A nucleic acid purification and nucleic acid amplification device

Abstract

This utility model relates to the field of nucleic acid detection technology, specifically a nucleic acid purification and amplification device, comprising: a sample chamber including a lid, a flow channel, and a sample compartment; a filter membrane that can cooperate with the sample chamber to form a filter structure for purifying samples; an amplification sealing plug for controlling the connection between the sample chamber and the amplification module, with the sample flowing from the sample chamber into the amplification sealing plug and into the amplification module; an amplification module including a flow channel and an amplification chamber; a support base for combining with the amplification module; and an expansion ball that rapidly absorbs water and expands after the sample enters the amplification chamber through the flow channel, blocking the flow channel. After the sample is added to the sample chamber, by pressing down on the sample chamber and puncturing the amplification sealing ring, the sample in the sample chamber is controlled by the negative pressure within the amplification module to enter the amplification chamber from the sample chamber. The sample amplification chamber is used to detect the sample in the amplification chamber to obtain the sample detection results, meeting the portability requirements of medical testing, and is convenient and fast to operate.

Description

Technical Field

[0001] This utility model relates to the field of nucleic acid detection technology, and in particular to a nucleic acid purification and nucleic acid amplification device. Background Technology

[0002] Existing microfluidic cartridges, microfluidic chips, and other commonly used products suffer from numerous consumable components and complex assembly processes. This not only increases production costs but also raises the risk of errors during production. In addition, these products are bulky, lack portability, and are difficult to adapt to diverse testing scenarios.

[0003] In terms of flow channel control, existing technologies mostly employ rotary valve control, mechanical valve control, and paraffin valve control. These control methods require extremely high precision control from the supporting instruments and have cumbersome operating procedures. This not only increases the difficulty and cost of instrument development but also limits detection efficiency to some extent. In practical applications, they often fail to meet the needs for rapid, convenient, and accurate detection. Therefore, we need to upgrade and modify existing technologies to overcome existing problems and shortcomings. Utility Model Content

[0004] The purpose of this invention is to provide a nucleic acid purification and nucleic acid amplification device to solve the problems mentioned in the background art.

[0005] To achieve the above objectives, this utility model provides the following technical solution:

[0006] Design a nucleic acid purification and nucleic acid amplification device, including a nucleic acid amplification cartridge. The nucleic acid amplification cartridge includes a sample chamber, a filter membrane, an amplification sealing ring, an expansion ball, an amplification module, and a support base. The sample chamber is fitted with the amplification sealing ring, the filter membrane is disposed inside the sample chamber, the amplification sealing ring is sealed with the amplification module, the expansion ball is located in the ball groove of the amplification module, and the amplification module is combined with the support base.

[0007] Preferably, the sample chamber includes a lid, an inlet, a filter membrane groove, a first flow channel, a first locking position, a second locking position, a ejector pin, and a first positioning groove. The lid cooperates with the inlet, the filter membrane groove is used to place the filter membrane, the first flow channel connects the inside of the sample chamber to the amplification module, the ejector pin corresponds to the amplification sealing ring, and the first positioning groove is connected to the amplification module.

[0008] Preferably, the cap and the injection port are opened and closed by an interference fit, and the injection port receives the sampling swab or sample solution.

[0009] Preferably, the amplified sealing ring includes an upper sealing groove, a lower sealing groove, a first contact surface, a sealing ring, a second contact surface, and a diaphragm. The upper sealing groove is initially fitted and sealed with the ejector pin. The lower end of the upper sealing groove is provided with a diaphragm, and the lower end of the diaphragm is provided with a lower sealing groove.

[0010] Preferably, the amplification module includes a second positioning groove, an exhaust groove, a third contact surface, a second flow channel, a ball groove, a third locking position, a support column, and an amplification chamber. The second positioning groove cooperates with the first positioning groove, the second flow channel connects the ball groove and the amplification chamber, the expansion ball is placed in the ball groove, the support column is combined with the lower sealing groove, and the amplification chamber is pre-embedded with lyophilized reagents and paraffin balls.

[0011] Preferably, the second contact surface is bonded to the third contact surface, and the sealing ring is bonded and sealed to the inside of the amplification module.

[0012] Preferably, the first card slot, the second card slot, and the third card slot of the amplification module are engaged, and the first card slot and the third card slot are attached after assembly. The third card slot can be moved up to engage with the second card slot.

[0013] Preferably, the amplification module can be configured with one, two, three, four or more amplification chambers to meet the needs of multiple reagent reactions.

[0014] Compared with the prior art, the beneficial effects of this utility model are as follows:

[0015] 1. This utility model has a sample chamber and an amplification module inside the device. The sample chamber and the amplification module are connected by a first locking position, a second locking position and a third locking position, and a first positioning groove and a second positioning groove. When pressed, the third locking position moves up to the second locking position, and the positioning is accurate and stable. After the ejector pin punctures the diaphragm, it is sealed with an expansion ball. The connection has both sealing and controllability, making the operation convenient and ensuring efficient and reliable sample transmission.

[0016] 2. This utility model features an amplification sealing ring and an amplification module within the device. The amplification module and the amplification sealing ring utilize negative pressure to preserve reagents, maintaining their activity. The combination provides a tight seal, reducing the risk of contamination. Sample injection is driven by negative pressure, and the structural design enables automatic and precise transfer, improving operational efficiency and detection reliability.

[0017] 3. This utility model has a device with a needle and an expansion ball inside. After the needle punctures the diaphragm, the expansion ball absorbs water and expands to form a valve-type flow channel seal, which can block sample backflow and external contamination. Combined with negative pressure driven sample transmission, it not only ensures the flow channel sealing, but also improves the accuracy and stability of nucleic acid amplification detection.

[0018] Specific embodiments of the present invention are disclosed in detail with reference to the following description and accompanying drawings, indicating how the principles of the present invention can be employed. It should be understood that the embodiments of the present invention are not limited in scope. Within the spirit and scope of the appended claims, the embodiments of the present invention include many changes, modifications, and equivalents. Attached Figure Description

[0019] The accompanying drawings are provided to further illustrate the present invention and form part of the specification. They are used together with the embodiments of the present invention to explain the present invention, but do not constitute a limitation thereof. In the drawings:

[0020] Figure 1 This is a schematic diagram of the overall structure according to the present utility model;

[0021] Figure 2 This is an exploded view of the overall structure according to this utility model;

[0022] Figure 3 This is a cross-sectional view of the internal structure according to the present invention;

[0023] Figure 4 A cross-sectional view of the nucleic acid amplification cartridge according to this utility model;

[0024] Figure 5 This is a top view of the amplification module according to this utility model.

[0025] In the diagram: 1. Nucleic acid amplification cartridge; 10. Sample chamber; 11. Lid; 12. Inlet; 13. Filter membrane groove; 14. First flow channel; 15. First locking position; 16. Second locking position; 17. Ejector pin; 18. First positioning groove; 20. Filter membrane; 30. Amplification sealing ring; 31. Upper sealing groove; 32. Lower sealing groove; 33. First contact surface; 34. Sealing ring; 35. Second contact surface; 36. Diaphragm; 40. Expansion ball; 50. Amplification module; 51. Second positioning groove; 52. Exhaust groove; 53. Third contact surface; 54. Second flow channel; 55. Ball groove; 56. Third locking position; 57. Support column; 58. Amplification chamber; 60. Support base. Detailed Implementation

[0026] To make the technical means, creative features, objectives and effects of this utility model easier to understand, the present utility model will be further described below in conjunction with specific embodiments.

[0027] like Figure 1-5As shown, this embodiment provides a nucleic acid purification and amplification device, including a nucleic acid amplification cartridge 1. The nucleic acid amplification cartridge 1 includes a sample chamber 10, a filter membrane 20, an amplification sealing ring 30, an expansion ball 40, an amplification module 50, and a support base 60. The sample chamber 10 is fitted with the amplification sealing ring 30, the filter membrane 20 is disposed in the sample chamber 10, the amplification sealing ring 30 is sealed with the amplification module 50, the expansion ball 40 is located in the ball groove 55 of the amplification module 50, and the amplification module 50 is combined with the support base 60.

[0028] In this embodiment, the sample chamber 10 includes a cover 11, an inlet 12, a filter membrane groove 13, a first flow channel 14, a first locking position 15, a second locking position 16, a ejector pin 17, and a first positioning groove 18. The cover 11 mates with the inlet 12. The filter membrane groove 13 is used to place the filter membrane 20. The first flow channel 14 connects the inside of the sample chamber 10 to the amplification module 50. The ejector pin 17 corresponds to the amplification sealing ring 30. The first positioning groove 18 is connected to the amplification module 50. The first locking position 15 and the second locking position 16 mate with the third locking position 56 of the amplification module 50. After assembly, the first locking position 15 and the third locking position 56 fit together. The third locking position 56 can be moved upward to mate with the second locking position 16. The cover 11 and the inlet 12 are opened and closed by an interference fit. The inlet 12 receives a sampling swab or sample solution. The sample chamber receives a sampling swab or sample solution. The sample is placed in by opening the cap 11. After closing the cap, the sample chamber 10 is pressed to change the locking mechanism. The ejector pin 17 punctures the diaphragm 36, and the sample enters the amplification module 50 through the flow channel. The sample chamber 10 and the amplification module 50 are connected by locking and positioning slots, ensuring precise and stable assembly. The operation is simple, making sample transfer efficient and meeting the needs of portable and rapid medical testing.

[0029] In this embodiment, the amplification sealing ring 30 includes an upper sealing groove 31, a lower sealing groove 32, a first contact surface 33, a sealing ring 34, a second contact surface 35, and a diaphragm 36. The upper sealing groove 31 is initially fitted and sealed with the ejector pin 17. The lower end of the upper sealing groove 31 is provided with the diaphragm 36, and the lower end of the diaphragm 36 is provided with the lower sealing groove 32. The second contact surface 35 is fitted with the third contact surface 53. The sealing ring 34 is fitted and sealed with the inside of the amplification module 50. After the ejector pin 17 punctures the diaphragm 36, it enters the lower sealing groove 32, realizing the connection between the sample chamber 10 and the amplification module 50. At the same time, the sealing ring 34 ensures a seal to prevent leakage, realizing the controllable connection between the sample chamber 10 and the amplification module 50. The amplification module 50 and the sealing plug preserve the reagent under negative pressure. The combination and sealing are good, the sample injection is controllable, the contamination is reduced, the detection accuracy is improved, and it is suitable for rapid detection scenarios.

[0030] In this embodiment, the amplification module 50 includes a second positioning groove 51, an exhaust groove 52, a third contact surface 53, a second flow channel 54, a ball groove 55, a third locking position 56, a support column 57, and an amplification chamber 58. The second positioning groove 51 cooperates with the first positioning groove 18. The second flow channel 54 connects the ball groove 55 and the amplification chamber 58. The expansion ball 40 is placed in the ball groove 55. The support column 57 is combined with the lower sealing groove 32. The amplification chamber 58 is pre-embedded with lyophilized reagents and paraffin balls. The amplification module 50 can be set with one, two, three, four, or more amplification chambers 58 to meet the needs of multiple reagent reactions. The sample is drawn into the flow channel by negative pressure. The expansion ball 40 absorbs water and expands to seal the flow channel. After the sample dissolves the reagent in the amplification chamber 58, it is amplified and detected. After the diaphragm 36 is punctured by the tip 17, the expansion ball 40 expands and seals the flow channel to prevent sample backflow, protect the amplification environment, and make the detection efficient, reliable, and convenient to operate.

[0031] The working principle and process of this utility model are as follows: First, open the lid 11, place the sampling swab or sample solution into the sample chamber 10, and then close the lid 11. Press the sample chamber 10 to move the third locking position 56 upwards to engage with the second locking position 16. At this time, the ejector pin 17 of the sample chamber 10 pierces the diaphragm 36 of the amplification sealing ring 30 and enters the lower sealing groove 32. The lysed sample, under the negative pressure suction of the amplification chamber 58, flows to the filter membrane 20 for impurity purification, then enters the ball groove 55 through the first flow channel 14, and subsequently flows along the second flow channel 54 to the amplification chamber 58. Simultaneously, the expansion ball 40 in the ball groove 55 absorbs moisture from the sample and expands, contacting the ejector pin 17 to seal the first flow channel 14. After the sample enters the amplification chamber 58, the lyophilized ball dissolves. Then, the nucleic acid amplification cartridge 1 is placed in the matching instrument for nucleic acid amplification detection. The entire process is completed efficiently through the precise cooperation of each component.

[0032] In the description of this utility model, it should be noted that, unless otherwise explicitly specified and limited, the terms "installed," "equipped with," "connected," etc., should be interpreted broadly. For example, "connection" can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be a direct connection or an indirect connection through an intermediate medium; it can be a connection within two components. Those skilled in the art can understand the specific meaning of the above terms in this utility model according to the specific circumstances.

[0033] In the description of this embodiment, the terms "upper," "lower," "right," etc., refer to the orientation or positional relationship shown in the accompanying drawings. They are used only for ease of description and simplification of operation, and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation. Therefore, they should not be construed as limitations on this utility model. In addition, the terms "first" and "second" are only used for distinction in description and have no special meaning.

Claims

1. A nucleic acid purification and nucleic acid amplification device, characterized by, The device includes a nucleic acid amplification cartridge (1), which includes a sample chamber (10), a filter membrane (20), an amplification sealing ring (30), an expansion ball (40), an amplification module (50), and a support base (60). The sample chamber (10) is fitted with the amplification sealing ring (30), the filter membrane (20) is disposed in the sample chamber (10), the amplification sealing ring (30) is sealed with the amplification module (50), the expansion ball (40) is located in the ball groove (55) of the amplification module (50), and the amplification module (50) is combined with the support base (60).

2. The nucleic acid purification and nucleic acid amplification device according to claim 1, characterized by: The sample chamber (10) includes a cover (11), an inlet (12), a filter membrane groove (13), a first flow channel (14), a first locking position (15), a second locking position (16), a push pin (17), and a first positioning groove (18). The cover (11) is fitted with the inlet (12). The filter membrane groove (13) is used to place the filter membrane (20). The first flow channel (14) connects the inside of the sample chamber (10) with the amplification module (50). The push pin (17) corresponds to the amplification sealing ring (30). The first positioning groove (18) is connected to the amplification module (50).

3. A nucleic acid purification and nucleic acid amplification device according to claim 2, wherein: The cap (11) and the inlet (12) are opened and closed by an interference fit, and the inlet (12) receives the sampling swab or sample solution.

4. The nucleic acid purification and nucleic acid amplification device of claim 2, wherein: The amplified sealing ring (30) includes an upper sealing groove (31), a lower sealing groove (32), a first contact surface (33), a sealing ring (34), a second contact surface (35), and a diaphragm (36). The upper sealing groove (31) is initially fitted and sealed with the ejector pin (17). The lower end of the upper sealing groove (31) is provided with a diaphragm (36), and the lower end of the diaphragm (36) is provided with a lower sealing groove (32).

5. A nucleic acid purification and nucleic acid amplification device according to claim 4, wherein: The amplification module (50) includes a second positioning groove (51), an exhaust groove (52), a third contact surface (53), a second flow channel (54), a ball groove (55), a third locking position (56), a support column (57), and an amplification chamber (58). The second positioning groove (51) cooperates with the first positioning groove (18). The second flow channel (54) connects the ball groove (55) and the amplification chamber (58). The expansion ball (40) is placed in the ball groove (55). The support column (57) is combined with the lower sealing groove (32). The amplification chamber (58) is pre-embedded with lyophilized reagents and paraffin balls.

6. A nucleic acid purification and nucleic acid amplification device according to claim 5, wherein: The second contact surface (35) is bonded to the third contact surface (53), and the sealing ring (34) is bonded and sealed to the inside of the amplification module (50).

7. The nucleic acid purification and nucleic acid amplification device of claim 5, wherein: The first card slot (15) and the second card slot (16) cooperate with the third card slot (56) of the amplification module (50). After the first card slot (15) and the third card slot (56) are assembled, they are attached together. The third card slot (56) can be moved up to cooperate with the second card slot (16).

8. The nucleic acid purification and nucleic acid amplification device of claim 5, wherein: The amplification module (50) can be configured with one, two, three, four or more amplification chambers (58) to meet the needs of multiple reagent reactions.

Images