A sheet tissue decellularization component

Abstract

This application provides a tissue decellularization assembly, relating to the field of decellularization devices. The tissue decellularization assembly includes a first clamping plate and a second clamping plate, a fixing member, and a housing. The first and second clamping plates are used to clamp the tissue sheet. The first and second clamping plates have a hollow center and through holes along their thickness. The fixing member has multiple slots on one side, distributed along its length, allowing one end of the first and second clamping plates holding the tissue sheet to be engaged in these slots. The housing holds the decellularization reagent. The fixing member is detachably connected to the housing and can be fixed to opposite sides within the housing. The distance between the fixing members on opposite sides is less than the length of the first and second clamping plates. This assembly clamps the tissue sheet to prevent curling, thereby improving the decellularization effect. Furthermore, the tissue decellularization assembly is easy to assemble and disassemble.

Description

Technical Field

[0001] This application relates to the field of decellularization devices, and more specifically, to a decellularization assembly for sheet tissue. Background Technology

[0002] Many biological tissue materials require decellularization before being used to manufacture medical devices, such as the submucosa of the small intestine (SIS) and porcine dermal matrix. Decellularization removes cells and other antigen molecules that may cause rejection reactions while preserving collagen, elastin, and functional proteins. This allows for the formation of specific functionalized tissues in vivo or in vitro, which can be used for tissue repair / reconstruction.

[0003] In existing decellularization processes, biological sheet tissues are typically placed directly in a decellularization reagent and shaken to remove cells. However, biological sheet tissues are prone to curling, resulting in poor decellularization effects. Utility Model Content

[0004] This application provides a sheet tissue decellularization assembly that can clamp the sheet tissue to prevent it from curling, thereby improving the decellularization effect. Furthermore, the sheet tissue decellularization assembly is easy to assemble and disassemble.

[0005] The embodiments of this application are implemented as follows: This application provides a sheet tissue decellularization assembly, including: The first clamping plate and the second clamping plate are used to clamp the sheet material; the middle of the first clamping plate and the second clamping plate are hollowed out, and the plate body has through holes along the thickness direction; The fastener has multiple slots on one side, which are distributed along the length of the fastener. One end of the first clamping plate and the second clamping plate, which hold the sheet material, can be engaged together in these slots. The box is used to hold decellularization reagents. The fixing member is detachably connected to the box and can be fixed to opposite sides inside the box. The distance between the fixing members on opposite sides is less than the length of the first clamping plate and the second clamping plate.

[0006] In one possible implementation, the through hole of the first clamping plate is a first through hole, and the through hole of the second clamping plate is a second through hole, and the first through hole and the second through hole are staggered (without any overlap in the thickness direction).

[0007] In one possible implementation, the fastener is connected and fixed to the bottom wall of the housing by a snap-fit ​​mechanism.

[0008] In one possible implementation, the bottom wall of the housing is provided with a protrusion, and the bottom end of the fastener is provided with a groove that matches the protrusion, and the protrusion can be engaged with the groove.

[0009] In one possible implementation, the protrusion is a trapezoidal body, and the outer diameter of the protrusion gradually increases from the end away from the bottom wall of the box to the end closer to the bottom wall of the box.

[0010] In one possible implementation, the slot for clamping at least one surface of the first clamping plate and the second clamping plate is provided with a spring piece; one end of the spring piece is connected to the side wall of the slot, and the other end is spaced apart from the side wall of the slot; when the first clamping plate and the second clamping plate are engaged in the slot, the spring piece can abut against the surface of the first clamping plate and / or the second clamping plate.

[0011] In one possible implementation, the upper end of the spring is connected to the side wall of the slot.

[0012] In one possible implementation, both the first clamping plate and the second clamping plate are polytetrafluoroethylene (PTFE) sheets.

[0013] The embodiments of this application have at least the following beneficial effects: The decellularization assembly for sheet tissue of this application features a first clamping plate and a second clamping plate that clamp the sheet tissue. After clamping, the first and second clamping plates are engaged together in the slots of a fixing member. These plates are then secured within the housing by fixing members on opposite sides, preventing the sheet tissue from curling and resulting in better decellularization. Furthermore, the fixing member is detachably connected to the housing, and the first and second clamping plates are engaged in the slots of the fixing member, facilitating assembly and disassembly. This allows for convenient clamping of the sheet tissue and easy, undamaged removal of the decellularized sheet tissue. Attached Figure Description

[0014] To more clearly illustrate the technical solutions of the embodiments of this application, the accompanying drawings used in the embodiments will be briefly introduced below. It should be understood that the following drawings only show some embodiments of this application and should not be regarded as a limitation of the scope. For those skilled in the art, other related drawings can be obtained based on these drawings without creative effort.

[0015] Figure 1 This is a schematic diagram of the box and fixing components of the sheet tissue decellularization assembly according to an embodiment of this application; Figure 2 This is a schematic diagram of the structure of the sheet tissue decellularization component after it has been clamped by the sheet tissue according to an embodiment of this application; Figure 3This is a schematic diagram of the first clamping plate according to an embodiment of this application; Figure 4 This is a schematic diagram of the second clamping plate according to an embodiment of this application; Figure 5 This is a schematic diagram showing the bottom view of the fastener in an embodiment of this application; Figure 6 This is a schematic diagram of the fastener according to an embodiment of this application.

[0016] Icons: 10-Sheet tissue decellularization assembly; 11-First clamping plate; 111-First through hole; 12-Second clamping plate; 121-Second through hole; 13-Fixing component; 131-Slot; 132-Groove; 133-Spring; 14-Box; 20-Sheet tissue. Detailed Implementation

[0017] To make the objectives, technical solutions, and advantages of the embodiments of this application clearer, the technical solutions of the embodiments of this application will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of this application, and not all embodiments. The components of the embodiments of this application described and shown in the accompanying drawings can generally be arranged and designed in various different configurations.

[0018] Therefore, the following detailed description of the embodiments of this application provided in the accompanying drawings is not intended to limit the scope of the claimed application, but merely to illustrate selected embodiments of the application. All other embodiments obtained by those skilled in the art based on the embodiments of this application without inventive effort are within the scope of protection of this application.

[0019] In the description of this application, it should be noted that the terms "upper," "lower," "inner," and "outer," etc., indicate the orientation or positional relationship based on the orientation or positional relationship shown in the accompanying drawings, or the orientation or positional relationship commonly used when the product is in use. They are used only for the convenience of describing this application and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation, and therefore should not be construed as a limitation on this application. In addition, the terms "first," "second," etc., are used only to distinguish descriptions and should not be construed as indicating or implying relative importance.

[0020] In the description of this application, it should also be noted that, unless otherwise expressly specified and limited, the terms "set," "install," and "connect" should be interpreted broadly. For example, they can refer to a fixed connection, a detachable connection, or an integral connection; they can refer to a direct connection or an indirect connection through an intermediate medium; and they can refer to the internal connection of two components. Those skilled in the art can understand the specific meaning of the above terms in this application based on the specific circumstances. Example

[0021] This embodiment provides a sheet tissue decellularization component 10. Please refer to [link to relevant documentation]. Figure 1 and Figure 2 It includes a first clamping plate 11, a second clamping plate 12, a fixing member 13 and a box 14. The box 14 is used to hold the decellularization reagent. The specific composition of the decellularization reagent can be selected according to the characteristics of the sheet tissue 20. This application does not make specific limitations.

[0022] The first clamping plate 11 and the second clamping plate 12 are hollowed out in the middle, and the plates have through holes along the thickness direction (see...). Figure 3 and Figure 4 The first clamping plate 11 and the second clamping plate 12 are used to clamp the sheet tissue 20. The hollow portion in the middle ensures that the clamped sheet tissue 20 is in full contact with the decellularization reagent. In addition, the through hole of the first clamping plate 11 is the first through hole 111, and the through hole of the second clamping plate 12 is the second through hole 121. The through holes allow the decellularization reagent to come into contact with the sheet tissue 20, thereby improving the decellularization effect.

[0023] For example, the first through hole 111 and the second through hole 121 are staggered, meaning that when the first clamping plate 11 and the second clamping plate 12 clamp the sheet tissue 20, the projections of the first through hole 111 and the second through hole 121 in the thickness direction of the sheet body do not overlap. This means that the side of the sheet tissue 20 corresponding to the first through hole 111 is supported by the second clamping plate 12, and the side corresponding to the second through hole 121 is supported by the first clamping plate 11. The first through hole 111 and the second through hole 121 allow the decellularization reagent to penetrate the sheet tissue 20 for a sufficient reaction, while the other side of the sheet tissue 20 corresponding to the first through hole 111 and the second through hole 121 is also supported, making the sheet tissue 20 less prone to deformation and providing a better decellularization effect. Optionally, the shape of the first through hole 111 and the second through hole 121 can be a round hole or a square hole, etc. It should be noted that in other embodiments, the first through hole 111 and the second through hole 121 can also be arranged in alignment, that is, when the first clamping plate 11 and the second clamping plate 12 clamp the sheet material 20, the projections of the first through hole 111 and the second through hole 121 in the thickness direction of the sheet material partially overlap or completely overlap.

[0024] The fastener 13 is used for installation inside the housing 14. The fastener 13 is detachably connected to the housing 14 and can be fixed on opposite sides inside the housing 14 for easy assembly and disassembly. The distance between the fasteners 13 on opposite sides is less than the length of the first clamping plate 11 and the second clamping plate 12.

[0025] Optionally, the fastener 13 is connected and fixed to the bottom wall of the housing 14 by a snap-fit ​​connection. For example, the bottom wall of the housing 14 is provided with a protrusion (not shown in the figure), and the bottom end of the fastener 13 is provided with a groove 132 that matches the protrusion (see reference). Figure 5 The protrusion can engage with the groove 132. By engaging the groove 132 at the bottom of the fastener 13 with the protrusion on the bottom wall of the housing 14, the fastener 13 can be connected and fixed inside the housing 14. When it is necessary to connect and fix the fastener 13 to the housing 14, simply align the groove 132 of the fastener 13 with the protrusion at the bottom of the housing 14 and install the fastener 13 on the protrusion; when it is necessary to remove the fastener 13, simply lift the fastener 13 to disengage the protrusion from the groove 132. Both assembly and disassembly are relatively convenient.

[0026] For example, the protrusion is a trapezoidal block, and its outer diameter gradually increases from the end furthest from the bottom wall of the housing 14 to the end closest to the bottom wall of the housing 14. The shape of the groove 132 matches the shape of the protrusion, and the groove 132 is also trapezoidal. When the protrusion and the groove 132 are matched, the end with the smaller outer diameter of the protrusion is joined first, which makes the joining easier and the engagement more stable. It is understood that the protrusion can also be a square block, a triangular prism block, etc.

[0027] One side of the fastener 13 is provided with multiple slots 131, which pass through the bottom and top ends of the fastener 13 and have an opening on the side facing the center of the housing 14. The multiple slots 131 are distributed along the length of the fastener 13, and the first clamping plate 11 and the second clamping plate 12 that hold the sheet material 20 can be jointly engaged in the slots 131. Since the fastener 13 is located on opposite sides of the housing 14, the first clamping plate 11 and the second clamping plate 12 that hold the sheet material 20 can be clamped and fixed by the fastener 13 located on opposite sides of the housing 14. In specific operation, the first clamping plate 11 and the second clamping plate 12 that hold the sheet material 20 are inserted from the top end of the fastener 13 into the slots 131, and the first clamping plate 11 and the second clamping plate 12 are engaged by the slots 131 to keep the sheet material 20 clamped.

[0028] For example, both the first clamping plate 11 and the second clamping plate 12 are polytetrafluoroethylene (PTFE) plates. PTFE has the advantages of heat and cold resistance, and can be used for a long time at temperatures ranging from -180 to 260°C; it also has the characteristics of acid and alkali resistance and organic solvent resistance. At the same time, PTFE has an extremely low coefficient of friction, which prevents it from sticking. When immersed in decellularization reagents, it is unlikely to react with the decellularization reagents, nor will it adhere to thin sheet tissues 20 such as SIS. This allows the decellularized sheet tissue 20 to be easily removed.

[0029] To ensure that the sheet material 20 can be securely clamped between the first clamping plate 11 and the second clamping plate 12, for example, at least one surface of the slot 131 for clamping the first clamping plate 11 and the second clamping plate 12 is provided with a spring piece 133 (see...). Figure 6 The upper end of the spring piece 133 is connected to the side wall of the slot 131, and there is a gap between the lower end and the side wall of the slot 131. For example, one end of the spring piece 133 has a connecting part, which is connected and fixed to the side wall of the slot 131 by a screw.

[0030] When the lower end of the spring piece 133 is compressed, it can move towards the side wall of the slot 131. When the first clamping plate 11 and the second clamping plate 12 are engaged in the slot 131, the spring piece 133 can abut against the surface of the first clamping plate 11 and / or the second clamping plate 12. When both side walls of the slot 131 are connected to the spring piece 133, the two spring pieces 133 can abut against the surface of the first clamping plate 11 and the second clamping plate 12 respectively. When one side wall of the slot 131 is connected to the spring piece 133, the spring piece 133 can abut against the surface of the first clamping plate 11 or the surface of the second clamping plate 12.

[0031] When the spring piece 133 abuts against the surface of the first clamping plate 11 and / or the second clamping plate 12, it can apply an elastic force to the first clamping plate 11 and / or the second clamping plate 12, so that the first clamping plate 11 and the second clamping plate 12 can clamp each other more tightly, thereby making the sheet structure 20 less prone to deformation and curling.

[0032] In summary, in the sheet tissue decellularization assembly 10 of this application embodiment, after the first clamping plate 11 and the second clamping plate 12 clamp the sheet tissue 20, the first clamping plate 11 and the second clamping plate 12 can be jointly engaged in the slot 131 of the fixing member 13. The first clamping plate 11 and the second clamping plate 12 are fixed in the box body 14 by the fixing members 13 fixed to opposite sides of the box body 14, which avoids the sheet tissue 20 from curling and achieves a better decellularization effect. Moreover, the fixing member 13 is detachably connected to the box body 14, and the first clamping plate 11 and the second clamping plate 12 are engaged in the slot 131 of the fixing member 13, so it can be easily assembled and disassembled, can easily clamp the sheet tissue 20, and can easily remove the decellularized sheet tissue 20 without damage.

[0033] The above are merely preferred embodiments of this application and are not intended to limit this application. Various modifications and variations can be made to this application by those skilled in the art. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of this application should be included within the protection scope of this application.

Claims

1. A sheet tissue decellularization assembly, characterized in that, include: The first clamping plate and the second clamping plate are used to clamp the sheet material; the middle of the first clamping plate and the second clamping plate are hollowed out, and the plate body has through holes along the thickness direction; The fastener has multiple slots on one side, which are distributed along the length of the fastener. One end of the first clamping plate and the second clamping plate, which hold the sheet material, can be engaged together in these slots. The box is used to hold decellularization reagents. The fixing member is detachably connected to the box and can be fixed to opposite sides inside the box. The distance between the fixing members on opposite sides is less than the length of the first clamping plate and the second clamping plate.

2. The sheet tissue decellularization assembly according to claim 1, characterized in that, The first clamping plate has a first through hole, and the second clamping plate has a second through hole, with the first through hole and the second through hole being staggered.

3. The sheet tissue decellularization assembly according to claim 1, characterized in that, The fastener is connected and fixed to the bottom wall of the box by a snap-fit ​​method.

4. The sheet tissue decellularization assembly according to claim 3, characterized in that, The bottom wall of the box is provided with a protrusion, and the bottom end of the fastener is provided with a groove that matches the protrusion, and the protrusion can be engaged in the groove.

5. The sheet tissue decellularization assembly according to claim 4, characterized in that, The protrusion is trapezoidal, and its outer diameter gradually increases from the end away from the bottom wall of the box to the end closer to the bottom wall of the box.

6. The sheet tissue decellularization assembly according to claim 3, characterized in that, The slot is used to clamp at least one surface of the first clamping plate and the second clamping plate, and a spring piece is provided thereon; one end of the spring piece is connected to the side wall of the slot, and the other end is spaced apart from the side wall of the slot; when the first clamping plate and the second clamping plate are engaged in the slot, the spring piece can abut against the surface of the first clamping plate and / or the second clamping plate.

7. The sheet tissue decellularization assembly according to claim 6, characterized in that, The upper end of the spring is connected to the side wall of the slot.

8. The sheet tissue decellularization assembly according to any one of claims 1 to 6, characterized in that, Both the first clamping plate and the second clamping plate are polytetrafluoroethylene (PTFE) plates.

Images