) through a conduit. The bottom of the pipette tip (503) is conical, and scale lines are provided on the outer side of the pipette tip (503).

5. The cross-contamination resistant micropipetting assembly for ultrasensitive single molecule detection kits of claim 1, wherein: The outer surface of the positioning post (605) is in contact with the positioning hole (609), and the radial dimension of the friction block (606) is consistent with the diameter of the positioning post (605).

6. The cross-contamination resistant micropipetting assembly for ultrasensitive single molecule detection kits of claim 1, wherein: The top surface of the friction block (606) is provided with anti-slip patterns, and the friction block (606) forms frictional contact with the bottom surface of the lower mounting block (602) through the anti-slip patterns.

7. The cross-contamination resistant micropipetting assembly for ultrasensitive single molecule detection kits of claim 1, wherein: The groove (604) has a ring-shaped cross-section, and the inner wall of the groove (604) is in contact with the outer surface of the contact ring (603). The compensation gasket (608) is a ring-shaped silicone sealant. The support springs (607) are distributed in a ring at equal intervals about the bottom surface of the groove (604), and the two ends of the support springs (607) are fixed to the inner wall of the groove (604) and the top surface of the compensation gasket (608), respectively.

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