An immunochromogenic reagent staining kit suitable for immunohistochemistry

Abstract

This invention discloses an immunochromatographic staining kit suitable for immunohistochemistry, relating to the field of reagent kit technology. The kit includes a kit body and multiple reagent bottles, all housed within the kit body. Each reagent bottle includes a cap and a body, which are rotatably connected to seal the bottle. The kit body incorporates a shock-absorbing component with multiple placement positions for the reagent bottles to ensure stability. In practical use, this invention, through optimized shock-absorbing components and reagent bottle structure, achieves stable, safe, and efficient operation of the reagent bottles. The kit is compatible with both manual and automated scenarios, significantly improving the efficiency, accuracy, and safety of immunohistochemistry experiments. It is suitable for various scenarios such as scientific research, clinical diagnosis, and high-throughput drug development, and has broad application value.

Description

Technical Field

[0001] This utility model relates to the field of reagent kit technology, specifically to an immunochromatographic staining reagent kit suitable for immunohistochemistry. Background Technology

[0002] Immunohistochemistry (IHC) is a laboratory detection technique based on the principle of specific antigen-antibody binding. It involves the binding of antibody-labeled chromogenic agents, such as fluorescein, enzymes, or metal ions, to target antigens in tissues or cells, resulting in color development. This allows for the localization, qualitative analysis, and relative quantification of antigens such as proteins and peptides within tissues or cells. Immunohistochemistry is an indispensable tool in modern medicine. By accurately detecting protein expression, it provides crucial evidence for disease diagnosis, treatment selection, and prognostic assessment. Its application is particularly prominent in tumor diagnosis and treatment, significantly improving the scientific rigor and individualization of clinical decisions. Immunohistochemical staining kits are key tools in IHC experiments, used for color development and signal amplification. Their core function is to visualize target antigens in tissues or cells through specific chemical reactions or fluorescent labeling. Their core function is to convert the antigen-antibody binding site into observable chromogenic precipitation or fluorescent signals through chemical or optical signal amplification systems, thereby assisting in pathological diagnosis, disease research, and molecular biological analysis.

[0003] However, in the existing technology, the immunochromatographic reagent kits currently on the market are prone to damage during transportation and use. The kits lack shockproof components or the shockproof components are made of poor materials, which cannot effectively protect the reagent bottles. In addition, the bottle caps in the existing technology use multi-layer sealing structures or special locking devices, which are not convenient and efficient to open during use, affecting the efficiency of the experiment. Utility Model Content

[0004] In view of the problems in the related technologies, this utility model proposes an immunochromogenic reagent staining kit suitable for immunohistochemistry, so as to overcome the above-mentioned technical problems existing in the existing related technologies.

[0005] Therefore, the specific technical solution adopted by this utility model is as follows:

[0006] An immunochromogenic staining kit for immunohistochemistry includes a kit body and multiple reagent bottles, all of which are placed inside the kit body. Each reagent bottle includes a cap and a body, which are rotatably connected to seal the reagent bottle. The kit body is equipped with a shock-absorbing component, which has multiple placement positions for the reagent bottles to ensure their stability.

[0007] The reagent vials contain: an endogenous peroxidase inhibitor vial; a reaction enhancement solution vial; a DAB concentrated chromogenic solution vial; a DAB substrate buffer vial; and a hematoxylin counterstain vial. These vials increase the sensitivity of the detection, and the enhancement solution further improves the blocking of nonspecificity.

[0008] This kit is compatible with both manual and fully automated immunohistochemistry staining, allowing customers with different needs to choose freely. The kit body is equipped with multiple placement positions to securely hold multiple reagent bottles, preventing them from shaking or colliding inside the box, thus preventing damage or leakage. This ensures the integrity and safety of the reagents during transportation and storage, while also allowing users to quickly access the required reagents, improving experimental efficiency and accuracy.

[0009] Furthermore, the reagent bottle includes a body and a cap. The body is a three-dimensional cylindrical shape, allowing for a more compact arrangement within the storage area. Compared to other irregularly shaped bodies, the cylindrical structure reduces gaps between bottles during placement. The end of the body features an opening for easy pipette tip insertion, enabling rapid and accurate reagent aspiration, saving time, improving experimental efficiency, reducing the chance of reagent contact with external elements, preventing impurities from entering, ensuring reagent purity, and extending shelf life.

[0010] Furthermore, the bottle cap has a protrusion that corresponds to the opening and matches the opening to seal the bottle body. This tight seal can effectively prevent reagent leakage during storage and transportation, ensuring the integrity of the reagent. The matching opening and protrusion allow operators to complete the sealing and opening of the reagent bottle more quickly.

[0011] Furthermore, a hinge is provided on one side of the bottle cap, which connects the bottle cap to the bottle body, allowing the bottle cap to rotate relative to the bottle body. Since the bottle cap is connected to the bottle body, there is no need to worry about losing the bottle cap during use. The hinge connection makes the alignment of the bottle cap and the bottle body more precise, enhances the sealing effect, and extends the shelf life of the reagent.

[0012] Furthermore, a fixing structure is provided on the bottle body, which is fixedly connected to the bottle body. The bottle cap can be hung on the fixing hook to prevent loss, ensure it is always available, save reagent kit space, make the internal layout more compact, can accommodate more reagent bottles, and improve the overall space utilization rate.

[0013] Furthermore, the shock-absorbing components are made of aluminum foam, which physically cushions the reagent bottles to prevent collisions. The porous structure inside the aluminum foam gives it a strong energy absorption capacity, enabling it to quickly absorb and disperse a large amount of energy upon impact, preventing the reagent bottles from shaking or shifting during placement or movement, thus further improving the stability and safety of the reagent kit.

[0014] Furthermore, the placement of the shock-absorbing components features a hole or groove structure to ensure the stability of the reagent bottles, prevent shaking or collisions during use or transportation, avoid damage to the reagent bottles or reagent leakage, ensure the integrity and safety of the reagent kit, and facilitate the placement and retrieval of reagent bottles. Users can quickly and accurately retrieve the required reagent bottles, improving experimental efficiency.

[0015] Furthermore, the height of the shockproof component is less than the height of the reagent kit itself, providing space for other components or accessories inside the kit, optimizing the spatial layout, increasing the overall capacity, making it convenient for users to pick up and put down reagent bottles without being obstructed or scratched by the inner lining, reducing the difficulty of operation and the risk of reagent bottle damage.

[0016] Furthermore, the kit is compatible with both manual and fully automated immunohistochemical staining instruments. Regardless of the method, the reagent composition and reaction conditions remain the same, ensuring comparability of experimental results. This expands the kit's application range, enabling its use in more diverse experimental environments. This compatibility not only facilitates user operation but also contributes to the standardization and normalization of immunohistochemical techniques.

[0017] The beneficial effects of this utility model are as follows:

[0018] 1. The reagent bottle of this utility model is rotatably connected to the bottle body by the bottle cap, and the bottle cap is provided with a protrusion corresponding to the opening to form a tight fit and seal, which realizes a dual improvement in operation efficiency and reagent safety, and is suitable for testing scenarios with high requirements for reagent purity, stability and operation convenience.

[0019] 2. This utility model, through the layout of foamed aluminum and shockproof components, effectively prevents reagent bottles from shaking and shifting during transportation and storage, thereby reducing the risk of collision and tipping caused by shaking, preventing reagent leakage due to reagent bottle breakage, and improving the safety of reagent kits during transportation and storage.

[0020] 3. The reagent kit of this utility model is compatible with both manual and fully automated immunohistochemical staining. The reagent kit is compatible with both operation methods, and a unified quality control standard and process can be established. It is applicable to most fully automated instruments on the market. The reagent kit reduces production costs, saves expenses for medical institutions and testing institutions, and improves economic efficiency. Attached Figure Description

[0021] To more clearly illustrate the technical solutions in the embodiments of this utility model or the prior art, the drawings used in the embodiments will be briefly introduced below. Obviously, the drawings described below are only some embodiments of this utility model. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort.

[0022] Figure 1 This is a schematic diagram of the structure of an immunochromogenic reagent staining kit suitable for immunohistochemistry according to an embodiment of the present invention;

[0023] Figure 2 This is a schematic diagram of the reagent bottle cap structure of an immunochromogenic reagent staining kit suitable for immunohistochemistry according to an embodiment of the present invention;

[0024] Figure 3 This is a schematic diagram of the reagent bottle structure of an immunochromogenic staining kit suitable for immunohistochemistry, according to an embodiment of the present invention.

[0025] In the picture:

[0026] 1. Reagent kit body; 2. Reagent bottle; 21. Bottle body; 211. Opening; 212. Fixing structure; 22. Bottle cap; 221. Protrusion; 3. Shockproof component; 31. Placement position. Detailed Implementation

[0027] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the protection scope of the present utility model.

[0028] like Figure 1 As shown in the embodiment of this utility model, an immunochromatographic staining reagent kit suitable for immunohistochemistry is provided, including a kit body 1 and multiple reagent bottles 2. All reagent bottles 2 are placed within the kit body 1. A shock-absorbing component 3 is provided within the kit body 1, and the shock-absorbing component 3 has multiple placement positions 31 for the reagent bottles 2. The placement positions 31 of the shock-absorbing component 3 have a perforated or grooved structure to ensure the stability of the reagent bottles 2. The shock-absorbing component 3 is made of aluminum foam, which physically cushions the reagent bottles 2 to prevent collisions. The height of the shock-absorbing component 3 is less than the height of the kit body 1. The shock-absorbing component 3, through its structured fixation to the aluminum foam and its physical cushioning design at the placement positions 31, optimizes the space utilization and ease of operation of the kit while ensuring the safety of the reagent bottles, achieving a dual improvement in operational efficiency and reagent safety.

[0029] like Figures 2-3 As shown, the reagent bottle 2 includes a bottle body 21 and a bottle cap 22. The bottle body 21 is a three-dimensional columnar shape, and the bottle cap 22 is rotatably connected to the bottle body 21. The end of the bottle body 21 has an opening 211, and the bottle cap 22 has a protrusion 221 corresponding to the opening 211, which matches the opening 211 and is used to seal the bottle body 21. A hinge is provided on one side of the bottle cap 22, and the bottle cap 22 is connected to the bottle body 21 through the hinge, so that the bottle cap 22 can rotate relative to the bottle body 21. The hinge structure ensures that the bottle cap 22 is always aligned with the bottle body 21, reducing the risk of seal failure due to misalignment of the bottle cap 22. A fixing structure 212 is provided on the bottle body 21, and the fixing structure 212 is fixedly connected to the bottle body 21 to fix the reagent bottle 2.

[0030] The kit is compatible with both manual and fully automated immunohistochemical staining instruments, allowing laboratories to flexibly allocate personnel and equipment according to their actual needs. For simple samples, manual operation can be completed quickly, while complex samples can be automated using the instrument. Rational allocation of experimental tasks and complementary operation methods improve overall efficiency. It meets the dual needs of laboratories for both manual handling of small batches of samples and high-throughput automated processing, exhibiting high compatibility and flexibility, adapting to multiple application scenarios, reducing reagent waste and equipment dependence, and facilitating multi-center research and clinical trials requiring multi-laboratory collaboration.

[0031] The above description is only a preferred embodiment of the present utility model and is not intended to limit the present utility model. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of the present utility model should be included within the protection scope of the present utility model.

Claims

1. An immunochromogenic reagent staining kit suitable for immunohistochemistry, characterized in that, The kit includes a reagent body (1) and multiple reagent bottles (2). The reagent bottles (2) are all placed in the reagent body (1). Each reagent bottle (2) includes a cap (22) and a body (21). The cap (22) and body (21) are rotatably connected to seal the reagent bottle (2). The reagent body (1) is equipped with a shockproof component (3). The shockproof component (3) has multiple placement positions (31) for the reagent bottles (2) to ensure the stability of the reagent bottles (2).

2. The immunochromogenic reagent staining kit for immunohistochemistry according to claim 1, characterized in that, The bottle body (21) is a three-dimensional columnar shape, and the end of the bottle body (21) is provided with an opening (211).

3. The immunochromogenic reagent staining kit for immunohistochemistry according to claim 1, characterized in that, The bottle cap (22) has a protrusion (221) corresponding to the opening (211) for sealing the bottle body (21).

4. The immunochromogenic reagent staining kit for immunohistochemistry according to claim 3, characterized in that, A hinge is provided on one side of the bottle cap (22), and the bottle cap (22) is connected to the bottle body (21) through the hinge, so that the bottle cap (22) can rotate relative to the bottle body (21).

5. The immunochromogenic reagent staining kit for immunohistochemistry according to claim 2, characterized in that, A fixing structure (212) is provided on the bottle body (21), and the fixing structure (212) is fixedly connected to the bottle body (21).

6. The immunochromogenic reagent staining kit for immunohistochemistry according to claim 1, characterized in that, The shock-absorbing component (3) is made of aluminum foam, which prevents the reagent bottle (2) from colliding through physical cushioning.

7. The immunochromogenic reagent staining kit for immunohistochemistry according to claim 6, characterized in that, The placement position (31) of the shockproof component (3) is a hole or groove structure to ensure the stability of the reagent bottle (2).

8. The immunochromogenic reagent staining kit for immunohistochemistry according to claim 7, characterized in that, The height of the shockproof component (3) is less than the height of the reagent kit body (1).

9. The immunochromogenic reagent staining kit for immunohistochemistry according to claim 1, characterized in that, The kit is compatible with both manual and fully automated immunohistochemical staining instruments.

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