Amino acid sample prep centrifuge tubes

Abstract

This utility model discloses an amino acid sample pretreatment centrifuge tube, applied in the field of centrifuge tube technology. It includes a centrifuge tube body, a sealing cap on the top of the centrifuge tube body, a lever plate adhered to the surface of the sealing cap, a rubber pad attached to the bottom of the lever plate, a first limiting plate adhered to the bottom of the sealing cap, a second limiting plate adhered to the surface of the centrifuge tube body, a slanted plate adhered to the surface of the second limiting plate, a female hook and loop fastener adhered to one side of the rubber pad, and a female hook and loop fastener adhered to the other side of the rubber pad. The female and female hook and loop fasteners are bonded together. This utility model, through the rubber pad, effectively utilizes its softness to protect the fingers of workers, improving worker comfort and increasing the efficiency of the centrifuge tube body. The female and female hook and loop fasteners facilitate locking the rubber pad.

Description

Technical Field

[0001] This invention belongs to the field of centrifuge tube technology, and specifically relates to centrifuge tubes for amino acid sample pretreatment. Background Technology

[0002] Centrifuge tubes are tubular containers used in laboratories for sample separation, storage, and centrifugation. They are widely used in fields such as biochemistry, molecular biology, and clinical medicine. Their core function is to achieve rapid separation of different components in a mixture through the centrifugal force generated by the centrifuge, such as precipitating organelles and biomolecules. They separate substances of different densities through centrifugal force and are the basic tools for processing biological samples and chemical reagents. They are made of high-quality transparent polymer material polypropylene (PP) and have been widely used in biochemical and molecular biological research. Centrifuge tubes are used in conjunction with a centrifuge. Depending on the experimental requirements, pour the liquid sample into the centrifuge tube, being careful not to exceed the tube's markings to avoid spillage. After pouring the sample, seal the centrifuge tube with a matching cap, ensuring the cap is secure to prevent leakage during centrifugation. Set the centrifuge speed and time according to the experimental needs; higher speeds and longer centrifugation times are typically used to separate heavier components, while lower speeds and shorter times are used to separate lighter components. Place the sealed centrifuge tube into the centrifuge, ensuring the cap is closed and locked securely. Start the centrifuge and allow it to run for the required time. After centrifugation, stop the centrifuge and carefully remove the centrifuge tube, being careful not to invert or shake it to avoid mixing the separated components. Observe the sample in the centrifuge tube; heavier components will settle at the bottom, while lighter components will float on top. If necessary, transfer the two components to separate containers for further analysis and research. Currently, Chinese utility model patent CN218113616U discloses a centrifuge tube to address the problems of existing centrifuge tubes having large diameters, which easily cause sample splashing and contamination when the cap is opened, and the open cap occupying a large space, limiting the amount of sample that can be evaporated at one time during vacuum evaporation. The centrifuge tube includes a tube body and a cap. Multiple graduation lines are provided on the outer wall of the tube body. The cap is adapted to the size of the tube body's opening to seal the opening. A cap hole with internal and external communication is provided in the middle area of ​​the cap, and a detachable cap is provided to the cap hole. During vacuum evaporation experiments, the small diameter of the cap hole effectively prevents sample splashing. When handling easily volatile samples, the small-diameter cap hole effectively avoids sample loss. The cap remains closed during the experiment, saving space in the vacuum evaporation instrument and increasing the amount of sample that can be evaporated at one time.

[0003] While the aforementioned centrifuge tubes are connected to the outside environment through a small-diameter cap opening, effectively preventing sample splashing and minimizing sample loss when handling volatile samples, and the cap remains closed during experiments, saving space in the vacuum drying instrument and increasing the amount of sample that can be dried at once, the following problems exist in practical use: In biological experiments, centrifuge tubes typically need to be opened and closed frequently. Manually opening and closing centrifuge tubes repeatedly can cause redness and pain in the experimenter's fingers, and in severe cases, may even lead to skin damage and joint strain. Therefore, we propose amino acid sample pretreatment centrifuge tubes to address these issues. Utility Model Content

[0004] The purpose of this invention is to provide a centrifuge tube for amino acid sample pretreatment, which has the advantages of improving the comfort of experimental personnel and also improving the stability of the seal.

[0005] The above-mentioned technical objective of this utility model is achieved through the following technical solution: an amino acid sample pretreatment centrifuge tube, including a centrifuge tube body, a sealing cap on the top of the centrifuge tube body, a toggle plate adhered to the surface of the sealing cap, and a rubber pad attached to the bottom of the toggle plate, the rubber pad being made of food-grade silicone material resistant to chemical corrosion.

[0006] The above technical solution utilizes the rubber pads to effectively protect the fingers of staff, improving their comfort and increasing the efficiency of the centrifuge tubes.

[0007] The present invention is further configured such that a first limiting plate is bonded to the bottom of the sealing cap, the first limiting plate is made of medical-grade silicone with better elasticity, a second limiting plate is bonded to the surface of the centrifuge tube body, and an inclined plate is bonded to the surface of the second limiting plate.

[0008] The above technical solution uses a first limiting plate, an inclined plate, and a second limiting plate to limit the sealing cover and prevent it from loosening and falling off during use.

[0009] The present invention is further configured such that a female hook and loop fastener is adhered to one side of the rubber pad, and a female hook and loop fastener is adhered to the other side of the rubber pad, wherein the female hook and loop fastener and the female hook and loop fastener are bonded together.

[0010] The above technical solution uses a combination of male and female hook and loop fasteners to secure the rubber pad, preventing it from falling off during use.

[0011] The present invention is further configured such that the bottom of the inclined plate is in close contact with the surface of the first limiting plate, and the surface of the centrifuge tube body is provided with scale lines.

[0012] The above technical solution improves the stability of the sealing cover by setting the connection between the inclined plate and the first limiting plate.

[0013] In summary, this utility model has the following beneficial effects: 1. The rubber pad effectively protects the fingers of staff by utilizing its softness, improving staff comfort and increasing the efficiency of centrifuge tubes. 2. The male and female hook and loop fasteners facilitate locking the rubber pad and prevent it from falling off during use. The first limiting plate, the inclined plate, and the second limiting plate facilitate limiting the sealing cover and prevent it from loosening and falling off during use. Attached Figure Description

[0014] Figure 1 This is a schematic diagram of the overall structure of this utility model; Figure 2 This is the utility model Figure 1 A magnified view of section A in the image.

[0015] Reference numerals in the attached diagram: 1. Centrifuge tube body; 2. Sealing cap; 3. First limiting plate; 4. Second limiting plate; 5. Actuating plate; 6. Female hook and loop fastener; 7. Female hook and loop fastener; 8. Rubber pad; 9. Inclined plate. Detailed Implementation

[0016] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the protection scope of the present utility model.

[0017] Example 1: refer to Figure 1-2 The centrifuge tube for amino acid sample pretreatment includes a centrifuge tube body 1, which is made of high-purity polypropylene (PP) material. Its performance parameters include a temperature range of -20℃ to 121℃ (suitable for autoclaving) and resistance to common amino acid processing reagents (such as Tris buffer, methanol, acetonitrile, etc.). A sealing cap 2 is attached to the top of the centrifuge tube body 1. A lever 5 is adhered to the surface of the sealing cap 2, and a rubber pad 8 is attached to the bottom of the lever 5. The rubber pad 8 is made of food-grade silicone material resistant to chemical corrosion. The rubber pad 8 effectively protects the fingers of staff by utilizing its softness, improving staff comfort and increasing the efficiency of the centrifuge tube body 1.

[0018] Furthermore, a male hook and loop fastener 6 is attached to one side of the rubber pad 8, and a female hook and loop fastener 7 is attached to the other side of the rubber pad 8. The male hook and loop fastener 6 and the female hook and loop fastener 7 are bonded together, which facilitates locking the rubber pad 8 and prevents it from falling off during use.

[0019] Example 2: refer to Figure 1-2 The amino acid sample pretreatment centrifuge tube includes a first limiting plate 3 bonded to the bottom of the sealing cap 2. The first limiting plate 3 is made of medical-grade silicone with better elasticity. A second limiting plate 4 is bonded to the surface of the centrifuge tube body 1. An inclined plate 9 is bonded to the surface of the second limiting plate 4. The first limiting plate 3, the inclined plate 9 and the second limiting plate 4 facilitate the limiting of the sealing cap 2 and prevent the sealing cap 2 from loosening and falling off during use.

[0020] Furthermore, the bottom of the inclined plate 9 is in close contact with the surface of the first limiting plate 3, and the surface of the centrifuge tube body 1 is provided with graduation lines with a graduation accuracy of 0.1mL and a range of 0~15mL, "compliant with GB / T 21171-2007 Laboratory Centrifuge Tube Standard", ensuring quantitative accuracy. By setting the connection relationship between the inclined plate 9 and the first limiting plate 3, the stability of limiting the sealing cap 2 is improved.

[0021] Brief description of usage: (e.g.) Figure 1-2 As shown, this is the state after the centrifuge tube body 1 is sealed. When it is necessary to open the sealing cover 2, the user presses the first limiting plate 3. Since the first limiting plate 3 is made of plastic, it has a certain degree of softness and automatically retracts. The retraction of the first limiting plate 3 disengages it from the surface of the inclined plate 9. Then, the user places their fingers on the surface of the rubber pad 8. The rubber pad 8 effectively protects the operator's fingers by utilizing its softness, improving the operator's comfort and also improving the efficiency of the centrifuge tube body. Then, the user moves the rubber pad 8, which drives the actuating plate 5 to move. The upward movement of the actuating plate 5 will move the sealing cover 2, opening the sealing cover 2. When it is necessary to replace the rubber pad 8, the user tears off the hook and loop fastener 6, detaching it from the surface of the female hook and loop fastener 7. At this time, the limiting pressure of the rubber pad 8 is released, and the user can remove the rubber pad 8.

[0022] It is important to note that: it is recommended to check the stickiness of the Velcro and the aging of the silicone parts every 3 months and replace them in time; if the scale lines become blurred, the centrifuge tubes need to be replaced to ensure experimental accuracy. Parts have a life cycle and can be replaced during regular maintenance when they no longer meet the performance requirements. The deterioration of the performance of parts due to long-term use is not a design defect of this application.

[0023] It should be noted that, in this document, relational terms such as "first" and "second" are used only to distinguish one entity or operation from another, and do not necessarily require or imply any such actual relationship or order between these entities or operations. Furthermore, the terms "comprising," "including," or any other variations thereof are intended to cover non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements includes not only those elements but also other elements not expressly listed, or elements inherent to such a process, method, article, or apparatus. Without further limitations, an element defined by the phrase "comprising one..." does not exclude the presence of other identical elements in the process, method, article, or apparatus that includes said element.

[0024] Although embodiments of the present invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made to these embodiments without departing from the principles and spirit of the present invention, the scope of which is defined by the appended claims and their equivalents.

Claims

1. Amino acid sample pre-treatment centrifuge tube, comprising a centrifuge tube body (1), characterized in that: The centrifuge tube body (1) is covered with a sealing cap (2) at the top. A toggle plate (5) is attached to the surface of the sealing cap (2). A rubber pad (8) is attached to the bottom of the toggle plate (5).

2. The amino acid sample prep centrifuge tube of claim 1, wherein: The bottom of the sealing cap (2) is bonded with a first limiting plate (3), the surface of the centrifuge tube body (1) is bonded with a second limiting plate (4), and the surface of the second limiting plate (4) is bonded with an inclined plate (9).

3. The amino acid sample prep centrifuge tube of claim 1, wherein: The rubber pad (8) has a female hook and loop fastener (6) attached to one side and a female hook and loop fastener (7) attached to the other side. The female hook and loop fastener (6) and the female hook and loop fastener (7) are bonded together.

4. The amino acid sample prep centrifuge tube of claim 2, wherein: The bottom of the inclined plate (9) is in close contact with the surface of the first limiting plate (3), and the surface of the centrifuge tube body (1) is provided with scale lines.

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