A PCR Array Detection Kit Based on 96-Well Plates

Abstract

This invention belongs to the field of reagent kit technology, specifically relating to a PCR array detection kit based on a 96-well plate. The kit includes a rectangular plate with a first circular hole inside. A reservoir tube is fixedly connected to the inner wall of the first circular hole, and a cap is fitted onto the surface of the reservoir tube. Second circular holes are formed on both the front and back sides of the cap, and a first cylinder is fixedly connected to the inner wall of each second circular hole. Through the coordination of the rectangular plate, the first circular hole, the reservoir tube, the cap, the second circular hole, the first cylinder, the positioning groove, the support block, the rectangular cylinder, the rectangular ring, and the trapezoidal groove, the user can separate the caps of the same row from their corresponding reservoir tubes by pulling the rectangular ring. This allows multiple reservoir tubes on the kit to be opened simultaneously or individually, making the kit more convenient to transport and use.

Description

Technical Field

[0001] This invention belongs to the field of reagent kit technology, specifically relating to a PCR array detection kit based on a 96-well plate. Background Technology

[0002] PCR arrays (polymerase chain reaction chips) are a high-throughput detection technology based on the principle of quantitative real-time PCR (qPCR), which can simultaneously and rapidly analyze the expression levels of multiple genes (tens to hundreds). They are widely used in gene expression profiling, signaling pathway research, disease biomarker screening, and drug target validation.

[0003] Currently, in PCR array assays, reagents are mostly stored in 96-well plates. Existing 96-well plates have 96 wells, each with a cap for sealing, allowing each well to be individually sealed. For example, a 96-well plate disclosed in Chinese Patent Publication No. CN222469099U for preventing cross-contamination uses this independent cap structure. However, in practice, when injecting reagents into the wells, only one cap can be opened at a time. This means users must open each cap individually when injecting multiple reagents, making the process cumbersome, time-consuming, and labor-intensive. Another approach is to use an aluminum foil sealing film, a transparent film using gel, widely used in experiments with 96-well plates. Its main function is to ensure a close fit between the sealing film and the plate to prevent liquid evaporation. However, in practice, when injecting test reagents into the wells, the sealing film needs to be torn off first. Because the film is very thin and sticky, the tearing process varies from person to person, meaning the entire film cannot be torn off in one go, leading to cross-contamination. Furthermore, when selecting a specific location for sample addition, the single-piece film is very inconvenient. Summary of the Invention

[0004] The purpose of this invention is to provide a PCR array detection kit based on a 96-well plate, which solves the problem that when injecting test reagents into the wells, users can only open one cap at a time, requiring them to open each cap individually when injecting multiple test reagents, resulting in a cumbersome, time-consuming, and labor-intensive operation. Additionally, it solves the problem of cross-contamination during the tearing process when using a single sealing film.

[0005] The specific technical solution adopted by this utility model is as follows: A PCR array detection kit based on a 96-well plate includes a rectangular plate. A first circular hole is formed inside the rectangular plate. A liquid storage tube is fixedly connected to the inner wall of the first circular hole. A cap is fitted onto the surface of the liquid storage tube. Second circular holes are formed on both the front and rear sides of the cap. A first cylinder is fixedly connected to the inner wall of the second circular hole. A positioning groove is formed on the upper surface of the liquid storage tube. The inner wall of the positioning groove is fitted to the surface of the first cylinder. A support block is fixedly connected to the upper surface of the rectangular plate. A rectangular cylinder is fixedly connected to the upper surface of the support block. A rectangular ring is fitted to the inner wall of the rectangular cylinder. A trapezoidal groove is formed on the upper surface of the rectangular ring. The inner wall of the trapezoidal groove is fitted to the surface of the first cylinder. A box is fitted onto the surface of the rectangular plate. The inner wall of the box is fitted to the bottom of the liquid storage tube.

[0006] The present invention is further configured such that a U-shaped plate is fixedly connected to the upper surface of the rectangular plate, and a cylinder is fitted to both the left and right sides of the inner wall of the U-shaped plate. A second cylinder is fixedly connected to the inner wall of the cylinder. A limiting groove is formed on the front side of the U-shaped plate, and the inner wall of the limiting groove is fitted to the surface of the second cylinder. A U-shaped column is fixedly connected to the front side of the cylinder, and a pressing column is fixedly connected to both the inner wall of the U-shaped column and the front side of the cylinder. The bottom of the pressing column is fitted to the cap.

[0007] The present invention is further configured such that a rubber sealing block is fitted to the inner wall of the liquid storage tube, a groove is formed on the upper surface of the rubber sealing block, the inner wall of the groove is fitted to the surface of the first cylinder, and the upper surface of the rubber sealing block is bonded to the inner wall of the cap.

[0008] The present invention is further configured such that the rubber sealing block is located in the middle of the cap, and the bottom surface of the rubber sealing block is flush with the bottom surface of the cap.

[0009] The present invention is further configured such that both the left and right sides of the trapezoidal groove are inclined surfaces, and the depth of the trapezoidal groove is greater than the distance between the bottom surface of the rubber sealing block and the top surface of the liquid storage tube.

[0010] The present invention is further configured such that a marking ring is fixedly connected to the inner wall of the liquid storage tube, and the marking ring is located below the rubber sealing block.

[0011] The technical effects achieved by this utility model are as follows: This invention relates to a PCR array detection kit based on a 96-well plate. Through the combination of a rectangular plate, a first circular well, a reservoir tube, a cap, a second circular well, a first cylinder, a positioning groove, a support block, a rectangular tube, a rectangular ring, and a trapezoidal groove, the user can separate the caps of each row from their corresponding reservoir tubes by pulling the rectangular ring. This allows multiple reservoir tubes on the kit to be opened simultaneously or individually, making the kit more convenient to use. Furthermore, the independent cap and rectangular ring linkage design allows for precise control of the opening range, preventing unused reservoir tubes from being exposed and reducing the risk of cross-contamination.

[0012] This utility model discloses a PCR array detection kit based on a 96-well plate. Through the cooperation of a U-shaped plate, a cylinder, a second cylinder, a limiting groove, a U-shaped column, and pressing columns, the user can press all the caps on the detection kit at once by inserting the second cylinder into the limiting groove and rotating the U-shaped column. This makes it more convenient for the user to tighten all the caps on the detection kit. Attached Figure Description

[0013] Figure 1 This is a front view of the structure of this utility model; Figure 2 yes Figure 1 Enlarged view of point A in the middle; Figure 3 This is a top view of the structure of this utility model; Figure 4 yes Figure 3 Enlarged view at point B in the middle; Figure 5 yes Figure 3 Sectional view at CC; Figure 6 yes Figure 5 Enlarged view at point D; Figure 7 This is a top view of the rectangular plate in this utility model; Figure 8 This is a front view of the liquid storage tube in this utility model; Figure 9 This is a top view of the liquid storage tube in this utility model; Figure 10 This is a front view of the cap in this utility model; Figure 11 This is a bottom view of the cap in this utility model; Figure 12 This is a front view of the U-shaped plate in this utility model; Figure 13 This is a left view of the U-shaped plate in this utility model; Figure 14This is a top view of the cylindrical tube, the second cylindrical tube, the U-shaped column, and the pressing column in this utility model; Figure 15 This is a front view of the rubber sealing block in this utility model; Figure 16 This is a top view of the rubber sealing block in this utility model.

[0014] The attached diagram lists the components represented by each number as follows: 1. Rectangular plate; 2. First circular hole; 3. Liquid storage tube; 4. Cap; 5. Second circular hole; 6. First cylinder; 7. Positioning groove; 8. Support block; 9. Rectangular tube; 10. Rectangular ring; 11. Trapezoidal groove; 12. Box body; 13. U-shaped plate; 14. Cylinder; 15. Second cylinder; 16. Limiting groove; 17. U-shaped column; 18. Pressing column; 19. Rubber sealing block; 20. Groove; 21. Marking ring. Detailed Implementation

[0015] To make the above-mentioned objectives, features and advantages of this utility model more apparent and understandable, the specific embodiments of this utility model will be described in detail below with reference to the accompanying drawings.

[0016] Many specific details are set forth in the following description in order to provide a full understanding of the present invention. However, the present invention may also be implemented in other ways different from those described herein. Those skilled in the art can make similar extensions without departing from the spirit of the present invention. Therefore, the present invention is not limited to the specific embodiments disclosed below.

[0017] like Figures 1 to 16 As shown, a PCR array detection kit based on a 96-well plate includes a rectangular plate 1. A first circular hole 2 is formed inside the rectangular plate 1. A liquid storage tube 3 is fixedly connected to the inner wall of the first circular hole 2. A cap 4 is fitted onto the surface of the liquid storage tube 3. Second circular holes 5 are formed on both the front and rear sides of the cap 4. A first cylinder 6 is fixedly connected to the inner wall of the second circular hole 5. A positioning groove 7 is formed on the upper surface of the liquid storage tube 3. The inner wall of the positioning groove 7 is in contact with the surface of the first cylinder 6. A support block 8 is fixedly connected to the upper surface of the rectangular plate 1. A rectangular cylinder 9 is fixedly connected to the upper surface of the support block 8. A rectangular ring 10 is fitted onto the inner wall of the rectangular cylinder 9. A trapezoidal groove 11 is formed on the upper surface of the rectangular ring 10. The inner wall of the trapezoidal groove 11 is in contact with the surface of the first cylinder 6. A box body 12 is fitted onto the surface of the rectangular plate 1. The inner wall of the box body 12 is in contact with the bottom of the liquid storage tube 3.

[0018] The inner wall of the liquid storage tube 3 is fitted with a rubber sealing block 19. The upper surface of the rubber sealing block 19 has a groove 20. The inner wall of the groove 20 is fitted with the surface of the first cylinder 6. The upper surface of the rubber sealing block 19 is bonded to the inner wall of the cap 4. The rubber sealing block 19 is located in the middle of the cap 4. The bottom surface of the rubber sealing block 19 is flush with the bottom surface of the cap 4. The left and right sides of the trapezoidal groove 11 are both inclined surfaces. The depth of the trapezoidal groove 11 is greater than the distance between the bottom surface of the rubber sealing block 19 and the top surface of the liquid storage tube 3.

[0019] A marking ring 21 is fixedly connected to the inner wall of the liquid storage tube 3, and the marking ring 21 is located below the rubber sealing block 19.

[0020] It should be noted that the cap 4 and the rubber sealing block 19 can seal the opening at the top of the liquid storage tube 3. The cap 4 on the liquid storage tube 3 can be positioned by the cooperation of the positioning groove 7 and the first cylinder 6. The rectangular ring 10 can only move left and right by the rectangular tube 9. When the rectangular ring 10 moves horizontally, the second cylinder 15 can move upward by the inclined surface on the trapezoidal groove 11. When the second cylinder 15 is misaligned with the trapezoidal groove 11, the cap 4 and the rubber sealing block 19 are separated from the liquid storage tube 3. Then, the user can separate the caps 4 in the same row from the corresponding liquid storage tubes 3 by pulling the rectangular ring 10. The marking ring 21 allows the user to intuitively judge the amount of reagent in the liquid storage tube 3.

[0021] like Figures 1 to 14 As shown, a U-shaped plate 13 is fixedly connected to the upper surface of the rectangular plate 1. A cylinder 14 is attached to both the left and right sides of the inner wall of the U-shaped plate 13. A second cylinder 15 is fixedly connected to the inner wall of the cylinder 14. A limiting groove 16 is opened on the front of the U-shaped plate 13. The inner wall of the limiting groove 16 is attached to the surface of the second cylinder 15. A U-shaped column 17 is fixedly connected to the front of the cylinder 14. A pressing column 18 is fixedly connected to both the inner wall of the U-shaped column 17 and the front of the cylinder 14. The bottom of the pressing column 18 is attached to the cap 4.

[0022] It should be noted that when the second cylinder 15 is inserted into the limiting groove 16, the U-shaped plate 13, the cylinder 14, the limiting groove 16 and the second cylinder 15 cooperate to make the U-shaped column 17 rotate only around the second cylinder 15. When the user inserts the second cylinder 15 into the limiting groove 16 and rotates the U-shaped column 17, the multiple pressing columns 18 in the U-shaped column 17 can press all the caps 4 on the test kit at once, making it more convenient for the user to press all the caps 4 on the test kit.

[0023] The working principle of this utility model is as follows: When it is necessary to open a single liquid storage tube 3, the opening at the top of the liquid storage tube 3 can be opened by removing the cap 4 from the corresponding liquid storage tube 3. When it is necessary to open the liquid storage tubes 3 in the same row, the rectangular ring 10 is pulled to slide inside the rectangular cylinder 9. Then, through the inclined surface on the trapezoidal groove 11, the second cylinder 15 can move upward when the rectangular ring 10 moves. When the second cylinder 15 is misaligned with the trapezoidal groove 11, the cap 4 and the rubber sealing block 19 are separated from the liquid storage tube 3. At this time, all the liquid storage tubes 3 in the same row can be opened.

[0024] When it is necessary to press all the caps 4 on the test kit, first insert the second cylinder 15 into the limiting groove 16, and make both the left and right sides of the cylinder 14 contact the inner wall of the U-shaped plate 13. At this time, through the cooperation of the U-shaped plate 13, the cylinder 14, the limiting groove 16 and the second cylinder 15, the U-shaped column 17 can only rotate around the second cylinder 15. Then, by rotating the U-shaped column 17 around the second cylinder 15, the multiple pressing columns 18 inside the U-shaped column 17 can press all the caps 4 on the test kit at one time, which makes it more convenient for the user to press all the caps 4 on the test kit.

[0025] When the rectangular plate 1 is installed on the machine, the U-shaped column 17 must be removed from the rectangular plate 1 before it can be installed on the machine.

[0026] The above description is merely a preferred embodiment of this utility model. It should be noted that those skilled in the art can make various improvements and modifications without departing from the principle of this utility model, and these improvements and modifications should also be considered within the scope of protection of this utility model. Structures, devices, and operating methods not specifically described or explained in this utility model, unless otherwise specified or limited, shall be implemented using conventional methods in the field.

Claims

1. A 96-well plate-based PCR Array detection kit, characterized in that: The device includes a rectangular plate (1), with a first circular hole (2) inside the rectangular plate (1). A liquid storage tube (3) is fixedly connected to the inner wall of the first circular hole (2). A cap (4) is fitted onto the surface of the liquid storage tube (3). Second circular holes (5) are provided on both the front and rear sides of the cap (4). A first cylinder (6) is fixedly connected to the inner wall of the second circular hole (5). A positioning groove (7) is provided on the upper surface of the liquid storage tube (3). The inner wall of the positioning groove (7) is attached to the surface of the first cylinder (6). The rectangular plate (1) is fixedly connected to a support block (8), and the support block (8) is fixedly connected to a rectangular cylinder (9). A rectangular ring (10) is fitted to the inner wall of the rectangular cylinder (9). A trapezoidal groove (11) is opened on the upper surface of the rectangular ring (10). The inner wall of the trapezoidal groove (11) is fitted to the surface of the first cylinder (6). A box body (12) is fitted to the surface of the rectangular plate (1). The inner wall of the box body (12) is fitted to the bottom of the liquid storage tube (3).

2. The 96-well plate-based PCR Array detection kit according to claim 1, characterized in that: A U-shaped plate (13) is fixedly connected to the upper surface of the rectangular plate (1). A cylinder (14) is attached to both the left and right sides of the inner wall of the U-shaped plate (13). A second cylinder (15) is fixedly connected to the inner wall of the cylinder (14). A limiting groove (16) is opened on the front of the U-shaped plate (13). The inner wall of the limiting groove (16) is attached to the surface of the second cylinder (15). A U-shaped column (17) is fixedly connected to the front of the cylinder (14). A pressing column (18) is fixedly connected to both the inner wall of the U-shaped column (17) and the front of the cylinder (14). The bottom of the pressing column (18) is attached to the cap (4).

3. The 96-well plate-based PCR Array detection kit according to claim 1, characterized in that: The inner wall of the liquid storage tube (3) is fitted with a rubber sealing block (19), and the upper surface of the rubber sealing block (19) is provided with a groove (20). The inner wall of the groove (20) is fitted with the surface of the first cylinder (6), and the upper surface of the rubber sealing block (19) is bonded to the inner wall of the cap (4).

4. The 96-well plate-based PCR Array detection kit according to claim 3, characterized in that: The rubber sealing block (19) is located in the middle of the cap (4), and the bottom surface of the rubber sealing block (19) is flush with the bottom surface of the cap (4).

5. The 96-well plate-based PCR Array detection kit according to claim 3, characterized in that: The trapezoidal groove (11) has inclined surfaces on both the left and right sides, and the depth of the trapezoidal groove (11) is greater than the distance between the bottom surface of the rubber sealing block (19) and the top surface of the liquid storage tube (3).

6. The 96-well plate-based PCR Array detection kit according to claim 1, characterized in that: A marking ring (21) is fixedly connected to the inner wall of the liquid storage tube (3), and the marking ring (21) is located below the rubber sealing block (19).

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