An isolated extract for use in a sub- totipotent stem cell

Abstract

This utility model relates to the field of cell separation and extraction technology, specifically disclosing a separation and extraction device for sub-pluripotent stem cells, including: a centrifuge drum, a test tube rack movably connected inside the centrifuge drum, a fixing component provided on the inner surface of the test tube rack, and a protective frame fixedly connected to the outer surface of the test tube rack. A connecting block inside the fixed upright links the pressing block and the linkage rod. When the test tube rack is sleeved on the outside of the fixed upright, its central groove squeezes the fixing clamp, driving the fixing plate and the movable clamp. A push rod drives the linkage block to move down and compress the spring column. When the test tube rack slides to the middle position of the fixing clamp, the elasticity of the spring column pushes the linkage rod back to reset, so that the movable clamp drives the fixing clamp to push outward and clamp the outer surface of the test tube rack through the guide groove, realizing automatic fixation without tools, effectively improving the problems of cumbersome manual fixation and unstable clamping in traditional devices.

Description

Technical Field

[0001] This utility model relates to the field of cell separation and extraction technology, specifically to a separation and extraction device for sub-pluripotent stem cells. Background Technology

[0002] Sub-pluripotent stem cells are a type of adult stem cell with multipotent differentiation potential. Their differentiation capacity lies between that of pluripotent stem cells and multipotent stem cells. Under specific conditions, they can differentiate into various tissue and organ cells, such as nerve cells, cardiomyocytes, and hepatocytes. Compared to embryonic stem cells, sub-pluripotent stem cells have a wider range of sources (they can be obtained from various adult tissues), lower immunogenicity, and fewer ethical controversies, demonstrating significant application value in regenerative medicine, disease treatment, and other fields.

[0003] Existing traditional separation and extraction devices for sub-pluripotent stem cells mostly rely on manual screw fastening or snap-on clamps. Installation requires repeated adjustments using tools, making the process cumbersome and time-consuming, and unsuitable for efficient batch processing. Therefore, we propose a separation and extraction device for sub-pluripotent stem cells. Utility Model Content

[0004] The purpose of this utility model is to provide a separation and extraction device for sub-pluripotent stem cells, in order to solve the problem that existing traditional separation and extraction devices for sub-pluripotent stem cells mentioned in the background art mostly rely on manual screw fastening or snap-on clamps, and require repeated adjustments with tools during installation. The operation process is cumbersome and time-consuming, making it difficult to meet the needs of efficient batch processing.

[0005] To achieve the above objectives, the present invention provides the following technical solution: a separation and extraction device for sub-pluripotent stem cells, comprising: a centrifuge drum, a test tube rack movably connected inside the centrifuge drum, a fixing component provided on the inner surface of the test tube rack, and a protective frame fixedly connected to the outer surface of the test tube rack; The fixing assembly includes a fixed upright, which is a hollow cylindrical structure with a smooth inner wall, providing a smooth channel for the movement of the connecting block and the linkage rod. A connecting block is movably connected inside the fixed upright; the shape of the connecting block matches the internal cross-section of the fixed upright, allowing it to slide up and down tightly against the inner wall with minimal gap between them to prevent wobbling during movement. A linkage rod is fixedly connected to the bottom of the connecting block; the linkage rod is a solid rod with high strength and rigidity, stably transmitting the force of the connecting block. A linkage block is fixedly connected to the outer surface of the linkage rod, and the linkage block and linkage rod are fixedly connected by welding to ensure a firm connection that is not easily broken. The outermost ends of the linkage block... A push rod is fixedly connected to the side. The push rod is made of the same material as the linkage rod and has threaded structures at both ends for connection with anti-slip buckles. A movable clamp is movably connected to the side surface of the push rod. The movable clamp can rotate flexibly around the push rod. A guide groove is connected through the interior of the movable clamp. The length and angle of the guide groove are designed according to the movement trajectory of the movable clamp to ensure that the movable clamp will not deviate from the preset path during movement. A fixed plate is fixedly connected to the end of the movable clamp away from the linkage block. The fixed plate has a rectangular flat plate structure, which can increase the connection area with the fixed clamp and improve the stability of the connection. A fixed clamp is fixedly connected to the end of the fixed plate away from the movable clamp. The clamping surface of the fixed clamp is provided with a soft rubber pad, which can enhance the clamping force and avoid scratching the test tube rack.

[0006] The upper surface of the connecting block is fixedly connected to a pressing block. The top of the pressing block is arc-shaped, which conforms to the ergonomic principle and makes it easy for operators to press. Its surface is also provided with anti-slip texture to prevent the hand from slipping when pressing. The connecting block is connected through the top of the fixed pole and is fixedly connected between the pressing block and the linkage rod. The three form a whole and can move up and down synchronously.

[0007] The bottom of the linkage rod is fixedly connected to a limiting plate, which is a circular flat plate with a diameter larger than that of the linkage rod. This limiting plate restricts the upward movement of the linkage rod and prevents it from detaching from the fixed upright. A spring column is fixedly connected to the side of the limiting plate away from the linkage rod. A support base is fixedly connected to the bottom of the spring column. The support base is a circular base, and its bottom fits tightly against the inner bottom of the fixed upright, providing stable support for the spring column.

[0008] The support base is fixedly connected to the bottom of the fixed pole inside, and the two are connected by bolts for easy disassembly and replacement. A connecting plate is fixedly connected to the bottom of the fixed pole. The connecting plate is a circular flat plate with a diameter larger than that of the fixed pole, which can increase the connection area between the fixed pole and the centrifugal drum and improve the stability of the overall structure.

[0009] The linkage block has two sets, which are fixedly connected to the upper and lower surfaces of the linkage rod, making the force on the movable clamp more even. The movable clamp is circumferentially connected to the surface of the linkage block away from the linkage rod in four sets. The included angle between the four sets of movable clamps is equal, forming a symmetrical distribution, which can clamp the test tube rack from four directions and improve the fixation effect. The movable clamp is symmetrically clamped on both sides of the push rod in two sets, so that the force of the push rod on the movable clamp is balanced and the movable clamp is prevented from tilting due to uneven force. The two ends of the push rod are fixedly connected with anti-slip buckles. The anti-slip buckles are connected to the two ends of the push rod by threads. Their diameter is larger than the diameter of the push rod, which can effectively prevent the movable clamp from slipping off the two ends of the push rod.

[0010] The fixing clips are fixedly connected in two sets at the upper and lower ends of the fixing plate. The fixing clips hold the test tube rack in the slot at the center. The test tube rack is fixedly connected in two sets at the upper and lower ends of the protective frame.

[0011] This utility model has at least the following beneficial effects: 1. In use, this utility model uses a connecting block inside the fixed upright to link the pressing block and the linkage rod. When the test tube rack is sleeved on the outside of the fixed upright, the central slot presses the fixed clamp, which in turn drives the fixed plate and the movable clamp. The linkage block is driven to move down and compress the spring column by the push rod. When the test tube rack slides to the middle position of the fixed clamp, the elasticity of the spring column pushes the linkage rod back to its original position, so that the movable clamp drives the fixed clamp to push outward and clamp the outer surface of the test tube rack through the guide groove. This achieves automatic fixing without tools and effectively improves the problems of cumbersome manual fixing and unstable clamping in traditional devices.

[0012] 2. When in use, the protective frame is fixed to the outer surface of the test tube rack and is fixedly connected to the centrifuge drum with the bottom connecting plate of the fixed upright to form a rigid support. During centrifugation, the limiting plate and the support seat restrict the axial displacement of the test tubes, and the movable clamp constrains the fixed clamp to maintain vertical clamping through the guide groove. The buffer structure of the protective frame absorbs radial vibration and avoids the test tubes from shaking or colliding due to centrifugal force, effectively improving the problems of high sample loss and poor stability of traditional centrifugation devices. Attached Figure Description

[0013] Figure 1 This is a schematic diagram of the overall structure of this utility model; Figure 2 This is a schematic diagram of the connection structure between the test tube rack and the fixing component of this utility model; Figure 3 This is a side sectional view of the connection structure between the fixed upright and the connecting plate of this utility model. Figure 4 This is a top sectional view of the structural fixing pole of this utility model.

[0014] In the diagram: 1. Centrifuge drum; 2. Test tube rack; 3. Fixing assembly; 31. Fixing pole; 32. Connecting block; 33. Pressing block; 34. Linkage rod; 35. Linkage block; 36. Movable clamp; 37. Guide groove; 38. Push rod; 39. Anti-slip buckle; 310. Fixing plate; 311. Fixing clamp; 312. Limiting plate; 313. Spring column; 314. Support base; 315. Connecting plate; 4. Protective frame. Detailed Implementation

[0015] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the protection scope of the present utility model.

[0016] Please see Figure 1-4 This utility model provides a technical solution: a separation and extraction device for sub-pluripotent stem cells, comprising: a centrifuge drum 1, a test tube rack 2 movably connected inside the centrifuge drum 1, a fixing component 3 provided on the inner surface of the test tube rack 2, and a protective frame 4 fixedly connected to the outer surface of the test tube rack 2.

[0017] In use, the connecting block 32 inside the fixed upright 31 links the pressing block 33 and the linkage rod 34. When the test tube rack 2 is sleeved on the outside of the fixed upright 31, the central slot squeezes the fixed clamp 311, which drives the fixed plate 310 and the movable clamp 36. The linkage block 35 is driven to move down and compress the spring column 313 through the push rod 38. When the test tube rack 2 slides to the middle position of the fixed clamp 311, the elasticity of the spring column 313 pushes the linkage rod 34 back to reset, so that the movable clamp 36 drives the fixed clamp 311 to push outward and clamp the outer surface of the test tube rack 2 through the guide groove 37. This achieves automatic fixing without tools, effectively improving the problems of cumbersome manual fixing and unstable clamping in traditional devices, improving operating efficiency, and ensuring the stability of the test tubes during centrifugation, reducing the risk of sample loss due to clamping problems.

[0018] The fixing component 3 includes a fixing pole 31, a connecting block 32 is movably connected inside the fixing pole 31, a linkage rod 34 is fixedly connected to the bottom of the connecting block 32, a linkage block 35 is fixedly connected to the outer surface of the linkage rod 34, a push rod 38 is fixedly connected to both sides of the outermost end of the linkage block 35, a movable clamp 36 is movably connected to the side surface of the push rod 38, a guide groove 37 is connected through the interior of the movable clamp 36, a fixing plate 310 is fixedly connected to the end of the movable clamp 36 away from the linkage block 35, and a fixing clamp 311 is fixedly connected to the end of the fixing plate 310 away from the movable clamp 36.

[0019] The fixing component 3 includes a fixing rod 31, which is a hollow cylindrical structure with a smooth inner wall, providing a smooth channel for the movement of the connecting block 32 and the linkage rod 34. The fixing rod 31 is internally connected to the connecting block 32, the shape of which matches the internal cross-section of the fixing rod 31, allowing it to slide up and down tightly against the inner wall with minimal gap between them, preventing wobbling during movement. The bottom of the connecting block 32 is fixedly connected to the linkage rod 34, which is a solid rod with high strength and rigidity, stably transmitting the force of the connecting block 32. The outer surface of the linkage rod 34 is fixedly connected to the linkage block 35, which is welded to the linkage rod 34 to ensure a strong and durable connection. The outermost two sides of the linkage block 35 are fixedly connected to push rods 38, which are made of the same material as the linkage rod 34 and have threaded ends for connection with anti-slip buckles 39. A movable clamp 36 is movably connected to the side surface of the push rod 38. The movable clamp 36 can rotate flexibly around the push rod 38. A guide groove 37 is connected through the interior of the movable clamp 36. The length and angle of the guide groove 37 are precisely designed according to the movement trajectory of the movable clamp 36 to ensure that the movable clamp 36 will not deviate from the preset path during movement. A fixing plate 310 is fixedly connected to the end of the movable clamp 36 away from the linkage block 35. The fixing plate 310 has a rectangular flat plate structure, which can increase the connection area with the fixing clamp 311 and improve the stability of the connection. A fixing clamp 311 is fixedly connected to the end of the fixing plate 310 away from the movable clamp 36. The clamping surface of the fixing clamp 311 is provided with a soft rubber pad, which can enhance the clamping force and avoid scratching the test tube rack 2.

[0020] A pressing block 33 is fixedly connected to the upper surface of the connecting block 32. The top of the pressing block 33 is arc-shaped to facilitate pressing by the operator, and its surface is provided with anti-slip texture to prevent hand slippage during pressing. The connecting block 32 is connected through the top of the fixed upright 31 and is fixedly connected between the pressing block 33 and the linkage rod 34. The three form a whole and can move up and down synchronously.

[0021] A limiting plate 312 is fixedly connected to the bottom of the linkage rod 34. The limiting plate 312 is a circular flat plate with a diameter larger than that of the linkage rod 34. It can effectively limit the upward movement distance of the linkage rod 34 and prevent the linkage rod 34 from detaching from the fixed upright 31. A spring column 313 is fixedly connected to the side of the limiting plate 312 away from the linkage rod 34. A support base 314 is fixedly connected to the bottom of the spring column 313. The support base 314 is a circular base. Its bottom fits tightly with the inner bottom of the fixed upright 31, providing stable support for the spring column 313.

[0022] The support base 314 is fixedly connected to the bottom of the fixed pole 31. The two are connected by bolts, which facilitates disassembly and replacement. The bottom of the fixed pole 31 is fixedly connected to a connecting plate 315. The connecting plate 315 is a circular flat plate with a diameter larger than that of the fixed pole 31, which can increase the connection area between the fixed pole 31 and the centrifugal drum 1 and improve the stability of the overall structure.

[0023] Two sets of linkage blocks 35 are fixedly connected to the upper and lower surfaces of the linkage rod 34, making the force on the movable clamp 36 more even. Four sets of movable clamps 36 are circumferentially connected to the surface of the linkage block 35 away from the linkage rod 34. The included angles between the four sets of movable clamps 36 are equal, forming a symmetrical distribution, which can clamp the test tube rack 2 from four directions, improving the fixation effect. Two sets of movable clamps 36 symmetrically clamp both sides of the push rod 38, balancing the force exerted by the push rod 38 on the movable clamps 36 and preventing the movable clamps 36 from tilting due to uneven force. Anti-slip buckles 39 are fixedly connected to both ends of the push rod 38. The anti-slip buckles 39 are threaded to both ends of the push rod 38, and their diameter is larger than that of the push rod 38, effectively preventing the movable clamps 36 from slipping off the ends of the push rod 38.

[0024] Two sets of fixing clips 311 are fixedly connected to the upper and lower ends of the fixing plate 310. The distance between the two sets of fixing clips 311 is adapted to the thickness of the test tube rack 2, allowing it to fit tightly against the surface of the test tube rack 2 and enhance the clamping force. The fixing clips 311 clamp at the slot in the center of the test tube rack 2, and the edges of the slot are rounded to avoid wear on the fixing clips 311. Two sets of test tube racks 2 are fixedly connected to the upper and lower ends of the protective frame 4. The two sets of test tube racks 2 are parallel to each other and are fixed to the protective frame 4 with bolts. The number of bolts is reasonably set according to the size and weight of the test tube rack 2 to ensure a firm connection, while also facilitating the disassembly and replacement of the test tube rack 2, and making it convenient for later maintenance and upkeep.

[0025] When in use, insert the test tube containing the sample into the slot that runs through the outer surface of the test tube rack 2. At this time, the bottom of the test tube abuts against the upper surface of the connecting plate 315. The test tube rack 2 is movably sleeved on the outside of the fixed upright 31, and its upper and lower surfaces are clamped and positioned by the fixing clamp 311 to ensure that the test tube will not move axially during centrifugation.

[0026] In the initial state, the pressing block 33 is in a naturally convex state due to the upward movement of the linkage rod 34 driven by the connecting block 32. At this time, the linkage block 35 is located in the middle of the fixed upright 31, the pushing rod 38 retracts inward, the movable clamp 36 is linked with the pushing rod 38 through the guide groove 37, and the fixed clamp 311 is in an open state, which facilitates the installation of the test tube rack 2. When installing the test tube rack 2, the test tube rack 2 is slowly fitted onto the outer surface of the fixed upright 31 from top to bottom. During the downward pressing of the test tube rack 2, the central slot of the test tube rack 2 will gradually squeeze the upper surface of the fixed clamp 311, causing the fixed clamp 311 to push the fixed plate 310 inward. Since the fixed plate 310 is fixedly connected between the fixed clamp 311 and the movable clamp 36, and the push rod 38 inside the movable clamp 36 is fixedly connected to both ends of the linkage block 35, when the fixed clamp 311 pushes the linkage block 35 to move through the push rod 38, the linkage block 35 will drive the limiting plate 312 to squeeze the spring column 313 through the linkage rod 34, causing the spring column 313 to undergo compression deformation and store elastic potential energy.

[0027] When the test tube rack 2 slides to the middle of the two sets of fixing clamps 311, the squeezing force of the test tube rack 2 on the fixing clamps 311 disappears. Under the action of the elastic potential energy of the spring column 313, the linkage rod 34 will be pushed upward, thereby driving the linkage block 35 to reset. During the reset process of the linkage block 35, the fixing clamp 311 will be pushed outward through the fixed connection between the movable clamp 36 and the fixing plate 310, so that the fixing clamp 311 is tightly clamped on the outer surface of the test tube rack 2, realizing the stable clamping and fixing of the test tube rack 2. This ensures that the test tube rack 2 will not shake or shift when the centrifuge drum 1 rotates at high speed, and ensures that the sub-pluripotent stem cell samples can be successfully separated and extracted.

[0028] Subsequently, after the centrifugation operation is completed, the operator presses the pressing block 33 by hand, causing the pressing block 33 to drive the linkage rod 34 downward through the connecting block 32. The downward movement of the linkage rod 34 causes the linkage block 35 to move downward synchronously, thereby pulling the fixing clamp 311 inward and releasing the fixing clamp 311 from its limiting and fixing effect on the test tube rack 2. Finally, under the elastic action of the spring column 313, the fixing clamp 311 will automatically reset for future use. The entire operation process is simple and convenient, requiring no tools and improving work efficiency.

[0029] It should be noted that, in this document, relational terms such as "first" and "second" are used only to distinguish one entity or operation from another, and do not necessarily require or imply any such actual relationship or order between these entities or operations. Furthermore, the terms "comprising," "including," or any other variations thereof are intended to cover non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements includes not only those elements but also other elements not expressly listed, or elements inherent to such process, method, article, or apparatus.

[0030] Although embodiments of the present invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made to these embodiments without departing from the principles and spirit of the present invention, the scope of which is defined by the appended claims and their equivalents.

Claims

1. An isolated extract for use in a sub- totipotent stem cell, comprising: A centrifuge drum, characterized in that: a test tube rack is movably connected inside the centrifuge drum, a fixing component is provided on the inner surface of the test tube rack, and a protective frame is fixedly connected to the outer surface of the test tube rack; The fixing assembly includes a fixed upright, a connecting block is movably connected inside the fixed upright, a linkage rod is fixedly connected to the bottom of the connecting block, a linkage block is fixedly connected to the outer surface of the linkage rod, a push rod is fixedly connected to both sides of the outermost end of the linkage block, a movable clamp is movably connected to the side surface of the push rod, a guide groove is connected through the interior of the movable clamp, a fixing plate is fixedly connected to the end of the movable clamp away from the linkage block, and a fixing clamp is fixedly connected to the end of the fixing plate away from the movable clamp.

2. The isolated extract of claim 1, wherein: A pressing block is fixedly connected to the upper surface of the connecting block, the connecting block is connected through the top of the fixed upright, and the connecting block is fixedly connected between the pressing block and the linkage rod.

3. The isolated extract of claim 1, wherein: A limiting plate is fixedly connected to the bottom of the linkage rod, and a spring column is fixedly connected to the side of the limiting plate away from the linkage rod. A support base is fixedly connected to the bottom of the spring column.

4. The isolated extract of claim 3, wherein: The support base is fixedly connected to the bottom of the fixed upright, and a connecting plate is fixedly connected to the bottom of the fixed upright, which is fixedly connected to the inside of the centrifugal drum.

5. The isolated extract of claim 1, wherein: The linkage block is provided in two sets and is fixedly connected to the upper and lower end surfaces of the linkage rod respectively. The movable clamp is circumferentially connected to the side surface of the linkage block away from the linkage rod in four sets. The movable clamp is symmetrically clamped on both sides of the push rod in two sets. The two ends of the push rod are fixedly connected with anti-slip buckles.

6. The isolated extract of claim 1, wherein: The fixing clips are fixedly connected in two sets to the upper and lower ends of the fixing plate. The fixing clips are clamped at the slot in the center of the test tube rack. The test tube rack is fixedly connected in two sets to the upper and lower end surfaces of the protective frame.

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